A model for ribosome translocation based on the alternated displacement of its subunits
Eur Biophys J. 2023 Apr;52(3):175-187. doi: 10.1007/s00249-023-01662-z. Epub 2023 Jun 9.ABSTRACTA meaningful dilemma in ribosome translocation arising from experimental facts is that, although the ribosome-mRNA interaction force always has a significant magnitude, the ribosome still moves to the next codon on the mRNA. How does the ribosome move to the next codon in the sequence while holding the mRNA tightly? The hypothesis proposed here is that ribosome subunits alternate the grip of the ribosome on the mRNA, freeing the other subunit of such interaction for a while, thus allowing its motion to the following codon. Based...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Jos é S González-García Source Type: research
Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
Eur Biophys J. 2023 Jun 8:1-8. doi: 10.1007/s00249-023-01663-y. Online ahead of print.ABSTRACTLipid nanoparticles as delivery system for mRNA have recently attracted attention to a broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to deliver a variety of nucleic acids renders them an interesting and complementary alternative to gene therapy vectors like AAVs. An important quality attribute of LNPs is the copy number of the encapsulated cargo molecule. This work describes how density and molecular weight distributions obtained by density contrast sedimentation velocity can be used to calcul...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Alexander Bepperling Gesa Richter Source Type: research
A model for ribosome translocation based on the alternated displacement of its subunits
Eur Biophys J. 2023 Apr;52(3):175-187. doi: 10.1007/s00249-023-01662-z. Epub 2023 Jun 9.ABSTRACTA meaningful dilemma in ribosome translocation arising from experimental facts is that, although the ribosome-mRNA interaction force always has a significant magnitude, the ribosome still moves to the next codon on the mRNA. How does the ribosome move to the next codon in the sequence while holding the mRNA tightly? The hypothesis proposed here is that ribosome subunits alternate the grip of the ribosome on the mRNA, freeing the other subunit of such interaction for a while, thus allowing its motion to the following codon. Based...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Jos é S González-García Source Type: research
Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
Eur Biophys J. 2023 Jun 8:1-8. doi: 10.1007/s00249-023-01663-y. Online ahead of print.ABSTRACTLipid nanoparticles as delivery system for mRNA have recently attracted attention to a broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to deliver a variety of nucleic acids renders them an interesting and complementary alternative to gene therapy vectors like AAVs. An important quality attribute of LNPs is the copy number of the encapsulated cargo molecule. This work describes how density and molecular weight distributions obtained by density contrast sedimentation velocity can be used to calcul...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Alexander Bepperling Gesa Richter Source Type: research
A model for ribosome translocation based on the alternated displacement of its subunits
Eur Biophys J. 2023 Apr;52(3):175-187. doi: 10.1007/s00249-023-01662-z. Epub 2023 Jun 9.ABSTRACTA meaningful dilemma in ribosome translocation arising from experimental facts is that, although the ribosome-mRNA interaction force always has a significant magnitude, the ribosome still moves to the next codon on the mRNA. How does the ribosome move to the next codon in the sequence while holding the mRNA tightly? The hypothesis proposed here is that ribosome subunits alternate the grip of the ribosome on the mRNA, freeing the other subunit of such interaction for a while, thus allowing its motion to the following codon. Based...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Jos é S González-García Source Type: research
Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
Eur Biophys J. 2023 Jun 8:1-8. doi: 10.1007/s00249-023-01663-y. Online ahead of print.ABSTRACTLipid nanoparticles as delivery system for mRNA have recently attracted attention to a broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to deliver a variety of nucleic acids renders them an interesting and complementary alternative to gene therapy vectors like AAVs. An important quality attribute of LNPs is the copy number of the encapsulated cargo molecule. This work describes how density and molecular weight distributions obtained by density contrast sedimentation velocity can be used to calcul...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Alexander Bepperling Gesa Richter Source Type: research
A model for ribosome translocation based on the alternated displacement of its subunits
Eur Biophys J. 2023 Jun 9. doi: 10.1007/s00249-023-01662-z. Online ahead of print.ABSTRACTA meaningful dilemma in ribosome translocation arising from experimental facts is that, although the ribosome-mRNA interaction force always has a significant magnitude, the ribosome still moves to the next codon on the mRNA. How does the ribosome move to the next codon in the sequence while holding the mRNA tightly? The hypothesis proposed here is that ribosome subunits alternate the grip of the ribosome on the mRNA, freeing the other subunit of such interaction for a while, thus allowing its motion to the following codon. Based on th...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Jos é S González-García Source Type: research
Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
Eur Biophys J. 2023 Jun 8:1-8. doi: 10.1007/s00249-023-01663-y. Online ahead of print.ABSTRACTLipid nanoparticles as delivery system for mRNA have recently attracted attention to a broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to deliver a variety of nucleic acids renders them an interesting and complementary alternative to gene therapy vectors like AAVs. An important quality attribute of LNPs is the copy number of the encapsulated cargo molecule. This work describes how density and molecular weight distributions obtained by density contrast sedimentation velocity can be used to calcul...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Alexander Bepperling Gesa Richter Source Type: research
A model for ribosome translocation based on the alternated displacement of its subunits
Eur Biophys J. 2023 Jun 9. doi: 10.1007/s00249-023-01662-z. Online ahead of print.ABSTRACTA meaningful dilemma in ribosome translocation arising from experimental facts is that, although the ribosome-mRNA interaction force always has a significant magnitude, the ribosome still moves to the next codon on the mRNA. How does the ribosome move to the next codon in the sequence while holding the mRNA tightly? The hypothesis proposed here is that ribosome subunits alternate the grip of the ribosome on the mRNA, freeing the other subunit of such interaction for a while, thus allowing its motion to the following codon. Based on th...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Jos é S González-García Source Type: research
Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
Eur Biophys J. 2023 Jun 8. doi: 10.1007/s00249-023-01663-y. Online ahead of print.ABSTRACTLipid nanoparticles as delivery system for mRNA have recently attracted attention to a broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to deliver a variety of nucleic acids renders them an interesting and complementary alternative to gene therapy vectors like AAVs. An important quality attribute of LNPs is the copy number of the encapsulated cargo molecule. This work describes how density and molecular weight distributions obtained by density contrast sedimentation velocity can be used to calculate ...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Alexander Bepperling Gesa Richter Source Type: research
A model for ribosome translocation based on the alternated displacement of its subunits
Eur Biophys J. 2023 Jun 9. doi: 10.1007/s00249-023-01662-z. Online ahead of print.ABSTRACTA meaningful dilemma in ribosome translocation arising from experimental facts is that, although the ribosome-mRNA interaction force always has a significant magnitude, the ribosome still moves to the next codon on the mRNA. How does the ribosome move to the next codon in the sequence while holding the mRNA tightly? The hypothesis proposed here is that ribosome subunits alternate the grip of the ribosome on the mRNA, freeing the other subunit of such interaction for a while, thus allowing its motion to the following codon. Based on th...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Jos é S González-García Source Type: research
Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
Eur Biophys J. 2023 Jun 8. doi: 10.1007/s00249-023-01663-y. Online ahead of print.ABSTRACTLipid nanoparticles as delivery system for mRNA have recently attracted attention to a broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to deliver a variety of nucleic acids renders them an interesting and complementary alternative to gene therapy vectors like AAVs. An important quality attribute of LNPs is the copy number of the encapsulated cargo molecule. This work describes how density and molecular weight distributions obtained by density contrast sedimentation velocity can be used to calculate ...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Alexander Bepperling Gesa Richter Source Type: research
A model for ribosome translocation based on the alternated displacement of its subunits
Eur Biophys J. 2023 Jun 9. doi: 10.1007/s00249-023-01662-z. Online ahead of print.ABSTRACTA meaningful dilemma in ribosome translocation arising from experimental facts is that, although the ribosome-mRNA interaction force always has a significant magnitude, the ribosome still moves to the next codon on the mRNA. How does the ribosome move to the next codon in the sequence while holding the mRNA tightly? The hypothesis proposed here is that ribosome subunits alternate the grip of the ribosome on the mRNA, freeing the other subunit of such interaction for a while, thus allowing its motion to the following codon. Based on th...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Jos é S González-García Source Type: research
Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
Eur Biophys J. 2023 Jun 8. doi: 10.1007/s00249-023-01663-y. Online ahead of print.ABSTRACTLipid nanoparticles as delivery system for mRNA have recently attracted attention to a broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to deliver a variety of nucleic acids renders them an interesting and complementary alternative to gene therapy vectors like AAVs. An important quality attribute of LNPs is the copy number of the encapsulated cargo molecule. This work describes how density and molecular weight distributions obtained by density contrast sedimentation velocity can be used to calculate ...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Alexander Bepperling Gesa Richter Source Type: research
A model for ribosome translocation based on the alternated displacement of its subunits
Eur Biophys J. 2023 Jun 9. doi: 10.1007/s00249-023-01662-z. Online ahead of print.ABSTRACTA meaningful dilemma in ribosome translocation arising from experimental facts is that, although the ribosome-mRNA interaction force always has a significant magnitude, the ribosome still moves to the next codon on the mRNA. How does the ribosome move to the next codon in the sequence while holding the mRNA tightly? The hypothesis proposed here is that ribosome subunits alternate the grip of the ribosome on the mRNA, freeing the other subunit of such interaction for a while, thus allowing its motion to the following codon. Based on th...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Jos é S González-García Source Type: research
