Mask exhibits trxG-like behavior and associates with H3K27ac marked chromatin
Dev Biol. 2023 Nov 17:S0012-1606(23)00188-4. doi: 10.1016/j.ydbio.2023.11.005. Online ahead of print.ABSTRACTThe Trithorax group (trxG) proteins counteract the repressive effect of Polycomb group (PcG) complexes and maintain transcriptional memory of active states of key developmental genes. Although chromatin structure and modifications appear to play a fundamental role in this process, it is not clear how trxG prevents PcG-silencing and heritably maintains an active gene expression state. Here, we report a hitherto unknown role of Drosophila Multiple ankyrin repeats single KH domain (Mask), which emerged as one of the ca...
Source: Developmental Biology - November 19, 2023 Category: Biology Authors: Ammad Shaukat Mahnoor Hussain Bakhtiari Daim Shiraz Chaudhry Muhammad Haider Farooq Khan Jawad Akhtar Ahmed Hassan Abro Muhammad Abdul Haseeb Aaminah Sarwar Khalida Mazhar Zain Umer Muhammad Tariq Source Type: research
The ftz upstream element drives late ftz stripes but is not required for regulation of ftz target genes
Dev Biol. 2023 Nov 15:S0012-1606(23)00187-2. doi: 10.1016/j.ydbio.2023.11.004. Online ahead of print.ABSTRACTThe regulation of gene expression in precise, rapidly changing spatial patterns is essential for embryonic development. Multiple enhancers have been identified for the evolving expression patterns of the cascade of Drosophila segmentation genes that establish the basic body plan of the fly. Classic reporter transgene experiments identified multiple cis-regulatory elements (CREs) that are sufficient to direct various aspects of the evolving expression pattern of the pair-rule gene fushi tarazu (ftz). These include en...
Source: Developmental Biology - November 17, 2023 Category: Biology Authors: Mathew D Fischera Patricia Graham Leslie Pick Source Type: research
The ftz upstream element drives late ftz stripes but is not required for regulation of ftz target genes
Dev Biol. 2023 Nov 15:S0012-1606(23)00187-2. doi: 10.1016/j.ydbio.2023.11.004. Online ahead of print.ABSTRACTThe regulation of gene expression in precise, rapidly changing spatial patterns is essential for embryonic development. Multiple enhancers have been identified for the evolving expression patterns of the cascade of Drosophila segmentation genes that establish the basic body plan of the fly. Classic reporter transgene experiments identified multiple cis-regulatory elements (CREs) that are sufficient to direct various aspects of the evolving expression pattern of the pair-rule gene fushi tarazu (ftz). These include en...
Source: Developmental Biology - November 17, 2023 Category: Biology Authors: Mathew D Fischera Patricia Graham Leslie Pick Source Type: research
The ftz upstream element drives late ftz stripes but is not required for regulation of ftz target genes
Dev Biol. 2023 Nov 15:S0012-1606(23)00187-2. doi: 10.1016/j.ydbio.2023.11.004. Online ahead of print.ABSTRACTThe regulation of gene expression in precise, rapidly changing spatial patterns is essential for embryonic development. Multiple enhancers have been identified for the evolving expression patterns of the cascade of Drosophila segmentation genes that establish the basic body plan of the fly. Classic reporter transgene experiments identified multiple cis-regulatory elements (CREs) that are sufficient to direct various aspects of the evolving expression pattern of the pair-rule gene fushi tarazu (ftz). These include en...
Source: Developmental Biology - November 17, 2023 Category: Biology Authors: Mathew D Fischera Patricia Graham Leslie Pick Source Type: research
Islet architecture in adult mice is actively maintained by Robo2 expression in β cells
Dev Biol. 2023 Nov 14:S0012-1606(23)00186-0. doi: 10.1016/j.ydbio.2023.11.003. Online ahead of print.ABSTRACTA fundamental question in developmental biology is whether tissue architectures formed during development are set for life, or require continuous maintenance signals, and if so, what are those signals. The islets of Langerhans in the pancreas can serve as an elegant model tissue to answer these questions. Islets have a non-random spatial architecture, which is important to proper glucose homeostasis. Islet architecture forms during embryonic development, in a morphogenesis process partially involving expression of R...
Source: Developmental Biology - November 16, 2023 Category: Biology Authors: Bayley J Waters Zoe R Birman Matthew R Wagner Julia Lemanski Barak Blum Source Type: research
Myosin XV is a negative regulator of signaling filopodia during long-range lateral inhibition
Dev Biol. 2023 Nov 11:S0012-1606(23)00185-9. doi: 10.1016/j.ydbio.2023.11.002. Online ahead of print.ABSTRACTThe self-organization of cells during development is essential for the formation of healthy tissues and requires the coordination of cell activities at local scales. Cytonemes, or signaling filopodia, are dynamic actin-based cellular protrusions that allow cells to engage in contact mediated signaling at a distance. While signaling filopodia have been shown to support several signaling paradigms during development, less is understood about how these protrusions are regulated. We investigated the role of the plus-end...
Source: Developmental Biology - November 13, 2023 Category: Biology Authors: Rhiannon Clements Tyler Smith Luke Cowart Jennifer Zhumi Alan Sherrod Aidan Cahill Ginger L Hunter Source Type: research
Myosin XV is a negative regulator of signaling filopodia during long-range lateral inhibition
Dev Biol. 2023 Nov 11:S0012-1606(23)00185-9. doi: 10.1016/j.ydbio.2023.11.002. Online ahead of print.ABSTRACTThe self-organization of cells during development is essential for the formation of healthy tissues and requires the coordination of cell activities at local scales. Cytonemes, or signaling filopodia, are dynamic actin-based cellular protrusions that allow cells to engage in contact mediated signaling at a distance. While signaling filopodia have been shown to support several signaling paradigms during development, less is understood about how these protrusions are regulated. We investigated the role of the plus-end...
Source: Developmental Biology - November 13, 2023 Category: Biology Authors: Rhiannon Clements Tyler Smith Luke Cowart Jennifer Zhumi Alan Sherrod Aidan Cahill Ginger L Hunter Source Type: research
Myosin XV is a negative regulator of signaling filopodia during long-range lateral inhibition
Dev Biol. 2023 Nov 11:S0012-1606(23)00185-9. doi: 10.1016/j.ydbio.2023.11.002. Online ahead of print.ABSTRACTThe self-organization of cells during development is essential for the formation of healthy tissues and requires the coordination of cell activities at local scales. Cytonemes, or signaling filopodia, are dynamic actin-based cellular protrusions that allow cells to engage in contact mediated signaling at a distance. While signaling filopodia have been shown to support several signaling paradigms during development, less is understood about how these protrusions are regulated. We investigated the role of the plus-end...
Source: Developmental Biology - November 13, 2023 Category: Biology Authors: Rhiannon Clements Tyler Smith Luke Cowart Jennifer Zhumi Alan Sherrod Aidan Cahill Ginger L Hunter Source Type: research
Myosin XV is a negative regulator of signaling filopodia during long-range lateral inhibition
Dev Biol. 2023 Nov 11:S0012-1606(23)00185-9. doi: 10.1016/j.ydbio.2023.11.002. Online ahead of print.ABSTRACTThe self-organization of cells during development is essential for the formation of healthy tissues and requires the coordination of cell activities at local scales. Cytonemes, or signaling filopodia, are dynamic actin-based cellular protrusions that allow cells to engage in contact mediated signaling at a distance. While signaling filopodia have been shown to support several signaling paradigms during development, less is understood about how these protrusions are regulated. We investigated the role of the plus-end...
Source: Developmental Biology - November 13, 2023 Category: Biology Authors: Rhiannon Clements Tyler Smith Luke Cowart Jennifer Zhumi Alan Sherrod Aidan Cahill Ginger L Hunter Source Type: research
Experimental validation and characterization of putative targets of Escargot and STAT, two master regulators of the intestinal stem cells in Drosophila melanogaster
Dev Biol. 2023 Nov 10:S0012-1606(23)00176-8. doi: 10.1016/j.ydbio.2023.10.008. Online ahead of print.ABSTRACTMany organs contain adult stem cells (ASCs) to replace cells due to damage, disease, or normal tissue turnover. ASCs can divide asymmetrically, giving rise to a new copy of themselves (self-renewal) and a sister that commits to a specific cell type (differentiation). Decades of research have led to the identification of pleiotropic genes whose loss or gain of function affect diverse aspects of normal ASC biology. Genome-wide screens of these so-called genetic "master regulator" (MR) genes, have pointed to hundreds o...
Source: Developmental Biology - November 12, 2023 Category: Biology Authors: Armen Khanbabei Lina Segura Cynthia Petrossian Aaron Lemus Ithan Cano Courtney Frazier Armen Halajyan Donnie Ca Mariano Loza-Coll Source Type: research
Neurog1 and Olig2 integrate patterning and neurogenesis signals in development of zebrafish dopaminergic and glutamatergic dual transmitter neurons
Dev Biol. 2023 Nov 7;505:85-98. doi: 10.1016/j.ydbio.2023.10.005. Online ahead of print.ABSTRACTDopaminergic neurons develop in distinct neural domains by integrating local patterning and neurogenesis signals. While the proneural proteins Neurog1 and Olig2 have been previously linked to development of dopaminergic neurons, their dependence on local prepatterning and specific contributions to dopaminergic neurogenesis are not well understood. Here, we show that both transcription factors are differentially required for the development of defined dopaminergic glutamatergic subpopulations in the zebrafish posterior tuberculum...
Source: Developmental Biology - November 9, 2023 Category: Biology Authors: Christian Altb ürger Meta Rath Daniel Armbruster Wolfgang Driever Source Type: research
Neurog1 and Olig2 integrate patterning and neurogenesis signals in development of zebrafish dopaminergic and glutamatergic dual transmitter neurons
Dev Biol. 2023 Nov 7;505:85-98. doi: 10.1016/j.ydbio.2023.10.005. Online ahead of print.ABSTRACTDopaminergic neurons develop in distinct neural domains by integrating local patterning and neurogenesis signals. While the proneural proteins Neurog1 and Olig2 have been previously linked to development of dopaminergic neurons, their dependence on local prepatterning and specific contributions to dopaminergic neurogenesis are not well understood. Here, we show that both transcription factors are differentially required for the development of defined dopaminergic glutamatergic subpopulations in the zebrafish posterior tuberculum...
Source: Developmental Biology - November 9, 2023 Category: Biology Authors: Christian Altb ürger Meta Rath Daniel Armbruster Wolfgang Driever Source Type: research
Neurog1 and Olig2 integrate patterning and neurogenesis signals in development of zebrafish dopaminergic and glutamatergic dual transmitter neurons
Dev Biol. 2023 Nov 7;505:85-98. doi: 10.1016/j.ydbio.2023.10.005. Online ahead of print.ABSTRACTDopaminergic neurons develop in distinct neural domains by integrating local patterning and neurogenesis signals. While the proneural proteins Neurog1 and Olig2 have been previously linked to development of dopaminergic neurons, their dependence on local prepatterning and specific contributions to dopaminergic neurogenesis are not well understood. Here, we show that both transcription factors are differentially required for the development of defined dopaminergic glutamatergic subpopulations in the zebrafish posterior tuberculum...
Source: Developmental Biology - November 9, 2023 Category: Biology Authors: Christian Altb ürger Meta Rath Daniel Armbruster Wolfgang Driever Source Type: research
The proneural factors Ascl1a and Ascl1b contribute to the terminal differentiation of dopaminergic GABAergic dual transmitter neurons in zebrafish
Dev Biol. 2023 Nov 4;505:58-74. doi: 10.1016/j.ydbio.2023.10.006. Online ahead of print.ABSTRACTThe proneural factor Ascl1 is involved in several steps of neurogenesis, from neural progenitor maintenance to initiation of terminal differentiation and neuronal subtype specification. In neural progenitor cells, Ascl1 initiates the cell-cycle exit of progenitors, and contributes to their differentiation into mainly GABAergic neurons. Several catecholaminergic neuron groups in the forebrain of zebrafish use GABA as co-transmitter, but a potential role of the two paralogues Ascl1a and Ascl1b in their neurogenesis is not understo...
Source: Developmental Biology - November 6, 2023 Category: Biology Authors: Christian Altb ürger Meta Rath Johanna Wehrle Wolfgang Driever Source Type: research
The proneural factors Ascl1a and Ascl1b contribute to the terminal differentiation of dopaminergic GABAergic dual transmitter neurons in zebrafish
Dev Biol. 2023 Nov 4;505:58-74. doi: 10.1016/j.ydbio.2023.10.006. Online ahead of print.ABSTRACTThe proneural factor Ascl1 is involved in several steps of neurogenesis, from neural progenitor maintenance to initiation of terminal differentiation and neuronal subtype specification. In neural progenitor cells, Ascl1 initiates the cell-cycle exit of progenitors, and contributes to their differentiation into mainly GABAergic neurons. Several catecholaminergic neuron groups in the forebrain of zebrafish use GABA as co-transmitter, but a potential role of the two paralogues Ascl1a and Ascl1b in their neurogenesis is not understo...
Source: Developmental Biology - November 6, 2023 Category: Biology Authors: Christian Altb ürger Meta Rath Johanna Wehrle Wolfgang Driever Source Type: research
