Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
Eur Biophys J. 2023 Jun 8. doi: 10.1007/s00249-023-01663-y. Online ahead of print.ABSTRACTLipid nanoparticles as delivery system for mRNA have recently attracted attention to a broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to deliver a variety of nucleic acids renders them an interesting and complementary alternative to gene therapy vectors like AAVs. An important quality attribute of LNPs is the copy number of the encapsulated cargo molecule. This work describes how density and molecular weight distributions obtained by density contrast sedimentation velocity can be used to calculate ...
Source: European Biophysics Journal : EBJ - June 8, 2023 Category: Physics Authors: Alexander Bepperling Gesa Richter Source Type: research
Pulling short DNA with mismatch base pairs
Eur Biophys J. 2023 May 30. doi: 10.1007/s00249-023-01659-8. Online ahead of print.ABSTRACTDue to misincorporation during gene replication, the accuracy of the gene expression is often compromised. This results in a mismatch or defective pair in the DNA molecule (James et al. 2016). Here, we present our study of the stability of DNA with defects in the thermal and force ensembles. We consider DNA with a different number of defects from 2to16 and study how the denaturation process differs in both ensembles. Using a statistical model, we calculate the melting point of the DNA chain in both the ensemble. Our findings display ...
Source: European Biophysics Journal : EBJ - May 30, 2023 Category: Physics Authors: Navin Singh Nehal Mathur Source Type: research
Pulling short DNA with mismatch base pairs
Eur Biophys J. 2023 May 30. doi: 10.1007/s00249-023-01659-8. Online ahead of print.ABSTRACTDue to misincorporation during gene replication, the accuracy of the gene expression is often compromised. This results in a mismatch or defective pair in the DNA molecule (James et al. 2016). Here, we present our study of the stability of DNA with defects in the thermal and force ensembles. We consider DNA with a different number of defects from 2to16 and study how the denaturation process differs in both ensembles. Using a statistical model, we calculate the melting point of the DNA chain in both the ensemble. Our findings display ...
Source: European Biophysics Journal : EBJ - May 30, 2023 Category: Physics Authors: Navin Singh Nehal Mathur Source Type: research
Pulling short DNA with mismatch base pairs
Eur Biophys J. 2023 May 30. doi: 10.1007/s00249-023-01659-8. Online ahead of print.ABSTRACTDue to misincorporation during gene replication, the accuracy of the gene expression is often compromised. This results in a mismatch or defective pair in the DNA molecule (James et al. 2016). Here, we present our study of the stability of DNA with defects in the thermal and force ensembles. We consider DNA with a different number of defects from 2to16 and study how the denaturation process differs in both ensembles. Using a statistical model, we calculate the melting point of the DNA chain in both the ensemble. Our findings display ...
Source: European Biophysics Journal : EBJ - May 30, 2023 Category: Physics Authors: Navin Singh Nehal Mathur Source Type: research
Pulling short DNA with mismatch base pairs
Eur Biophys J. 2023 May 30. doi: 10.1007/s00249-023-01659-8. Online ahead of print.ABSTRACTDue to misincorporation during gene replication, the accuracy of the gene expression is often compromised. This results in a mismatch or defective pair in the DNA molecule (James et al. 2016). Here, we present our study of the stability of DNA with defects in the thermal and force ensembles. We consider DNA with a different number of defects from 2to16 and study how the denaturation process differs in both ensembles. Using a statistical model, we calculate the melting point of the DNA chain in both the ensemble. Our findings display ...
Source: European Biophysics Journal : EBJ - May 30, 2023 Category: Physics Authors: Navin Singh Nehal Mathur Source Type: research
Pulling short DNA with mismatch base pairs
Eur Biophys J. 2023 May 30. doi: 10.1007/s00249-023-01659-8. Online ahead of print.ABSTRACTDue to misincorporation during gene replication, the accuracy of the gene expression is often compromised. This results in a mismatch or defective pair in the DNA molecule (James et al. 2016). Here, we present our study of the stability of DNA with defects in the thermal and force ensembles. We consider DNA with a different number of defects from 2to16 and study how the denaturation process differs in both ensembles. Using a statistical model, we calculate the melting point of the DNA chain in both the ensemble. Our findings display ...
Source: European Biophysics Journal : EBJ - May 30, 2023 Category: Physics Authors: Navin Singh Nehal Mathur Source Type: research
Pulling short DNA with mismatch base pairs
Eur Biophys J. 2023 May 30. doi: 10.1007/s00249-023-01659-8. Online ahead of print.ABSTRACTDue to misincorporation during gene replication, the accuracy of the gene expression is often compromised. This results in a mismatch or defective pair in the DNA molecule (James et al. 2016). Here, we present our study of the stability of DNA with defects in the thermal and force ensembles. We consider DNA with a different number of defects from 2to16 and study how the denaturation process differs in both ensembles. Using a statistical model, we calculate the melting point of the DNA chain in both the ensemble. Our findings display ...
Source: European Biophysics Journal : EBJ - May 30, 2023 Category: Physics Authors: Navin Singh Nehal Mathur Source Type: research
Comparison of three AUC techniques for the determination of the loading status and capsid titer of AAVs
Eur Biophys J. 2023 May 28. doi: 10.1007/s00249-023-01661-0. Online ahead of print.ABSTRACTDue to the rise of adeno-associated viruses (AAVs) as gene therapy delivery vectors, boundary sedimentation velocity analytical ultracentrifugation (boundary SV-AUC) has been developed into a widely used quality control assay even for release analytics. It can be considered as the "gold standard" for the determination of the loading status of empty, partially filled, and full capsids especially when conducted in multiwavelength (MWL) mode. It can be considered to provide the most accurate determination of the loading status, and it a...
Source: European Biophysics Journal : EBJ - May 28, 2023 Category: Physics Authors: Alexander Bepperling Janine Best Source Type: research
Comparison of three AUC techniques for the determination of the loading status and capsid titer of AAVs
Eur Biophys J. 2023 May 28. doi: 10.1007/s00249-023-01661-0. Online ahead of print.ABSTRACTDue to the rise of adeno-associated viruses (AAVs) as gene therapy delivery vectors, boundary sedimentation velocity analytical ultracentrifugation (boundary SV-AUC) has been developed into a widely used quality control assay even for release analytics. It can be considered as the "gold standard" for the determination of the loading status of empty, partially filled, and full capsids especially when conducted in multiwavelength (MWL) mode. It can be considered to provide the most accurate determination of the loading status, and it a...
Source: European Biophysics Journal : EBJ - May 28, 2023 Category: Physics Authors: Alexander Bepperling Janine Best Source Type: research
Mechanistic insights of neuronal calcium and IP < sub > 3 < /sub > signaling system regulating ATP release during ischemia in progression of Alzheimer's disease
Eur Biophys J. 2023 May 24. doi: 10.1007/s00249-023-01660-1. Online ahead of print.ABSTRACTThe mechanisms of calcium ([Ca2+]) signaling in various human cells have been widely analyzed by scientists due to its crucial role in human organs like the heartbeat, muscle contractions, bone activity, brain functionality, etc. No study is reported for interdependent [Ca2+] and IP3 mechanics regulating the release of ATP in neuron cells during Ischemia in Alzheimer's disease advancement. In the present investigation, a finite element method (FEM) is framed to explore the interdependence of spatiotemporal [Ca2+] and IP3 signaling me...
Source: European Biophysics Journal : EBJ - May 24, 2023 Category: Physics Authors: Anand Pawar Kamal Raj Pardasani Source Type: research
Mechanistic insights of neuronal calcium and IP < sub > 3 < /sub > signaling system regulating ATP release during ischemia in progression of Alzheimer's disease
Eur Biophys J. 2023 May 24. doi: 10.1007/s00249-023-01660-1. Online ahead of print.ABSTRACTThe mechanisms of calcium ([Ca2+]) signaling in various human cells have been widely analyzed by scientists due to its crucial role in human organs like the heartbeat, muscle contractions, bone activity, brain functionality, etc. No study is reported for interdependent [Ca2+] and IP3 mechanics regulating the release of ATP in neuron cells during Ischemia in Alzheimer's disease advancement. In the present investigation, a finite element method (FEM) is framed to explore the interdependence of spatiotemporal [Ca2+] and IP3 signaling me...
Source: European Biophysics Journal : EBJ - May 24, 2023 Category: Physics Authors: Anand Pawar Kamal Raj Pardasani Source Type: research
Mechanistic insights of neuronal calcium and IP < sub > 3 < /sub > signaling system regulating ATP release during ischemia in progression of Alzheimer's disease
Eur Biophys J. 2023 May 24. doi: 10.1007/s00249-023-01660-1. Online ahead of print.ABSTRACTThe mechanisms of calcium ([Ca2+]) signaling in various human cells have been widely analyzed by scientists due to its crucial role in human organs like the heartbeat, muscle contractions, bone activity, brain functionality, etc. No study is reported for interdependent [Ca2+] and IP3 mechanics regulating the release of ATP in neuron cells during Ischemia in Alzheimer's disease advancement. In the present investigation, a finite element method (FEM) is framed to explore the interdependence of spatiotemporal [Ca2+] and IP3 signaling me...
Source: European Biophysics Journal : EBJ - May 24, 2023 Category: Physics Authors: Anand Pawar Kamal Raj Pardasani Source Type: research
Mechanistic insights of neuronal calcium and IP < sub > 3 < /sub > signaling system regulating ATP release during ischemia in progression of Alzheimer's disease
Eur Biophys J. 2023 May 24. doi: 10.1007/s00249-023-01660-1. Online ahead of print.ABSTRACTThe mechanisms of calcium ([Ca2+]) signaling in various human cells have been widely analyzed by scientists due to its crucial role in human organs like the heartbeat, muscle contractions, bone activity, brain functionality, etc. No study is reported for interdependent [Ca2+] and IP3 mechanics regulating the release of ATP in neuron cells during Ischemia in Alzheimer's disease advancement. In the present investigation, a finite element method (FEM) is framed to explore the interdependence of spatiotemporal [Ca2+] and IP3 signaling me...
Source: European Biophysics Journal : EBJ - May 24, 2023 Category: Physics Authors: Anand Pawar Kamal Raj Pardasani Source Type: research
SDS-induced hexameric oligomerization of myotoxin-II from Bothrops asper assessed by sedimentation velocity and nuclear magnetic resonance
We report the solution behavior, oligomerization state, and structural details of myotoxin-II purified from the venom of Bothrops asper in the presence and absence of sodium dodecyl sulfate (SDS) and multiple lipids, as examined by analytical ultracentrifugation and nuclear magnetic resonance. Molecular functional and structural details of the myotoxic mechanism of group II Lys-49 phospholipase A2 homologues have been only partially elucidated so far, and conflicting observations have been reported in the literature regarding the monomeric vs. oligomeric state of these toxins in solution. We observed the formation of a sta...
Source: European Biophysics Journal : EBJ - May 20, 2023 Category: Physics Authors: Amy Henrickson Tony Montina Paul Hazendonk Bruno Lomonte Ana Gisele C Neves-Ferreira Borries Demeler Source Type: research
SDS-induced hexameric oligomerization of myotoxin-II from Bothrops asper assessed by sedimentation velocity and nuclear magnetic resonance
We report the solution behavior, oligomerization state, and structural details of myotoxin-II purified from the venom of Bothrops asper in the presence and absence of sodium dodecyl sulfate (SDS) and multiple lipids, as examined by analytical ultracentrifugation and nuclear magnetic resonance. Molecular functional and structural details of the myotoxic mechanism of group II Lys-49 phospholipase A2 homologues have been only partially elucidated so far, and conflicting observations have been reported in the literature regarding the monomeric vs. oligomeric state of these toxins in solution. We observed the formation of a sta...
Source: European Biophysics Journal : EBJ - May 20, 2023 Category: Physics Authors: Amy Henrickson Tony Montina Paul Hazendonk Bruno Lomonte Ana Gisele C Neves-Ferreira Borries Demeler Source Type: research
