Circular dichroism and UV-Vis detection of UV-induced damage to nucleic acids
In this report, we demonstrate that CD spectroscopy can be used as a tool to detect changes to DNA upon irradiation with UV light. We follow the spectroscopic response of DNA samples irradiated at selected exposure times with both CD and UV-Vis spectroscopy. We analyzed four different nucleic acids to evaluate the effect of the sequence on photodegradation. Only one polymer, calf thymus DNA, was a natural nucleic acid and contained all four nucleobases. The other three were synthetic polymers and contained only one type of base pair: poly (deoxyadenylic-deoxythymidylic) acid [poly (dA-dT)2 ] and poly (deoxyadenylic acid) Â...
Source: Chirality - July 28, 2023 Category: Molecular Biology Authors: Reed C Dowling Gregory T Carroll David L Kirschman Mark B Masthay Angela Mammana Source Type: research
Circular dichroism and UV-Vis detection of UV-induced damage to nucleic acids
In this report, we demonstrate that CD spectroscopy can be used as a tool to detect changes to DNA upon irradiation with UV light. We follow the spectroscopic response of DNA samples irradiated at selected exposure times with both CD and UV-Vis spectroscopy. We analyzed four different nucleic acids to evaluate the effect of the sequence on photodegradation. Only one polymer, calf thymus DNA, was a natural nucleic acid and contained all four nucleobases. The other three were synthetic polymers and contained only one type of base pair: poly (deoxyadenylic-deoxythymidylic) acid [poly (dA-dT)2 ] and poly (deoxyadenylic acid) Â...
Source: Chirality - July 28, 2023 Category: Molecular Biology Authors: Reed C Dowling Gregory T Carroll David L Kirschman Mark B Masthay Angela Mammana Source Type: research
Circular dichroism and UV-Vis detection of UV-induced damage to nucleic acids
In this report, we demonstrate that CD spectroscopy can be used as a tool to detect changes to DNA upon irradiation with UV light. We follow the spectroscopic response of DNA samples irradiated at selected exposure times with both CD and UV-Vis spectroscopy. We analyzed four different nucleic acids to evaluate the effect of the sequence on photodegradation. Only one polymer, calf thymus DNA, was a natural nucleic acid and contained all four nucleobases. The other three were synthetic polymers and contained only one type of base pair: poly (deoxyadenylic-deoxythymidylic) acid [poly (dA-dT)2 ] and poly (deoxyadenylic acid) Â...
Source: Chirality - July 28, 2023 Category: Molecular Biology Authors: Reed C Dowling Gregory T Carroll David L Kirschman Mark B Masthay Angela Mammana Source Type: research
Circular dichroism and UV-Vis detection of UV-induced damage to nucleic acids
In this report, we demonstrate that CD spectroscopy can be used as a tool to detect changes to DNA upon irradiation with UV light. We follow the spectroscopic response of DNA samples irradiated at selected exposure times with both CD and UV-Vis spectroscopy. We analyzed four different nucleic acids to evaluate the effect of the sequence on photodegradation. Only one polymer, calf thymus DNA, was a natural nucleic acid and contained all four nucleobases. The other three were synthetic polymers and contained only one type of base pair: poly (deoxyadenylic-deoxythymidylic) acid [poly (dA-dT)2 ] and poly (deoxyadenylic acid) Â...
Source: Chirality - July 28, 2023 Category: Molecular Biology Authors: Reed C Dowling Gregory T Carroll David L Kirschman Mark B Masthay Angela Mammana Source Type: research
Circular dichroism and UV-Vis detection of UV-induced damage to nucleic acids
In this report, we demonstrate that CD spectroscopy can be used as a tool to detect changes to DNA upon irradiation with UV light. We follow the spectroscopic response of DNA samples irradiated at selected exposure times with both CD and UV-Vis spectroscopy. We analyzed four different nucleic acids to evaluate the effect of the sequence on photodegradation. Only one polymer, calf thymus DNA, was a natural nucleic acid and contained all four nucleobases. The other three were synthetic polymers and contained only one type of base pair: poly (deoxyadenylic-deoxythymidylic) acid [poly (dA-dT)2 ] and poly (deoxyadenylic acid) Â...
Source: Chirality - July 28, 2023 Category: Molecular Biology Authors: Reed C Dowling Gregory T Carroll David L Kirschman Mark B Masthay Angela Mammana Source Type: research
Circular dichroism and UV-Vis detection of UV-induced damage to nucleic acids
In this report, we demonstrate that CD spectroscopy can be used as a tool to detect changes to DNA upon irradiation with UV light. We follow the spectroscopic response of DNA samples irradiated at selected exposure times with both CD and UV-Vis spectroscopy. We analyzed four different nucleic acids to evaluate the effect of the sequence on photodegradation. Only one polymer, calf thymus DNA, was a natural nucleic acid and contained all four nucleobases. The other three were synthetic polymers and contained only one type of base pair: poly (deoxyadenylic-deoxythymidylic) acid [poly (dA-dT)2 ] and poly (deoxyadenylic acid) Â...
Source: Chirality - July 28, 2023 Category: Molecular Biology Authors: Reed C Dowling Gregory T Carroll David L Kirschman Mark B Masthay Angela Mammana Source Type: research
Circular dichroism and UV-Vis detection of UV-induced damage to nucleic acids
In this report, we demonstrate that CD spectroscopy can be used as a tool to detect changes to DNA upon irradiation with UV light. We follow the spectroscopic response of DNA samples irradiated at selected exposure times with both CD and UV-Vis spectroscopy. We analyzed four different nucleic acids to evaluate the effect of the sequence on photodegradation. Only one polymer, calf thymus DNA, was a natural nucleic acid and contained all four nucleobases. The other three were synthetic polymers and contained only one type of base pair: poly (deoxyadenylic-deoxythymidylic) acid [poly (dA-dT)2 ] and poly (deoxyadenylic acid) Â...
Source: Chirality - July 28, 2023 Category: Molecular Biology Authors: Reed C Dowling Gregory T Carroll David L Kirschman Mark B Masthay Angela Mammana Source Type: research
Straightforward synthesis of chiral sulfonate-based ionic liquids from amino alcohols for chiral recognition
Chirality. 2023 Jul 27. doi: 10.1002/chir.23614. Online ahead of print.ABSTRACTNew sulfonate-based chiral salts were prepared from amino alcohols and sodium hydroxymethanesulfonate, vinyl sulfonate, or sultone. The synthesis started with different amino acids from the chiral pool and gave the desired products in just four steps. After cation metathesis, the salts were explored as chiral solvating agents (CSAs) in NMR studies. The new chiral ionic liquids (CILs) were successfully able to interact with different chiral guest molecules and formed diastereomeric aggregates. In some cases, baseline separation was observed. The ...
Source: Chirality - July 27, 2023 Category: Molecular Biology Authors: Niklas Feuge Ren é Wilhelm Source Type: research
Resolution by diastereomeric salts: Access to both enantiomers of racemic acid using the same enantiomer of resolving base
Chirality. 2023 Jul 27. doi: 10.1002/chir.23615. Online ahead of print.ABSTRACTRacemic carboxylic acid, a Diels-Alder cycloadduct derived from 5-bromo-3-phenyl-α-pyrone and acrylate dienophile, was resolved into enantiomers by diastereomeric salt crystallization. Quinidine was used as a sole resolving base. The salt of (+)-acid crystallized from aqueous acetonitrile solution. Once this salt was separated by filtration, quinidine salt with (-)-acid crystallized from mother liquor. As a result, both enantiomers of Diels-Alder cycloadduct were isolated in high enantiomeric purity.PMID:37497758 | DOI:10.1002/chir.23615 (Source: Chirality)
Source: Chirality - July 27, 2023 Category: Molecular Biology Authors: Vitaly Kovalenko Ivana C ÃsaÅ™ová Source Type: research
Straightforward synthesis of chiral sulfonate-based ionic liquids from amino alcohols for chiral recognition
Chirality. 2023 Jul 27. doi: 10.1002/chir.23614. Online ahead of print.ABSTRACTNew sulfonate-based chiral salts were prepared from amino alcohols and sodium hydroxymethanesulfonate, vinyl sulfonate, or sultone. The synthesis started with different amino acids from the chiral pool and gave the desired products in just four steps. After cation metathesis, the salts were explored as chiral solvating agents (CSAs) in NMR studies. The new chiral ionic liquids (CILs) were successfully able to interact with different chiral guest molecules and formed diastereomeric aggregates. In some cases, baseline separation was observed. The ...
Source: Chirality - July 27, 2023 Category: Molecular Biology Authors: Niklas Feuge Ren é Wilhelm Source Type: research
Resolution by diastereomeric salts: Access to both enantiomers of racemic acid using the same enantiomer of resolving base
Chirality. 2023 Jul 27. doi: 10.1002/chir.23615. Online ahead of print.ABSTRACTRacemic carboxylic acid, a Diels-Alder cycloadduct derived from 5-bromo-3-phenyl-α-pyrone and acrylate dienophile, was resolved into enantiomers by diastereomeric salt crystallization. Quinidine was used as a sole resolving base. The salt of (+)-acid crystallized from aqueous acetonitrile solution. Once this salt was separated by filtration, quinidine salt with (-)-acid crystallized from mother liquor. As a result, both enantiomers of Diels-Alder cycloadduct were isolated in high enantiomeric purity.PMID:37497758 | DOI:10.1002/chir.23615 (Source: Chirality)
Source: Chirality - July 27, 2023 Category: Molecular Biology Authors: Vitaly Kovalenko Ivana C ÃsaÅ™ová Source Type: research
Stereoselective study of fluoxetine and norfluoxetine across the blood-brain barrier mediated by organic cation transporter 1/3 in rats using an enantioselective UPLC-MS/MS method
In this study, accurate and reliable UPLC-MS/MS enantioselective analysis was established in rat plasma and brain. The characteristics of FLT and NFLT enantiomers across the BBB were studied by chemical knockout of rat transporters. We found that the dominant enantiomers of FLT and NFLT were S-FLT and R-NFLT, respectively, both in plasma and in brain. The FLT and NFLT enantiomers showed significant stereoselectivity across the BBB, and S-FLT and S-NFLT were the dominant configurations across the BBB. Chemical knockout of organic cation transporter 1 (OCT1) and OCT3 can affect the ratio of plasma FLT and NFLT enantiomers in...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Hai An Jinjun Fang Min Wang Haiyang Lin Yingying Sun Bingying Hu Zhisheng He Zhen Ge Yang Wei Source Type: research
Chiral LC-MS/MS method for the discrimination of triiodothyronine enantiomers on a crown ether-based chiral stationary phase
Chirality. 2023 Jul 18. doi: 10.1002/chir.23611. Online ahead of print.ABSTRACTConsidering the greater pharmaceutical and clinical interest of triiodothyronine (T3 ) thyroid hormone, an effective D/L-T3 enantiomer separation was performed on a crown ether-based chiral stationary phase by LC-MS/MS. In optimal analytical condition and selected reaction monitoring mode, the two enantiomers of T3 were baseline separated within 10 min. The limit of detection and limit of quantitation were found to be 0.05 and 0.10 ng/μl; 0.20 and 0.50 ng/μl for D- and L-T3 , respectively. During validation, this method proved to be feasible, ...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Byeongchan Choi Suraj Adhikari Moong-Gi Ji Man-Jeong Paik Gwang Lee Wonjae Lee Source Type: research
Stereoselective study of fluoxetine and norfluoxetine across the blood-brain barrier mediated by organic cation transporter 1/3 in rats using an enantioselective UPLC-MS/MS method
In this study, accurate and reliable UPLC-MS/MS enantioselective analysis was established in rat plasma and brain. The characteristics of FLT and NFLT enantiomers across the BBB were studied by chemical knockout of rat transporters. We found that the dominant enantiomers of FLT and NFLT were S-FLT and R-NFLT, respectively, both in plasma and in brain. The FLT and NFLT enantiomers showed significant stereoselectivity across the BBB, and S-FLT and S-NFLT were the dominant configurations across the BBB. Chemical knockout of organic cation transporter 1 (OCT1) and OCT3 can affect the ratio of plasma FLT and NFLT enantiomers in...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Hai An Jinjun Fang Min Wang Haiyang Lin Yingying Sun Bingying Hu Zhisheng He Zhen Ge Yang Wei Source Type: research
Chiral LC-MS/MS method for the discrimination of triiodothyronine enantiomers on a crown ether-based chiral stationary phase
Chirality. 2023 Jul 18. doi: 10.1002/chir.23611. Online ahead of print.ABSTRACTConsidering the greater pharmaceutical and clinical interest of triiodothyronine (T3 ) thyroid hormone, an effective D/L-T3 enantiomer separation was performed on a crown ether-based chiral stationary phase by LC-MS/MS. In optimal analytical condition and selected reaction monitoring mode, the two enantiomers of T3 were baseline separated within 10 min. The limit of detection and limit of quantitation were found to be 0.05 and 0.10 ng/μl; 0.20 and 0.50 ng/μl for D- and L-T3 , respectively. During validation, this method proved to be feasible, ...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Byeongchan Choi Suraj Adhikari Moong-Gi Ji Man-Jeong Paik Gwang Lee Wonjae Lee Source Type: research
