Distinct developmental patterns in Anopheles stephensi organ systems
This study presents a comprehensive, high-resolution developmental profile of Anopheles stephensi, encompassing larval, pupal, and adult stages, obtained through microCT scanning. The results indicate in situ anatomical changes in most organ systems, including the central nervous system, eyes, musculature, alimentary canal, salivary glands, and ovaries, among other organ systems, except for the developing heart. We find significant differences in the mosquito gut, body-wall, and flight muscle development during metamorphosis from other dipterans like Drosophila. Specifically, indirect flight muscle specification and growth...
Source: Developmental Biology - January 25, 2024 Category: Biology Authors: Khushboo Agrawal Sunil Prabhakar Baskar Bakthavachalu Dhananjay Chaturvedi Source Type: research
ARF6, a component of intercellular bridges, is essential for spermatogenesis in mice
Dev Biol. 2024 Jan 17;508:46-63. doi: 10.1016/j.ydbio.2024.01.007. Online ahead of print.ABSTRACTMale germ cells are connected by intercellular bridges (ICBs) in a syncytium due to incomplete cytokinesis. Syncytium is thought to be important for synchronized germ cell development by interchange of cytoplasmic factors via ICBs. Mammalian ADP-ribosylation factor 6 (ARF6) is a small GTPase that is involved in many cellular mechanisms including but not limited to regulating cellular structure, motility, vesicle trafficking and cytokinesis. ARF6 localizes to ICBs in spermatogonia and spermatocytes in mice. Here we report that m...
Source: Developmental Biology - January 19, 2024 Category: Biology Authors: Hetty N Wong Tingfang Chen P Jeremy Wang Lawrence B Holzman Source Type: research
Neuronal excitability modulates developmental time of Drosophila melanogaster
Dev Biol. 2024 Jan 14;508:38-45. doi: 10.1016/j.ydbio.2024.01.006. Online ahead of print.ABSTRACTDevelopmental time is a fundamental life history trait that affects the reproductive success of animals. Developmental time is known to be regulated by many genes and environmental conditions, yet mechanistic understandings of how various cellular processes influence the developmental timing of an organism are lacking. The nervous system is known to control key processes that affect developmental time, including the release of hormones that signal transitions between developmental stages. Here we show that the excitability of n...
Source: Developmental Biology - January 15, 2024 Category: Biology Authors: Aidan P C Dermady Dionna L DeFazio Emily M Hensley Daniel L Ruiz Alejandra D Chavez Sarah A Iannone Niall M Dermady Lexis V Grandel Alexis S Hill Source Type: research
scRNA-seq analysis of cells comprising the amphioxus notochord
We examined these properties using Iso-seq analysis, single-cell RNA-seq analysis, and in situ hybridization (ISH). Gene expression profiles broadly categorize notochordal cells into myofibrillar cells and non-myofibrillar cells. Myofibrillar cells occupy most of the central portion of the notochord, and some cells extend the notochordal horn to both sides of the ventral nerve cord. Some notochord myofibrillar genes are not expressed in myotomes, suggesting an occurrence of myofibrillar genes that are preferentially expressed in notochord. On the other hand, non-myofibrillar cells contain dorsal, lateral, and ventral Müll...
Source: Developmental Biology - January 15, 2024 Category: Biology Authors: Hiroki Takahashi Kanako Hisata Rin Iguchi Sakura Kikuchi Michio Ogasawara Noriyuki Satoh Source Type: research
Measuring transcription factor function with cell type-specific somatic transgenesis in chicken embryos
Dev Biol. 2024 Jan 11;508:1-7. doi: 10.1016/j.ydbio.2024.01.005. Online ahead of print.ABSTRACTRetroviral-mediated misexpression in chicken embryos has been a powerful research tool for developmental biologists in the last two decades. In the RCASBP retroviral vectors that are widely used for in vivo somatic transgenesis, a coding sequence of interest is under the transcriptional control of a strong viral promoter in the long terminal repeat. While this has proven to be effective for studying secreted signalling proteins, interpretation of the mechanisms of action of nuclear factors is more difficult using this system sinc...
Source: Developmental Biology - January 13, 2024 Category: Biology Authors: Hui Zhu Andrew J Bendall Source Type: research
Fecundity is optimized by levels of nutrient signal-dependent expression of Dve and EcR in Drosophila male accessory gland
Dev Biol. 2024 Jan 8;508:8-23. doi: 10.1016/j.ydbio.2024.01.004. Online ahead of print.ABSTRACTSteroid hormones play various physiological roles including metabolism and reproduction. Steroid hormones in insects are ecdysteroids, and the major form in Drosophila melanogaster is ecdysone. In Drosophila males, the accessory gland is responsive to nutrient-dependent regulation of fertility/fecundity. The accessory gland is composed of two types of binucleated epithelial cells: a main cell and a secondary cell (SC). The transcription factors Defective proventriculus (Dve), Abdominal-B, and Ecdysone receptors (EcRs) are strongl...
Source: Developmental Biology - January 10, 2024 Category: Biology Authors: Mirai Matsuka Shinichi Otsune Seiko Sugimori Yasuhiro Tsugita Hitoshi Ueda Hideki Nakagoshi Source Type: research
The avian ectodermal default competence to make feathers
Dev Biol. 2024 Jan 6;508:64-76. doi: 10.1016/j.ydbio.2024.01.002. Online ahead of print.ABSTRACTFeathers originate as protofeathers before birds, in pterosaurs and basal dinosaurs. What characterizes a feather is not only its outgrowth, but its barb cells differentiation and a set of beta-corneous proteins. Reticula appear concomitantly with feathers, as small bumps on plantar skin, made only of keratins. Avian scales, with their own set of beta-corneous proteins, appear more recently than feathers on the shank, and only in some species. In the chick embryo, when feather placodes form, all the non-feather areas of the inte...
Source: Developmental Biology - January 8, 2024 Category: Biology Authors: Danielle Dhouailly Source Type: research
The avian ectodermal default competence to make feathers
Dev Biol. 2024 Jan 6:S0012-1606(24)00002-2. doi: 10.1016/j.ydbio.2024.01.002. Online ahead of print.ABSTRACTFeathers originate as protofeathers before birds, in pterosaurs and basal dinosaurs. What characterizes a feather is not only its outgrowth, but its barb cells differentiation and a set of beta-corneous proteins. Reticula appear concomitantly with feathers, as small bumps on plantar skin, made only of keratins. Avian scales, with their own set of beta-corneous proteins, appear more recently than feathers on the shank, and only in some species. In the chick embryo, when feather placodes form, all the non-feather areas...
Source: Developmental Biology - January 8, 2024 Category: Biology Authors: Danielle Dhouailly Source Type: research
Molecular aspects of regeneration in insects
This report highlights the major breakthrough research on wound healing and tissue regeneration. Exploring and reviewing the molecular basis of regeneration can be helpful in the area of regenerative medicine advancements. The understanding gathered from this framework can potentially contribute to hypothesis designing with implications in the field of synthetic biology and human health.PMID:38160963 | DOI:10.1016/j.ydbio.2023.12.011 (Source: Developmental Biology)
Source: Developmental Biology - December 31, 2023 Category: Biology Authors: Shivali Pandita Sanchita Singh Sanjay Kumar Bajpai Geetanjali Mishra Gauri Saxena Praveen C Verma Source Type: research
Molecular aspects of regeneration in insects
This report highlights the major breakthrough research on wound healing and tissue regeneration. Exploring and reviewing the molecular basis of regeneration can be helpful in the area of regenerative medicine advancements. The understanding gathered from this framework can potentially contribute to hypothesis designing with implications in the field of synthetic biology and human health.PMID:38160963 | DOI:10.1016/j.ydbio.2023.12.011 (Source: Developmental Biology)
Source: Developmental Biology - December 31, 2023 Category: Biology Authors: Shivali Pandita Sanchita Singh Sanjay Kumar Bajpai Geetanjali Mishra Gauri Saxena Praveen C Verma Source Type: research
Molecular aspects of regeneration in insects
This report highlights the major breakthrough research on wound healing and tissue regeneration. Exploring and reviewing the molecular basis of regeneration can be helpful in the area of regenerative medicine advancements. The understanding gathered from this framework can potentially contribute to hypothesis designing with implications in the field of synthetic biology and human health.PMID:38160963 | DOI:10.1016/j.ydbio.2023.12.011 (Source: Developmental Biology)
Source: Developmental Biology - December 31, 2023 Category: Biology Authors: Shivali Pandita Sanchita Singh Sanjay Kumar Bajpai Geetanjali Mishra Gauri Saxena Praveen C Verma Source Type: research
Molecular aspects of regeneration in insects
This report highlights the major breakthrough research on wound healing and tissue regeneration. Exploring and reviewing the molecular basis of regeneration can be helpful in the area of regenerative medicine advancements. The understanding gathered from this framework can potentially contribute to hypothesis designing with implications in the field of synthetic biology and human health.PMID:38160963 | DOI:10.1016/j.ydbio.2023.12.011 (Source: Developmental Biology)
Source: Developmental Biology - December 31, 2023 Category: Biology Authors: Shivali Pandita Sanchita Singh Sanjay Kumar Bajpai Geetanjali Mishra Gauri Saxena Praveen C Verma Source Type: research
Molecular aspects of regeneration in insects
This report highlights the major breakthrough research on wound healing and tissue regeneration. Exploring and reviewing the molecular basis of regeneration can be helpful in the area of regenerative medicine advancements. The understanding gathered from this framework can potentially contribute to hypothesis designing with implications in the field of synthetic biology and human health.PMID:38160963 | DOI:10.1016/j.ydbio.2023.12.011 (Source: Developmental Biology)
Source: Developmental Biology - December 31, 2023 Category: Biology Authors: Shivali Pandita Sanchita Singh Sanjay Kumar Bajpai Geetanjali Mishra Gauri Saxena Praveen C Verma Source Type: research
Molecular aspects of regeneration in insects
This report highlights the major breakthrough research on wound healing and tissue regeneration. Exploring and reviewing the molecular basis of regeneration can be helpful in the area of regenerative medicine advancements. The understanding gathered from this framework can potentially contribute to hypothesis designing with implications in the field of synthetic biology and human health.PMID:38160963 | DOI:10.1016/j.ydbio.2023.12.011 (Source: Developmental Biology)
Source: Developmental Biology - December 31, 2023 Category: Biology Authors: Shivali Pandita Sanchita Singh Sanjay Kumar Bajpai Geetanjali Mishra Gauri Saxena Praveen C Verma Source Type: research
Anp32e protects against accumulation of H2A.Z at Sox motif containing promoters during zebrafish gastrulation
Dev Biol. 2023 Dec 28:S0012-1606(23)00212-9. doi: 10.1016/j.ydbio.2023.12.010. Online ahead of print.ABSTRACTEpigenetic regulation of chromatin states is crucial for proper gene expression programs and progression during development, but precise mechanisms by which epigenetic factors influence differentiation remain poorly understood. Here we find that the histone variant H2A.Z accumulates at Sox motif-containing promoters during zebrafish gastrulation while neighboring genes become transcriptionally active. These changes coincide with reduced expression of anp32e, the H2A.Z histone removal chaperone, suggesting that loss ...
Source: Developmental Biology - December 30, 2023 Category: Biology Authors: Fabian N Halblander Fanju W Meng Patrick J Murphy Source Type: research
