Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN- ɣ enzyme-linked ImmunoSpot (ELISpot) protocols
In conclusion, proficiency tests show that a limited harmonization effort consisting of the introduction of SOPs and the use of the same in vitro stimulating antigens leads to a reduction of the interlaboratory variation of IFN-ɣ ELISpot data and demonstrate that substantial improvements for the ICS assay are achieved as comparable laboratory datasets could be generated. Additional steps to further reduce the interlaboratory variation of ICS data can consist of standardized gating templates and detailed data reporting instructions as well as further efforts to harmonize reagent and instrument use.PMID:37918618 | DOI:10.10...
Source: Journal of Immunological Methods - November 2, 2023 Category: Allergy & Immunology Authors: Gwenn Waerlop Geert Leroux-Roels Anke Pagnon Sarah Begue Bruno Salaun Michel Janssens Donata Medaglini Elena Pettini Emanuele Montomoli Elena Gianchecchi Teresa Lambe Leila Godfrey Maireid Bull Duncan Bellamy H åkon Amdam Geir Bredholt Rebecca Jane Cox F Source Type: research
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN- ɣ enzyme-linked ImmunoSpot (ELISpot) protocols
In conclusion, proficiency tests show that a limited harmonization effort consisting of the introduction of SOPs and the use of the same in vitro stimulating antigens leads to a reduction of the interlaboratory variation of IFN-ɣ ELISpot data and demonstrate that substantial improvements for the ICS assay are achieved as comparable laboratory datasets could be generated. Additional steps to further reduce the interlaboratory variation of ICS data can consist of standardized gating templates and detailed data reporting instructions as well as further efforts to harmonize reagent and instrument use.PMID:37918618 | DOI:10.10...
Source: Journal of Immunological Methods - November 2, 2023 Category: Allergy & Immunology Authors: Gwenn Waerlop Geert Leroux-Roels Anke Pagnon Sarah Begue Bruno Salaun Michel Janssens Donata Medaglini Elena Pettini Emanuele Montomoli Elena Gianchecchi Teresa Lambe Leila Godfrey Maireid Bull Duncan Bellamy H åkon Amdam Geir Bredholt Rebecca Jane Cox F Source Type: research
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN- ɣ enzyme-linked ImmunoSpot (ELISpot) protocols
In conclusion, proficiency tests show that a limited harmonization effort consisting of the introduction of SOPs and the use of the same in vitro stimulating antigens leads to a reduction of the interlaboratory variation of IFN-ɣ ELISpot data and demonstrate that substantial improvements for the ICS assay are achieved as comparable laboratory datasets could be generated. Additional steps to further reduce the interlaboratory variation of ICS data can consist of standardized gating templates and detailed data reporting instructions as well as further efforts to harmonize reagent and instrument use.PMID:37918618 | DOI:10.10...
Source: Journal of Immunological Methods - November 2, 2023 Category: Allergy & Immunology Authors: Gwenn Waerlop Geert Leroux-Roels Anke Pagnon Sarah Begue Bruno Salaun Michel Janssens Donata Medaglini Elena Pettini Emanuele Montomoli Elena Gianchecchi Teresa Lambe Leila Godfrey Maireid Bull Duncan Bellamy H åkon Amdam Geir Bredholt Rebecca Jane Cox F Source Type: research
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN- ɣ enzyme-linked ImmunoSpot (ELISpot) protocols
In conclusion, proficiency tests show that a limited harmonization effort consisting of the introduction of SOPs and the use of the same in vitro stimulating antigens leads to a reduction of the interlaboratory variation of IFN-ɣ ELISpot data and demonstrate that substantial improvements for the ICS assay are achieved as comparable laboratory datasets could be generated. Additional steps to further reduce the interlaboratory variation of ICS data can consist of standardized gating templates and detailed data reporting instructions as well as further efforts to harmonize reagent and instrument use.PMID:37918618 | DOI:10.10...
Source: Journal of Immunological Methods - November 2, 2023 Category: Allergy & Immunology Authors: Gwenn Waerlop Geert Leroux-Roels Anke Pagnon Sarah Begue Bruno Salaun Michel Janssens Donata Medaglini Elena Pettini Emanuele Montomoli Elena Gianchecchi Teresa Lambe Leila Godfrey Maireid Bull Duncan Bellamy H åkon Amdam Geir Bredholt Rebecca Jane Cox F Source Type: research
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN- ɣ enzyme-linked ImmunoSpot (ELISpot) protocols
In conclusion, proficiency tests show that a limited harmonization effort consisting of the introduction of SOPs and the use of the same in vitro stimulating antigens leads to a reduction of the interlaboratory variation of IFN-ɣ ELISpot data and demonstrate that substantial improvements for the ICS assay are achieved as comparable laboratory datasets could be generated. Additional steps to further reduce the interlaboratory variation of ICS data can consist of standardized gating templates and detailed data reporting instructions as well as further efforts to harmonize reagent and instrument use.PMID:37918618 | DOI:10.10...
Source: Journal of Immunological Methods - November 2, 2023 Category: Allergy & Immunology Authors: Gwenn Waerlop Geert Leroux-Roels Anke Pagnon Sarah Begue Bruno Salaun Michel Janssens Donata Medaglini Elena Pettini Emanuele Montomoli Elena Gianchecchi Teresa Lambe Leila Godfrey Maireid Bull Duncan Bellamy H åkon Amdam Geir Bredholt Rebecca Jane Cox F Source Type: research
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN- ɣ enzyme-linked ImmunoSpot (ELISpot) protocols
In conclusion, proficiency tests show that a limited harmonization effort consisting of the introduction of SOPs and the use of the same in vitro stimulating antigens leads to a reduction of the interlaboratory variation of IFN-ɣ ELISpot data and demonstrate that substantial improvements for the ICS assay are achieved as comparable laboratory datasets could be generated. Additional steps to further reduce the interlaboratory variation of ICS data can consist of standardized gating templates and detailed data reporting instructions as well as further efforts to harmonize reagent and instrument use.PMID:37918618 | DOI:10.10...
Source: Journal of Immunological Methods - November 2, 2023 Category: Allergy & Immunology Authors: Gwenn Waerlop Geert Leroux-Roels Anke Pagnon Sarah Begue Bruno Salaun Michel Janssens Donata Medaglini Elena Pettini Emanuele Montomoli Elena Gianchecchi Teresa Lambe Leila Godfrey Maireid Bull Duncan Bellamy H åkon Amdam Geir Bredholt Rebecca Jane Cox F Source Type: research
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A
J Immunol Methods. 2023 Oct 24:113574. doi: 10.1016/j.jim.2023.113574. Online ahead of print.ABSTRACTSerum amyloid A (SAA) is an acute-phase protein produced in response to inflammatory proteins during infections, inflammation, trauma, surgery, cancer, and other conditions. Early and accurate detection of SAA is necessary for diagnosis and monitoring of disease progression. To meet this need, we developed a gradient lateral flow immunoassay test strip using Au nanoparticles as signal reporters. The test strip has three test (T1, T2, and T3) lines with progressively decreasing concentrations of SAA antibody, enabling the de...
Source: Journal of Immunological Methods - October 26, 2023 Category: Allergy & Immunology Authors: Guangtian Yang Jishun Li Shenglan Zhang Huixiang Ouyang Chunhai Jiang Hongcheng Pan Source Type: research
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A
J Immunol Methods. 2023 Oct 24:113574. doi: 10.1016/j.jim.2023.113574. Online ahead of print.ABSTRACTSerum amyloid A (SAA) is an acute-phase protein produced in response to inflammatory proteins during infections, inflammation, trauma, surgery, cancer, and other conditions. Early and accurate detection of SAA is necessary for diagnosis and monitoring of disease progression. To meet this need, we developed a gradient lateral flow immunoassay test strip using Au nanoparticles as signal reporters. The test strip has three test (T1, T2, and T3) lines with progressively decreasing concentrations of SAA antibody, enabling the de...
Source: Journal of Immunological Methods - October 26, 2023 Category: Allergy & Immunology Authors: Guangtian Yang Jishun Li Shenglan Zhang Huixiang Ouyang Chunhai Jiang Hongcheng Pan Source Type: research
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A
J Immunol Methods. 2023 Oct 24:113574. doi: 10.1016/j.jim.2023.113574. Online ahead of print.ABSTRACTSerum amyloid A (SAA) is an acute-phase protein produced in response to inflammatory proteins during infections, inflammation, trauma, surgery, cancer, and other conditions. Early and accurate detection of SAA is necessary for diagnosis and monitoring of disease progression. To meet this need, we developed a gradient lateral flow immunoassay test strip using Au nanoparticles as signal reporters. The test strip has three test (T1, T2, and T3) lines with progressively decreasing concentrations of SAA antibody, enabling the de...
Source: Journal of Immunological Methods - October 26, 2023 Category: Allergy & Immunology Authors: Guangtian Yang Jishun Li Shenglan Zhang Huixiang Ouyang Chunhai Jiang Hongcheng Pan Source Type: research
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A
J Immunol Methods. 2023 Oct 24:113574. doi: 10.1016/j.jim.2023.113574. Online ahead of print.ABSTRACTSerum amyloid A (SAA) is an acute-phase protein produced in response to inflammatory proteins during infections, inflammation, trauma, surgery, cancer, and other conditions. Early and accurate detection of SAA is necessary for diagnosis and monitoring of disease progression. To meet this need, we developed a gradient lateral flow immunoassay test strip using Au nanoparticles as signal reporters. The test strip has three test (T1, T2, and T3) lines with progressively decreasing concentrations of SAA antibody, enabling the de...
Source: Journal of Immunological Methods - October 26, 2023 Category: Allergy & Immunology Authors: Guangtian Yang Jishun Li Shenglan Zhang Huixiang Ouyang Chunhai Jiang Hongcheng Pan Source Type: research
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A
J Immunol Methods. 2023 Oct 24:113574. doi: 10.1016/j.jim.2023.113574. Online ahead of print.ABSTRACTSerum amyloid A (SAA) is an acute-phase protein produced in response to inflammatory proteins during infections, inflammation, trauma, surgery, cancer, and other conditions. Early and accurate detection of SAA is necessary for diagnosis and monitoring of disease progression. To meet this need, we developed a gradient lateral flow immunoassay test strip using Au nanoparticles as signal reporters. The test strip has three test (T1, T2, and T3) lines with progressively decreasing concentrations of SAA antibody, enabling the de...
Source: Journal of Immunological Methods - October 26, 2023 Category: Allergy & Immunology Authors: Guangtian Yang Jishun Li Shenglan Zhang Huixiang Ouyang Chunhai Jiang Hongcheng Pan Source Type: research
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A
J Immunol Methods. 2023 Oct 24:113574. doi: 10.1016/j.jim.2023.113574. Online ahead of print.ABSTRACTSerum amyloid A (SAA) is an acute-phase protein produced in response to inflammatory proteins during infections, inflammation, trauma, surgery, cancer, and other conditions. Early and accurate detection of SAA is necessary for diagnosis and monitoring of disease progression. To meet this need, we developed a gradient lateral flow immunoassay test strip using Au nanoparticles as signal reporters. The test strip has three test (T1, T2, and T3) lines with progressively decreasing concentrations of SAA antibody, enabling the de...
Source: Journal of Immunological Methods - October 26, 2023 Category: Allergy & Immunology Authors: Guangtian Yang Jishun Li Shenglan Zhang Huixiang Ouyang Chunhai Jiang Hongcheng Pan Source Type: research
Assessment of mimicking by EBV-CMV immunoglobulin M of anti-HLA antibodies
CONCLUSION: Cross-reactivity may occur between anti-CMV and anti-EBV IgM antibodies with HLA molecules. Thus, in graft recipients, pathogenic IgMs can also act as de novo anti-HLA antibodies and aggravate the rejection process.PMID:37865308 | DOI:10.1016/j.jim.2023.113577 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - October 21, 2023 Category: Allergy & Immunology Authors: Rasime Derya G üleç Fatma Demet Arslan Berk Özyılmaz Nisel Y ılmaz Sevgi Y ılmaz Hancı Şükran Köse Source Type: research
Assessment of mimicking by EBV-CMV immunoglobulin M of anti-HLA antibodies
CONCLUSION: Cross-reactivity may occur between anti-CMV and anti-EBV IgM antibodies with HLA molecules. Thus, in graft recipients, pathogenic IgMs can also act as de novo anti-HLA antibodies and aggravate the rejection process.PMID:37865308 | DOI:10.1016/j.jim.2023.113577 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - October 21, 2023 Category: Allergy & Immunology Authors: Rasime Derya G üleç Fatma Demet Arslan Berk Özyılmaz Nisel Y ılmaz Sevgi Y ılmaz Hancı Şükran Köse Source Type: research
Assessment of mimicking by EBV-CMV immunoglobulin M of anti-HLA antibodies
CONCLUSION: Cross-reactivity may occur between anti-CMV and anti-EBV IgM antibodies with HLA molecules. Thus, in graft recipients, pathogenic IgMs can also act as de novo anti-HLA antibodies and aggravate the rejection process.PMID:37865308 | DOI:10.1016/j.jim.2023.113577 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - October 21, 2023 Category: Allergy & Immunology Authors: Rasime Derya G üleç Fatma Demet Arslan Berk Özyılmaz Nisel Y ılmaz Sevgi Y ılmaz Hancı Şükran Köse Source Type: research
