Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure
J Immunol Methods. 2023 Aug 7:113535. doi: 10.1016/j.jim.2023.113535. Online ahead of print.ABSTRACTLow pH stress and its influence on antibody binding is a common consideration among chemists, but is only recently emerging as a consideration in Immunological studies. Antibody characterizations in Multiple Sclerosis (MS), an autoimmune disease of the Central Nervous System (CNS) has revealed that antibodies in the cerebrospinal fluid (CSF) of patients with Multiple Sclerosis bind to myelin-related and non-myelin antigen targets. Many laboratories have used molecular biology techniques to generate recombinant human antibodi...
Source: Journal of Immunological Methods - August 9, 2023 Category: Allergy & Immunology Authors: Wei Zhang Chaitanya Joshi Chad Smith Thomas A Ujas Jacqueline R Rivas Lindsay Cowell Scott Christley Ann M Stowe Nancy L Monson Source Type: research

Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis
CONCLUSION: This study successfully established a highly sensitive ST2-TRFIA, which was highly comparable to commercially available fluorescent quantitative immunochromatographic kits and can facilitate the timely diagnosis of sepsis.PMID:37558124 | DOI:10.1016/j.jim.2023.113534 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - August 9, 2023 Category: Allergy & Immunology Authors: Lu Wang Benqi Fu Zhongyi Xiang Xindong Chen Jianye Chen Yuan Qin Huiming Sheng Xiumei Zhou Qian Li Biao Huang Source Type: research

Corrigendum to "A taxonomy of multiple regression methods for immunologists" [Journal of Immunological Methods (2023) pp. 1-6 / Article No. 113506]
J Immunol Methods. 2023 Aug 7:113533. doi: 10.1016/j.jim.2023.113533. Online ahead of print.NO ABSTRACTPMID:37558523 | DOI:10.1016/j.jim.2023.113533 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - August 9, 2023 Category: Allergy & Immunology Authors: Tyson H Holmes Source Type: research

Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure
J Immunol Methods. 2023 Aug 7:113535. doi: 10.1016/j.jim.2023.113535. Online ahead of print.ABSTRACTLow pH stress and its influence on antibody binding is a common consideration among chemists, but is only recently emerging as a consideration in Immunological studies. Antibody characterizations in Multiple Sclerosis (MS), an autoimmune disease of the Central Nervous System (CNS) has revealed that antibodies in the cerebrospinal fluid (CSF) of patients with Multiple Sclerosis bind to myelin-related and non-myelin antigen targets. Many laboratories have used molecular biology techniques to generate recombinant human antibodi...
Source: Journal of Immunological Methods - August 9, 2023 Category: Allergy & Immunology Authors: Wei Zhang Chaitanya Joshi Chad Smith Thomas A Ujas Jacqueline R Rivas Lindsay Cowell Scott Christley Ann M Stowe Nancy L Monson Source Type: research

Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis
CONCLUSION: This study successfully established a highly sensitive ST2-TRFIA, which was highly comparable to commercially available fluorescent quantitative immunochromatographic kits and can facilitate the timely diagnosis of sepsis.PMID:37558124 | DOI:10.1016/j.jim.2023.113534 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - August 9, 2023 Category: Allergy & Immunology Authors: Lu Wang Benqi Fu Zhongyi Xiang Xindong Chen Jianye Chen Yuan Qin Huiming Sheng Xiumei Zhou Qian Li Biao Huang Source Type: research

Corrigendum to "A taxonomy of multiple regression methods for immunologists" [Journal of Immunological Methods (2023) pp. 1-6 / Article No. 113506]
J Immunol Methods. 2023 Aug 7:113533. doi: 10.1016/j.jim.2023.113533. Online ahead of print.NO ABSTRACTPMID:37558523 | DOI:10.1016/j.jim.2023.113533 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - August 9, 2023 Category: Allergy & Immunology Authors: Tyson H Holmes Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17;520:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research

Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
J Immunol Methods. 2023 Jul 17:113525. doi: 10.1016/j.jim.2023.113525. Online ahead of print.ABSTRACTThe authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for ...
Source: Journal of Immunological Methods - July 19, 2023 Category: Allergy & Immunology Authors: Alina Alshevskaya Julia Zhukova Julia Lopatnikova Nadezhda Shkaruba Oksana Chumasova Alexey Sizikov Daria Demina Vera Nepomniashchikh Viktor Gladkikh Sergey Sennikov Source Type: research