Linear dichroism reveals the perpendicular orientation of DNA bases in the RecA and Rad51 recombinase filaments: A possible mechanism for the strand exchange reaction
Chirality. 2024 Apr;36(4):e23664. doi: 10.1002/chir.23664.ABSTRACTLinear dichroism spectroscopy is used to investigate the structure of RecA family recombinase filaments (RecA and Rad51 proteins) with DNA for clarifying the molecular mechanism of DNA strand exchange promoted by these proteins and its activation. The measurements show that the recombinases promote the perpendicular base orientation of single-stranded DNA only in the presence of activators, indicating the importance of base orientation in the reaction. We summarize the results and discuss the role of DNA base orientation.PMID:38561319 | DOI:10.1002/chir.2366...
Source: Chirality - April 1, 2024 Category: Molecular Biology Authors: Masayuki Takahashi Kentaro Ito Hiroshi Iwasaki Bengt Norden Source Type: research
Separation of enantiomers of chiral fullerene derivatives through enantioselective encapsulation within an adaptable helical cavity of syndiotactic poly(methyl methacrylate) with helicity memory
Chirality. 2024 Apr;36(4):e23663. doi: 10.1002/chir.23663.ABSTRACTOptically active left (M)- and right (P)-handed helical syndiotactic poly(methyl methacrylate)s (M- and P-st-PMMAs) with a helicity memory enantioselectively encapsulated the racemic C60 derivatives, such as 3,4-fulleroproline tert-butyl ester (rac-1) and tetraallylated C60 (rac-2), as well as the C60-bound racemic 310-helical peptides (rac-3) within their helical cavities to form peapod-like inclusion complexes and a unique "helix-in-helix" superstructure, respectively. The enantiomeric excess (ee) and separation factor (enantioselectivity) (α) of the anal...
Source: Chirality - April 1, 2024 Category: Molecular Biology Authors: Daisuke Taura Akiko Minami Fumihiko Mamiya Naoki Ousaka Kenichiro Itami Eiji Yashima Source Type: research
Linear dichroism reveals the perpendicular orientation of DNA bases in the RecA and Rad51 recombinase filaments: A possible mechanism for the strand exchange reaction
Chirality. 2024 Apr;36(4):e23664. doi: 10.1002/chir.23664.ABSTRACTLinear dichroism spectroscopy is used to investigate the structure of RecA family recombinase filaments (RecA and Rad51 proteins) with DNA for clarifying the molecular mechanism of DNA strand exchange promoted by these proteins and its activation. The measurements show that the recombinases promote the perpendicular base orientation of single-stranded DNA only in the presence of activators, indicating the importance of base orientation in the reaction. We summarize the results and discuss the role of DNA base orientation.PMID:38561319 | DOI:10.1002/chir.2366...
Source: Chirality - April 1, 2024 Category: Molecular Biology Authors: Masayuki Takahashi Kentaro Ito Hiroshi Iwasaki Bengt Norden Source Type: research
Separation of enantiomers of chiral fullerene derivatives through enantioselective encapsulation within an adaptable helical cavity of syndiotactic poly(methyl methacrylate) with helicity memory
Chirality. 2024 Apr;36(4):e23663. doi: 10.1002/chir.23663.ABSTRACTOptically active left (M)- and right (P)-handed helical syndiotactic poly(methyl methacrylate)s (M- and P-st-PMMAs) with a helicity memory enantioselectively encapsulated the racemic C60 derivatives, such as 3,4-fulleroproline tert-butyl ester (rac-1) and tetraallylated C60 (rac-2), as well as the C60-bound racemic 310-helical peptides (rac-3) within their helical cavities to form peapod-like inclusion complexes and a unique "helix-in-helix" superstructure, respectively. The enantiomeric excess (ee) and separation factor (enantioselectivity) (α) of the anal...
Source: Chirality - April 1, 2024 Category: Molecular Biology Authors: Daisuke Taura Akiko Minami Fumihiko Mamiya Naoki Ousaka Kenichiro Itami Eiji Yashima Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Biocatalytic enantioselective synthesis of cenobamate, an antiepileptic drug
Chirality. 2024 Apr;36(4):e23660. doi: 10.1002/chir.23660.ABSTRACTA green and efficient process for the synthesis of cenobamate has been accomplished in 70% yield and >99% ee through the bio-reduction of β-ketotetrazole using Daucus carota whole plant cells. The corresponding β-hydroxytetrazole was isolated in 60% yield and >98% ee. This is the first report on the biocatalytic reduction of β-ketotetrazole using plant enzymes derived from D. carota root cells with excellent enantioselectivity.PMID:38511944 | DOI:10.1002/chir.23660 (Source: Chirality)
Source: Chirality - March 21, 2024 Category: Molecular Biology Authors: Vennapusa Haritha Pulivarthi Deepthi Rachel Gundamalla Kommu Nagesh Suggala V Satyanarayana Adari Bhaskar Rao Sridhar Balasubramanian Basireddy Venkata Subba Reddy Source Type: research
Simultaneous identification and enantioseparation of ofloxacin and duloxetine without the single standard and computational calculation of their inclusion complexes
Chirality. 2024 Mar;36(3):e23661. doi: 10.1002/chir.23661.ABSTRACTGiven the markedly different pharmacological activities between enantiomeric isomers, it is crucial to encourage the stereoselective determination of chiral drugs in the biological and pharmaceutical fields, and the combination of drugs makes this analysis more complicated and challenging. Herein, a capillary electrophoresis (CE) method for the enantioseparation of ofloxacin and duloxetine was established, enabling the simultaneous identification of four isomers in nonracemic mixtures with enantiomeric excess (ee%) values exceeding 5%. This was achieved thro...
Source: Chirality - March 8, 2024 Category: Molecular Biology Authors: Lixia Chen Xiangjun Li Source Type: research
Simultaneous identification and enantioseparation of ofloxacin and duloxetine without the single standard and computational calculation of their inclusion complexes
Chirality. 2024 Mar;36(3):e23661. doi: 10.1002/chir.23661.ABSTRACTGiven the markedly different pharmacological activities between enantiomeric isomers, it is crucial to encourage the stereoselective determination of chiral drugs in the biological and pharmaceutical fields, and the combination of drugs makes this analysis more complicated and challenging. Herein, a capillary electrophoresis (CE) method for the enantioseparation of ofloxacin and duloxetine was established, enabling the simultaneous identification of four isomers in nonracemic mixtures with enantiomeric excess (ee%) values exceeding 5%. This was achieved thro...
Source: Chirality - March 8, 2024 Category: Molecular Biology Authors: Lixia Chen Xiangjun Li Source Type: research
