Proceedings of the 25th Analytical Ultracentrifugation Workshops and Symposium
Eur Biophys J. 2023 Aug 1. doi: 10.1007/s00249-023-01674-9. Online ahead of print.ABSTRACTThe 25th International Analytical Ultracentrifugation (AUC) Workshops and Symposium (AUC2022) took place at the University of Lethbridge in Lethbridge, Canada, in July 2022. In total, 104 attendees (Attendance Profile: 104 attendees, 69 in-person, 35 remote. Brazil 1, Canada 24, China 1, Czech Republic 2, Finland 1, France 3, Germany 22, India 3, Italy 1, Japan 4, Spain 1, Switzerland 3, Taiwan 1, United Kingdom 5, United States 32) participated in the event and presented the latest advances in the field. While the primary focus of th...
Source: European Biophysics Journal : EBJ - August 1, 2023 Category: Physics Authors: Borries Demeler Robert Gilbert Trushar R Patel Source Type: research
Tautomeric equilibrium and spectroscopic properties of 8-azaguanine revealed by quantum chemistry methods
Eur Biophys J. 2023 Jul 28. doi: 10.1007/s00249-023-01672-x. Online ahead of print.ABSTRACT8-azaguanine is a triazolopyrimidine nucleobase analog possessing potent antibacterial and antitumor activities, and it has been implicated as a lead molecule in cancer and malaria therapy. Its intrinsic fluorescence properties can be utilized for monitoring its interactions with biological polymers like proteins or nucleic acids. In order to better understand these interactions, it is important to know the tautomeric equilibrium of this compound. In this work, the tautomeric equilibrium of all natural neutral and anionic compound fo...
Source: European Biophysics Journal : EBJ - July 28, 2023 Category: Physics Authors: Maciej Maciejczyk Maciej Pyrka Source Type: research
Tautomeric equilibrium and spectroscopic properties of 8-azaguanine revealed by quantum chemistry methods
Eur Biophys J. 2023 Jul 28. doi: 10.1007/s00249-023-01672-x. Online ahead of print.ABSTRACT8-azaguanine is a triazolopyrimidine nucleobase analog possessing potent antibacterial and antitumor activities, and it has been implicated as a lead molecule in cancer and malaria therapy. Its intrinsic fluorescence properties can be utilized for monitoring its interactions with biological polymers like proteins or nucleic acids. In order to better understand these interactions, it is important to know the tautomeric equilibrium of this compound. In this work, the tautomeric equilibrium of all natural neutral and anionic compound fo...
Source: European Biophysics Journal : EBJ - July 28, 2023 Category: Physics Authors: Maciej Maciejczyk Maciej Pyrka Source Type: research
Tautomeric equilibrium and spectroscopic properties of 8-azaguanine revealed by quantum chemistry methods
Eur Biophys J. 2023 Jul 28. doi: 10.1007/s00249-023-01672-x. Online ahead of print.ABSTRACT8-azaguanine is a triazolopyrimidine nucleobase analog possessing potent antibacterial and antitumor activities, and it has been implicated as a lead molecule in cancer and malaria therapy. Its intrinsic fluorescence properties can be utilized for monitoring its interactions with biological polymers like proteins or nucleic acids. In order to better understand these interactions, it is important to know the tautomeric equilibrium of this compound. In this work, the tautomeric equilibrium of all natural neutral and anionic compound fo...
Source: European Biophysics Journal : EBJ - July 28, 2023 Category: Physics Authors: Maciej Maciejczyk Maciej Pyrka Source Type: research
Suitability of double-stranded DNA as a molecular standard for the validation of analytical ultracentrifugation instruments
Eur Biophys J. 2023 Jul 27. doi: 10.1007/s00249-023-01671-y. Online ahead of print.ABSTRACTTo address the current lack of validated molecular standards for analytical ultracentrifugation (AUC), we investigated the suitability of double-stranded DNA molecules. We compared the hydrodynamic properties of linear and circular DNA as a function of temperature. Negatively supercoiled, nicked, and linearized 333 and 339 bp minicircles were studied. We quantified the hydrodynamic properties of these DNAs at five different temperatures, ranging from 4 to 37 °C. To enhance the precision of our measurements, each sample was globally ...
Source: European Biophysics Journal : EBJ - July 27, 2023 Category: Physics Authors: Maduni Ranasinghe Jonathan M Fogg Daniel J Catanese Lynn Zechiedrich Borries Demeler Source Type: research
Suitability of double-stranded DNA as a molecular standard for the validation of analytical ultracentrifugation instruments
Eur Biophys J. 2023 Jul 27. doi: 10.1007/s00249-023-01671-y. Online ahead of print.ABSTRACTTo address the current lack of validated molecular standards for analytical ultracentrifugation (AUC), we investigated the suitability of double-stranded DNA molecules. We compared the hydrodynamic properties of linear and circular DNA as a function of temperature. Negatively supercoiled, nicked, and linearized 333 and 339 bp minicircles were studied. We quantified the hydrodynamic properties of these DNAs at five different temperatures, ranging from 4 to 37 °C. To enhance the precision of our measurements, each sample was globally ...
Source: European Biophysics Journal : EBJ - July 27, 2023 Category: Physics Authors: Maduni Ranasinghe Jonathan M Fogg Daniel J Catanese Lynn Zechiedrich Borries Demeler Source Type: research
Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter
Eur Biophys J. 2023 Jul 18. doi: 10.1007/s00249-023-01665-w. Online ahead of print.ABSTRACTThis investigation examines the source of the disparity between experimental values of the light scattering second virial coefficient [Formula: see text] (mL.mol/g2) for proteins and those predicted on the statistical mechanical basis of excluded volume. A much better theoretical description of published results for lysozyme is obtained by considering the experimental parameters to monitor the difference between the thermodynamic excluded volume term and its hydrodynamic counterpart. This involves a combination of parameters quantify...
Source: European Biophysics Journal : EBJ - July 17, 2023 Category: Physics Authors: Donald J Winzor Vlad Dinu David J Scott Stephen E Harding Source Type: research
Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter
Eur Biophys J. 2023 Jul 18. doi: 10.1007/s00249-023-01665-w. Online ahead of print.ABSTRACTThis investigation examines the source of the disparity between experimental values of the light scattering second virial coefficient [Formula: see text] (mL.mol/g2) for proteins and those predicted on the statistical mechanical basis of excluded volume. A much better theoretical description of published results for lysozyme is obtained by considering the experimental parameters to monitor the difference between the thermodynamic excluded volume term and its hydrodynamic counterpart. This involves a combination of parameters quantify...
Source: European Biophysics Journal : EBJ - July 17, 2023 Category: Physics Authors: Donald J Winzor Vlad Dinu David J Scott Stephen E Harding Source Type: research
Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter
Eur Biophys J. 2023 Jul 18. doi: 10.1007/s00249-023-01665-w. Online ahead of print.ABSTRACTThis investigation examines the source of the disparity between experimental values of the light scattering second virial coefficient [Formula: see text] (mL.mol/g2) for proteins and those predicted on the statistical mechanical basis of excluded volume. A much better theoretical description of published results for lysozyme is obtained by considering the experimental parameters to monitor the difference between the thermodynamic excluded volume term and its hydrodynamic counterpart. This involves a combination of parameters quantify...
Source: European Biophysics Journal : EBJ - July 17, 2023 Category: Physics Authors: Donald J Winzor Vlad Dinu David J Scott Stephen E Harding Source Type: research
Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter
Eur Biophys J. 2023 Jul 18. doi: 10.1007/s00249-023-01665-w. Online ahead of print.ABSTRACTThis investigation examines the source of the disparity between experimental values of the light scattering second virial coefficient [Formula: see text] (mL.mol/g2) for proteins and those predicted on the statistical mechanical basis of excluded volume. A much better theoretical description of published results for lysozyme is obtained by considering the experimental parameters to monitor the difference between the thermodynamic excluded volume term and its hydrodynamic counterpart. This involves a combination of parameters quantify...
Source: European Biophysics Journal : EBJ - July 17, 2023 Category: Physics Authors: Donald J Winzor Vlad Dinu David J Scott Stephen E Harding Source Type: research
Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter
Eur Biophys J. 2023 Jul 18. doi: 10.1007/s00249-023-01665-w. Online ahead of print.ABSTRACTThis investigation examines the source of the disparity between experimental values of the light scattering second virial coefficient [Formula: see text] (mL.mol/g2) for proteins and those predicted on the statistical mechanical basis of excluded volume. A much better theoretical description of published results for lysozyme is obtained by considering the experimental parameters to monitor the difference between the thermodynamic excluded volume term and its hydrodynamic counterpart. This involves a combination of parameters quantify...
Source: European Biophysics Journal : EBJ - July 17, 2023 Category: Physics Authors: Donald J Winzor Vlad Dinu David J Scott Stephen E Harding Source Type: research
Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter
Eur Biophys J. 2023 Jul 18. doi: 10.1007/s00249-023-01665-w. Online ahead of print.ABSTRACTThis investigation examines the source of the disparity between experimental values of the light scattering second virial coefficient [Formula: see text] (mL.mol/g2) for proteins and those predicted on the statistical mechanical basis of excluded volume. A much better theoretical description of published results for lysozyme is obtained by considering the experimental parameters to monitor the difference between the thermodynamic excluded volume term and its hydrodynamic counterpart. This involves a combination of parameters quantify...
Source: European Biophysics Journal : EBJ - July 17, 2023 Category: Physics Authors: Donald J Winzor Vlad Dinu David J Scott Stephen E Harding Source Type: research
Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter
Eur Biophys J. 2023 Jul 18. doi: 10.1007/s00249-023-01665-w. Online ahead of print.ABSTRACTThis investigation examines the source of the disparity between experimental values of the light scattering second virial coefficient [Formula: see text] (mL.mol/g2) for proteins and those predicted on the statistical mechanical basis of excluded volume. A much better theoretical description of published results for lysozyme is obtained by considering the experimental parameters to monitor the difference between the thermodynamic excluded volume term and its hydrodynamic counterpart. This involves a combination of parameters quantify...
Source: European Biophysics Journal : EBJ - July 17, 2023 Category: Physics Authors: Donald J Winzor Vlad Dinu David J Scott Stephen E Harding Source Type: research
Experimental support for reclassification of the light scattering second virial coefficient from macromolecular solutions as a hydrodynamic parameter
Eur Biophys J. 2023 Jul 18. doi: 10.1007/s00249-023-01665-w. Online ahead of print.ABSTRACTThis investigation examines the source of the disparity between experimental values of the light scattering second virial coefficient [Formula: see text] (mL.mol/g2) for proteins and those predicted on the statistical mechanical basis of excluded volume. A much better theoretical description of published results for lysozyme is obtained by considering the experimental parameters to monitor the difference between the thermodynamic excluded volume term and its hydrodynamic counterpart. This involves a combination of parameters quantify...
Source: European Biophysics Journal : EBJ - July 17, 2023 Category: Physics Authors: Donald J Winzor Vlad Dinu David J Scott Stephen E Harding Source Type: research
14th EBSA congress, July 31 - August 4, 2023, Stockholm, Sweden - Abstracts
Eur Biophys J. 2023 Jul;52(Suppl 1):1-220. doi: 10.1007/s00249-023-01668-7.NO ABSTRACTPMID:37436453 | DOI:10.1007/s00249-023-01668-7 (Source: European Biophysics Journal : EBJ)
Source: European Biophysics Journal : EBJ - July 12, 2023 Category: Physics Source Type: research
