Alterations of the intestinal microbiome and metabolome in women with rheumatoid arthritis

AbstractRheumatoid arthritis (RA) is more common in women, and many reports of sex differences have been reported in various aspects of RA. However, there has been a lack of specific research on women ’s gut flora. To assess the association between the gut flora and RA patients, this study combined the microbiome with metabolomics. Fecal samples from RA patients and healthy controls were collected for 16S rRNA sequencing. Nontargeted liquid chromatography–mass spectrometry was used to detect metabolites in fecal samples. We comprehensively used various analytical methods to reveal changes in intestinal flora and metabolites in female patients. The gut flora of RA patients was significantly different from that of healthy women. The abundance ofBacteroides,Megamonas andOscillospira was higher in RA patients, while the abundance ofPrevotella,Gemmiger andRoseburia was lower than that of healthy women.Gemmiger,Bilophila andOdoribacter represented large differences in microflora between RA and healthy women and could be used as potential microorganisms in the diagnosis. Fatty acid biosynthesis was significantly different between RA patients and healthy women in terms of metabolic pathways. There were different degrees of correlation between the gut flora and metabolites. Lys-Phe-Lys and heptadecasphin-4-enine can be used as potential markers for RA diagnosis. There was an extremely significant positive correlation betweenMegamonas,Dialister and rheumatoid factors, which was fou...
Source: Clinical and Experimental Medicine - Category: Research Source Type: research