Corrigendum to "A single-cell RNA-seq analysis of early larval cell-types of the starfish, Patiria pectinifera: Insights into evolution of the chordate body plan" [Dev. Biol. 496 (2023) 52-62]
Dev Biol. 2023 Jul 14;502:38. doi: 10.1016/j.ydbio.2023.06.014. Online ahead of print.NO ABSTRACTPMID:37454579 | DOI:10.1016/j.ydbio.2023.06.014 (Source: Developmental Biology)
Source: Developmental Biology - July 16, 2023 Category: Biology Authors: Hitoshi Tominaga Koki Nishitsuji Noriyuki Satoh Source Type: research
Corrigendum to "A single-cell RNA-seq analysis of early larval cell-types of the starfish, Patiria pectinifera: Insights into evolution of the chordate body plan" [Dev. Biol. 496 (2023) 52-62]
Dev Biol. 2023 Jul 14;502:38. doi: 10.1016/j.ydbio.2023.06.014. Online ahead of print.NO ABSTRACTPMID:37454579 | DOI:10.1016/j.ydbio.2023.06.014 (Source: Developmental Biology)
Source: Developmental Biology - July 16, 2023 Category: Biology Authors: Hitoshi Tominaga Koki Nishitsuji Noriyuki Satoh Source Type: research
Corrigendum to "A single-cell RNA-seq analysis of early larval cell-types of the starfish, Patiria pectinifera: Insights into evolution of the chordate body plan" [Dev. Biol. 496 (2023) 52-62]
Dev Biol. 2023 Jul 14;502:38. doi: 10.1016/j.ydbio.2023.06.014. Online ahead of print.NO ABSTRACTPMID:37454579 | DOI:10.1016/j.ydbio.2023.06.014 (Source: Developmental Biology)
Source: Developmental Biology - July 16, 2023 Category: Biology Authors: Hitoshi Tominaga Koki Nishitsuji Noriyuki Satoh Source Type: research
Corrigendum to "A single-cell RNA-seq analysis of early larval cell-types of the starfish, Patiria pectinifera: Insights into evolution of the chordate body plan" [Dev. Biol. 496 (2023) 52-62]
Dev Biol. 2023 Jul 14;502:38. doi: 10.1016/j.ydbio.2023.06.014. Online ahead of print.NO ABSTRACTPMID:37454579 | DOI:10.1016/j.ydbio.2023.06.014 (Source: Developmental Biology)
Source: Developmental Biology - July 16, 2023 Category: Biology Authors: Hitoshi Tominaga Koki Nishitsuji Noriyuki Satoh Source Type: research
Corrigendum to "A single-cell RNA-seq analysis of early larval cell-types of the starfish, Patiria pectinifera: Insights into evolution of the chordate body plan" [Dev. Biol. 496 (2023) 52-62]
Dev Biol. 2023 Jul 14;502:38. doi: 10.1016/j.ydbio.2023.06.014. Online ahead of print.NO ABSTRACTPMID:37454579 | DOI:10.1016/j.ydbio.2023.06.014 (Source: Developmental Biology)
Source: Developmental Biology - July 16, 2023 Category: Biology Authors: Hitoshi Tominaga Koki Nishitsuji Noriyuki Satoh Source Type: research
Integrative analysis of single-cell embryo data reveals transcriptome signatures for the human pre-implantation inner cell mass
Dev Biol. 2023 Jul 10:S0012-1606(23)00130-6. doi: 10.1016/j.ydbio.2023.07.004. Online ahead of print.ABSTRACTAs the source of embryonic stem cells (ESCs), inner cell mass (ICM) can form all tissues of the embryo proper, however, its role in early human lineage specification remains controversial. Although a stepwise differentiation model has been proposed suggesting the existence of ICM as a distinct developmental stage, the underlying molecular mechanism remains unclear. In the present study, we perform an integrated analysis on the public human preimplantation embryonic single-cell transcriptomic data and apply a traject...
Source: Developmental Biology - July 12, 2023 Category: Biology Authors: Xinshu Wei Xiang Fang Xiu Yu Hong Li Yuyang Guo Yifei Qi Chuanbo Sun Dingding Han Xiaonan Liu Na Li Hao Hu Source Type: research
Integrative analysis of single-cell embryo data reveals transcriptome signatures for the human pre-implantation inner cell mass
Dev Biol. 2023 Jul 10:S0012-1606(23)00130-6. doi: 10.1016/j.ydbio.2023.07.004. Online ahead of print.ABSTRACTAs the source of embryonic stem cells (ESCs), inner cell mass (ICM) can form all tissues of the embryo proper, however, its role in early human lineage specification remains controversial. Although a stepwise differentiation model has been proposed suggesting the existence of ICM as a distinct developmental stage, the underlying molecular mechanism remains unclear. In the present study, we perform an integrated analysis on the public human preimplantation embryonic single-cell transcriptomic data and apply a traject...
Source: Developmental Biology - July 12, 2023 Category: Biology Authors: Xinshu Wei Xiang Fang Xiu Yu Hong Li Yuyang Guo Yifei Qi Chuanbo Sun Dingding Han Xiaonan Liu Na Li Hao Hu Source Type: research
Integrative analysis of single-cell embryo data reveals transcriptome signatures for the human pre-implantation inner cell mass
Dev Biol. 2023 Jul 10:S0012-1606(23)00130-6. doi: 10.1016/j.ydbio.2023.07.004. Online ahead of print.ABSTRACTAs the source of embryonic stem cells (ESCs), inner cell mass (ICM) can form all tissues of the embryo proper, however, its role in early human lineage specification remains controversial. Although a stepwise differentiation model has been proposed suggesting the existence of ICM as a distinct developmental stage, the underlying molecular mechanism remains unclear. In the present study, we perform an integrated analysis on the public human preimplantation embryonic single-cell transcriptomic data and apply a traject...
Source: Developmental Biology - July 12, 2023 Category: Biology Authors: Xinshu Wei Xiang Fang Xiu Yu Hong Li Yuyang Guo Yifei Qi Chuanbo Sun Dingding Han Xiaonan Liu Na Li Hao Hu Source Type: research
Integrative analysis of single-cell embryo data reveals transcriptome signatures for the human pre-implantation inner cell mass
Dev Biol. 2023 Jul 10:S0012-1606(23)00130-6. doi: 10.1016/j.ydbio.2023.07.004. Online ahead of print.ABSTRACTAs the source of embryonic stem cells (ESCs), inner cell mass (ICM) can form all tissues of the embryo proper, however, its role in early human lineage specification remains controversial. Although a stepwise differentiation model has been proposed suggesting the existence of ICM as a distinct developmental stage, the underlying molecular mechanism remains unclear. In the present study, we perform an integrated analysis on the public human preimplantation embryonic single-cell transcriptomic data and apply a traject...
Source: Developmental Biology - July 12, 2023 Category: Biology Authors: Xinshu Wei Xiang Fang Xiu Yu Hong Li Yuyang Guo Yifei Qi Chuanbo Sun Dingding Han Xiaonan Liu Na Li Hao Hu Source Type: research
A simple method to dramatically increase C. elegans germline microinjection efficiency
Dev Biol. 2023 Jul 9:S0012-1606(23)00129-X. doi: 10.1016/j.ydbio.2023.07.003. Online ahead of print.ABSTRACTGenome manipulation methods in C. elegans require microinjecting DNA or ribonucleoprotein complexes into the microscopic core of the gonadal syncytium. These microinjections are technically demanding and represent a key bottleneck for all genome engineering and transgenic approaches in C. elegans. While there have been steady improvements in the ease and efficiency of genetic methods for C. elegans genome manipulation, there have not been comparable advances in the physical process of microinjection. Here, we report ...
Source: Developmental Biology - July 11, 2023 Category: Biology Authors: Theresa V Gibney Michelle Favichia Laila Latifi Taylor N Medwig-Kinney David Q Matus Daniel C McIntyre Angelo B Arrigo Kendall R Branham Louis T Bubrig Abbas Ghaddar Juliana A Jiranek Kendra E Liu Charles G Marcucci Robert J Porter Ariel M Pani Source Type: research
Transcriptional network governing extraembryonic endoderm cell fate choice
Dev Biol. 2023 Jul 7:S0012-1606(23)00128-8. doi: 10.1016/j.ydbio.2023.07.002. Online ahead of print.ABSTRACTThe mechanism by which transcription factor (TF) network instructs cell-type-specific transcriptional programs to drive primitive endoderm (PrE) progenitors to commit to parietal endoderm (PE) versus visceral endoderm (VE) cell fates remains poorly understood. To address the question, we analyzed the single-cell transcriptional signatures defining PrE, PE, and VE cell states during the onset of the PE-VE lineage bifurcation. By coupling with the epigenomic comparison of active enhancers unique to PE and VE cells, we ...
Source: Developmental Biology - July 9, 2023 Category: Biology Authors: Paula Duyen Pham Hanbin Lu Han Han Jeff Jiajing Zhou Aarushi Madan Wenqi Wang Cornelis Murre Ken W Y Cho Source Type: research
Transcriptional network governing extraembryonic endoderm cell fate choice
Dev Biol. 2023 Jul 7:S0012-1606(23)00128-8. doi: 10.1016/j.ydbio.2023.07.002. Online ahead of print.ABSTRACTThe mechanism by which transcription factor (TF) network instructs cell-type-specific transcriptional programs to drive primitive endoderm (PrE) progenitors to commit to parietal endoderm (PE) versus visceral endoderm (VE) cell fates remains poorly understood. To address the question, we analyzed the single-cell transcriptional signatures defining PrE, PE, and VE cell states during the onset of the PE-VE lineage bifurcation. By coupling with the epigenomic comparison of active enhancers unique to PE and VE cells, we ...
Source: Developmental Biology - July 9, 2023 Category: Biology Authors: Paula Duyen Pham Hanbin Lu Han Han Jeff Jiajing Zhou Aarushi Madan Wenqi Wang Cornelis Murre Ken W Y Cho Source Type: research
Transcriptional network governing extraembryonic endoderm cell fate choice
Dev Biol. 2023 Jul 7:S0012-1606(23)00128-8. doi: 10.1016/j.ydbio.2023.07.002. Online ahead of print.ABSTRACTThe mechanism by which transcription factor (TF) network instructs cell-type-specific transcriptional programs to drive primitive endoderm (PrE) progenitors to commit to parietal endoderm (PE) versus visceral endoderm (VE) cell fates remains poorly understood. To address the question, we analyzed the single-cell transcriptional signatures defining PrE, PE, and VE cell states during the onset of the PE-VE lineage bifurcation. By coupling with the epigenomic comparison of active enhancers unique to PE and VE cells, we ...
Source: Developmental Biology - July 9, 2023 Category: Biology Authors: Paula Duyen Pham Hanbin Lu Han Han Jeff Jiajing Zhou Aarushi Madan Wenqi Wang Cornelis Murre Ken W Y Cho Source Type: research
In revision microRNA-124 directly suppresses Nodal and Notch to regulate mesodermal development
This study discovers a novel role of miR-124 in regulating mesodermal cell differentiation in the sea urchin embryo. The expression of miR-124 is first detectable at 12 h post fertilization at the early blastula stage, during endomesodermal specification. Mesodermally-derived immune cells come from the same progenitor cells that give rise to blastocoelar cells (BCs) and pigment cells (PCs) that must make a binary fate decision. We determined that miR-124 directly represses Nodal and Notch to regulate BC and PC differentiation. miR-124 inhibition does not impact the dorsal-ventral axis formation, but result in a significant...
Source: Developmental Biology - July 7, 2023 Category: Biology Authors: Kalin D Konrad Malcolm Arnott Michael Testa Santiago Suarez Jia L Song Source Type: research
In revision microRNA-124 directly suppresses Nodal and Notch to regulate mesodermal development
This study discovers a novel role of miR-124 in regulating mesodermal cell differentiation in the sea urchin embryo. The expression of miR-124 is first detectable at 12 h post fertilization at the early blastula stage, during endomesodermal specification. Mesodermally-derived immune cells come from the same progenitor cells that give rise to blastocoelar cells (BCs) and pigment cells (PCs) that must make a binary fate decision. We determined that miR-124 directly represses Nodal and Notch to regulate BC and PC differentiation. miR-124 inhibition does not impact the dorsal-ventral axis formation, but result in a significant...
Source: Developmental Biology - July 7, 2023 Category: Biology Authors: Kalin D Konrad Malcolm Arnott Michael Testa Santiago Suarez Jia L Song Source Type: research
