SDS-induced hexameric oligomerization of myotoxin-II from Bothrops asper assessed by sedimentation velocity and nuclear magnetic resonance
We report the solution behavior, oligomerization state, and structural details of myotoxin-II purified from the venom of Bothrops asper in the presence and absence of sodium dodecyl sulfate (SDS) and multiple lipids, as examined by analytical ultracentrifugation and nuclear magnetic resonance. Molecular functional and structural details of the myotoxic mechanism of group II Lys-49 phospholipase A2 homologues have been only partially elucidated so far, and conflicting observations have been reported in the literature regarding the monomeric vs. oligomeric state of these toxins in solution. We observed the formation of a sta...
Source: European Biophysics Journal : EBJ - May 20, 2023 Category: Physics Authors: Amy Henrickson Tony Montina Paul Hazendonk Bruno Lomonte Ana Gisele C Neves-Ferreira Borries Demeler Source Type: research
SDS-induced hexameric oligomerization of myotoxin-II from Bothrops asper assessed by sedimentation velocity and nuclear magnetic resonance
We report the solution behavior, oligomerization state, and structural details of myotoxin-II purified from the venom of Bothrops asper in the presence and absence of sodium dodecyl sulfate (SDS) and multiple lipids, as examined by analytical ultracentrifugation and nuclear magnetic resonance. Molecular functional and structural details of the myotoxic mechanism of group II Lys-49 phospholipase A2 homologues have been only partially elucidated so far, and conflicting observations have been reported in the literature regarding the monomeric vs. oligomeric state of these toxins in solution. We observed the formation of a sta...
Source: European Biophysics Journal : EBJ - May 20, 2023 Category: Physics Authors: Amy Henrickson Tony Montina Paul Hazendonk Bruno Lomonte Ana Gisele C Neves-Ferreira Borries Demeler Source Type: research
Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid
Eur Biophys J. 2023 May 17. doi: 10.1007/s00249-023-01656-x. Online ahead of print.ABSTRACTThe solution properties of two different glycoforms of IgG1 (IgG1Cri and IgG1Wid) are compared using primarily sedimentation equilibrium analysis with two complementary analysis routines: SEDFIT-MSTAR and MULTISIG. IgGCri bears diantennary complex-type glycans on its Fc domain that are fully core fucosylated and partially sialylated, whilst on IgGWid, they are non-fucosylated, partially galactosylated and non-sialylated. IgGWid is also Fab glycosylated. Despite these differences, SEDFIT-MSTAR analysis shows similar weight average mol...
Source: European Biophysics Journal : EBJ - May 17, 2023 Category: Physics Authors: Khalil Abu Hammad Vlad Dinu Thomas E MacCalman Jacob Pattem Margaret Goodall Richard B Gillis Roy Jefferis Stephen E Harding Source Type: research
Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid
Eur Biophys J. 2023 May 17. doi: 10.1007/s00249-023-01656-x. Online ahead of print.ABSTRACTThe solution properties of two different glycoforms of IgG1 (IgG1Cri and IgG1Wid) are compared using primarily sedimentation equilibrium analysis with two complementary analysis routines: SEDFIT-MSTAR and MULTISIG. IgGCri bears diantennary complex-type glycans on its Fc domain that are fully core fucosylated and partially sialylated, whilst on IgGWid, they are non-fucosylated, partially galactosylated and non-sialylated. IgGWid is also Fab glycosylated. Despite these differences, SEDFIT-MSTAR analysis shows similar weight average mol...
Source: European Biophysics Journal : EBJ - May 17, 2023 Category: Physics Authors: Khalil Abu Hammad Vlad Dinu Thomas E MacCalman Jacob Pattem Margaret Goodall Richard B Gillis Roy Jefferis Stephen E Harding Source Type: research
Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid
Eur Biophys J. 2023 May 17. doi: 10.1007/s00249-023-01656-x. Online ahead of print.ABSTRACTThe solution properties of two different glycoforms of IgG1 (IgG1Cri and IgG1Wid) are compared using primarily sedimentation equilibrium analysis with two complementary analysis routines: SEDFIT-MSTAR and MULTISIG. IgGCri bears diantennary complex-type glycans on its Fc domain that are fully core fucosylated and partially sialylated, whilst on IgGWid, they are non-fucosylated, partially galactosylated and non-sialylated. IgGWid is also Fab glycosylated. Despite these differences, SEDFIT-MSTAR analysis shows similar weight average mol...
Source: European Biophysics Journal : EBJ - May 17, 2023 Category: Physics Authors: Khalil Abu Hammad Vlad Dinu Thomas E MacCalman Jacob Pattem Margaret Goodall Richard B Gillis Roy Jefferis Stephen E Harding Source Type: research
