Transcriptome analysis reveals immune function-related mRNA expression in donkey mammary glands during four developmental stages
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Dec 7;49:101169. doi: 10.1016/j.cbd.2023.101169. Online ahead of print.ABSTRACTThe low susceptibility to mastitis of female donkey (jenny) mammary glands and the strong immune properties of donkey milk are acknowledged, but little is known about the genes involved in mammary gland immunity in jennies. Herein, we used RNA-sequencing and bioinformatics analyses to explore jenny mammary gland transcriptomes and detect potential functional differentially expressed (DE) mRNAs related to immunity during four specific developmental stages: foetal (F), pubertal (P), adult parou...
Source: Genomics Proteomics ... - December 14, 2023 Category: Genetics & Stem Cells Authors: Xinyue Wang Yaqi Fei Yang Shao Qingchao Liao Qingze Meng Ran Chen Liang Deng Source Type: research
Transcriptome analysis reveals immune function-related mRNA expression in donkey mammary glands during four developmental stages
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Dec 7;49:101169. doi: 10.1016/j.cbd.2023.101169. Online ahead of print.ABSTRACTThe low susceptibility to mastitis of female donkey (jenny) mammary glands and the strong immune properties of donkey milk are acknowledged, but little is known about the genes involved in mammary gland immunity in jennies. Herein, we used RNA-sequencing and bioinformatics analyses to explore jenny mammary gland transcriptomes and detect potential functional differentially expressed (DE) mRNAs related to immunity during four specific developmental stages: foetal (F), pubertal (P), adult parou...
Source: Genomics Proteomics ... - December 14, 2023 Category: Genetics & Stem Cells Authors: Xinyue Wang Yaqi Fei Yang Shao Qingchao Liao Qingze Meng Ran Chen Liang Deng Source Type: research
Ursa: A Comprehensive Multi-Omics Toolbox for High-Throughput Single-Cell Analysis
Mol Biol Evol. 2023 Dec 13:msad267. doi: 10.1093/molbev/msad267. Online ahead of print.ABSTRACTThe burgeoning amount of single-cell data has been accompanied by revolutionary changes to computational methods to map, quantify and analyze the outputs of these cutting-edge technologies. Many are still unable to reap the benefits of these advancements due to the lack of bioinformatics expertise. To address this issue, we present Ursa, an automated single-cell multi-omics R package containing six automated single-cell omics and spatial transcriptomics workflows. Ursa allows scientists to carry out post-quantification single or ...
Source: Genomics Proteomics ... - December 13, 2023 Category: Genetics & Stem Cells Authors: Lu Pan Tian Mou Yue Huang Weifeng Hong Min Yu Xuexin Li Source Type: research
Toxic effects of lead on plants: integrating multi-omics with bioinformatics to develop Pb-tolerant crops
Planta. 2023 Dec 12;259(1):18. doi: 10.1007/s00425-023-04296-9.ABSTRACTLead disrupts plant metabolic homeostasis and key structural elements. Utilizing modern biotechnology tools, it's feasible to develop Pb-tolerant varieties by discovering biological players regulating plant metabolic pathways under stress. Lead (Pb) has been used for a variety of purposes since antiquity despite its toxic nature. After arsenic, lead is the most hazardous heavy metal without any known beneficial role in the biological system. It is a crucial inorganic pollutant that affects plant biochemical and morpho-physiological attributes. Lead toxi...
Source: Genomics Proteomics ... - December 12, 2023 Category: Genetics & Stem Cells Authors: Muhammad Zahaib Ilyas Kyu Jin Sa Muhammad Waqas Ali Ju Kyong Lee Source Type: research
Toxic effects of lead on plants: integrating multi-omics with bioinformatics to develop Pb-tolerant crops
Planta. 2023 Dec 12;259(1):18. doi: 10.1007/s00425-023-04296-9.ABSTRACTLead disrupts plant metabolic homeostasis and key structural elements. Utilizing modern biotechnology tools, it's feasible to develop Pb-tolerant varieties by discovering biological players regulating plant metabolic pathways under stress. Lead (Pb) has been used for a variety of purposes since antiquity despite its toxic nature. After arsenic, lead is the most hazardous heavy metal without any known beneficial role in the biological system. It is a crucial inorganic pollutant that affects plant biochemical and morpho-physiological attributes. Lead toxi...
Source: Genomics Proteomics ... - December 12, 2023 Category: Genetics & Stem Cells Authors: Muhammad Zahaib Ilyas Kyu Jin Sa Muhammad Waqas Ali Ju Kyong Lee Source Type: research
Simulation-predicted and -explained inheritance model of pathogenicity confirmed by transgenic mice models
In this study, using molecular dynamics (MD) simulations, we showed that detached inner-wall N-terminal "plugs" aggregated to reduce the channel ion flow in a highly prevalent V37I variant in humans. To examine the predictive ability of the computational platform, an artificial mutant, V37M, of which the effect was previously unknown in hearing loss, was created. Microsecond simulations showed that homo-hexameric V37M Cx26 hemichannels had an abnormal affinity between the inner edge and N-termini to block the narrower side of the cone-shaped Cx26, while the most stable hetero-hexameric channels did not. From the perspectiv...
Source: Genomics Proteomics ... - December 11, 2023 Category: Genetics & Stem Cells Authors: Cheng-Yu Tsai Ying-Chang Lu Yen-Hui Chan Navaneethan Radhakrishnan Yuan-Yu Chang Shu-Wha Lin Tien-Chen Liu Chuan-Jen Hsu Pei-Lung Chen Lee-Wei Yang Chen-Chi Wu Source Type: research
Screening for Circulating Inflammatory Proteins Does Not Reveal Plasma Biomarkers of Constant Tinnitus
DISCUSSION: Our results did not identify significant associations of the selected inflammatory proteins with constant tinnitus. Future studies examining longitudinal relations among those with more severe tinnitus and using more recent expanded proteomics platforms and sampling of cerebrospinal fluid could increase the likelihood of identifying relevant molecular biomarkers.PMID:38079022 | DOI:10.1007/s10162-023-00920-3 (Source: Genomics Proteomics ...)
Source: Genomics Proteomics ... - December 11, 2023 Category: Genetics & Stem Cells Authors: Christopher R Cederroth Mun-Gwan Hong Maxim B Freydin Niklas K Edvall Natalia Trpchevska Carlotta Jarach Winfried Schlee Jochen M Schwenk Jose-Antonio Lopez-Escamez Silvano Gallus Barbara Canlon Jan Bulla Frances M K Williams Source Type: research
Simulation-predicted and -explained inheritance model of pathogenicity confirmed by transgenic mice models
In this study, using molecular dynamics (MD) simulations, we showed that detached inner-wall N-terminal "plugs" aggregated to reduce the channel ion flow in a highly prevalent V37I variant in humans. To examine the predictive ability of the computational platform, an artificial mutant, V37M, of which the effect was previously unknown in hearing loss, was created. Microsecond simulations showed that homo-hexameric V37M Cx26 hemichannels had an abnormal affinity between the inner edge and N-termini to block the narrower side of the cone-shaped Cx26, while the most stable hetero-hexameric channels did not. From the perspectiv...
Source: Genomics Proteomics ... - December 11, 2023 Category: Genetics & Stem Cells Authors: Cheng-Yu Tsai Ying-Chang Lu Yen-Hui Chan Navaneethan Radhakrishnan Yuan-Yu Chang Shu-Wha Lin Tien-Chen Liu Chuan-Jen Hsu Pei-Lung Chen Lee-Wei Yang Chen-Chi Wu Source Type: research
Screening for Circulating Inflammatory Proteins Does Not Reveal Plasma Biomarkers of Constant Tinnitus
DISCUSSION: Our results did not identify significant associations of the selected inflammatory proteins with constant tinnitus. Future studies examining longitudinal relations among those with more severe tinnitus and using more recent expanded proteomics platforms and sampling of cerebrospinal fluid could increase the likelihood of identifying relevant molecular biomarkers.PMID:38079022 | DOI:10.1007/s10162-023-00920-3 (Source: Genomics Proteomics ...)
Source: Genomics Proteomics ... - December 11, 2023 Category: Genetics & Stem Cells Authors: Christopher R Cederroth Mun-Gwan Hong Maxim B Freydin Niklas K Edvall Natalia Trpchevska Carlotta Jarach Winfried Schlee Jochen M Schwenk Jose-Antonio Lopez-Escamez Silvano Gallus Barbara Canlon Jan Bulla Frances M K Williams Source Type: research
ROS-induced ribosome impairment underlies ZAK α-mediated metabolic decline in obesity and aging
Science. 2023 Dec 8;382(6675):eadf3208. doi: 10.1126/science.adf3208. Epub 2023 Dec 8.ABSTRACTThe ribotoxic stress response (RSR) is a signaling pathway in which the p38- and c-Jun N-terminal kinase (JNK)-activating mitogen-activated protein kinase kinase kinase (MAP3K) ZAKα senses stalling and/or collision of ribosomes. Here, we show that reactive oxygen species (ROS)-generating agents trigger ribosomal impairment and ZAKα activation. Conversely, zebrafish larvae deficient for ZAKα are protected from ROS-induced pathology. Livers of mice fed a ROS-generating diet exhibit ZAKα-activating changes in ribosomal elongation...
Source: Genomics Proteomics ... - December 7, 2023 Category: Genetics & Stem Cells Authors: Goda Snieckute Laura Ryder Anna Constance Vind Zhenzhen Wu Frederic Schr øder Arendrup Mark Stoneley S ébastien Chamois Ana Martinez-Val Marion Leleu Ren é Dreos Alexander Russell David Michael Gay Aitana Victoria Genzor Beatrice So-Yun Choi Astrid Lin Source Type: research
Genomic analysis reveals novel allergens of Blomia tropicalis
Allergol Int. 2023 Dec 6:S1323-8930(23)00116-8. doi: 10.1016/j.alit.2023.11.004. Online ahead of print.NO ABSTRACTPMID:38061933 | DOI:10.1016/j.alit.2023.11.004 (Source: Genomics Proteomics ...)
Source: Genomics Proteomics ... - December 7, 2023 Category: Genetics & Stem Cells Authors: Qing Xiong Xiaoyu Liu Angel Tsz-Yau Wan Nat Malainual Xiaojun Xiao Hui Cao Man-Fung Tang Judy Kin-Wing Ng Soo-Kyung Shin Yang Yie Sio Mingqiang Wang Baoqing Sun Ting-Fan Leung Fook Tim Chew Anchalee Tungtrongchitr Stephen Kwok-Wing Tsui Source Type: research
ROS-induced ribosome impairment underlies ZAK α-mediated metabolic decline in obesity and aging
Science. 2023 Dec 8;382(6675):eadf3208. doi: 10.1126/science.adf3208. Epub 2023 Dec 8.ABSTRACTThe ribotoxic stress response (RSR) is a signaling pathway in which the p38- and c-Jun N-terminal kinase (JNK)-activating mitogen-activated protein kinase kinase kinase (MAP3K) ZAKα senses stalling and/or collision of ribosomes. Here, we show that reactive oxygen species (ROS)-generating agents trigger ribosomal impairment and ZAKα activation. Conversely, zebrafish larvae deficient for ZAKα are protected from ROS-induced pathology. Livers of mice fed a ROS-generating diet exhibit ZAKα-activating changes in ribosomal elongation...
Source: Genomics Proteomics ... - December 7, 2023 Category: Genetics & Stem Cells Authors: Goda Snieckute Laura Ryder Anna Constance Vind Zhenzhen Wu Frederic Schr øder Arendrup Mark Stoneley S ébastien Chamois Ana Martinez-Val Marion Leleu Ren é Dreos Alexander Russell David Michael Gay Aitana Victoria Genzor Beatrice So-Yun Choi Astrid Lin Source Type: research
Genomic analysis reveals novel allergens of Blomia tropicalis
Allergol Int. 2023 Dec 6:S1323-8930(23)00116-8. doi: 10.1016/j.alit.2023.11.004. Online ahead of print.NO ABSTRACTPMID:38061933 | DOI:10.1016/j.alit.2023.11.004 (Source: Genomics Proteomics ...)
Source: Genomics Proteomics ... - December 7, 2023 Category: Genetics & Stem Cells Authors: Qing Xiong Xiaoyu Liu Angel Tsz-Yau Wan Nat Malainual Xiaojun Xiao Hui Cao Man-Fung Tang Judy Kin-Wing Ng Soo-Kyung Shin Yang Yie Sio Mingqiang Wang Baoqing Sun Ting-Fan Leung Fook Tim Chew Anchalee Tungtrongchitr Stephen Kwok-Wing Tsui Source Type: research
ROS-induced ribosome impairment underlies ZAK α-mediated metabolic decline in obesity and aging
Science. 2023 Dec 8;382(6675):eadf3208. doi: 10.1126/science.adf3208. Epub 2023 Dec 8.ABSTRACTThe ribotoxic stress response (RSR) is a signaling pathway in which the p38- and c-Jun N-terminal kinase (JNK)-activating mitogen-activated protein kinase kinase kinase (MAP3K) ZAKα senses stalling and/or collision of ribosomes. Here, we show that reactive oxygen species (ROS)-generating agents trigger ribosomal impairment and ZAKα activation. Conversely, zebrafish larvae deficient for ZAKα are protected from ROS-induced pathology. Livers of mice fed a ROS-generating diet exhibit ZAKα-activating changes in ribosomal elongation...
Source: Genomics Proteomics ... - December 7, 2023 Category: Genetics & Stem Cells Authors: Goda Snieckute Laura Ryder Anna Constance Vind Zhenzhen Wu Frederic Schr øder Arendrup Mark Stoneley S ébastien Chamois Ana Martinez-Val Marion Leleu Ren é Dreos Alexander Russell David Michael Gay Aitana Victoria Genzor Beatrice So-Yun Choi Astrid Lin Source Type: research
Genomic analysis reveals novel allergens of Blomia tropicalis
Allergol Int. 2023 Dec 6:S1323-8930(23)00116-8. doi: 10.1016/j.alit.2023.11.004. Online ahead of print.NO ABSTRACTPMID:38061933 | DOI:10.1016/j.alit.2023.11.004 (Source: Genomics Proteomics ...)
Source: Genomics Proteomics ... - December 7, 2023 Category: Genetics & Stem Cells Authors: Qing Xiong Xiaoyu Liu Angel Tsz-Yau Wan Nat Malainual Xiaojun Xiao Hui Cao Man-Fung Tang Judy Kin-Wing Ng Soo-Kyung Shin Yang Yie Sio Mingqiang Wang Baoqing Sun Ting-Fan Leung Fook Tim Chew Anchalee Tungtrongchitr Stephen Kwok-Wing Tsui Source Type: research
