Mammalian Nudt15 hydrolytic and binding activity on methylated guanosine mononucleotides
Eur Biophys J. 2023 Aug 29. doi: 10.1007/s00249-023-01678-5. Online ahead of print.ABSTRACTThe Nudt15 enzyme of the NUDIX protein family is the subject of extensive study due to its action on thiopurine drugs used in the treatment of cancer and inflammatory diseases. In addition to thiopurines, Nudt15 is enzymatically active in vitro on several nucleotide substrates. It has also been suggested that this enzyme may play a role in 5'RNA turnover by hydrolyzing m7GDP, a product of mRNA decapping. However, no detailed studies on this substrate with Nudt15 are available. Here, we analyzed the enzymatic activity of Nudt15 with m...
Source: European Biophysics Journal : EBJ - August 29, 2023 Category: Physics Authors: Maciej Lukaszewicz Aleksandra Ferenc-Mrozek Julia Kokosza Anna Stefaniuk Janusz Stepinski Elzbieta Bojarska Edward Darzynkiewicz Source Type: research
Mammalian Nudt15 hydrolytic and binding activity on methylated guanosine mononucleotides
Eur Biophys J. 2023 Aug 29. doi: 10.1007/s00249-023-01678-5. Online ahead of print.ABSTRACTThe Nudt15 enzyme of the NUDIX protein family is the subject of extensive study due to its action on thiopurine drugs used in the treatment of cancer and inflammatory diseases. In addition to thiopurines, Nudt15 is enzymatically active in vitro on several nucleotide substrates. It has also been suggested that this enzyme may play a role in 5'RNA turnover by hydrolyzing m7GDP, a product of mRNA decapping. However, no detailed studies on this substrate with Nudt15 are available. Here, we analyzed the enzymatic activity of Nudt15 with m...
Source: European Biophysics Journal : EBJ - August 29, 2023 Category: Physics Authors: Maciej Lukaszewicz Aleksandra Ferenc-Mrozek Julia Kokosza Anna Stefaniuk Janusz Stepinski Elzbieta Bojarska Edward Darzynkiewicz Source Type: research
Mammalian Nudt15 hydrolytic and binding activity on methylated guanosine mononucleotides
Eur Biophys J. 2023 Aug 29. doi: 10.1007/s00249-023-01678-5. Online ahead of print.ABSTRACTThe Nudt15 enzyme of the NUDIX protein family is the subject of extensive study due to its action on thiopurine drugs used in the treatment of cancer and inflammatory diseases. In addition to thiopurines, Nudt15 is enzymatically active in vitro on several nucleotide substrates. It has also been suggested that this enzyme may play a role in 5'RNA turnover by hydrolyzing m7GDP, a product of mRNA decapping. However, no detailed studies on this substrate with Nudt15 are available. Here, we analyzed the enzymatic activity of Nudt15 with m...
Source: European Biophysics Journal : EBJ - August 29, 2023 Category: Physics Authors: Maciej Lukaszewicz Aleksandra Ferenc-Mrozek Julia Kokosza Anna Stefaniuk Janusz Stepinski Elzbieta Bojarska Edward Darzynkiewicz Source Type: research
Structure and dynamics of pteridine reductase 1: the key phenomena relevant to enzyme function and drug design
Eur Biophys J. 2023 Aug 22. doi: 10.1007/s00249-023-01677-6. Online ahead of print.ABSTRACTPteridine reductase 1 (PTR1) is a folate and pterin pathway enzyme unique for pathogenic trypanosomatids. As a validated drug target, PTR1 has been the focus of recent research efforts aimed at finding more effective treatments against human parasitic diseases such as leishmaniasis or sleeping sickness. Previous PTR1-centered structural studies highlighted the enzyme characteristics, such as flexible regions around the active site, highly conserved structural waters, and species-specific differences in pocket properties and dynamics,...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Joanna Panecka-Hofman Ina Poehner Source Type: research
Peptide nucleic acid conjugates and their antimicrobial applications-a mini-review
Eur Biophys J. 2023 Aug 23. doi: 10.1007/s00249-023-01673-w. Online ahead of print.ABSTRACTPeptide nucleic acid (PNA) is a nucleic acid mimic with high specificity and binding affinity to natural DNA or RNA, as well as resistance to enzymatic degradation. PNA sequences can be designed to selectively silence gene expression, which makes PNA a promising tool for antimicrobial applications. However, the poor membrane permeability of PNA remains the main limiting factor for its applications in cells. To overcome this obstacle, PNA conjugates with different molecules have been developed. This mini-review focuses on covalently l...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Uladzislava Tsylents Izabela Siekierska Joanna Trylska Source Type: research
Structure and dynamics of pteridine reductase 1: the key phenomena relevant to enzyme function and drug design
Eur Biophys J. 2023 Aug 22. doi: 10.1007/s00249-023-01677-6. Online ahead of print.ABSTRACTPteridine reductase 1 (PTR1) is a folate and pterin pathway enzyme unique for pathogenic trypanosomatids. As a validated drug target, PTR1 has been the focus of recent research efforts aimed at finding more effective treatments against human parasitic diseases such as leishmaniasis or sleeping sickness. Previous PTR1-centered structural studies highlighted the enzyme characteristics, such as flexible regions around the active site, highly conserved structural waters, and species-specific differences in pocket properties and dynamics,...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Joanna Panecka-Hofman Ina Poehner Source Type: research
Peptide nucleic acid conjugates and their antimicrobial applications-a mini-review
Eur Biophys J. 2023 Aug 23. doi: 10.1007/s00249-023-01673-w. Online ahead of print.ABSTRACTPeptide nucleic acid (PNA) is a nucleic acid mimic with high specificity and binding affinity to natural DNA or RNA, as well as resistance to enzymatic degradation. PNA sequences can be designed to selectively silence gene expression, which makes PNA a promising tool for antimicrobial applications. However, the poor membrane permeability of PNA remains the main limiting factor for its applications in cells. To overcome this obstacle, PNA conjugates with different molecules have been developed. This mini-review focuses on covalently l...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Uladzislava Tsylents Izabela Siekierska Joanna Trylska Source Type: research
Structure and dynamics of pteridine reductase 1: the key phenomena relevant to enzyme function and drug design
Eur Biophys J. 2023 Aug 22. doi: 10.1007/s00249-023-01677-6. Online ahead of print.ABSTRACTPteridine reductase 1 (PTR1) is a folate and pterin pathway enzyme unique for pathogenic trypanosomatids. As a validated drug target, PTR1 has been the focus of recent research efforts aimed at finding more effective treatments against human parasitic diseases such as leishmaniasis or sleeping sickness. Previous PTR1-centered structural studies highlighted the enzyme characteristics, such as flexible regions around the active site, highly conserved structural waters, and species-specific differences in pocket properties and dynamics,...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Joanna Panecka-Hofman Ina Poehner Source Type: research
Peptide nucleic acid conjugates and their antimicrobial applications-a mini-review
Eur Biophys J. 2023 Aug 23. doi: 10.1007/s00249-023-01673-w. Online ahead of print.ABSTRACTPeptide nucleic acid (PNA) is a nucleic acid mimic with high specificity and binding affinity to natural DNA or RNA, as well as resistance to enzymatic degradation. PNA sequences can be designed to selectively silence gene expression, which makes PNA a promising tool for antimicrobial applications. However, the poor membrane permeability of PNA remains the main limiting factor for its applications in cells. To overcome this obstacle, PNA conjugates with different molecules have been developed. This mini-review focuses on covalently l...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Uladzislava Tsylents Izabela Siekierska Joanna Trylska Source Type: research
Structure and dynamics of pteridine reductase 1: the key phenomena relevant to enzyme function and drug design
Eur Biophys J. 2023 Aug 22. doi: 10.1007/s00249-023-01677-6. Online ahead of print.ABSTRACTPteridine reductase 1 (PTR1) is a folate and pterin pathway enzyme unique for pathogenic trypanosomatids. As a validated drug target, PTR1 has been the focus of recent research efforts aimed at finding more effective treatments against human parasitic diseases such as leishmaniasis or sleeping sickness. Previous PTR1-centered structural studies highlighted the enzyme characteristics, such as flexible regions around the active site, highly conserved structural waters, and species-specific differences in pocket properties and dynamics,...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Joanna Panecka-Hofman Ina Poehner Source Type: research
Peptide nucleic acid conjugates and their antimicrobial applications-a mini-review
Eur Biophys J. 2023 Aug 23. doi: 10.1007/s00249-023-01673-w. Online ahead of print.ABSTRACTPeptide nucleic acid (PNA) is a nucleic acid mimic with high specificity and binding affinity to natural DNA or RNA, as well as resistance to enzymatic degradation. PNA sequences can be designed to selectively silence gene expression, which makes PNA a promising tool for antimicrobial applications. However, the poor membrane permeability of PNA remains the main limiting factor for its applications in cells. To overcome this obstacle, PNA conjugates with different molecules have been developed. This mini-review focuses on covalently l...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Uladzislava Tsylents Izabela Siekierska Joanna Trylska Source Type: research
Structure and dynamics of pteridine reductase 1: the key phenomena relevant to enzyme function and drug design
Eur Biophys J. 2023 Aug 22. doi: 10.1007/s00249-023-01677-6. Online ahead of print.ABSTRACTPteridine reductase 1 (PTR1) is a folate and pterin pathway enzyme unique for pathogenic trypanosomatids. As a validated drug target, PTR1 has been the focus of recent research efforts aimed at finding more effective treatments against human parasitic diseases such as leishmaniasis or sleeping sickness. Previous PTR1-centered structural studies highlighted the enzyme characteristics, such as flexible regions around the active site, highly conserved structural waters, and species-specific differences in pocket properties and dynamics,...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Joanna Panecka-Hofman Ina Poehner Source Type: research
Peptide nucleic acid conjugates and their antimicrobial applications-a mini-review
Eur Biophys J. 2023 Aug 23. doi: 10.1007/s00249-023-01673-w. Online ahead of print.ABSTRACTPeptide nucleic acid (PNA) is a nucleic acid mimic with high specificity and binding affinity to natural DNA or RNA, as well as resistance to enzymatic degradation. PNA sequences can be designed to selectively silence gene expression, which makes PNA a promising tool for antimicrobial applications. However, the poor membrane permeability of PNA remains the main limiting factor for its applications in cells. To overcome this obstacle, PNA conjugates with different molecules have been developed. This mini-review focuses on covalently l...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Uladzislava Tsylents Izabela Siekierska Joanna Trylska Source Type: research
Structure and dynamics of pteridine reductase 1: the key phenomena relevant to enzyme function and drug design
Eur Biophys J. 2023 Aug 22. doi: 10.1007/s00249-023-01677-6. Online ahead of print.ABSTRACTPteridine reductase 1 (PTR1) is a folate and pterin pathway enzyme unique for pathogenic trypanosomatids. As a validated drug target, PTR1 has been the focus of recent research efforts aimed at finding more effective treatments against human parasitic diseases such as leishmaniasis or sleeping sickness. Previous PTR1-centered structural studies highlighted the enzyme characteristics, such as flexible regions around the active site, highly conserved structural waters, and species-specific differences in pocket properties and dynamics,...
Source: European Biophysics Journal : EBJ - August 23, 2023 Category: Physics Authors: Joanna Panecka-Hofman Ina Poehner Source Type: research
Long-term memory in Staphylococcus aureus α-hemolysin ion channel kinetics
Eur Biophys J. 2023 Aug 5. doi: 10.1007/s00249-023-01675-8. Online ahead of print.ABSTRACTThe kinetics of an ion channel are classically understood as a random process. However, studies have shown that in complex ion channels, formed by multiple subunits, this process can be deterministic, presenting long-term memory. Staphylococcus aureus α-hemolysin (α-HL) is a toxin that acts as the major factor in Staphylococcus aureus virulence. α-HL is a water-soluble protein capable of forming ion channels into lipid bilayers, by insertion of an amphipathic β-barrel. Here, the α-HL was used as an experimental model to study mem...
Source: European Biophysics Journal : EBJ - August 5, 2023 Category: Physics Authors: M P Silva C G Rodrigues D C Machado R A Nogueira Source Type: research
