CXCL6: A potential therapeutic target for inflammation and cancer
AbstractChemokines were originally defined as cytokines that affect the movement of immune cells. In recent years, due to the increasing importance of immune cells in the tumor microenvironment (TME), the role of chemokines has changed from a single"chemotactic agent" to a key factor that can regulate TME and affect the tumor phenotype. CXCL6, also known as granulocyte chemoattractant protein-2 (GCP-2), can recruit neutrophils to complete non-specific immunity in the process of inflammation. Cancer-related genes and interleukin family can promote the abnormal secretion of CXCL6, which promotes tumor growth, metas...
Source: Clinical and Experimental Medicine - August 23, 2023 Category: Research Source Type: research
Immune landscape and oncobiota in HPV-Associated Colorectal Cancer: an explorative study
AbstractWorldwide more than 550,000 new patients suffering from malignant tumors are associated with human papillomaviruses (HPV) infection. However, only a small portion of patients infected progress to cancer, suggesting that other factors other than HPV may play a role. Some studies have investigated HPV infection in colorectal cancer (CRC) with discordant results; moreover, the role of HPV in CRC development is still unknown. We investigated HPV infection in 50 CRC from different regions, excluding the anal one, by immunohistochemistry (IHC), real-time PCR and RNA-seq. For each patient, we studied the tumor microenviro...
Source: Clinical and Experimental Medicine - August 23, 2023 Category: Research Source Type: research
Correction to: The high expression of FOXE1 in colorectal cancer predicts a promising prognosis: a retrospective study
(Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - August 19, 2023 Category: Research Source Type: research
Impact of methotrexate on humoral and cellular immune responses to SARS-CoV-2 mRNA vaccine in patients with rheumatoid arthritis
AbstractThe aim of this study was to longitudinally evaluate the undetermined impact of methotrexate (MTX) on the cumulative immunogenicity elicited by three doses of SARS-CoV-2 mRNA vaccination in patients with rheumatoid arthritis (RA). We prospectively evaluated vaccine-induced immune responses following the first dose, 1 and 6 months after the second dose, and 1 month after the third dose of BNT162b2 or mRNA-1273 in 144 SARS-CoV-2 naïve participants (70 patients with RA, 29 disease controls without immunosuppressive conditions, and 45 healthy controls). Humoral immune responses were assessed by quantifying anti-s...
Source: Clinical and Experimental Medicine - August 16, 2023 Category: Research Source Type: research
The performance of the 2022 ACR/EULAR classification criteria for Takayasu ’s arteritis as compared to the 1990 ACR classification criteria in a Chinese population
In this study, we studied the performance of the 2022 American College of Rheumatology (ACR)/ European Alliance of Associations for Rheumatology (EULAR) classification criteria for Takayasu's arteritis (TAK) as compared to the 1990 ACR classification criteria in a Chinese population. The sensitivity, specificity, positive predictive value, negative predictive value, accuracy and the area under the receiver operating characteristics curve (AUC) of the above two criteria were compared. The sensitivity (92.6%), positive predictive value (95.6%), negative predictive value (94.6%), accuracy (95.0%) and AUC (0.981) of the 2022 c...
Source: Clinical and Experimental Medicine - August 15, 2023 Category: Research Source Type: research
Alterations of the intestinal microbiome and metabolome in women with rheumatoid arthritis
AbstractRheumatoid arthritis (RA) is more common in women, and many reports of sex differences have been reported in various aspects of RA. However, there has been a lack of specific research on women ’s gut flora. To assess the association between the gut flora and RA patients, this study combined the microbiome with metabolomics. Fecal samples from RA patients and healthy controls were collected for 16S rRNA sequencing. Nontargeted liquid chromatography–mass spectrometry was used to detect metabolites in fecal samples. We comprehensively used various analytical methods to reveal changes in intestinal flora and metabo...
Source: Clinical and Experimental Medicine - August 12, 2023 Category: Research Source Type: research
Somatic ARAF mutations in pediatric Langerhans cell histiocytosis: clinicopathologic, genetic and functional profiling
AbstractARAF mutations have been identified in a limited subset of patients with Langerhans cell histiocytosis (LCH), a rare disorder characterized by abnormal proliferation of Langerhans cells. LCH is primarily instigated by mutations in the mitogen-activated protein kinase (MAPK) signaling pathway, withBRAFV600E andMAP2K1 mutations constituting most cases.ARAF mutations in LCH highlight the heterogeneity of the disease and provide insights into its underlying molecular mechanisms. However, the occurrence ofARAF-positive LCH cases is extremely rare, with only two reported globally. Although they may be linked to a more ag...
Source: Clinical and Experimental Medicine - August 12, 2023 Category: Research Source Type: research
Selective detection of HBV pre-genomic RNA in chronic hepatitis B patients using a novel RT-PCR assay
AbstractIn chronic hepatitis B (CHB) patients, quantification of HBV pgRNA in plasma has the potential to provide information on disease prognosis and liver injury or histopathology. However, current methods for detecting HBV pgRNA present technical difficulties due to the co-existence of HBV DNA in plasma samples. We have successfully established a novel one-step RT-PCR assay that allows selective quantification of HBV pgRNA. Two cohorts of participants were recruited for assay validation, including treatment-na ïve patients with CHB and HBeAg-positive CHB patients who were treated with Tenofovir and monitored for 6 mon...
Source: Clinical and Experimental Medicine - August 12, 2023 Category: Research Source Type: research
Alterations of the intestinal microbiome and metabolome in women with rheumatoid arthritis
AbstractRheumatoid arthritis (RA) is more common in women, and many reports of sex differences have been reported in various aspects of RA. However, there has been a lack of specific research on women ’s gut flora. To assess the association between the gut flora and RA patients, this study combined the microbiome with metabolomics. Fecal samples from RA patients and healthy controls were collected for 16S rRNA sequencing. Nontargeted liquid chromatography–mass spectrometry was used to detect metabolites in fecal samples. We comprehensively used various analytical methods to reveal changes in intestinal flora and metabo...
Source: Clinical and Experimental Medicine - August 12, 2023 Category: Research Source Type: research
Somatic ARAF mutations in pediatric Langerhans cell histiocytosis: clinicopathologic, genetic and functional profiling
AbstractARAF mutations have been identified in a limited subset of patients with Langerhans cell histiocytosis (LCH), a rare disorder characterized by abnormal proliferation of Langerhans cells. LCH is primarily instigated by mutations in the mitogen-activated protein kinase (MAPK) signaling pathway, withBRAFV600E andMAP2K1 mutations constituting most cases.ARAF mutations in LCH highlight the heterogeneity of the disease and provide insights into its underlying molecular mechanisms. However, the occurrence ofARAF-positive LCH cases is extremely rare, with only two reported globally. Although they may be linked to a more ag...
Source: Clinical and Experimental Medicine - August 12, 2023 Category: Research Source Type: research
Selective detection of HBV pre-genomic RNA in chronic hepatitis B patients using a novel RT-PCR assay
AbstractIn chronic hepatitis B (CHB) patients, quantification of HBV pgRNA in plasma has the potential to provide information on disease prognosis and liver injury or histopathology. However, current methods for detecting HBV pgRNA present technical difficulties due to the co-existence of HBV DNA in plasma samples. We have successfully established a novel one-step RT-PCR assay that allows selective quantification of HBV pgRNA. Two cohorts of participants were recruited for assay validation, including treatment-na ïve patients with CHB and HBeAg-positive CHB patients who were treated with Tenofovir and monitored for 6 mon...
Source: Clinical and Experimental Medicine - August 12, 2023 Category: Research Source Type: research
Causal inference between rheumatoid arthritis and prostate cancer
AbstractIt is unclear if the association between rheumatoid arthritis (RA) and a higher risk of prostate cancer (Pca) reflects a causal relationship. We conducted a meta-analysis and used the Mendelian randomization method (MR) to evaluate the association between RA and Pca risk. A meta-analysis and subgroup analysis of the incidence of Pca in patients with RA was conducted. To determine whether genetically elevated RA levels were causally linked to Pca, two MR samples were employed. To eliminate gender-related bias, we conducted a stratified analysis of the GWAS data for RA by gender, specifically including 140,254 males....
Source: Clinical and Experimental Medicine - August 11, 2023 Category: Research Source Type: research
