Structure of a novel form of phosphopantetheine adenylyltransferase from Klebsiella pneumoniae at 2.59 Å resolution
Eur Biophys J. 2024 Mar 8. doi: 10.1007/s00249-024-01703-1. Online ahead of print.ABSTRACTPhosphopantetheine adenylyltransferase (EC. 2.7.7.3, PPAT) catalyzes the penultimate step of the multistep reaction in the coenzyme A (CoA) biosynthesis pathway. In this step, an adenylyl group from adenosine triphosphate (ATP) is transferred to 4'-phosphopantetheine (PNS) yielding 3'-dephospho-coenzyme A (dpCoA) and pyrophosphate (PPi). PPAT from strain C3 of Klebsiella pneumoniae (KpPPAT) was cloned, expressed and purified. It was crystallized using 0.1 M HEPES buffer and PEG10000 at pH 7.5. The crystals belonged to tetragonal space...
Source: European Biophysics Journal : EBJ - March 8, 2024 Category: Physics Authors: Nabeel Ahmad Pradeep Sharma Sujata Sharma Tej P Singh Source Type: research
Structure of a novel form of phosphopantetheine adenylyltransferase from Klebsiella pneumoniae at 2.59 Å resolution
Eur Biophys J. 2024 Mar 8. doi: 10.1007/s00249-024-01703-1. Online ahead of print.ABSTRACTPhosphopantetheine adenylyltransferase (EC. 2.7.7.3, PPAT) catalyzes the penultimate step of the multistep reaction in the coenzyme A (CoA) biosynthesis pathway. In this step, an adenylyl group from adenosine triphosphate (ATP) is transferred to 4'-phosphopantetheine (PNS) yielding 3'-dephospho-coenzyme A (dpCoA) and pyrophosphate (PPi). PPAT from strain C3 of Klebsiella pneumoniae (KpPPAT) was cloned, expressed and purified. It was crystallized using 0.1 M HEPES buffer and PEG10000 at pH 7.5. The crystals belonged to tetragonal space...
Source: European Biophysics Journal : EBJ - March 8, 2024 Category: Physics Authors: Nabeel Ahmad Pradeep Sharma Sujata Sharma Tej P Singh Source Type: research
Structure of a novel form of phosphopantetheine adenylyltransferase from Klebsiella pneumoniae at 2.59 Å resolution
Eur Biophys J. 2024 Mar 8. doi: 10.1007/s00249-024-01703-1. Online ahead of print.ABSTRACTPhosphopantetheine adenylyltransferase (EC. 2.7.7.3, PPAT) catalyzes the penultimate step of the multistep reaction in the coenzyme A (CoA) biosynthesis pathway. In this step, an adenylyl group from adenosine triphosphate (ATP) is transferred to 4'-phosphopantetheine (PNS) yielding 3'-dephospho-coenzyme A (dpCoA) and pyrophosphate (PPi). PPAT from strain C3 of Klebsiella pneumoniae (KpPPAT) was cloned, expressed and purified. It was crystallized using 0.1 M HEPES buffer and PEG10000 at pH 7.5. The crystals belonged to tetragonal space...
Source: European Biophysics Journal : EBJ - March 8, 2024 Category: Physics Authors: Nabeel Ahmad Pradeep Sharma Sujata Sharma Tej P Singh Source Type: research
Structure of a novel form of phosphopantetheine adenylyltransferase from Klebsiella pneumoniae at 2.59 Å resolution
Eur Biophys J. 2024 Mar 8. doi: 10.1007/s00249-024-01703-1. Online ahead of print.ABSTRACTPhosphopantetheine adenylyltransferase (EC. 2.7.7.3, PPAT) catalyzes the penultimate step of the multistep reaction in the coenzyme A (CoA) biosynthesis pathway. In this step, an adenylyl group from adenosine triphosphate (ATP) is transferred to 4'-phosphopantetheine (PNS) yielding 3'-dephospho-coenzyme A (dpCoA) and pyrophosphate (PPi). PPAT from strain C3 of Klebsiella pneumoniae (KpPPAT) was cloned, expressed and purified. It was crystallized using 0.1 M HEPES buffer and PEG10000 at pH 7.5. The crystals belonged to tetragonal space...
Source: European Biophysics Journal : EBJ - March 8, 2024 Category: Physics Authors: Nabeel Ahmad Pradeep Sharma Sujata Sharma Tej P Singh Source Type: research
Structure of a novel form of phosphopantetheine adenylyltransferase from Klebsiella pneumoniae at 2.59 Å resolution
Eur Biophys J. 2024 Mar 8. doi: 10.1007/s00249-024-01703-1. Online ahead of print.ABSTRACTPhosphopantetheine adenylyltransferase (EC. 2.7.7.3, PPAT) catalyzes the penultimate step of the multistep reaction in the coenzyme A (CoA) biosynthesis pathway. In this step, an adenylyl group from adenosine triphosphate (ATP) is transferred to 4'-phosphopantetheine (PNS) yielding 3'-dephospho-coenzyme A (dpCoA) and pyrophosphate (PPi). PPAT from strain C3 of Klebsiella pneumoniae (KpPPAT) was cloned, expressed and purified. It was crystallized using 0.1 M HEPES buffer and PEG10000 at pH 7.5. The crystals belonged to tetragonal space...
Source: European Biophysics Journal : EBJ - March 8, 2024 Category: Physics Authors: Nabeel Ahmad Pradeep Sharma Sujata Sharma Tej P Singh Source Type: research
Structure of a novel form of phosphopantetheine adenylyltransferase from Klebsiella pneumoniae at 2.59 Å resolution
Eur Biophys J. 2024 Mar 8. doi: 10.1007/s00249-024-01703-1. Online ahead of print.ABSTRACTPhosphopantetheine adenylyltransferase (EC. 2.7.7.3, PPAT) catalyzes the penultimate step of the multistep reaction in the coenzyme A (CoA) biosynthesis pathway. In this step, an adenylyl group from adenosine triphosphate (ATP) is transferred to 4'-phosphopantetheine (PNS) yielding 3'-dephospho-coenzyme A (dpCoA) and pyrophosphate (PPi). PPAT from strain C3 of Klebsiella pneumoniae (KpPPAT) was cloned, expressed and purified. It was crystallized using 0.1 M HEPES buffer and PEG10000 at pH 7.5. The crystals belonged to tetragonal space...
Source: European Biophysics Journal : EBJ - March 8, 2024 Category: Physics Authors: Nabeel Ahmad Pradeep Sharma Sujata Sharma Tej P Singh Source Type: research
Observation of magnet-induced star-like radiation of a plasma created from cancer cells in a laser trap
We present a new phenomenon resulting from the interaction of magnetic beads with cancer cells in a laser trap formed on a slide containing a depression 16.5 mm in diameter and 0.78 mm of maximum depth. This phenomenon includes the apparent formation and expansion of a dark bubble that attracts and incinerates surrounding matter when it explodes, which leads to a plasma emitting intense radiation that has the appearance of a star on a microscopic scale. We have observed the star-like phenomenon for more than 4 years, and the intensity depends on the laser's power. Measuring the laser power of the dark bubble shows the entr...
Source: European Biophysics Journal : EBJ - March 7, 2024 Category: Physics Authors: D Erenso L Tran I Abualrob M Bushra J Hengstenberg E Muhammed I Endale N Endale E Endale S Mayhut N Torres P Sheffield C Vazquez H Crogman C Nichols T Dang E E Hach Source Type: research
Ultraviolet C intensity dependence of decontamination efficiency for pathogens as function of repacked metamaterials with screw channels
Eur Biophys J. 2024 Feb 28. doi: 10.1007/s00249-024-01702-2. Online ahead of print.ABSTRACTA new method for repackaging optical metamaterials formed from quartz spheres (fibers) of various diameters is proposed for ultraviolet C disinfection of infected liquids by pathogens (viruses and bacteria). The main idea of the new equipment is connected with the rotation of a contaminated fluid by screw channels within a metamaterial matrix prepared from UVC fibers/spherical optics, to improve the decontamination efficiency. In demonstration of the viability of this approach, dynamic and static inactivation of Baker's yeast via Ult...
Source: European Biophysics Journal : EBJ - February 28, 2024 Category: Physics Authors: Ion Munteanu Elena Starodub Sergiu Bazgan Marina Turcan Tatiana Paslari Diana Podoleanu Nicolae A Enaki Source Type: research
Ultraviolet C intensity dependence of decontamination efficiency for pathogens as function of repacked metamaterials with screw channels
Eur Biophys J. 2024 Feb 28. doi: 10.1007/s00249-024-01702-2. Online ahead of print.ABSTRACTA new method for repackaging optical metamaterials formed from quartz spheres (fibers) of various diameters is proposed for ultraviolet C disinfection of infected liquids by pathogens (viruses and bacteria). The main idea of the new equipment is connected with the rotation of a contaminated fluid by screw channels within a metamaterial matrix prepared from UVC fibers/spherical optics, to improve the decontamination efficiency. In demonstration of the viability of this approach, dynamic and static inactivation of Baker's yeast via Ult...
Source: European Biophysics Journal : EBJ - February 28, 2024 Category: Physics Authors: Ion Munteanu Elena Starodub Sergiu Bazgan Marina Turcan Tatiana Paslari Diana Podoleanu Nicolae A Enaki Source Type: research
Ultraviolet C intensity dependence of decontamination efficiency for pathogens as function of repacked metamaterials with screw channels
Eur Biophys J. 2024 Feb 28. doi: 10.1007/s00249-024-01702-2. Online ahead of print.ABSTRACTA new method for repackaging optical metamaterials formed from quartz spheres (fibers) of various diameters is proposed for ultraviolet C disinfection of infected liquids by pathogens (viruses and bacteria). The main idea of the new equipment is connected with the rotation of a contaminated fluid by screw channels within a metamaterial matrix prepared from UVC fibers/spherical optics, to improve the decontamination efficiency. In demonstration of the viability of this approach, dynamic and static inactivation of Baker's yeast via Ult...
Source: European Biophysics Journal : EBJ - February 28, 2024 Category: Physics Authors: Ion Munteanu Elena Starodub Sergiu Bazgan Marina Turcan Tatiana Paslari Diana Podoleanu Nicolae A Enaki Source Type: research
Ultraviolet C intensity dependence of decontamination efficiency for pathogens as function of repacked metamaterials with screw channels
Eur Biophys J. 2024 Feb 28. doi: 10.1007/s00249-024-01702-2. Online ahead of print.ABSTRACTA new method for repackaging optical metamaterials formed from quartz spheres (fibers) of various diameters is proposed for ultraviolet C disinfection of infected liquids by pathogens (viruses and bacteria). The main idea of the new equipment is connected with the rotation of a contaminated fluid by screw channels within a metamaterial matrix prepared from UVC fibers/spherical optics, to improve the decontamination efficiency. In demonstration of the viability of this approach, dynamic and static inactivation of Baker's yeast via Ult...
Source: European Biophysics Journal : EBJ - February 28, 2024 Category: Physics Authors: Ion Munteanu Elena Starodub Sergiu Bazgan Marina Turcan Tatiana Paslari Diana Podoleanu Nicolae A Enaki Source Type: research
Ultraviolet C intensity dependence of decontamination efficiency for pathogens as function of repacked metamaterials with screw channels
Eur Biophys J. 2024 Feb 28. doi: 10.1007/s00249-024-01702-2. Online ahead of print.ABSTRACTA new method for repackaging optical metamaterials formed from quartz spheres (fibers) of various diameters is proposed for ultraviolet C disinfection of infected liquids by pathogens (viruses and bacteria). The main idea of the new equipment is connected with the rotation of a contaminated fluid by screw channels within a metamaterial matrix prepared from UVC fibers/spherical optics, to improve the decontamination efficiency. In demonstration of the viability of this approach, dynamic and static inactivation of Baker's yeast via Ult...
Source: European Biophysics Journal : EBJ - February 28, 2024 Category: Physics Authors: Ion Munteanu Elena Starodub Sergiu Bazgan Marina Turcan Tatiana Paslari Diana Podoleanu Nicolae A Enaki Source Type: research
Ultraviolet C intensity dependence of decontamination efficiency for pathogens as function of repacked metamaterials with screw channels
Eur Biophys J. 2024 Feb 28. doi: 10.1007/s00249-024-01702-2. Online ahead of print.ABSTRACTA new method for repackaging optical metamaterials formed from quartz spheres (fibers) of various diameters is proposed for ultraviolet C disinfection of infected liquids by pathogens (viruses and bacteria). The main idea of the new equipment is connected with the rotation of a contaminated fluid by screw channels within a metamaterial matrix prepared from UVC fibers/spherical optics, to improve the decontamination efficiency. In demonstration of the viability of this approach, dynamic and static inactivation of Baker's yeast via Ult...
Source: European Biophysics Journal : EBJ - February 28, 2024 Category: Physics Authors: Ion Munteanu Elena Starodub Sergiu Bazgan Marina Turcan Tatiana Paslari Diana Podoleanu Nicolae A Enaki Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
BASIS: BioAnalysis SEDFIT integrated software for cGMP analysis of SV-AUC data
Eur Biophys J. 2024 Feb 8. doi: 10.1007/s00249-024-01700-4. Online ahead of print.ABSTRACTSedimentation velocity analytical ultracentrifugation (SV-AUC) has long been an important method for characterization of antibody therapeutics. Recently, SV-AUC has experienced a wave of new interest and usage from the gene and cell therapy industry, where SV-AUC has proven itself to be the "gold standard" analytical approach for determining capsid loading ratios for adeno-associated virus (AAV) and other viral vectors. While other more common approaches have existed in the realm of cGMP-compliant techniques for years, SV-AUC has long...
Source: European Biophysics Journal : EBJ - February 8, 2024 Category: Physics Authors: Alexander E Yarawsky Erik S Gough Valeria Zai-Rose Natalya I Figueroa Hazel M Cunningham John W Burgner Michael T DeLion Lake N Paul Source Type: research
