Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study
J Immunol Methods. 2024 Mar 13:113665. doi: 10.1016/j.jim.2024.113665. Online ahead of print.ABSTRACTOn March 13, 2021, Tunisia started a widespread immunization program against SARS-CoV-2 utilizing different vaccinations that had been given emergency approval. Herein, we followed prospectively a cohort of participant who received COVID-19 vaccine (Pfizer BioNTech and Sputnik-Gameleya V). The goal of this follow-up was to define the humoral and cellular immunological profile after immunization by assessing neutralizing antibodies and IFN- γ release. 26 vaccinated health care workers by Pfizer BioNTech (n = 12) and Sputnik...
Source: Journal of Immunological Methods - March 15, 2024 Category: Allergy & Immunology Authors: Nejla Stambouli Khadija Bahrini Chihebeddine Romdhani Aicha Rebai Sana Boughariou Mohamed Zakraoui Bilel Arfaoui Sameh Seyli Yasmine Boukhalfa Riadh Battikh Mohamed Ben Moussa Iheb Labben Mustpha Ferjani Hedi Gharssallah Source Type: research
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study
J Immunol Methods. 2024 Mar 13:113665. doi: 10.1016/j.jim.2024.113665. Online ahead of print.ABSTRACTOn March 13, 2021, Tunisia started a widespread immunization program against SARS-CoV-2 utilizing different vaccinations that had been given emergency approval. Herein, we followed prospectively a cohort of participant who received COVID-19 vaccine (Pfizer BioNTech and Sputnik-Gameleya V). The goal of this follow-up was to define the humoral and cellular immunological profile after immunization by assessing neutralizing antibodies and IFN- γ release. 26 vaccinated health care workers by Pfizer BioNTech (n = 12) and Sputnik...
Source: Journal of Immunological Methods - March 15, 2024 Category: Allergy & Immunology Authors: Nejla Stambouli Khadija Bahrini Chihebeddine Romdhani Aicha Rebai Sana Boughariou Mohamed Zakraoui Bilel Arfaoui Sameh Seyli Yasmine Boukhalfa Riadh Battikh Mohamed Ben Moussa Iheb Labben Mustpha Ferjani Hedi Gharssallah Source Type: research
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study
J Immunol Methods. 2024 Mar 13:113665. doi: 10.1016/j.jim.2024.113665. Online ahead of print.ABSTRACTOn March 13, 2021, Tunisia started a widespread immunization program against SARS-CoV-2 utilizing different vaccinations that had been given emergency approval. Herein, we followed prospectively a cohort of participant who received COVID-19 vaccine (Pfizer BioNTech and Sputnik-Gameleya V). The goal of this follow-up was to define the humoral and cellular immunological profile after immunization by assessing neutralizing antibodies and IFN- γ release. 26 vaccinated health care workers by Pfizer BioNTech (n = 12) and Sputnik...
Source: Journal of Immunological Methods - March 15, 2024 Category: Allergy & Immunology Authors: Nejla Stambouli Khadija Bahrini Chihebeddine Romdhani Aicha Rebai Sana Boughariou Mohamed Zakraoui Bilel Arfaoui Sameh Seyli Yasmine Boukhalfa Riadh Battikh Mohamed Ben Moussa Iheb Labben Mustpha Ferjani Hedi Gharssallah Source Type: research
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study
J Immunol Methods. 2024 Mar 13:113665. doi: 10.1016/j.jim.2024.113665. Online ahead of print.ABSTRACTOn March 13, 2021, Tunisia started a widespread immunization program against SARS-CoV-2 utilizing different vaccinations that had been given emergency approval. Herein, we followed prospectively a cohort of participant who received COVID-19 vaccine (Pfizer BioNTech and Sputnik-Gameleya V). The goal of this follow-up was to define the humoral and cellular immunological profile after immunization by assessing neutralizing antibodies and IFN- γ release. 26 vaccinated health care workers by Pfizer BioNTech (n = 12) and Sputnik...
Source: Journal of Immunological Methods - March 15, 2024 Category: Allergy & Immunology Authors: Nejla Stambouli Khadija Bahrini Chihebeddine Romdhani Aicha Rebai Sana Boughariou Mohamed Zakraoui Bilel Arfaoui Sameh Seyli Yasmine Boukhalfa Riadh Battikh Mohamed Ben Moussa Iheb Labben Mustpha Ferjani Hedi Gharssallah Source Type: research
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study
J Immunol Methods. 2024 Mar 13:113665. doi: 10.1016/j.jim.2024.113665. Online ahead of print.ABSTRACTOn March 13, 2021, Tunisia started a widespread immunization program against SARS-CoV-2 utilizing different vaccinations that had been given emergency approval. Herein, we followed prospectively a cohort of participant who received COVID-19 vaccine (Pfizer BioNTech and Sputnik-Gameleya V). The goal of this follow-up was to define the humoral and cellular immunological profile after immunization by assessing neutralizing antibodies and IFN- γ release. 26 vaccinated health care workers by Pfizer BioNTech (n = 12) and Sputnik...
Source: Journal of Immunological Methods - March 15, 2024 Category: Allergy & Immunology Authors: Nejla Stambouli Khadija Bahrini Chihebeddine Romdhani Aicha Rebai Sana Boughariou Mohamed Zakraoui Bilel Arfaoui Sameh Seyli Yasmine Boukhalfa Riadh Battikh Mohamed Ben Moussa Iheb Labben Mustpha Ferjani Hedi Gharssallah Source Type: research
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study
J Immunol Methods. 2024 Mar 13:113665. doi: 10.1016/j.jim.2024.113665. Online ahead of print.ABSTRACTOn March 13, 2021, Tunisia started a widespread immunization program against SARS-CoV-2 utilizing different vaccinations that had been given emergency approval. Herein, we followed prospectively a cohort of participant who received COVID-19 vaccine (Pfizer BioNTech and Sputnik-Gameleya V). The goal of this follow-up was to define the humoral and cellular immunological profile after immunization by assessing neutralizing antibodies and IFN- γ release. 26 vaccinated health care workers by Pfizer BioNTech (n = 12) and Sputnik...
Source: Journal of Immunological Methods - March 15, 2024 Category: Allergy & Immunology Authors: Nejla Stambouli Khadija Bahrini Chihebeddine Romdhani Aicha Rebai Sana Boughariou Mohamed Zakraoui Bilel Arfaoui Sameh Seyli Yasmine Boukhalfa Riadh Battikh Mohamed Ben Moussa Iheb Labben Mustpha Ferjani Hedi Gharssallah Source Type: research
An optimized non-T cell transfection system based on HEK293FT cells for CD3 ζ phosphorylation and ubiquitination
J Immunol Methods. 2024 Mar 12:113664. doi: 10.1016/j.jim.2024.113664. Online ahead of print.ABSTRACTCD3ζ is part of the T cell receptor (TCR)/CD3 complex that plays a critical role in antigen recognition and subsequent T cell activation. Understanding the mechanisms that regulate CD3ζ can provide new insights into the T cell-mediated immune responses. However, it is challenging to deliver exogenous genes into T cells for functional and mechanistic analyses. To this end, we established a non-T cell transfection system based on HEK293FT cells to screen for candidate regulatory proteins. The transfection was optimized usin...
Source: Journal of Immunological Methods - March 14, 2024 Category: Allergy & Immunology Authors: Jiaqi Zheng Yuchuan Zhang Yiting Cai Wei Han Wei Chen Source Type: research
Selection of positive controls and their impact on anti-drug antibody assay performance
J Immunol Methods. 2024 Mar 11;528:113657. doi: 10.1016/j.jim.2024.113657. Online ahead of print.ABSTRACTDevelopment of assays to reliably identify and characterize anti-drug antibodies (ADAs) depends on positive control anti-idiotype (anti-id) reagents, which are used to demonstrate that the standards recommended by regulatory authorities are met. This work employs a set of therapeutic antibodies under clinical development and their corresponding anti-ids to investigate how different positive control reagent properties impact ADA assay development. Positive controls exhibited different response profiles and apparent assay...
Source: Journal of Immunological Methods - March 13, 2024 Category: Allergy & Immunology Authors: Joshua A Weiner Harini Natarajan Calum J McIntosh Eun Sung Yang Misook Choe Cassidy L Papia Katherine S Axelrod Gabriela Kovacikova Amarendra Pegu Margaret E Ackerman Source Type: research
Selection of positive controls and their impact on anti-drug antibody assay performance
J Immunol Methods. 2024 Mar 11:113657. doi: 10.1016/j.jim.2024.113657. Online ahead of print.ABSTRACTDevelopment of assays to reliably identify and characterize anti-drug antibodies (ADAs) depends on positive control anti-idiotype (anti-id) reagents, which are used to demonstrate that the standards recommended by regulatory authorities are met. This work employs a set of therapeutic antibodies under clinical development and their corresponding anti-ids to investigate how different positive control reagent properties impact ADA assay development. Positive controls exhibited different response profiles and apparent assay ana...
Source: Journal of Immunological Methods - March 13, 2024 Category: Allergy & Immunology Authors: Joshua A Weiner Harini Natarajan Calum J McIntosh Eun Sung Yang Misook Choe Cassidy L Papia Katherine S Axelrod Gabriela Kovacikova Amarendra Pegu Margaret E Ackerman Source Type: research
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera
J Immunol Methods. 2024 Mar 6:113652. doi: 10.1016/j.jim.2024.113652. Online ahead of print.ABSTRACTStreptococcus pyogenes, commonly referred to as Group A Streptococcus (Strep A), causes a spectrum of diseases, with the potential to progress into life-threatening illnesses and autoimmune complications. The escalating threat of antimicrobial resistance, stemming from the prevalent reliance on antibiotic therapies to manage Strep A infections, underscores the critical need for the development of disease control strategies centred around vaccination. Phagocytes play a critical role in controlling Strep A infections, and phag...
Source: Journal of Immunological Methods - March 8, 2024 Category: Allergy & Immunology Authors: Elena Boero Martina Carducci Alexander J Keeley Francesco Berlanda Scorza Miren Iturriza Danilo Gomes Moriel Omar Rossi Source Type: research
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera
J Immunol Methods. 2024 Mar 6:113652. doi: 10.1016/j.jim.2024.113652. Online ahead of print.ABSTRACTStreptococcus pyogenes, commonly referred to as Group A Streptococcus (Strep A), causes a spectrum of diseases, with the potential to progress into life-threatening illnesses and autoimmune complications. The escalating threat of antimicrobial resistance, stemming from the prevalent reliance on antibiotic therapies to manage Strep A infections, underscores the critical need for the development of disease control strategies centred around vaccination. Phagocytes play a critical role in controlling Strep A infections, and phag...
Source: Journal of Immunological Methods - March 8, 2024 Category: Allergy & Immunology Authors: Elena Boero Martina Carducci Alexander J Keeley Francesco Berlanda Scorza Miren Iturriza Danilo Gomes Moriel Omar Rossi Source Type: research
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera
J Immunol Methods. 2024 Mar 6:113652. doi: 10.1016/j.jim.2024.113652. Online ahead of print.ABSTRACTStreptococcus pyogenes, commonly referred to as Group A Streptococcus (Strep A), causes a spectrum of diseases, with the potential to progress into life-threatening illnesses and autoimmune complications. The escalating threat of antimicrobial resistance, stemming from the prevalent reliance on antibiotic therapies to manage Strep A infections, underscores the critical need for the development of disease control strategies centred around vaccination. Phagocytes play a critical role in controlling Strep A infections, and phag...
Source: Journal of Immunological Methods - March 8, 2024 Category: Allergy & Immunology Authors: Elena Boero Martina Carducci Alexander J Keeley Francesco Berlanda Scorza Miren Iturriza Danilo Gomes Moriel Omar Rossi Source Type: research
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera
J Immunol Methods. 2024 Mar 6:113652. doi: 10.1016/j.jim.2024.113652. Online ahead of print.ABSTRACTStreptococcus pyogenes, commonly referred to as Group A Streptococcus (Strep A), causes a spectrum of diseases, with the potential to progress into life-threatening illnesses and autoimmune complications. The escalating threat of antimicrobial resistance, stemming from the prevalent reliance on antibiotic therapies to manage Strep A infections, underscores the critical need for the development of disease control strategies centred around vaccination. Phagocytes play a critical role in controlling Strep A infections, and phag...
Source: Journal of Immunological Methods - March 8, 2024 Category: Allergy & Immunology Authors: Elena Boero Martina Carducci Alexander J Keeley Francesco Berlanda Scorza Miren Iturriza Danilo Gomes Moriel Omar Rossi Source Type: research
Performance of Softcup ® menstrual cup and vulvovaginal swab samples for detection and quantification of genital cytokines
J Immunol Methods. 2024 Mar 5;528:113656. doi: 10.1016/j.jim.2024.113656. Online ahead of print.ABSTRACTCytokines are important mediators of immunity in the female genital tract, and their levels may be associated with various reproductive health outcomes. However, the measurement of cytokines and chemokines in vaginal fluid samples may be influenced by a variety of factors, each with the potential to affect the sensitivity and accuracy of the assay, including the interpretation and comparison of data. We measured and compared cytokine milieu in samples collected via Softcup® menstrual cup versus vulvovaginal swabs. One h...
Source: Journal of Immunological Methods - March 6, 2024 Category: Allergy & Immunology Authors: Nashlin Pillay Gugulethu Favourate Mzobe Marothi Letsoalo Asavela Olona Kama Andile Mtshali Stanley Nzuzo Magini Nikkishia Singh Vani Govender Natasha Samsunder Megeshinee Naidoo Dhayendre Moodley Cheryl Baxter Derseree Archary Sinaye Ngcapu Source Type: research
Development and basic performance verification of a rapid homogeneous bioassay for agonistic antibodies against the thyroid-stimulating hormone receptor
J Immunol Methods. 2024 Mar 4:113655. doi: 10.1016/j.jim.2024.113655. Online ahead of print.ABSTRACTGraves' disease is a type of autoimmune hyperthyroidism caused by thyroid-stimulating antibodies (TSAb).1 The combination of a porcine thyroid cell bioassay and cyclic adenosine monophosphate (cAMP) immunoassay (TSAb-enzyme immunoassay; EIA) is a clinically approved TSAb measurement method. Due to the requirement of multiple procedures and a long assay time of 6 h in the TSAb-EIA, a simplified and rapid assay is desired. Herein, we developed a rapid homogeneous TSAb bioassay (rapid-TSAb assay) using the human embryonic kidne...
Source: Journal of Immunological Methods - March 6, 2024 Category: Allergy & Immunology Authors: Motoki Hoshina Shiomi Ojima Atsushi Kawasaki Kosuke Doi Satoshi Ohta Asuka Inoue Hiroshi Murayama Source Type: research
