Role Of TP53 mutations in predicting the clinical efficacy of hypomethylating therapy in patients with myelodysplastic syndrome and related neoplasms: a systematic review and meta-analysis
Abstract Hypomethylating agents (HMAs) are now a major treatment option for myelodysplastic syndrome (MDS) and related neoplasms, but 50% of patients still do not respond and realize poor outcomes. Mutational predictors of treatment efficacy attract continuous attention. Whether TP53 mutations can be used as predictors of HMA effectiveness has caused heated debate. Therefore, we performed a meta-analysis to investigate the predictive value of TP53 mutations to outcomes of HMA therapy in patients with MDS and related neoplasms. We systematically searched PubMed, Embase, the Cochrane Library, and the WanFang databases (publi...
Source: Clinical and Experimental Medicine - July 1, 2020 Category: Research Source Type: research

IGFBP3 as an indicator of lymph node metastasis and unfavorable prognosis for papillary thyroid carcinoma
In conclusion, IGHBP3 might be involved in the development of LNM in PTC patients. IGHBP3 over-expression might be a novel indicator and a potential target for PTC therapy. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - June 28, 2020 Category: Research Source Type: research

Six long noncoding RNAs as potentially biomarkers involved in competitive endogenous RNA of hepatocellular carcinoma
AbstractTo investigate lncRNAs acting as competing endogenous RNAs (ceRNAs) involved in oncogenesis and progression of HCC. Different expressed lncRNAs, microRNAs, and mRNAs (DElncRNAs, DEmiRNAs, DEmRNAs), downloaded from The Cancer Genome Atlas (TCGA) database, were identified by edgeR package. CeRNA network was constructed based on miRcode, TargetScan, and miRTarBase. Target DEmRNAs were annotated by KEGG pathway and GO analysis. Negatively correlated lncRNA-miRNA pairs were analyzed by Pearson correlation coefficient, simultaneously, overall survival (OS) were evaluated. The expression of these lncRNAs were examined in ...
Source: Clinical and Experimental Medicine - June 9, 2020 Category: Research Source Type: research

Expression of BAFF and BAFF receptors in primary Sj ögren’s syndrome patients with ectopic germinal center-like structures
AbstractB cell-activating factor (BAFF) is an essential cytokine in primary Sj ögren’s syndrome (pSS) physiopathology. It has been reported that pSS patients develop germinal center-like (GC-like) structures in their minor salivary glands (MSGs). BAFF, BAFF-R, TACI, and BCMA expression was analyzed in MSGs from 29 subjects (nonspecific chronic sialadenitis and focal lymphoc ytic sialadenitis with the presence [pSS-GC(+)] or absence [pSS-GC(−)] of GC-like structures). Twenty-four percent of patients showed ectopic GC-like structures and a high focus score [p 
Source: Clinical and Experimental Medicine - June 6, 2020 Category: Research Source Type: research

The diagnostic and prognostic value of exosome-derived long non-coding RNAs in cancer patients: a meta-analysis
AbstractPurposeExosome-derived long non-coding RNAs (lncRNAs) as novel biomarkers are widely investigated in various cancers, yet results remain controversial. The aim of this meta-analysis was to clarify the diagnostic and prognostic value of exosome-derived lncRNAs in cancer.MethodsPubMed, Web of Science, EMBASE, CNKI, and WanFang online databases were comprehensively searched for eligible studies up to January, 2020. To evaluate the diagnostic effect, sensitivity, specificity, and area under the curve (AUC) were pooled. Threshold effect, subgroup analysis, and meta-regression were applied to explore heterogeneity. Deeks...
Source: Clinical and Experimental Medicine - June 5, 2020 Category: Research Source Type: research

LncPRYP4-3 serves as a novel diagnostic biomarker for dissecting subtypes of metabolic associated fatty liver disease by targeting RPS4Y2
AbstractLongitudinal studies have improved current diagnostics and management of metabolic associated fatty liver disease (MAFLD) patients by liver biopsy and therapeutic intervention, yet the deficiency of biomarker spectrum for dissecting subtypes largely hinders the symptomatic treatment. We originally enriched serum from peripheral blood of 618 healthy donors (HD) and 580 MAFLD (400 NAFL, 180 NASH) patients according to multiple clinicopathological indicators. Microarray profiling and qRT-PCR were conducted to identify lncRNAs as candidate biomarkers of MAFLD. Then, we analyzed the matching score of the indicated lncRN...
Source: Clinical and Experimental Medicine - June 3, 2020 Category: Research Source Type: research

Retrospective analysis of different regimens for Chinese adults with severe newly diagnosed immune thrombocytopenia
AbstractTo review the efficacy and safety of glucocorticoids combined with different regimens for treating severe immune thrombocytopenia (ITP). Eighty-five severe ITP patients from 2 tertiary hospitals treated with glucocorticoids were enrolled from January 2018 to May 2019 and divided into 4 treatment groups: group A (treated with glucocorticoids), group B (glucocorticoids plus intravenous immunoglobulin (IVIg)), group C (glucocorticoids plus recombinant human thrombopoietin (rhTPO)), and group D (glucocorticoids plus IVIg and rhTPO). Statistical analysis was performed with SPSS 19.0 software. Early responses and respons...
Source: Clinical and Experimental Medicine - May 26, 2020 Category: Research Source Type: research

The effects of apoptosis and apelin on lymph node metastasis in invasive breast carcinomas
This study aimed to evaluate the biological and clinical significance of apelin-36 in breast cancer and to compare apelin-36 expression and apoptotic index in both breast tissue and metastatic lymph nodes in patients with invasive breast carcinoma. In this study, both tumor tissue and metastatic lymph nodes of the same patient were collected from 60 cases of invasive breast carcinoma patients (IDC, ILC) and 20 cases of normal breast tissue with no tumor from mammoplasty were used as the control group. The expression of apelin was examined with immunohistochemically, and the apoptotic index was examined with TUNEL methods. ...
Source: Clinical and Experimental Medicine - May 24, 2020 Category: Research Source Type: research

The current state of MiRNAs as biomarkers and therapeutic tools
AbstractMicroRNAs (miRNAs) are non-coding RNAs with a length of 18 –22 nucleotides that regulate about a third of the human genome at the post-transcriptional level. MiRNAs are involved in almost all biological processes, including cell proliferation, apoptosis, and cell differentiation, but also play a key role in the pathogenesis of many diseases. Most miRNAs a re expressed within the cells themselves. Due to various forms of transport from cells like exosomes, circulating miRNAs are stable and can be found in human body fluids, such as blood, saliva, cerebrospinal fluid, and urine. Circulating miRNAs are of great ...
Source: Clinical and Experimental Medicine - May 12, 2020 Category: Research Source Type: research

Long-term efficacy, safety, and tolerability of recombinant human hyaluronidase-facilitated subcutaneous infusion of immunoglobulin (Ig) (fSCIG; HyQvia( ® )) in immunodeficiency diseases: real-life data from a monocentric experience
AbstractHumoral immunodeficiency diseases represent a heterogeneous group of disorders that require long-term therapies. Thus, the treatment provided must not only be effective but also safe and well tolerated. In this paper, we report our data on the efficacy, safety, and tolerability of recombinant human hyaluronidase-facilitated subcutaneous infusion of immunoglobulin (Ig) (fSCIG; HyQvia(®)) in immunodeficiency patients. We collected retrospective data from 30 patients with primary and secondary immunodeficiency diseases in therapy with fSCIG from September 2014 to December 2019. We evaluated the efficacy of the the...
Source: Clinical and Experimental Medicine - May 8, 2020 Category: Research Source Type: research

Association between HER2 and IL-6 genes polymorphisms and clinicopathological characteristics of breast cancer: significant role of genetic variability in specific breast cancer subtype
This study aimed to evaluate the association between HER2 (rs2517956) and (IL-6) (rs1800795 and rs2069837) and clinicopathological characteristics in HER2-positive and HER2-negative breast cancer in Tunisian women. A retrospective cohort study included 273 patients. Genomic DNA was extracted from peripheral blood samples, and genotyping of selected SNP was performed by PCR –RFLP assays. Statistical analysis was then carried out to assess genotypic frequencies and genetic association in relation to breast cancer subtypes. SHEsis software was applied to IL-6 haplotypic structure analysis. The distribution of genotype f...
Source: Clinical and Experimental Medicine - May 5, 2020 Category: Research Source Type: research

CYP4F2 and CYP3A5 gene polymorphisms and lung cancer in Chinese Han population
This study aimed to explore whether the polymorphisms ofCYP4F2 andCYP3A5 are correlated with the risk of lung cancer development. A case –control study was conducted among 510 patients with pathologically confirmed lung cancer as the case group and 504 healthy individuals as the control group. Four single-nucleotide polymorphisms of theCYP4F2 andCYP3A5 genes were genotyped, and their correlations with the risk of lung cancer were examined using Chi-square test and logistic regression analysis. Stratified analysis found that the rs3093105 and rs3093106 loci ofCYP4F2 gene were significantly associated with lower risk o...
Source: Clinical and Experimental Medicine - April 30, 2020 Category: Research Source Type: research

Clinical practice: chimeric antigen receptor (CAR) T cells: a major breakthrough in the battle against cancer
AbstractChimeric antigen receptor (CAR) T cell therapy has come of age, offering a potentially curative option for patients who are refractory to standard anti-cancer treatments. The success of CAR T cell therapy in the setting of acute lymphoblastic leukemia and specific types of B cell lymphoma led to rapid regulatory approvals of CD19-directed CAR T cells, first in the United States and subsequently across the globe. Despite these major milestones in the field of immuno-oncology, growing experience with CAR T cells has also highlighted the major limitations of this strategy, namely challenges associated with manufacturi...
Source: Clinical and Experimental Medicine - April 24, 2020 Category: Research Source Type: research

The association of neutrophil-to-lymphocyte ratio with all-cause mortality in Chinese patients with MPO-ANCA associated vasculitis
This study was thus undertaken to explore the relationship between NLR at diagnosis with inflammatory response and disease activity among MPO-AAV patients in a single Chinese center. Furthermore, we evaluated whether NLR could predict the renal prognosis and patient outcome. 188 patients with MPO-AAV were included in this study. Baseline NLR was positively correlated with CRP (r = 0.404,P 
Source: Clinical and Experimental Medicine - April 21, 2020 Category: Research Source Type: research

IL-10 suppresses IFN- γ-mediated signaling in lung adenocarcinoma
In this study, we examined the expression of IL-10 and IL-10 receptor 1 (IL-10R1) in resected locally advanced lung adenocarcinoma by immunohistochemistry. IL-10 immunoreactivity was stronger in intraepithelial regions than in stroma. The amount of IL-10 found either in intraepithelial or in stromal regions had no prognostic value, but the relative distribution of IL-10 in these two locations was related to cancer-immune phenotypes. High expression of IL-10R1 by tumor cells was significantly correlated with poor prognosis, suggesting that IL-10-mediated signaling may induce cancer cell intrinsic effects that promote cancer...
Source: Clinical and Experimental Medicine - April 18, 2020 Category: Research Source Type: research

MicroRNA-4317 predicts the prognosis of breast cancer and inhibits tumor cell proliferation, migration, and invasion
AbstractPrevious researches have indicated that miR-4317 was aberrantly expressed in several tumors. However, the potential role of miR-4317 in breast cancer is still unclear. The aim of this study was to investigate the potential role of miR-4317 in breast cancer. The relative expression levels of miR-4317 were detected in breast cancer tissues and cell lines using qRT-PCR analysis. The Kaplan –Meier survival curve and multivariate Cox regression analyses were used to investigate the prognostic significance of miR-4317 in breast cancer. CCK-8 and Transwell assays were performed to evaluate the effects of miR-4317 on...
Source: Clinical and Experimental Medicine - April 11, 2020 Category: Research Source Type: research

Top 100 most-cited articles on exosomes in the field of cancer: a bibliometric analysis and evidence mapping
This study aims to identify the general characteristics, cooperation of authors, countries and explore the hot topics of the top 100 cited articles on tumor-related exosomes. We searched the Web of Science Core Collection on June 28, 2019, to collect related publications. Microsoft Excel 2016 and VOSviewer 1.6.9 were applied to analyze the publication year, citations, authors, countries, institutions, journals, and keywords. The top 100 articles were published between 1998 and 2017 with citations ranging from 181 to 2275.Cancer Research (n = 9) had the highest number of publications.Nature Cell Biology,Nature...
Source: Clinical and Experimental Medicine - April 7, 2020 Category: Research Source Type: research

Angiopoietin-like protein 2 as a novel marker for patients with primary Sjogren ’s syndrome-related interstitial lung disease
AbstractAngiopoietin-like protein 2 (Angptl2) plays a key role in chronic inflammation and tissue remodeling. We evaluated whether serum Angptl2 is associated with interstitial lung disease (ILD) in primary Sjogren ’s syndrome (pSS) patients. A total of 158 consecutive pSS patients and 25 normal healthy controls, which completed lung HRCT, were enrolled in our research. The levels of serum Angptl2 and TGF-β1 were measured by enzyme-linked immunosorbent assay. We investigated the correlation between the acti vity indexes of pSS-ILD patients and the serum Angptl2 levels. There were 71 of 158 (44.94%) patients inte...
Source: Clinical and Experimental Medicine - April 6, 2020 Category: Research Source Type: research

Prospects and challenges of circulating tumor DNA in precision medicine of hepatocellular carcinoma
AbstractThe growing role of precision medicine in hepatocellular carcinoma (HCC) is expected to ameliorate the poor prognosis and high mortality of this highly malignant disease; however, it is faced with challenges such as the low frequency of tissue biopsy. Hence, attention is turning to the circulating tumor DNA (ctDNA), an important component of liquid biopsy. Obtaining molecular information about cancer from blood provides a good prospect in precision oncology including molecular diagnosis, molecular classification, targeted therapy, personalized decision making, and detection of drug-resistance mutations. However, in...
Source: Clinical and Experimental Medicine - April 1, 2020 Category: Research Source Type: research

Cross-sectional associations between angiotensin-converting enzyme inhibitor use and cancer diagnosis in US adults
In conclusion, in this large representative sample of US adults, it was found that ACE inhibit or use may have a marginal influence on some cancers. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - March 26, 2020 Category: Research Source Type: research

Altered ratio of circulating follicular regulatory T cells and follicular helper T cells during primary EBV infection
AbstractFollicular help T cells (Tfh) play an important role in the activation and differentiation of B cells, while follicular regulatory T cells (Tfr) control Tfh and resulting humoral immune responses. Accumulating evidence has demonstrated that the dysregulation of Tfr contributed to the pathogenesis of infectious diseases. However, the role of Tfr in Epstein –Barr virus (EBV) infection remains lacking. Fifty-five EBV-infected infectious mononucleosis (IM) patients and 21 healthy individuals (HIs) were recruited in the study. We investigated the number of Tfr (FoxP3+CXCR5+PD-1+CD4+) and Tfh (FoxP3−CXCR5+PD-...
Source: Clinical and Experimental Medicine - March 23, 2020 Category: Research Source Type: research

MUC4 isoforms expression profiling and prognosis value in Chinese melanoma patients
In conclusion, our results indicate that the dy namic MUC4 isoforms expressed in melanoma, and MUC4 isoform d and f might be served as a novel prognostic indicator of melanoma patients. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - March 14, 2020 Category: Research Source Type: research

“Mixed connective tissue disease”: a condition in search of an identity
AbstractMixed connective tissue disease was first described as a new autoimmune rheumatic disease in 1972 based on the claim of a distinct clinical picture associated with anti-RNP antibody positivity. Subsequently, this new entity has divided opinions in the rheumatology community. We have reviewed recent cohort studies with more than 100 patients, comparing the clinical and immunological features, treatment, prognosis and evolution to well-defined autoimmune rheumatic diseases. We also reviewed clinical features of undifferentiated autoimmune rheumatic diseases based on the most recent studies. After gathering and review...
Source: Clinical and Experimental Medicine - March 4, 2020 Category: Research Source Type: research

Direct-acting antiviral treatment downregulates immune checkpoint inhibitor expression in patients with chronic hepatitis C
AbstractChronic hepatitis C (CHC) infection is associated with increased TIM-3, PD-1 immune checkpoint receptors expression that inhibits adaptive T cells and increases NK cell cytotoxicity against T helper cells, both resulting T cell exhaustion. Elimination of the virus with direct-acting antivirals (DAAs) may modify host immune response via altering these immune checkpoint receptors ’ expression. We conducted a prospective study to analyze changes in TIM-3, PD-1 and their ligands galectin-9, PD-L1 expression by peripheral blood T cell subpopulations, NK cell subpopulations, and monocytes by multicolor flow cytomet...
Source: Clinical and Experimental Medicine - February 27, 2020 Category: Research Source Type: research

Patient-derived xenograft models for personalized medicine in colorectal cancer
AbstractEstablishing superior preclinical models is critical for translational cancer research owing to the high failure rates of novel therapeutics in clinical studies. Even though cell line-derived xenograft models are easy to create, they have numerous limitations since these models do not represent the distinctive features of each cancer patient adequately. To circumvent the discrepancies between xenograft models and tumors, patient-derived xenograft (PDX) models have been developed. These models are established through the engraftment of tissue from a patient ’s tumor into an immune-deficient mouse, which preser...
Source: Clinical and Experimental Medicine - February 25, 2020 Category: Research Source Type: research

Pentraxin-3 is not related to disease severity in cirrhosis and hepatocellular carcinoma patients
AbstractThe acute-phase protein pentraxin-3 (PTX3) is a component of the innate immune system. Inflammation and tissue injury increased PTX3 in the injured liver, and accordingly, circulating PTX3 was induced in patients with chronic liver diseases. In the present study, PTX3 protein was determined in systemic, hepatic, and portal vein plasma of patients with liver cirrhosis to assess a possible association between hepatic PTX3 release and extent of liver injury. However, PTX3 levels were not related to disease severity. Of note, portal PTX3 levels were higher than concentrations in the hepatic vein. PTX3 in the hepatic an...
Source: Clinical and Experimental Medicine - February 20, 2020 Category: Research Source Type: research

Risk factors for treatment resistance and relapse of Chinese patients with MPO-ANCA-associated vasculitis
AbstractIdentification of risk factors for treatment resistance and relapse would be crucial to personalization therapy in patients with myeloperoxidase (MPO)-anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (MPO-AAV). Current evidence with regard to the risk factors for treatment resistance and relapse remains limited and inconclusive. We aimed to assess the predictors for treatment resistance and relapse in a single-center cohort of Chinese patients with MPO-AAV in this study. In total, 184 patients with MPO-AAV were included. Treatment resistance occurred in 64 (34.9%) of 184 patients and was positively...
Source: Clinical and Experimental Medicine - February 20, 2020 Category: Research Source Type: research

Viral clearance ameliorates hematological and inflammatory markers among diabetic patients infected with hepatitis C genotype 4
AbstractThe achievement of virological response in the treatment of hepatitis C virus (HCV) can improve the extrahepatic manifestations. The present study aimed to investigate the effect of HCV eradication after sofosbuvir/daclatasvir (SOF/DCV) therapy on hematological and inflammatory biomarkers in type 2 diabetic patients infected with HCV genotype 4. Between October 2017 and August 2018, among 145 patients with HCV genotype 4, 30 patients were enrolled in the study based on the fact that they have type 2 diabetes. Enrolled HCV-diabetic patients were treated for 12  weeks with SOF/DCV regimen. Patients were screened...
Source: Clinical and Experimental Medicine - February 19, 2020 Category: Research Source Type: research

Extramedullary blastic transformation of primary myelofibrosis in the form of disseminated myeloid sarcoma: a case report and review of the literature
AbstractSplenomegaly is a key clinical manifestation of myelofibrosis, and splenectomy is currently indicated in patients with drug refractory, symptomatic splenomegaly or with the aim of improving refractory cytopenias. Transformation to acute myeloid leukemia occurs in up to 20% of patients with myelofibrosis, while cases of myeloid sarcoma have been reported very unfrequently. In this manuscript, we report the case of a 60-year-old man with a history of primary myelofibrosis who underwent splenectomy because of drug-refractory massive splenomegaly, systemic symptoms and anemia. At the opening of the peritoneal cavity, t...
Source: Clinical and Experimental Medicine - February 17, 2020 Category: Research Source Type: research

Hepatitis B core antibody and liver stiffness measurements predict HBeAg seroconversion in HBeAg-positive chronic hepatitis B patients with minimally elevated alanine aminotransferase (ALT) levels
In this study, a total of 106 patients with CHB were enrolled and treated with entecavir, telbivudine or teno fovir disoproxil fumarate. Liver stiffness was measured by transient elastography, and quantitative levels of hepatitis B core antibody (HBcAb) were detected by ELISA. At week 96, 31 (29.25%) patients achieved hepatitis B e antigen (HBeAg) seroconversion. Notably, baseline HBcAb levels and liver sti ffness measurements (LSM) were higher in patients who achieved HBeAg seroconversion. The multivariate analysis showed that the baseline HBcAb levels and LSM were independent predictors for HBeAg seroconversion. The area...
Source: Clinical and Experimental Medicine - February 12, 2020 Category: Research Source Type: research

HCV infection and non-Hodgkin lymphomas: an evolving story
AbstractHepatitis C virus infection represents a global health problem with 3% of population infected worldwide. Several epidemiological studies have shown an increased risk of B cell non-Hodgkin lymphomas in HCV-infected subjects with a wide geographic variability. The observation that HCV eradication by antiviral treatment is associated with successful lymphoma response provided the most convincing evidence for the causal role of HCV in lymphoma ’s development. According to the most accepted model, HCV-driven chronic antigenic stimulation may represent the major stimulus for lymphoma growth. Several evidences have ...
Source: Clinical and Experimental Medicine - February 12, 2020 Category: Research Source Type: research

The prognostic role of CD133 expression in patients with osteosarcoma
This study was conducted to investigate the association between CD133 expression and OS. We performed a search using several database including NCBI PubMed, Springer, CNKI, Web of Science and Wanfang for relevant papers about the prognostic value of CD133 on OS published before October 19, 2019. Pooled odds ratio (OR) and 95% CI were used to evaluate the outcomes. Seven reports including 498 OS patients were used for evaluating the association between CD133 positive expression and OS clinicopathological features. CD133 positive expression was not related to the age, gender, tumor size, tumor location and pathological type ...
Source: Clinical and Experimental Medicine - February 12, 2020 Category: Research Source Type: research

Spectrum and prognosis of renal histopathological lesions in 56 Chinese patients with rheumatoid arthritis with renal involvement
The objective of the study was to evaluate the characteristics and prognosis of 56 patients with rheumatoid arthritis (RA)-associated renal involvement by retrospective review of their renal biopsy specimens. Included in this cross-sectional study were 56 RA patients with renal involvement, in whom renal biopsy was performed to analyze the histological pattern and renal prognosis. IgA nephropathy (IgAN) was detected in 48.2% of the 56 included patients as the most common renal histological pattern, followed by membranous nephropathy (MN) in 23.2% cases, focal segmental glomerular sclerosis (FSGS) in 19.6% cases, chronic in...
Source: Clinical and Experimental Medicine - February 11, 2020 Category: Research Source Type: research

Correction to: BAL biomarkers ’ panel for differential diagnosis of interstitial lung diseases
The original version of this article unfortunately contained a mistake. First and last names of the authors were interchanged. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - February 8, 2020 Category: Research Source Type: research

Systemic treatment of the metastatic renal cell carcinoma: usefulness of the apparent diffusion coefficient of diffusion-weighted MRI in prediction of early therapeutic response
AbstractAccurate prediction of early treatment response to systemic therapy (ST) with tyrosine kinase inhibitors (TKI) in patients with metastatic renal cell carcinoma (mRCC) could help avoid ineffective and expensive treatment with serious side effects. Neither RECIST v.1.1 nor Choi criteria successfully discriminate between patients with mRCC who received ST having a short or long time to progression (TTP). There is no biomarker, which is able to predict early therapeutic response to TKIs application in patients with mRCC. The goal of our study was to investigate the potential of apparent diffusion coefficient (ADC) of d...
Source: Clinical and Experimental Medicine - February 5, 2020 Category: Research Source Type: research

Dietary phytochemicals as the potential protectors against carcinogenesis and their role in cancer chemoprevention
AbstractHealth-threatening consequences of carcinogen exposure are mediated via occurrence of electrophiles or reactive oxygen species. As a result, the accumulation of biomolecular damage leads to the cancer initiation, promotion or progression. Accordingly, there is an association between lifestyle factors including inappropriate diet or carcinogen formation during food processing, mainstream, second or third-hand tobacco smoke and other environmental or occupational carcinogens and malignant transformation. Nevertheless, increasing evidence supports the protective effects of naturally occurring phytochemicals against ca...
Source: Clinical and Experimental Medicine - February 3, 2020 Category: Research Source Type: research

B7-H4 facilitates proliferation and metastasis of colorectal carcinoma cell through PI3K/Akt/mTOR signaling pathway
AbstractB7-H4 is over-expressed in various tumors and may affect many aspects of cancer biology. Our previous studies have reported that the over-expressed B7-H4 in serum or tumor tissue of colorectal carcinoma (CRC) patients was closely related to CRC progression. However, B7-H4 in cell biological characteristics of CRC is not well studied. Here, we investigate the effect of the B7-H4 on cell proliferation, migration and its expression regulated by PI3K/Akt/mTOR signaling pathway in CRC. Firstly, pSilencer 4.1-B7-H4-shRNA vector was constructed and stable transfection was performed on HT-29 cells. Secondly, cell prolifera...
Source: Clinical and Experimental Medicine - February 1, 2020 Category: Research Source Type: research

Prognostic significance of serum progranulin level in de novo adult acute lymphoblastic leukemia patients
This study involved 60 subjects (40 de novo adult ALL patients and 20 controls). Serum PGRN level was measured by enzyme-linked immunosorbent assay and was correlated with patient outcome. Serum PGRN level was significantly higher in patients than controls. Serum PGRN level did not correlate with age, total leukocytic count, hemoglobin, platelets, absolute blast count in peripheral blood, lactate dehydrogenase, percent of blasts in bone marrow, gender, comorbidities, the presence of central nervous system infiltration, ALL phenotype, cytogenetics and risk of the disease. High serum PGRN level was not associated with inferi...
Source: Clinical and Experimental Medicine - January 31, 2020 Category: Research Source Type: research

Gene expression profiling revealed MCM3 to be a better marker than Ki67 in prognosis of invasive ductal breast carcinoma patients
AbstractInvasive ductal carcinoma (IDC) is the most common breast cancer. Our study used gene microarray data to select differentially expressed genes between normal and IDC mammary tissues. From these, we selected genes related to the proliferation of tumor cells and compared their prognostic value with known biomarker Ki67 for IDC. Analysis of publicly available Gene Expression Omnibus (GEO) data revealed 24 differentially expressed genes (DEGs) in normal and 31 DEGS in IDC tissues that were used for further analyses. Gene chip analysis software was used to identify DEGs. DEG profiles were confirmed using quantitative PC...
Source: Clinical and Experimental Medicine - January 24, 2020 Category: Research Source Type: research

BAL biomarkers ’ panel for differential diagnosis of interstitial lung diseases
AbstractBronchoalveolar lavage (BAL) is a useful procedure for differential diagnosis of interstitial lung diseases (ILDs) and for identification of granulomatous lung diseases. We investigated a panel of biomarkers from BAL fluid of ILD patients to evaluate their utility in differentiating ILDs. Bronchoscopy with BAL was performed in 100 consecutive patients with suspected ILD (41 sarcoidosis, 11 cHP and 24 other ILDs); the 24 patients negative for ILD diagnosis were included as control group. BAL phenotypes and cell profiles (CD4+/CD8+ ratio, NK and CD103+ cell counts, chitotriosidase and KL-6 levels in BAL) were determi...
Source: Clinical and Experimental Medicine - January 22, 2020 Category: Research Source Type: research

Correction to: Cell adhesion molecules, plasminogen activator inhibitor type 1, and metabolic syndrome in patients with psoriasis
The original version of this article unfortunately contained a mistake. Camila Cataldi de Alcantara was not listed among the authors. The corrected author group should be: (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - January 8, 2020 Category: Research Source Type: research

Diagnostic value of seven biomarkers for breast cancer: an overview with evidence mapping and indirect comparisons of diagnostic test accuracy
In conclusion, miRNA had better diagnostic accuracy than the other six biomarkers. But due to the low quality of included SRs, the results need to be interpreted with caution. Further study should investigate the diagnostic accuracy of different biomarkers in direct comparisons and focus on the value of combined biomarkers. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - January 1, 2020 Category: Research Source Type: research

Genetic and epigenetic analysis of the beta-2-microglobulin gene in microsatellite instable colorectal cancer
AbstractOne of the most common mechanisms of immune evasion in MSI colorectal cancers (CRCs) is loss of HLA class I expression due to mutations inB2M gene which can become a negative predictor for checkpoint blockade therapy. The aim of this study was the determination of prevalence ofB2M somatic mutations in MSI CRC patients and relationship betweenB2M mutations and lymphocytes infiltration and other clinicopathological features as well as detection of methylation changes inB2M promoter region which can be another mechanism of immune escape. In our study, 37 MSI-H and 5 MSI-L patients were selected for screening ofB2M mut...
Source: Clinical and Experimental Medicine - December 18, 2019 Category: Research Source Type: research

Early echocardiographic detection of left ventricular diastolic dysfunction in patients with systemic lupus erythematosus asymptomatic for cardiovascular disease
In this study, 23 SLE patients asymptomatic for CVD underwent a comprehensive echocardiographic examination to detect subclinical cardiac involvement. According to their SELENA-SLEDAI score, they were divided into two groups: SELENA-SLEDAI  ≤ 12 (n = 13, 12 females) and SELENA-SLEDAI >  12 (n = 10, all females), indicative of mild-to-moderate and severe SLE, respectively. Patients in the latter group had significant increases in left ventricular (LV) mass, LV end-diastolic volume, left atrial volume and right heart parameters (pulmonary arterial pressure, tricus...
Source: Clinical and Experimental Medicine - December 17, 2019 Category: Research Source Type: research

E3 ubiquitin ligase CHIP attenuates cellular proliferation and invasion abilities in triple-negative breast cancer cells
In this study, the CHIP expression was examined in different human breast cancer cell lines and a group of breast cancer tissues. We found, for the first time, that CHIP expression was correlated with the molecular subtyping of breast cancer. CHIP was least expressed in the base-like subtype of breast cancer, which are triple-negative breast cancer (TNBC) breast cancer predominantly. Accordingly, CHIP expression was evidently decreased in the TNBC MDA-MB-231 breast cancer cell line. Enforced induction of CHIP in the MDA-MB-231 cells exerted no obvious influences on cellular growth and cell cycle. The apoptotic and prolifer...
Source: Clinical and Experimental Medicine - December 16, 2019 Category: Research Source Type: research

Early fibrosis regression by shear wave elastography after successful direct-acting anti-HCV therapy
AbstractShear wave elastography (SWE) is a noninvasive ultrasound-based marker of hepatic fibrosis not requiring a special device. Successful direct-acting anti-HCV therapy was associated with hepatic fibrosis regression assessed by transient elastography (FibroScan). Data on the utility of SWE in these patients and how early fibrosis can regress after treatment are still lacking. To assess liver fibrosis by SWE before and after direct-acting antiviral treatment of chronic hepatitis C (CHC), we enrolled 165 CHC genotype 4 Egyptian patients treated with different Sofosbuvir-based regimens. Patients ’ laboratory charac...
Source: Clinical and Experimental Medicine - December 2, 2019 Category: Research Source Type: research

Cell adhesion molecules, plasminogen activator inhibitor type 1, and metabolic syndrome in patients with psoriasis
In conclusion, CAM levels are associated with psoriasis diagnosis and MetS may influence E-selectin and PAI-1 concentrations. More studies are needed to verify the causality among these factors, as well as their relation to the different degrees of disease severity. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - November 22, 2019 Category: Research Source Type: research

The role of fibroblast activation protein in progression and development of osteosarcoma cells
AbstractTo investigate the expression levels of fibroblast activation protein (FAP) in human osteosarcoma tissues and its possible correlations with clinical pathological characteristics of patients with osteosarcoma, and to  explore the potential effects of FAP on progression and development of osteosarcoma. Immunohistochemistry (IHC) assay was initially performed to detect the expression levels of FAP in 66 tumor tissues and adjacent non-tumor tissues. Patients were sequentially divided into two groups based on diffe rent expression levels of FAP. The correlations between the expression levels of FAP and the clinica...
Source: Clinical and Experimental Medicine - November 19, 2019 Category: Research Source Type: research

Hepatic dysfunction secondary to Kawasaki disease: characteristics, etiology and predictive role in coronary artery abnormalities
AbstractCoronary artery abnormalities (CAAs) are prominent during the acute Kawasaki disease (KD) episode and represent the major contributors to the long-term prognosis. Several meta-analysis and published scoring systems have identified hepatic dysfunction as an independent predictor of CAA risks. The medical records of 210 KD children were reviewed. Blood samples were collected from all subjects at 24  h pre-therapy and 48 h post-therapy, respectively. Liver function test (LFT) and inflammatory mediators were detected. Multivariate logistic regression analysis was conducted to identify the reliable biomarkers ...
Source: Clinical and Experimental Medicine - November 16, 2019 Category: Research Source Type: research

Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes
AbstractRapid urbanization and unhealthy dietary patterns critically increase the risk of type 2 diabetes  (T2D) in middle-aged Indians. However, despite recent evidence of senescence-associated microRNAs (SA-miRNAs) in regulating complex pathways of ageing, their expressions in middle-aged Indians with T2D remain unexplored. Hence we aimed to investigate the changes in expressions of SA-miRNAs miR-34a and miR-126 in middle-aged T2D patients. A total of 30 T2D patients and 30 controls were recruited of age 31–50 years. The expressions of plasma miR-34a and miR-126 were determined by quantitative PCR. Oxidiz...
Source: Clinical and Experimental Medicine - November 15, 2019 Category: Research Source Type: research