BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
Vibration spectra of DNA and RNA segments
Eur Biophys J. 2024 Jan 24. doi: 10.1007/s00249-023-01699-0. Online ahead of print.ABSTRACTThe dispersion curves and density of states are used to analyze the vibrational characteristics of DNA and RNA segments. This is done using a harmonic Hamiltonian and the Green's function technique. Two configurations of DNA and RNA, finite and cyclic, have been investigated and compared to their infinite counterparts. For the DNA molecule, three models, including a fishbone model, a ldder model, and a fishbone ladder model, have been employed, while the RNA molecule has been represented using a half fishbone model. To enhance the re...
Source: European Biophysics Journal : EBJ - January 24, 2024 Category: Physics Authors: Samira Jalilvand Hamze Mousavi Source Type: research
Real-time tilting and twisting motions of ligand-bound states of α7 nicotinic acetylcholine receptor
Eur Biophys J. 2024 Feb;53(1-2):15-25. doi: 10.1007/s00249-023-01693-6. Epub 2024 Jan 17.ABSTRACTThe α7 nicotinic acetylcholine receptor is a member of the nicotinic acetylcholine receptor family and is composed of five α7 subunits arranged symmetrically around a central pore. It is localized in the central nervous system and immune cells and could be a target for treating Alzheimer's disease and schizophrenia. Acetylcholine is a ligand that opens the channel, although prolonged application rapidly decreases the response. Ivermectin was reported as one of the positive allosteric modulators, since the binding of Ivermecti...
Source: European Biophysics Journal : EBJ - January 17, 2024 Category: Physics Authors: Yue Yang Tatsuya Arai Daisuke Sasaki Masahiro Kuramochi Hidetoshi Inagaki Sumiko Ohashi Hiroshi Sekiguchi Kazuhiro Mio Tai Kubo Yuji C Sasaki Source Type: research
Quenching of G4-DNA intrinsic fluorescence by ligands
Eur Biophys J. 2024 Feb;53(1-2):47-56. doi: 10.1007/s00249-023-01696-3. Epub 2024 Jan 13.ABSTRACTG-quadruplex (G4) structures formed by the guanine-rich DNA regions exhibit several distinctive optical properties, including UV absorption and circular dichroism spectra. Some G4 DNA possess intrinsic UV fluorescence whose origin is not completely clear to date. In this work, we study the effect of TMPyP4 and Methylene Blue on the intrinsic fluorescence of the dimeric G4 DNA structure formed by two d(G3T)4 sequences. We demonstrate that binding of the ligands results in quenching of the intrinsic fluorescence, although the con...
Source: European Biophysics Journal : EBJ - January 13, 2024 Category: Physics Authors: Liana L Tevonyan Artemy D Beniaminov Dmitry N Kaluzhny Source Type: research
Swimming polarity inversion in uncultured magnetotactic cocci
Eur Biophys J. 2024 Feb;53(1-2):69-76. doi: 10.1007/s00249-023-01698-1. Epub 2024 Jan 12.ABSTRACTMagnetotactic bacteria are microorganisms that produce intracellular magnetic nanoparticles organized in chains, conferring a magnetic moment to the bacterial body that allows it to swim following the geomagnetic field lines. Magnetotactic bacteria usually display two swimming polarities in environmental samples: the South-seeking (SS) polarity and the North-seeking (NS) polarity, characterized by the bacteria swimming antiparallel or parallel to the magnetic field lines, respectively. It has been observed that in the presence ...
Source: European Biophysics Journal : EBJ - January 12, 2024 Category: Physics Authors: Giovanny Angiolillo Fernanda Abreu Daniel Acosta-Avalos Source Type: research
Probing the interactions of the HIV-1 matrix protein-derived polybasic region with lipid bilayers: insights from AFM imaging and force spectroscopy
Eur Biophys J. 2024 Feb;53(1-2):57-67. doi: 10.1007/s00249-023-01697-2. Epub 2024 Jan 3.ABSTRACTThe human immunodeficiency virus type 1 (HIV-1) matrix protein contains a highly basic region, MA-HBR, crucial for various stages of viral replication. To elucidate the interactions between the polybasic peptide MA-HBR and lipid bilayers, we employed liquid-based atomic force microscopy (AFM) imaging and force spectroscopy on lipid bilayers of differing compositions. In 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers, AFM imaging revealed the formation of annulus-shaped protrusions upon exposure to the polybasic...
Source: European Biophysics Journal : EBJ - January 3, 2024 Category: Physics Authors: Chinta M Aryal Jianjun Pan Source Type: research
