Impact of bridge heterology on functional parameters of ELISA for 17 α-methyltestosterone
In this study, we have developed bridge heterologous ELISA for the detection of 17 α Methyl testosterone by incorporating aromatic spacers between 17α Methyl testosterone-3-Carboxymethyloxime and Horseradish peroxidase label through N-hydroxysuccinimide mediated carbodiimide reaction method. The immunogen 17α Methyl testosterone-3-Carboxymethyloxime-Bovine serum albumin used to generate the antibody was also prepared by the N-hydroxysuccinimide mediated carbodiimide reaction without using any spacer. We have studied the impact of bridge/aromatic spacers on functional parameters i.e. sensitivity, affinity and ED50 of the...
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Divya Verma T G Shrivastav Sonu Chand Thakur Source Type: research
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay
CONCLUSIONS: As an assessment tool, we found that our AlphaLISA had good consistency with an ELISA and had several other advantages, including requiring a shorter processing time and detecting a wider range of serum HNL concentrations. Monitoring serum HNL levels of patients admitted to the ICU might be useful in distinguishing sepsis patients who have septic shock from other sepsis patients, indicating its value in the prediction of sepsis patient prognosis.PMID:37774777 | DOI:10.1016/j.jim.2023.113570 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - September 29, 2023 Category: Allergy & Immunology Authors: Huijun Zong Xueyi Shang Xin Wang Ting Chen Ye Wang Yuhao Ren Yongqiang Jiang Yan Li Qingyu Lv Peng Liu Source Type: research
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies
This study aimed to standardize, validate, and compare it with the reference method in 6-well plates (PRNT). Once the execution protocol was standardized, precision, accuracy, selectivity, and robustness were evaluated to validate the μFRN-HRP. In addition, 200 sera of vaccinees were processed by the μFRN-HRP and by the micro-PRNT to compare with the reference test, estimating agreement by Intraclass Correlation Coefficient (ICC). The standardization and validation of the μFRN-HRP was carried out successfully. Weak to moderate agreement was observed between μFRN-HRP and PRNT for titers in reciprocal dilution, while the...
Source: Journal of Immunological Methods - September 25, 2023 Category: Allergy & Immunology Authors: Marisol Sim ões Stephanie Almeida da Silva Kelly Ara újo Lúcio Renan de Oliveira Vieira Waleska Dias Schwarcz Sheila Maria Barbosa de Lima Luiz Antonio Bastos Camacho Source Type: research
A systematic examination of anti-drug antibody titer estimation: Applied recommendations
J Immunol Methods. 2023 Sep 23;522:113569. doi: 10.1016/j.jim.2023.113569. Online ahead of print.ABSTRACTBiologic drugs (therapeutic proteins or peptides) have become one of the most important therapeutic modalities over the past few decades. Drug-induced immunogenicity is a significant concern as it may affect safety, tolerability, and efficacy. With more sensitive and drug-tolerant screening assays in use today, reliable estimation of anti-drug-antibody (ADA) titer has become more important for understanding clinically relevant ADA levels. Titer is commonly defined as the dilution factor resulting in an assay signal equa...
Source: Journal of Immunological Methods - September 25, 2023 Category: Allergy & Immunology Authors: Lin Zhang Michael E Hodsdon Thomas Pottanat Sihe Wang Robert John Konrad Nicholas Seta Richard E Higgs Source Type: research
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies
This study aimed to standardize, validate, and compare it with the reference method in 6-well plates (PRNT). Once the execution protocol was standardized, precision, accuracy, selectivity, and robustness were evaluated to validate the μFRN-HRP. In addition, 200 sera of vaccinees were processed by the μFRN-HRP and by the micro-PRNT to compare with the reference test, estimating agreement by Intraclass Correlation Coefficient (ICC). The standardization and validation of the μFRN-HRP was carried out successfully. Weak to moderate agreement was observed between μFRN-HRP and PRNT for titers in reciprocal dilution, while the...
Source: Journal of Immunological Methods - September 25, 2023 Category: Allergy & Immunology Authors: Marisol Sim ões Stephanie Almeida da Silva Kelly Ara újo Lúcio Renan de Oliveira Vieira Waleska Dias Schwarcz Sheila Maria Barbosa de Lima Luiz Antonio Bastos Camacho Source Type: research
A systematic examination of anti-drug antibody titer estimation: Applied recommendations
J Immunol Methods. 2023 Sep 23:113569. doi: 10.1016/j.jim.2023.113569. Online ahead of print.ABSTRACTBiologic drugs (therapeutic proteins or peptides) have become one of the most important therapeutic modalities over the past few decades. Drug-induced immunogenicity is a significant concern as it may affect safety, tolerability, and efficacy. With more sensitive and drug-tolerant screening assays in use today, reliable estimation of anti-drug-antibody (ADA) titer has become more important for understanding clinically relevant ADA levels. Titer is commonly defined as the dilution factor resulting in an assay signal equal to...
Source: Journal of Immunological Methods - September 25, 2023 Category: Allergy & Immunology Authors: Lin Zhang Michael E Hodsdon Thomas Pottanat Sihe Wang Robert John Konrad Nicholas Seta Richard E Higgs Source Type: research
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies
This study aimed to standardize, validate, and compare it with the reference method in 6-well plates (PRNT). Once the execution protocol was standardized, precision, accuracy, selectivity, and robustness were evaluated to validate the μFRN-HRP. In addition, 200 sera of vaccinees were processed by the μFRN-HRP and by the micro-PRNT to compare with the reference test, estimating agreement by Intraclass Correlation Coefficient (ICC). The standardization and validation of the μFRN-HRP was carried out successfully. Weak to moderate agreement was observed between μFRN-HRP and PRNT for titers in reciprocal dilution, while the...
Source: Journal of Immunological Methods - September 25, 2023 Category: Allergy & Immunology Authors: Marisol Sim ões Stephanie Almeida da Silva Kelly Ara újo Lúcio Renan de Oliveira Vieira Waleska Dias Schwarcz Sheila Maria Barbosa de Lima Luiz Antonio Bastos Camacho Source Type: research
A systematic examination of anti-drug antibody titer estimation: Applied recommendations
J Immunol Methods. 2023 Sep 23:113569. doi: 10.1016/j.jim.2023.113569. Online ahead of print.ABSTRACTBiologic drugs (therapeutic proteins or peptides) have become one of the most important therapeutic modalities over the past few decades. Drug-induced immunogenicity is a significant concern as it may affect safety, tolerability, and efficacy. With more sensitive and drug-tolerant screening assays in use today, reliable estimation of anti-drug-antibody (ADA) titer has become more important for understanding clinically relevant ADA levels. Titer is commonly defined as the dilution factor resulting in an assay signal equal to...
Source: Journal of Immunological Methods - September 25, 2023 Category: Allergy & Immunology Authors: Lin Zhang Michael E Hodsdon Thomas Pottanat Sihe Wang Robert John Konrad Nicholas Seta Richard E Higgs Source Type: research
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies
This study aimed to standardize, validate, and compare it with the reference method in 6-well plates (PRNT). Once the execution protocol was standardized, precision, accuracy, selectivity, and robustness were evaluated to validate the μFRN-HRP. In addition, 200 sera of vaccinees were processed by the μFRN-HRP and by the micro-PRNT to compare with the reference test, estimating agreement by Intraclass Correlation Coefficient (ICC). The standardization and validation of the μFRN-HRP was carried out successfully. Weak to moderate agreement was observed between μFRN-HRP and PRNT for titers in reciprocal dilution, while the...
Source: Journal of Immunological Methods - September 25, 2023 Category: Allergy & Immunology Authors: Marisol Sim ões Stephanie Almeida da Silva Kelly Ara újo Lúcio Renan de Oliveira Vieira Waleska Dias Schwarcz Sheila Maria Barbosa de Lima Luiz Antonio Bastos Camacho Source Type: research
A systematic examination of anti-drug antibody titer estimation: Applied recommendations
J Immunol Methods. 2023 Sep 23:113569. doi: 10.1016/j.jim.2023.113569. Online ahead of print.ABSTRACTBiologic drugs (therapeutic proteins or peptides) have become one of the most important therapeutic modalities over the past few decades. Drug-induced immunogenicity is a significant concern as it may affect safety, tolerability, and efficacy. With more sensitive and drug-tolerant screening assays in use today, reliable estimation of anti-drug-antibody (ADA) titer has become more important for understanding clinically relevant ADA levels. Titer is commonly defined as the dilution factor resulting in an assay signal equal to...
Source: Journal of Immunological Methods - September 25, 2023 Category: Allergy & Immunology Authors: Lin Zhang Michael E Hodsdon Thomas Pottanat Sihe Wang Robert John Konrad Nicholas Seta Richard E Higgs Source Type: research
Validation of the use of dried blood spots in a chikungunya virus IgG serological assay
J Immunol Methods. 2023 Sep 22:113571. doi: 10.1016/j.jim.2023.113571. Online ahead of print.ABSTRACTDried blood spot (DBS) sampling is a simple, fast, and minimally invasive blood collection method that is particularly useful for diagnostic or epidemiological studies in hard-to-reach populations. Nevertheless, the use of DBS in assays that have been optimized with gold-standard samples (serum or plasma) must be optimized to yield reliable results. Here, we describe the validation of DBS in a commercial assay to measure IgG against chikungunya virus (CHIKV IgG ELISA; Euroimmun, Lübeck, Germany). During a health survey of ...
Source: Journal of Immunological Methods - September 24, 2023 Category: Allergy & Immunology Authors: Tereza Magalhaes Moyra M Portilho Patricia S S Moreira Milena L Marinho Wiler P Dias Nat ália M Gonçalves Osiyall ê A S Rodrigues Jane Montes Leila Reis Dilma F Jesus Tarc ísio O Silva Lua S Dultra Joilda S Nery Guilherme S Ribeiro Source Type: research
Validation of the use of dried blood spots in a chikungunya virus IgG serological assay
J Immunol Methods. 2023 Sep 22:113571. doi: 10.1016/j.jim.2023.113571. Online ahead of print.ABSTRACTDried blood spot (DBS) sampling is a simple, fast, and minimally invasive blood collection method that is particularly useful for diagnostic or epidemiological studies in hard-to-reach populations. Nevertheless, the use of DBS in assays that have been optimized with gold-standard samples (serum or plasma) must be optimized to yield reliable results. Here, we describe the validation of DBS in a commercial assay to measure IgG against chikungunya virus (CHIKV IgG ELISA; Euroimmun, Lübeck, Germany). During a health survey of ...
Source: Journal of Immunological Methods - September 24, 2023 Category: Allergy & Immunology Authors: Tereza Magalhaes Moyra M Portilho Patricia S S Moreira Milena L Marinho Wiler P Dias Nat ália M Gonçalves Osiyall ê A S Rodrigues Jane Montes Leila Reis Dilma F Jesus Tarc ísio O Silva Lua S Dultra Joilda S Nery Guilherme S Ribeiro Source Type: research
Obtaining a high titer of polyclonal antibodies from rats to the SARS-CoV-2 nucleocapsid protein and its N- and C-terminal domains for diagnostic test development
J Immunol Methods. 2023 Sep 11:113558. doi: 10.1016/j.jim.2023.113558. Online ahead of print.ABSTRACTSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is an enveloped, plus-stranded RNA virus responsible for the Coronavirus Disease 2019 (COVID-19). Patients infected with COVID-19 may be asymptomatic or have symptoms ranging from mild manifestations to severe cases of the disease that could lead to death. The SARS-CoV-2 genome encodes 4 structural proteins, including the Spike protein (S), the Nucleocapsid protein (N), membrane protein (M) and, the envelope (E). The N protein forms a major component of the ribonu...
Source: Journal of Immunological Methods - September 13, 2023 Category: Allergy & Immunology Authors: Michelle Teixeira de Almeida Ana Paula Barbosa Camila Gasque Bomfim Aline Biazola Visnardi Tania Churasacari Vinces Alexandre Ceroni Edison Luiz Durigon Cristiane Rodrigues Guzzo Source Type: research
Obtaining a high titer of polyclonal antibodies from rats to the SARS-CoV-2 nucleocapsid protein and its N- and C-terminal domains for diagnostic test development
J Immunol Methods. 2023 Sep 11:113558. doi: 10.1016/j.jim.2023.113558. Online ahead of print.ABSTRACTSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is an enveloped, plus-stranded RNA virus responsible for the Coronavirus Disease 2019 (COVID-19). Patients infected with COVID-19 may be asymptomatic or have symptoms ranging from mild manifestations to severe cases of the disease that could lead to death. The SARS-CoV-2 genome encodes 4 structural proteins, including the Spike protein (S), the Nucleocapsid protein (N), membrane protein (M) and, the envelope (E). The N protein forms a major component of the ribonu...
Source: Journal of Immunological Methods - September 13, 2023 Category: Allergy & Immunology Authors: Michelle Teixeira de Almeida Ana Paula Barbosa Camila Gasque Bomfim Aline Biazola Visnardi Tania Churasacari Vinces Alexandre Ceroni Edison Luiz Durigon Cristiane Rodrigues Guzzo Source Type: research
Obtaining a high titer of polyclonal antibodies from rats to the SARS-CoV-2 nucleocapsid protein and its N- and C-terminal domains for diagnostic test development
J Immunol Methods. 2023 Sep 11:113558. doi: 10.1016/j.jim.2023.113558. Online ahead of print.ABSTRACTSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is an enveloped, plus-stranded RNA virus responsible for the Coronavirus Disease 2019 (COVID-19). Patients infected with COVID-19 may be asymptomatic or have symptoms ranging from mild manifestations to severe cases of the disease that could lead to death. The SARS-CoV-2 genome encodes 4 structural proteins, including the Spike protein (S), the Nucleocapsid protein (N), membrane protein (M) and, the envelope (E). The N protein forms a major component of the ribonu...
Source: Journal of Immunological Methods - September 13, 2023 Category: Allergy & Immunology Authors: Michelle Teixeira de Almeida Ana Paula Barbosa Camila Gasque Bomfim Aline Biazola Visnardi Tania Churasacari Vinces Alexandre Ceroni Edison Luiz Durigon Cristiane Rodrigues Guzzo Source Type: research
