Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether-derived column
In this study, crown ether-derived column Crownpak® CR-I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide-based columns. Crownpak® CR-I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR-I (+). The first reported preparative SFC separations on Crownpak® CR-I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstr...
Source: Chirality - September 22, 2023 Category: Molecular Biology Authors: Lei Yue Larry Miller John Reilly Source Type: research
Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether-derived column
In this study, crown ether-derived column Crownpak® CR-I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide-based columns. Crownpak® CR-I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR-I (+). The first reported preparative SFC separations on Crownpak® CR-I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstr...
Source: Chirality - September 22, 2023 Category: Molecular Biology Authors: Lei Yue Larry Miller John Reilly Source Type: research
Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether-derived column
In this study, crown ether-derived column Crownpak® CR-I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide-based columns. Crownpak® CR-I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR-I (+). The first reported preparative SFC separations on Crownpak® CR-I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstr...
Source: Chirality - September 22, 2023 Category: Molecular Biology Authors: Lei Yue Larry Miller John Reilly Source Type: research
Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether-derived column
In this study, crown ether-derived column Crownpak® CR-I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide-based columns. Crownpak® CR-I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR-I (+). The first reported preparative SFC separations on Crownpak® CR-I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstr...
Source: Chirality - September 22, 2023 Category: Molecular Biology Authors: Lei Yue Larry Miller John Reilly Source Type: research
Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether-derived column
In this study, crown ether-derived column Crownpak® CR-I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide-based columns. Crownpak® CR-I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR-I (+). The first reported preparative SFC separations on Crownpak® CR-I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstr...
Source: Chirality - September 22, 2023 Category: Molecular Biology Authors: Lei Yue Larry Miller John Reilly Source Type: research
Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether-derived column
In this study, crown ether-derived column Crownpak® CR-I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide-based columns. Crownpak® CR-I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR-I (+). The first reported preparative SFC separations on Crownpak® CR-I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstr...
Source: Chirality - September 22, 2023 Category: Molecular Biology Authors: Lei Yue Larry Miller John Reilly Source Type: research
Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether-derived column
In this study, crown ether-derived column Crownpak® CR-I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide-based columns. Crownpak® CR-I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR-I (+). The first reported preparative SFC separations on Crownpak® CR-I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstr...
Source: Chirality - September 22, 2023 Category: Molecular Biology Authors: Lei Yue Larry Miller John Reilly Source Type: research
Green synthesis of (S)-1-(furan-2-yl)propan-1-ol from asymmetric bioreduction of 1-(furan-2-yl)propan-1-one using whole-cell of Lactobacillus paracasei BD101
In this study, the synthesis of (S)-2 was carried out by performing the asymmetric bioreduction of 1-(furan-2-yl)propan-1-one (1) using the Lactobacillus paracasei BD101 biocatalyst obtained from boza, a grain-based fermented beverage. (S)-2 was obtained with >99% conversion, >99% ee, and 96% yield under the optimized conditions. Furthermore, in 50 h, 8.37 g of 1 was entirely transformed into (S)-2 on gram scale (96% isolated yield, 8.11 g). This is the first report on the high-gram scale biocatalyzed synthesis of enantiopure (S)-2. These data suggest that L. paracasei BD101 can be used to bioreduction of 1 in gram s...
Source: Chirality - September 20, 2023 Category: Molecular Biology Authors: Ali Sava ş Bülbül Engin Şahin Source Type: research
Green synthesis of (S)-1-(furan-2-yl)propan-1-ol from asymmetric bioreduction of 1-(furan-2-yl)propan-1-one using whole-cell of Lactobacillus paracasei BD101
In this study, the synthesis of (S)-2 was carried out by performing the asymmetric bioreduction of 1-(furan-2-yl)propan-1-one (1) using the Lactobacillus paracasei BD101 biocatalyst obtained from boza, a grain-based fermented beverage. (S)-2 was obtained with >99% conversion, >99% ee, and 96% yield under the optimized conditions. Furthermore, in 50 h, 8.37 g of 1 was entirely transformed into (S)-2 on gram scale (96% isolated yield, 8.11 g). This is the first report on the high-gram scale biocatalyzed synthesis of enantiopure (S)-2. These data suggest that L. paracasei BD101 can be used to bioreduction of 1 in gram s...
Source: Chirality - September 20, 2023 Category: Molecular Biology Authors: Ali Sava ş Bülbül Engin Şahin Source Type: research
Enantiomerization and enantioselective bioaccumulation of diclofop-methyl in tubifex worms
In this study, the hydrolysis of diclofop-methyl (DM) in aqueous system and the bioaccumulation of its main metabolite, Diclofop (DA), in the tubifex worms were investigated using enantioselective high-performance liquid chromatography. With the addition of tubifex, rapid hydrolysis was observed for DM. It is revealed that the hydrolysis of DM in the water and the accumulation of DA in the worms were both enantioselective. Meanwhile, either the hydrolysis amount or the levels of enantioselectivity were influenced by the tubifex. After incubated for 24 h, about 94.6% of the DM was hydrolyzed and the enantiomer fraction of m...
Source: Chirality - September 18, 2023 Category: Molecular Biology Authors: Tiantian Liu Xinhong Duan Source Type: research
Enantiomerization and enantioselective bioaccumulation of diclofop-methyl in tubifex worms
In this study, the hydrolysis of diclofop-methyl (DM) in aqueous system and the bioaccumulation of its main metabolite, Diclofop (DA), in the tubifex worms were investigated using enantioselective high-performance liquid chromatography. With the addition of tubifex, rapid hydrolysis was observed for DM. It is revealed that the hydrolysis of DM in the water and the accumulation of DA in the worms were both enantioselective. Meanwhile, either the hydrolysis amount or the levels of enantioselectivity were influenced by the tubifex. After incubated for 24 h, about 94.6% of the DM was hydrolyzed and the enantiomer fraction of m...
Source: Chirality - September 18, 2023 Category: Molecular Biology Authors: Tiantian Liu Xinhong Duan Source Type: research
Enantiomerization and enantioselective bioaccumulation of diclofop-methyl in tubifex worms
In this study, the hydrolysis of diclofop-methyl (DM) in aqueous system and the bioaccumulation of its main metabolite, Diclofop (DA), in the tubifex worms were investigated using enantioselective high-performance liquid chromatography. With the addition of tubifex, rapid hydrolysis was observed for DM. It is revealed that the hydrolysis of DM in the water and the accumulation of DA in the worms were both enantioselective. Meanwhile, either the hydrolysis amount or the levels of enantioselectivity were influenced by the tubifex. After incubated for 24 h, about 94.6% of the DM was hydrolyzed and the enantiomer fraction of m...
Source: Chirality - September 18, 2023 Category: Molecular Biology Authors: Tiantian Liu Xinhong Duan Source Type: research
Chiral recognition mechanism studies of Tyr-Arg-Phe-Lys-NH < sub > 2 < /sub > tetrapeptide on crown ether-based chiral stationary phase
Chirality. 2023 Sep 12. doi: 10.1002/chir.23619. Online ahead of print.ABSTRACTEven though chiral recognition for crown-ether CSPs is generally understood, on a molecular level, exact mechanisms for the resolution are still unclear. Furthermore, short peptide analytes often contain multiple amino moieties capable of binding to the crown ether selector. In order to extend the understanding in chiral recognition mechanisms, polar organic mode separation of Tyr-Arg-Phe-Lys-NH2 tetrapeptide llll/dddd enantiomers on S- and R-(3,3'-diphenyl-1,1'-binaphthyl)-20-crown-6 stationary phases was studied with 50-mM perchloric acid in m...
Source: Chirality - September 13, 2023 Category: Molecular Biology Authors: Toms Upmanis Eduards Sevostjanovs Helena Ka žoka Source Type: research
Chiral recognition mechanism studies of Tyr-Arg-Phe-Lys-NH < sub > 2 < /sub > tetrapeptide on crown ether-based chiral stationary phase
Chirality. 2023 Sep 12. doi: 10.1002/chir.23619. Online ahead of print.ABSTRACTEven though chiral recognition for crown-ether CSPs is generally understood, on a molecular level, exact mechanisms for the resolution are still unclear. Furthermore, short peptide analytes often contain multiple amino moieties capable of binding to the crown ether selector. In order to extend the understanding in chiral recognition mechanisms, polar organic mode separation of Tyr-Arg-Phe-Lys-NH2 tetrapeptide llll/dddd enantiomers on S- and R-(3,3'-diphenyl-1,1'-binaphthyl)-20-crown-6 stationary phases was studied with 50-mM perchloric acid in m...
Source: Chirality - September 13, 2023 Category: Molecular Biology Authors: Toms Upmanis Eduards Sevostjanovs Helena Ka žoka Source Type: research
Chiral recognition mechanism studies of Tyr-Arg-Phe-Lys-NH < sub > 2 < /sub > tetrapeptide on crown ether-based chiral stationary phase
Chirality. 2023 Sep 12. doi: 10.1002/chir.23619. Online ahead of print.ABSTRACTEven though chiral recognition for crown-ether CSPs is generally understood, on a molecular level, exact mechanisms for the resolution are still unclear. Furthermore, short peptide analytes often contain multiple amino moieties capable of binding to the crown ether selector. In order to extend the understanding in chiral recognition mechanisms, polar organic mode separation of Tyr-Arg-Phe-Lys-NH2 tetrapeptide llll/dddd enantiomers on S- and R-(3,3'-diphenyl-1,1'-binaphthyl)-20-crown-6 stationary phases was studied with 50-mM perchloric acid in m...
Source: Chirality - September 13, 2023 Category: Molecular Biology Authors: Toms Upmanis Eduards Sevostjanovs Helena Ka žoka Source Type: research
