Advances in liquid biopsy using circulating tumor cells and circulating cell-free tumor DNA for detection and monitoring of breast cancer
This article reviews the main techniques and applications of CTCs and cfDNA in breast cancer. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - June 12, 2019 Category: Research Source Type: research
Functional polymorphisms of the receptor for the advanced glycation end product promoter gene in inflammatory bowel disease: a case –control study
AbstractThe receptor for the advanced glycation end products (RAGE) is a multiligand transmembrane receptor involved in chronic inflammation whose specific polymorphisms of the promoter gene were found to increase its transcriptional activity. We investigated the association of both allelic and genotypic -374T/A and -429T/C polymorphisms with inflammatory bowel disease. The STREGA guidelines were applied for planning and reporting. We enrolled 133 patients with Crohn ’s disease (CD), 149 with ulcerative colitis (UC), and 128 blood donors. Genomic DNA was extracted from peripheral blood leukocytes collected from each ...
Source: Clinical and Experimental Medicine - June 7, 2019 Category: Research Source Type: research
Neutrocyte-to-lymphocyte ratio predicts the presence of a replicative hepatitis C virus strand after therapy with direct-acting antivirals
AbstractResidual HCV-RNA can persist in liver tissue and peripheral blood mononuclear cells (PBMCs) long after antiviral therapy of chronic hepatitis C in patients repeatedly negative for viral RNA in serum. This occult infection associates with impaired immune response and the risk of lymphoproliferative disorders or progressive liver disease. There are currently no monitoring strategies for patients after treatment. We investigated if serum inflammation markers and interferon lambda (IFNL) genotype can be predictors of the presence of HCV-RNA and the replicative HCV-RNA ( −) strand in patients who reached sustained...
Source: Clinical and Experimental Medicine - May 24, 2019 Category: Research Source Type: research
Changes in APRI and FIB-4 in HBeAg-negative treatment-naive chronic hepatitis B patients with significant liver histological lesions receiving 5-year entecavir therapy
In this study, we aimed to evaluate changes in liver fibrosis during 5 years of entecavir (ETV) treatment using noninvasive fibrosis markers in hepatitis B e-antigen (HBeAg)-negative treatment-naive CHB patients who require antiviral therapy. A total of 303 HBeAg-negative treatment-naive patients were enrolled in this study. Liver biopsy was performed before initiati on of antiviral therapy. The diagnosis of CHB was made according to Chinese guidelines for the management of CHB. Patients requiring antiviral therapy (liver fibrosis stage ≥ F2, METAVIR scoring system) were treated with ETV for at least...
Source: Clinical and Experimental Medicine - May 20, 2019 Category: Research Source Type: research
Circulating soluble levels of MIF in women with breast cancer in the molecular subtypes: relationship with Th17 cytokine profile
AbstractBreast cancer (BC) is a health problem worldwide; there is evidence that inflammatory cytokines are increased in BC. Macrophage migration inhibitory factor (MIF) has multiple effects on immune cells, inflammation and cancer. Besides, in previous studies, contradictory and uncertain results have been presented on the implication of Th17 cytokine profile in BC. The aim of this study was to evaluate the plasma levels of MIF and the Th17 cytokine profile in BC and their association with their molecular subtypes and clinical stage. A total of 150 women with BC of Ella Binational Breast Cancer Study and 60 healthy women ...
Source: Clinical and Experimental Medicine - May 18, 2019 Category: Research Source Type: research
Anti-domain 1 of beta2-glycoprotein I aids risk stratification in lupus anticoagulant-positive patients
In conclusion, anti-D1, based on good consistency with triple positivity i n LA-positive patients, has a stronger association with TE and APOs and, to some degree, could predict pregnancy outcomes. Therefore, anti-D1 may aid risk stratification in LA-positive patients. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - May 15, 2019 Category: Research Source Type: research
Adeno-associated virus as a gene therapy vector: strategies to neutralize the neutralizing antibodies
AbstractAdeno-associated virus (AAV)-derived vectors are currently the most common type of viral vectors used in gene therapy clinical trials. The presence of neutralizing antibodies (NAbs) against wild-type AAVs in the host body is one of the limitations for the successful use of AAV vectors. AAV capsid manipulation, by which recombinant vectors lose their ability to interact with NAbs, can help overcome this obstacle. Various methods can be used for this purpose, including directed evolution as well as conjugation of certain chemical groups to AAV epitopes. The present review concisely explains the use of AAV vectors in ...
Source: Clinical and Experimental Medicine - May 3, 2019 Category: Research Source Type: research
TNF- β +252 A & gt;G (rs909253) polymorphism is independently associated with presence of autoantibodies in rheumatoid arthritis patients
AbstractTheTNF- β +252 A>G (rs909253) polymorphism has been associated with a risk of development of rheumatoid arthritis (RA) and could influence plasma tumor necrosis factor alpha (TNF- α) levels. The aim of the present study was to evaluate the association between theTNF- β +252 A>G polymorphism with plasma TNF- α levels, the presence of autoantibodies, and the susceptibility for RA. This cross-sectional study included 261 patients with RA and 292 controls. The polymorphism was studied using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). Soluble TNF...
Source: Clinical and Experimental Medicine - May 2, 2019 Category: Research Source Type: research
Mutation analysis of POLE gene in patients with early-onset colorectal cancer revealed a rare silent variant within the endonuclease domain with potential effect on splicing
AbstractThe colorectal cancer harbor germline, somatic or epimutations in mismatch repair genes,MUTYH orPOLE gene, which lead to the hypermutated and ultramutator phenotypes with increased immune response. The mutations inPOLE gene were reported to occur more frequently in early-onset colorectal cancer (EOCRC), and the patients are strong candidates for checkpoint inhibitor therapy. Here, we report mutation analysis within the endonuclease domain of thePOLE gene in the cohort of patients with EOCRC in order to identify recurrent or new mutations and evaluate their association with the presence of tumor-infiltrating lymphoc...
Source: Clinical and Experimental Medicine - May 2, 2019 Category: Research Source Type: research
Expression of tetraspanins NET-6 and CD151 in breast cancer as a potential tumor biomarker
AbstractTetraspanins have been implicated in multiple biological functions including protein networking and cell signaling. NET-6 (TSPAN 13) has been demonstrated to be a tumor suppressor gene in breast cancer, while CD151 is more likely to act as an oncogene. However, the biological function of both proteins is still inconclusive. Immunohistochemistry was used to analyze the expression of NET-6 and CD151 proteins in breast tumors and benign epithelial cells. The cellular expression of both markers was correlated with HER2, ER, and PR status as well as tumor grade, Ki-67 scores, invasion, and metastasis. Expression of NET-...
Source: Clinical and Experimental Medicine - April 20, 2019 Category: Research Source Type: research
Iloprost use and medical management of systemic sclerosis-related vasculopathy in Italian tertiary referral centers: results from the PROSIT study
AbstractVasculopathy is a crucial feature of systemic sclerosis (SSc), and Raynaud ’s phenomenon (RP) and digital ulcers (DU) have a deep impact on the quality of patients’ life. The management of vascular disease can be challenging for the clinician because of the suboptimal tolerability of the treatments and lack of consensus on the best therapeutic approach. Intravenous ilo prost, a synthetic analogue of prostacyclin, is broadly used for the treatment of RP and ischemic ulcers secondary to SSc. However, no standardized protocol on iloprost use is currently available and, consequently, the management of this ...
Source: Clinical and Experimental Medicine - April 15, 2019 Category: Research Source Type: research
The value of four imaging modalities in diagnosing lymph node involvement in rectal cancer: an overview and adjusted indirect comparison
AbstractSeveral systematic reviews have investigated the accuracy of imaging modalities for lymph node involvement of rectal cancer, but there are considerable differences in conclusions. This overview aimed to assess the methodological and reporting quality of systematic reviews that evaluated the diagnostic value of imaging modalities for lymph node involvement in patients with rectal cancer and to compare the diagnostic value of different modalities for lymph node involvement. The PubMed, EMBASE, Cochrane Library and Chinese Biomedicine Literature were searched to identify relevant systematic reviews. The methodological...
Source: Clinical and Experimental Medicine - March 21, 2019 Category: Research Source Type: research
1q23.1 homozygous deletion and downregulation of Fc receptor-like family genes confer poor prognosis in chronic lymphocytic leukemia
AbstractThe identification of chromosome 1 translocations and deletions is a rare and poorly investigated event in chronic lymphocytic leukemia (CLL). Nevertheless, the identification of novel additional molecular alterations is of great interest, opening to new prognostic and therapeutic strategies for such heterogeneous hematological disease. We here describe a patient affected by CLL with a mutatedIGHV status, showing a balanced t(1;3)(q23.1;q21.3) translocation and a der(18)t(1;18)(q24.2;p11.32), accompanying the recurrent 13q14 heterozygous deletion in all analyzed cells at onset. By combining whole-genome sequencing,...
Source: Clinical and Experimental Medicine - March 15, 2019 Category: Research Source Type: research
Microcystin-LR in peripheral circulation worsens the prognosis partly through oxidative stress in patients with hepatocellular carcinoma
AbstractPrognostic significance of serum microcystin in hepatocellular carcinoma has not been well investigated. The aim of the study was to reveal the relationship between serum microcystin-LR and prognosis in these patients. There were 650 early-stage hepatitis B-induced hepatocellular carcinoma patients, who were not affected by hepatitis C, cirrhosis, heavy drinking or excessive aflatoxin exposure. All of them underwent hepatectomy and were followed up for 5 years. Tumor relapse and overall death were recorded. Blood specimens were collected on admission and at the time of relapse. Serum levels of microcystin-LR ...
Source: Clinical and Experimental Medicine - March 12, 2019 Category: Research Source Type: research
Comparison between clinical significance of serum CXCL-8 and classical tumor markers in oesophageal cancer (OC) patients
AbstractC-X-C motif chemokine 8 (CXCL-8), known as interleukin-8, is a pro-inflammatory cytokine which acts as a chemotactic factor, mainly for leukocytes. CXCL-8 is produced by malignant cells, and therefore it can stimulate the growth and progression of various neoplasms, including oesophageal cancer (OC). The aim of the current study was to measure serum concentrations of chemokine CXCL-8 in OC patients and establish whether this protein might be considered a potential candidate for a tumor marker in the diagnosis and progression of OC. The study included 50 OC subjects (32 patients with squamous cell carcinoma of oesop...
Source: Clinical and Experimental Medicine - February 28, 2019 Category: Research Source Type: research
High BAFF expression associated with active disease in systemic lupus erythematosus and relationship with rs9514828C & gt;T polymorphism in TNFSF13B gene
In this study, the relationship ofTNFSF13B gene rs9514827T>C, rs1041567T>A and rs9514828C>T polymorphisms, mRNA expression and soluble BAFF levels was investigated in 175 SLE patients and 208 healthy controls (HC). TheTNFSF13B polymorphisms were evaluated by PCR –RFLP technique. TheTNFSF13B gene expression was quantified through the RT-PCR assays. The soluble BAFF (sBAFF) levels were measured with ELISA test. There were no differences in genotype and allele frequencies for the threeTNFSF13B polymorphisms, between SLE patients and HC. SLE patients showed 3.15-fold moreTNFSF13B gene expression than HC. The pat...
Source: Clinical and Experimental Medicine - February 11, 2019 Category: Research Source Type: research
Prevalence and characteristics of arthritis in Kawasaki disease: a Chinese cohort study
AbstractArthritis is a major complication of Kawasaki disease (KD). The aims of this study were to define the frequency and the clinical characteristics of arthritis in KD in China and to analyze the relation between arthritis and coronary outcome in KD. We included 1420 KD patients followed at Jiangxi Children ’s Hospital from January 2014 to December 2017. Demographic, clinical and laboratory features of KD were analyzed. Among the 1420 patients enrolled, 151 had arthritis. The median age of KD patients with arthritis was 29 months and older than those without arthritis (20 months). Of the 151 patien ts d...
Source: Clinical and Experimental Medicine - February 6, 2019 Category: Research Source Type: research
TRAF6 regulates YAP signaling by promoting the ubiquitination and degradation of MST1 in pancreatic cancer
AbstractTNF receptor-associated factor 6 (TRAF6), a regulator of NF- κB signaling, has been reported to be associated with the oncogenesis of various tumors including pancreatic cancer, but the underlying mechanisms remain unknown. Here, we found that knocking down the expression of TRAF6 impaired YAP signaling. Moreover, TRAF6 promoted the migration and colony form ation of pancreatic cancer cells through YAP. Then, we found that TRAF6 interacted with and promoted the ubiquitination and degradation of MST1, and the expression of TRAF6 and MST1 was negatively correlated in primary human pancreatic cancer samples. Our...
Source: Clinical and Experimental Medicine - January 23, 2019 Category: Research Source Type: research
Serum miR-122 levels correlate with diabetic retinopathy
AbstractDiabetic retinopathy is the most severe ocular complication of diabetes and may lead to visual disability and blindness. Proliferative diabetic retinopathy (PDR) is characterized by ischemia-induced neovascularization with associated complications. An association was established between the presence of PDR, cardiovascular disease, and mortality among patients with type 1 diabetes mellitus and type 2 diabetes mellitus in epidemiological studies. However, the mechanism underlying increased cardiovascular risk in patients with PDR is still unknown. In recent years, a group of miRNAs has been linked to the pathology of...
Source: Clinical and Experimental Medicine - January 23, 2019 Category: Research Source Type: research
Droplet digital PCR revealed high concordance between primary tumors and lymph node metastases in multiplex screening of KRAS mutations in colorectal cancer
In this study, we report the application of ddPCR technology in order to analyze the presence ofKRAS mutations in primary tumor and matched metastasis in lymph nodes (LNs) from patients with mCRC and address the question, whether the improvement in the detection method can lower the discrepancies ofKRAS mutations detection between the primary tumor and regional LNs. Genomic DNA with wtKRAS and commercial DNA with mtKRAS (G12D) were used to set up the ddPCR reaction. Formalin-fixed paraffin-embedded tissues from primary tumor and positive lymph node from 31 patients with mCRC were analyzed using ddPCR and Sanger sequencing....
Source: Clinical and Experimental Medicine - January 19, 2019 Category: Research Source Type: research
Interleukin-6 is prone to be a candidate biomarker for predicting incomplete and IVIG nonresponsive Kawasaki disease rather than coronary artery aneurysm
AbstractKawasaki disease (KD) is an acute, systemic vasculitis and occurs mainly in childhood. Interleukin-6 (IL-6) is a pleiotropic cytokine synthesized predominantly by neutrophils and monocytes/macrophages and plays an important role in systemic inflammatory disease. However, a little information is currently available on the relationship of serum IL-6 with conventional inflammatory mediators, clinical classification, IVIG response and coronary artery aneurysm (CAA). 165 Chinese children with KD were enrolled and divided into six subgroups, including complete KD, incomplete KD, IVIG-responsive KD, IVIG-nonresponsive KD,...
Source: Clinical and Experimental Medicine - January 8, 2019 Category: Research Source Type: research
Effect of antithyroid drugs on the occurrence of antibodies against type 2 deiodinase (DIO2), which are involved in hyperthyroid Graves ’ disease influencing the therapeutic efficacy
AbstractGraves ’ disease is an organ-specific autoimmune disease with hyperthyroidism, diffuse goiter and autoantibodies against TSH receptor, thyroid peroxidase (TPO) and/or thyroglobulin (Tg). Graves’ hyperthyroidism is characterized by T3 dominance due to the conversion of T4 into T3 through type 1 and 2 deiodinase enzymes (DIO1, DIO2). Methimazole (MMI) and propylthiouracil (PTU) therapies inhibit thyroid hormone synthesis blocking the activity of deiodinase and TPO enzymes. The study investigated the occurrence of autoantibodies against DIO2 peptides (cys- and hom-peptides) with the effect of antithyroid d...
Source: Clinical and Experimental Medicine - January 4, 2019 Category: Research Source Type: research
PLX9486 shows anti-tumor efficacy in patient-derived, tyrosine kinase inhibitor-resistant KIT-mutant xenograft models of gastrointestinal stromal tumors
In conclusion, PLX9486 showed anti-tumor efficacy in patient-derived imatinib-resistant GIST xenograft models, mainly through inhibition of KIT signaling. These preclinical efficacy data encourage further testing of PLX9486 in the clinical setting. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - December 6, 2018 Category: Research Source Type: research
Meta-analysis of FOLFIRINOX regimen as the first-line chemotherapy for locally advanced pancreatic cancer and borderline resectable pancreatic cancer
AbstractThe study aimed to evaluate the effectiveness of the first-line chemotherapy FOLFIRINOX in treating pancreatic cancer. Pertinent studies were derived from the PubMed, Cochrane Library and EMBASE. The outcomes were analyzed according to resection rate and radical (R0) resection rate. Data were expressed as weighted commix proportions with 95% confidence intervals (CIs). Twenty-three studies, involving 968 patients with locally advanced pancreatic cancer (LAPC) and borderline resectable pancreatic cancer (BRPC), were examined. After treatment, 55% (95% CI 52 –58%) of the patients underwent resection and 40% (95...
Source: Clinical and Experimental Medicine - November 29, 2018 Category: Research Source Type: research
FDG PET/CT as a survival prognostic factor in patients with advanced renal cell carcinoma
AbstractAccurate prediction of the outcome of molecular target-based treatment in advanced renal cell carcinoma (RCC) is an important clinical problem. Positron emission tomography/computed tomography using [18F]-2-fluoro-2-deoxyglucose (FDG PET/CT) is a noninvasive tool for the assessment of glucose accumulation which can be a marker of the biological characteristics of the tumor. In this paper, we assess FDG PET/CT as a survival prognostic marker in patients with advanced RCC. The study included 121 patients treated in the years 2011 –2016 with a diagnosis of advanced renal cell carcinoma (stage IV, multifocal meta...
Source: Clinical and Experimental Medicine - November 28, 2018 Category: Research Source Type: research
Associations between the neutrophil-to-lymphocyte and the platelet-to-lymphocyte ratios and the presence and severity of psoriasis: a systematic review and meta-analysis
AbstractThe diagnosis of psoriasis, an immune-mediated disease that affects 2% of the population in Western countries, is largely based on history and clinical examination. The aim of this systematic review and meta-analysis was to investigate the associations between the neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) and the presence and clinical severity of psoriasis. A systematic literature search was conducted in PubMed, Web of Science, and Scopus, from inception to January 2018. Twelve case –control studies enrolling 1067 psoriasis patients (537 males and 530 females) and 799 healthy...
Source: Clinical and Experimental Medicine - November 26, 2018 Category: Research Source Type: research
Serum histamine and acetylcholine variations as new noninvasive biochemical markers in staging of experimental hepatocellular carcinoma
AbstractAngiogenesis is a major prerequisite for hepatocellular carcinoma (HCC) development and progression. The present study aims to assess the potential role of two endogenous regulators of angiogenesis histamine (His) and acetylcholine (Ach), as possible biochemical markers for staging of HCC. Five groups of rats were used in this study: a control healthy group (I), another 4 intoxicated groups used for the induction of HCC with a high dose of diethyl nitrosamine (DENA, 200 mg/kg, single I.P. dose), (II, III, IV, and V). Groups II, III, IV, and V were killed following 8, 16, 24, and 32 weeks after DENA injec...
Source: Clinical and Experimental Medicine - November 20, 2018 Category: Research Source Type: research
Clinical practice: hepatitis C virus infection, cryoglobulinemia and cryoglobulinemic vasculitis
AbstractCryoglobulins are circulating immunoglobulins that reversibly precipitate at temperatures below 37 °C. Type-II cryoglobulins consist of monoclonal IgM/polyclonal IgG immune complexes (ICs), whereas in type-III cryoglobulins both IgM and IgG are polyclonal. The clinical condition resulting from the presence of cryoglobulins in the blood is called mixed cryoglobulinemia (MC), which can be asympt omatic or manifest as cryoglobulinemic vasculitis (CV). Type-I cryoglobulins, consisting of a single monoclonal isotype, are detected in patients with lymphoproliferative disorders. It is now established that> &t...
Source: Clinical and Experimental Medicine - November 14, 2018 Category: Research Source Type: research
The risk of systemic lupus erythematosus associated with Epstein –Barr virus infection: a systematic review and meta-analysis
We examined the association between EBV and SLE patients by conducting a systematic review and meta-analysis of case –control studies that examined the prevalence of EBV antibodies and the DNA-positive rate. We searched the MEDLINE and EMBASE databases from 1966 to 2018 with no language restrictions. The Mantel–Haenszel odds ratios (OR) for EBV antibody sero-positivity were calculated, and meta-analyses were c onducted. Quality assessment was performed using a modified version of the Newcastle–Ottawa scale, and 33 studies were included. Most studies found a higher sero-prevalence of VCA IgG and EA IgG in ...
Source: Clinical and Experimental Medicine - October 25, 2018 Category: Research Source Type: research
Myeloid cell-like transcript 2 is related to liver inflammation and the pathogenesis of hepatitis B via the involvement of CD8 + T cell activation
This study analysed the biological significance of TLT-2 on CD8+T cells in hepatitis B patients and provided a theoretical basis for the potential role of TLT-2 as an immune regulator. Flow cytometry sorting, isobaric tags for relative and absolute quantitation and short hairpin RNAs were used to analyse the function of TLT-2 on CD8+T cells in hepatitis B patients. The TLT-2 expression levels in the acute hepatitis B and chronic hepatitis B groups were significantly higher than that in the healthy control group and were positively correlated with ALT and AST. The CD8+TLT-2+T cells exhibited stronger immune function and gre...
Source: Clinical and Experimental Medicine - October 25, 2018 Category: Research Source Type: research
Myostatin and other musculoskeletal markers in lung transplant recipients
AbstractRecipients of lung transplantation (LuTx) may experience impaired muscle function and bone metabolism even after rehabilitation. We investigated the potential use of musculoskeletal markers in identifying the impairment of muscle function and bone function in these patients. Biochemical parameters, bodily functions, and lung function of 37 LuTx recipients were evaluated at the time of their discharge from the hospital stay and about 6 months later. The biomarkers were also assessed in 30 healthy age and gender distribution-matched controls. Compared to controls, the negative muscle regulator myostatin was ele...
Source: Clinical and Experimental Medicine - October 13, 2018 Category: Research Source Type: research
mTOR and ERK regulate VKORC1 expression in both hepatoma cells and hepatocytes which influence blood coagulation
AbstractDeficiency of γ-glutamyl carboxylation of coagulation factors, as evidenced by the elevated level of Des-γ-carboxyl prothrombin (DCP), is a common feature in hepatocellular carcinoma patients. Additionally, treatment of cancer patients with mTOR inhibitors significantly increases hemorrhagic events. However, th e underlying mechanisms remain unknown. In the present study, Vitamin K epoxide reductase complex subunit 1 (VKORC1) was found to be significantly down-regulated in clinical hepatoma tissues and most tested hepatoma cell lines. In vitro investigations showed that VKORC1 expression was promoted by...
Source: Clinical and Experimental Medicine - October 10, 2018 Category: Research Source Type: research
RANKL-induced c-Src activation contributes to conventional anti-cancer drug resistance and dasatinib overcomes this resistance in RANK-expressing multiple myeloma cells
AbstractThe survival and growth of multiple myeloma (MM) cells are facilitated by cell –cell interactions with bone marrow stromal cells and the bone marrow microenvironment. These interactions induce de novo drug resistance known as cell adhesion-mediated drug resistance. Our previous results recently revealed that the receptor activator of NF-κB (RANK) ligand (RANKL), which is ex pressed by bone marrow stromal cells, contributes to anti-cancer drug resistance through the activation of various signaling molecules and suppression of Bim expression in RANK-expressing MM cells. However, the detailed mechanisms un...
Source: Clinical and Experimental Medicine - October 5, 2018 Category: Research Source Type: research
Imbalance of circulating Tfr/Tfh ratio in patients with rheumatoid arthritis
AbstractFollicular helper T(Tfh) cells and follicular regulatory T(Tfr) cells are critical for the development and maintenance of germinal center and humoral immune responses. Accumulating evidence has demonstrated that the dysregulation of either Tfh or Tfr cells contributes to the pathogenesis of autoimmune diseases. The aim of this study was to examine the numbers of Tfh and Tfr cells in patients with rheumatoid arthritis (RA). Twenty-four patients with RA patients and 20 health controls (HCs) were enrolled in this study. We analyzed the numbers of Tfh (CD4+ CXCR5+ PD-1hi) cells and Tfr (CD4+ CXCR5+CD127lo) cells in 24 ...
Source: Clinical and Experimental Medicine - October 4, 2018 Category: Research Source Type: research
Association of the CYP4V2 polymorphism rs13146272 with venous thromboembolism in a Chinese population
In this study, 226 VTE patients and 205 healthy controls were recruited, and the allele frequency of variant rs13146272 was analysed by a MassARRA Y SNP genotyping assay. In addition, 9 case–control cohorts from 5 studies involving 6667 VTE-affected individuals and 8716 control subjects were included in this meta-analysis. Pooled ORs and 95% CIs were calculated to assess the association between rs13146272 and VTE by using different genetic m odels. Our case–control study results showed that there was no significant association between VTE and rs13146272 under the additive model (OR = 0.92, 95% CIs...
Source: Clinical and Experimental Medicine - October 1, 2018 Category: Research Source Type: research
Association between TLR4 polymorphisms (896 A & gt;G, 1196 C & gt;T, − 2570 A & gt;G, − 2081 G & gt;A) and virulence factors in uropathogenic Escherichia coli
This study includes 100 samples from patients diagnosed with UTI and 100 samples from uninfected subjects. A conventional urine culture was performed and the isolates were identified by using the Vitek automated system.TLR4 gene polymorphisms were identified by the PCR –RFLP technique. ThehlyA, fimH, papC, iutA andcnf1 virulence factors as well as theE. coli phylogenetic group were assessed by PCR. In this study, it was observed that the presence of the − 2570 polymorphism represents a risk of UTI (p T polymorphisms were associated with theE. coli virulence factorsfimH andhlyA, respectively (p
Source: Clinical and Experimental Medicine - September 15, 2018 Category: Research Source Type: research
Ex vivo modelling of the formation of inflammatory platelet-leucocyte aggregates and their adhesion on endothelial cells, an early event in sepsis
In conclusion, we have dev eloped an in vitro method that models the acute inflammatory reaction in whole blood in the presence of sepsis-relevant bacterial pathogen surfaces. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - September 6, 2018 Category: Research Source Type: research
Correction to: Association between IL-35 and coronary arterial lesions in children with Kawasaki disease
The original version of this article unfortunately contained a mistake. The copyright line was incorrect in the HTML version. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - August 31, 2018 Category: Research Source Type: research
Massive stent thrombosis during active ulcerative colitis: the tricky balance between manifest hemorrhagic and concealed thrombotic risk
We report the case of a 70-year-old man who presented with ST elevation myocardial infarction during the active phase of ulcerative colitis (UC). Because of the ongoing hematochezia, after successful revascularization of the culprit vessel, the patient was medicated with Clopidogrel, in place of one of the more powerful new oral P2Y12 inhibitors that currently represent the gold standard therapy. Few days later a second elective percutaneous coronary intervention (PCI) on a non-culprit vessel ensued in a life-threatening early massive stent thrombosis involving the left main. During and after emergency PCI antiplatelet the...
Source: Clinical and Experimental Medicine - August 22, 2018 Category: Research Source Type: research
Upregulation of miR-221/222 expression in rheumatoid arthritis (RA) patients: correlation with disease activity
AbstractmiRNAs are noncoding RNA that play a critical role as fine regulators of gene expression at the posttranscriptional level within cells in numerous autoimmune diseases. miR-221/222 play a role in cancer by regulating cell proliferation, invasion and apoptosis. However, there have been insufficient studies on their role in rheumatoid arthritis (RA). This work is designed to analyze the miR-221/222 expression patterns in peripheral blood mononuclear cells (PBMCs) of patients with RA in comparison with healthy controls using quantitative RT-PCR, in a group of 30 RA patients and 20 healthy controls. The fold change of m...
Source: Clinical and Experimental Medicine - August 21, 2018 Category: Research Source Type: research
Establishment and evaluation of a general dissociation technique for antibodies in circulating immune complexes
This study aimed to establish a general and efficient dissociation technique for detecting antibodies in circulating immune complexes (CICs) in serum and to evaluate its clinical application. CICs were efficiently separated from specimens using polyethylene glycol double-precipitation. The best conditions for anti-HBs dissociation from HBsAg-ICs were a pH of 1.80, incubation at 15 °C for 5–10 min, and detection within 10 min after neutralization. The mean dissociation rate, reproducibility, mean dissociation recovery rate and specificity of the new technique were 64.3%, 12 mon...
Source: Clinical and Experimental Medicine - August 17, 2018 Category: Research Source Type: research
The role of the alternative pathway of complement activation in glomerular diseases
AbstractThe complement system (CS) has recently been recognized as a bridge between innate and adaptive immunity that constitutes a very complex mechanism controlling the clearance of pathogens, cellular debris, and immune complexes. Out of three known pathways of complement activation, the alternative pathway (AP) plays a critical role in host defense by amplifying the complement response, independently of initiation pathway and continuously maintaining low-level activity in a process called ‘thick-over.’ A key molecule of the CS is C3, in which the AP is constantly activated. To prevent host cell destruction,...
Source: Clinical and Experimental Medicine - August 1, 2018 Category: Research Source Type: research