Journal of Immunological Methods This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation
J Immunol Methods. 2023 Aug 18:113537. doi: 10.1016/j.jim.2023.113537. Online ahead of print.ABSTRACTTransplantation of organs, cells, or tissues from one species to another, known as xenotransplantation, has the potential to alleviate organ donor shortages and enhance the success of organ transplantation. However, the possibility of immunological rejection by the recipient is one of the biggest difficulties associated with xenotransplantation. The creation of neutrophil extracellular traps (NETs), also known as NETosis, is hypothesized as a mechanism of rejection. Innovations in microfluidics and co-culturing techniques h...
Source: Journal of Immunological Methods - August 20, 2023 Category: Allergy & Immunology Authors: Suchen Kumar Yadav Seokwoo Park Yun-Mi Lee Sunghoon Hurh Dongsuk Kim Sangil Min Sejoong Kim Ji-Jing Yan Byeong-Cheol Kang Sungjoo Kim Jaeseok Yang Jong Cheol Jeong Source Type: research

A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca ® PLX image cytometer
J Immunol Methods. 2023 Aug 17:113538. doi: 10.1016/j.jim.2023.113538. Online ahead of print.ABSTRACTIn cellular therapies chimeric antigen receptor (CAR) T or NK cells undergo phenotypic analysis at multiple stages during discovery and development of novel therapies. Patient samples are routinely analyzed via flow cytometry for population identification and distribution of CD3, CD4, and CD8 positive T cells. As an alternative or orthogonal method, image cytometry systems have been used to perform simple cell-based assays in lieu of flow cytometry. Recently, a new image cytometry system, the Cellaca PLX (Revvity Health Sci...
Source: Journal of Immunological Methods - August 19, 2023 Category: Allergy & Immunology Authors: Carolina Franco Nitta Mackenzie Pierce Jeanne Elia Jen Ruiz Art-Danniel Hipol Nicholas Fong Henry Qazi Sarah Kessel Dmitry Kuksin Eunice Mejia Bo Lin Timothy Smith Josh Croteau Nicolas Schrantz Xifeng Yang Leo Li-Ying Chan Source Type: research

MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation
J Immunol Methods. 2023 Aug 17:113540. doi: 10.1016/j.jim.2023.113540. Online ahead of print.ABSTRACTKnowing that an antibody's sensitivity and specificity is accurate is crucial for reliable data collection. This certainty is especially difficult to achieve for antibodies (Abs) which bind post-translationally modified proteins. Here we describe two validation methods using surrogate proteins in western blot and ELISA. The first method, which we termed "MILKSHAKE" is a modified maltose binding protein, hence the name, that is enzymatically conjugated to a peptide from the chosen target which is either modified or non-modif...
Source: Journal of Immunological Methods - August 19, 2023 Category: Allergy & Immunology Authors: Qiana Mendez Holland A Driscoll Gregory R Mirando Felicity Acca Cassandra D Chapados Kezzia S Jones Michael Weiner Xiaofeng Li Mary R Ferguson Source Type: research

Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry
J Immunol Methods. 2023 Aug 16:113539. doi: 10.1016/j.jim.2023.113539. Online ahead of print.ABSTRACTThe multivalent pneumococcal conjugate vaccine (PCV) contains purified polysaccharides of different serotypes conjugated to a carrier protein. Testing the final formulated product for individual serotype polysaccharide content is critical in vaccine quality control which requires an assay specific to each serotype polysaccharide present in the formulated product. Antibodies specific to the serotypes specific polysaccharide were used in rate nephelometry assay for quantifying individual serotype polysaccharides in the formul...
Source: Journal of Immunological Methods - August 18, 2023 Category: Allergy & Immunology Authors: Burki Rajendar Jaya Sheela Pydigummala Ganti Sreenivasa Rao Ramakrishna Chigurambotla Ramesh V Matur Source Type: research

Performance comparison of three commercial tests for the detection of SARS-CoV-2 antibodies in a common set of pediatric samples
CONCLUSIONS: Our findings suggest good performance of commercial SARS-CoV-2 IgG assays in pediatric patients with samples collected ≥15 days PSO. Additional studies investigating antibody response and assay performance in children are warranted.PMID:37567470 | DOI:10.1016/j.jim.2023.113536 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - August 11, 2023 Category: Allergy & Immunology Authors: Sophonie J Oyeniran Huanyu Wang Kathy Everhart Kathy Mack Kevin Harvey Amy L Leber Source Type: research

Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure
J Immunol Methods. 2023 Aug 7:113535. doi: 10.1016/j.jim.2023.113535. Online ahead of print.ABSTRACTLow pH stress and its influence on antibody binding is a common consideration among chemists, but is only recently emerging as a consideration in Immunological studies. Antibody characterizations in Multiple Sclerosis (MS), an autoimmune disease of the Central Nervous System (CNS) has revealed that antibodies in the cerebrospinal fluid (CSF) of patients with Multiple Sclerosis bind to myelin-related and non-myelin antigen targets. Many laboratories have used molecular biology techniques to generate recombinant human antibodi...
Source: Journal of Immunological Methods - August 9, 2023 Category: Allergy & Immunology Authors: Wei Zhang Chaitanya Joshi Chad Smith Thomas A Ujas Jacqueline R Rivas Lindsay Cowell Scott Christley Ann M Stowe Nancy L Monson Source Type: research

Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis
CONCLUSION: This study successfully established a highly sensitive ST2-TRFIA, which was highly comparable to commercially available fluorescent quantitative immunochromatographic kits and can facilitate the timely diagnosis of sepsis.PMID:37558124 | DOI:10.1016/j.jim.2023.113534 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - August 9, 2023 Category: Allergy & Immunology Authors: Lu Wang Benqi Fu Zhongyi Xiang Xindong Chen Jianye Chen Yuan Qin Huiming Sheng Xiumei Zhou Qian Li Biao Huang Source Type: research