Stereoselective study of fluoxetine and norfluoxetine across the blood-brain barrier mediated by organic cation transporter 1/3 in rats using an enantioselective UPLC-MS/MS method
In this study, accurate and reliable UPLC-MS/MS enantioselective analysis was established in rat plasma and brain. The characteristics of FLT and NFLT enantiomers across the BBB were studied by chemical knockout of rat transporters. We found that the dominant enantiomers of FLT and NFLT were S-FLT and R-NFLT, respectively, both in plasma and in brain. The FLT and NFLT enantiomers showed significant stereoselectivity across the BBB, and S-FLT and S-NFLT were the dominant configurations across the BBB. Chemical knockout of organic cation transporter 1 (OCT1) and OCT3 can affect the ratio of plasma FLT and NFLT enantiomers in...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Hai An Jinjun Fang Min Wang Haiyang Lin Yingying Sun Bingying Hu Zhisheng He Zhen Ge Yang Wei Source Type: research
Chiral LC-MS/MS method for the discrimination of triiodothyronine enantiomers on a crown ether-based chiral stationary phase
Chirality. 2023 Jul 18. doi: 10.1002/chir.23611. Online ahead of print.ABSTRACTConsidering the greater pharmaceutical and clinical interest of triiodothyronine (T3 ) thyroid hormone, an effective D/L-T3 enantiomer separation was performed on a crown ether-based chiral stationary phase by LC-MS/MS. In optimal analytical condition and selected reaction monitoring mode, the two enantiomers of T3 were baseline separated within 10 min. The limit of detection and limit of quantitation were found to be 0.05 and 0.10 ng/μl; 0.20 and 0.50 ng/μl for D- and L-T3 , respectively. During validation, this method proved to be feasible, ...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Byeongchan Choi Suraj Adhikari Moong-Gi Ji Man-Jeong Paik Gwang Lee Wonjae Lee Source Type: research
Stereoselective study of fluoxetine and norfluoxetine across the blood-brain barrier mediated by organic cation transporter 1/3 in rats using an enantioselective UPLC-MS/MS method
In this study, accurate and reliable UPLC-MS/MS enantioselective analysis was established in rat plasma and brain. The characteristics of FLT and NFLT enantiomers across the BBB were studied by chemical knockout of rat transporters. We found that the dominant enantiomers of FLT and NFLT were S-FLT and R-NFLT, respectively, both in plasma and in brain. The FLT and NFLT enantiomers showed significant stereoselectivity across the BBB, and S-FLT and S-NFLT were the dominant configurations across the BBB. Chemical knockout of organic cation transporter 1 (OCT1) and OCT3 can affect the ratio of plasma FLT and NFLT enantiomers in...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Hai An Jinjun Fang Min Wang Haiyang Lin Yingying Sun Bingying Hu Zhisheng He Zhen Ge Yang Wei Source Type: research
Chiral LC-MS/MS method for the discrimination of triiodothyronine enantiomers on a crown ether-based chiral stationary phase
Chirality. 2023 Jul 18. doi: 10.1002/chir.23611. Online ahead of print.ABSTRACTConsidering the greater pharmaceutical and clinical interest of triiodothyronine (T3 ) thyroid hormone, an effective D/L-T3 enantiomer separation was performed on a crown ether-based chiral stationary phase by LC-MS/MS. In optimal analytical condition and selected reaction monitoring mode, the two enantiomers of T3 were baseline separated within 10 min. The limit of detection and limit of quantitation were found to be 0.05 and 0.10 ng/μl; 0.20 and 0.50 ng/μl for D- and L-T3 , respectively. During validation, this method proved to be feasible, ...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Byeongchan Choi Suraj Adhikari Moong-Gi Ji Man-Jeong Paik Gwang Lee Wonjae Lee Source Type: research
Stereoselective study of fluoxetine and norfluoxetine across the blood-brain barrier mediated by organic cation transporter 1/3 in rats using an enantioselective UPLC-MS/MS method
In this study, accurate and reliable UPLC-MS/MS enantioselective analysis was established in rat plasma and brain. The characteristics of FLT and NFLT enantiomers across the BBB were studied by chemical knockout of rat transporters. We found that the dominant enantiomers of FLT and NFLT were S-FLT and R-NFLT, respectively, both in plasma and in brain. The FLT and NFLT enantiomers showed significant stereoselectivity across the BBB, and S-FLT and S-NFLT were the dominant configurations across the BBB. Chemical knockout of organic cation transporter 1 (OCT1) and OCT3 can affect the ratio of plasma FLT and NFLT enantiomers in...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Hai An Jinjun Fang Min Wang Haiyang Lin Yingying Sun Bingying Hu Zhisheng He Zhen Ge Yang Wei Source Type: research
Chiral LC-MS/MS method for the discrimination of triiodothyronine enantiomers on a crown ether-based chiral stationary phase
Chirality. 2023 Jul 18. doi: 10.1002/chir.23611. Online ahead of print.ABSTRACTConsidering the greater pharmaceutical and clinical interest of triiodothyronine (T3 ) thyroid hormone, an effective D/L-T3 enantiomer separation was performed on a crown ether-based chiral stationary phase by LC-MS/MS. In optimal analytical condition and selected reaction monitoring mode, the two enantiomers of T3 were baseline separated within 10 min. The limit of detection and limit of quantitation were found to be 0.05 and 0.10 ng/μl; 0.20 and 0.50 ng/μl for D- and L-T3 , respectively. During validation, this method proved to be feasible, ...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Byeongchan Choi Suraj Adhikari Moong-Gi Ji Man-Jeong Paik Gwang Lee Wonjae Lee Source Type: research
Stereoselective study of fluoxetine and norfluoxetine across the blood-brain barrier mediated by organic cation transporter 1/3 in rats using an enantioselective UPLC-MS/MS method
In this study, accurate and reliable UPLC-MS/MS enantioselective analysis was established in rat plasma and brain. The characteristics of FLT and NFLT enantiomers across the BBB were studied by chemical knockout of rat transporters. We found that the dominant enantiomers of FLT and NFLT were S-FLT and R-NFLT, respectively, both in plasma and in brain. The FLT and NFLT enantiomers showed significant stereoselectivity across the BBB, and S-FLT and S-NFLT were the dominant configurations across the BBB. Chemical knockout of organic cation transporter 1 (OCT1) and OCT3 can affect the ratio of plasma FLT and NFLT enantiomers in...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Hai An Jinjun Fang Min Wang Haiyang Lin Yingying Sun Bingying Hu Zhisheng He Zhen Ge Yang Wei Source Type: research
Chiral LC-MS/MS method for the discrimination of triiodothyronine enantiomers on a crown ether-based chiral stationary phase
Chirality. 2023 Jul 18. doi: 10.1002/chir.23611. Online ahead of print.ABSTRACTConsidering the greater pharmaceutical and clinical interest of triiodothyronine (T3 ) thyroid hormone, an effective D/L-T3 enantiomer separation was performed on a crown ether-based chiral stationary phase by LC-MS/MS. In optimal analytical condition and selected reaction monitoring mode, the two enantiomers of T3 were baseline separated within 10 min. The limit of detection and limit of quantitation were found to be 0.05 and 0.10 ng/μl; 0.20 and 0.50 ng/μl for D- and L-T3 , respectively. During validation, this method proved to be feasible, ...
Source: Chirality - July 19, 2023 Category: Molecular Biology Authors: Byeongchan Choi Suraj Adhikari Moong-Gi Ji Man-Jeong Paik Gwang Lee Wonjae Lee Source Type: research
Separation and quantitative estimation of stereo-selective enantiomers of montelukast in pharmaceutical drug substance and tablets dosage forms by using stability-indicating normal phase-HPLC method
Chirality. 2023 Jul 17. doi: 10.1002/chir.23610. Online ahead of print.ABSTRACTMontelukast sodium (MLS) is a leukotriene receptor antagonist that relieves asthma, bronchospasm, allergic rhinitis, and urticaria. A simple, robust, and stability-indicating normal phase high-performance liquid chromatography method was developed to separate and quantitatively estimate the S-enantiomer of MLS. The chiral separation was achieved using USP L51 packing material along with a mobile phase consisting of a solvent mixture (n-hexane, ethanol, and propionic acid), a flow rate of 1.0 mL/min, a detection wavelength of 284 nm, a column tem...
Source: Chirality - July 18, 2023 Category: Molecular Biology Authors: Niroja Vadagam Sharath Babu Haridasyam Muvvala Venkatanarayana Narasimha S Lakka Sanjeeva R Chinnakadoori Source Type: research
Employment of chiral columns with superficially porous particles in chiral separations of cobalt bis (dicarbollide) and nido-7,8-C < sub > 2 < /sub > B < sub > 9 < /sub > H < sub > 12 < /sub > (1-) derivatives
Chirality. 2023 Jul 17. doi: 10.1002/chir.23606. Online ahead of print.ABSTRACTDerivatives of the nido-7,8-C2 B9 H12 (1-) (dicarbollide ion) and [3,3'-Co-(1,2-C2 B9 H11 )2 ](1-) cobalt sandwich (COSAN) ion represent groups of extremely chemically and thermally stable abiotic compounds. They are being investigated in many research areas, that is, medicinal chemistry, material sciences, analytical chemistry, and electrochemistry. The chirality of these compounds remains still grossly overlooked, what is also reflected in limited number of reports on their chiral separations. Continued progress depends on reliable, fast, and ...
Source: Chirality - July 18, 2023 Category: Molecular Biology Authors: Ond řej Horáček Umang Dhaubhadel Josef Holub Bohum ír Grüner Daniel W Armstrong Radim Ku čera Source Type: research
Separation and quantitative estimation of stereo-selective enantiomers of montelukast in pharmaceutical drug substance and tablets dosage forms by using stability-indicating normal phase-HPLC method
Chirality. 2023 Jul 17. doi: 10.1002/chir.23610. Online ahead of print.ABSTRACTMontelukast sodium (MLS) is a leukotriene receptor antagonist that relieves asthma, bronchospasm, allergic rhinitis, and urticaria. A simple, robust, and stability-indicating normal phase high-performance liquid chromatography method was developed to separate and quantitatively estimate the S-enantiomer of MLS. The chiral separation was achieved using USP L51 packing material along with a mobile phase consisting of a solvent mixture (n-hexane, ethanol, and propionic acid), a flow rate of 1.0 mL/min, a detection wavelength of 284 nm, a column tem...
Source: Chirality - July 18, 2023 Category: Molecular Biology Authors: Niroja Vadagam Sharath Babu Haridasyam Muvvala Venkatanarayana Narasimha S Lakka Sanjeeva R Chinnakadoori Source Type: research
Employment of chiral columns with superficially porous particles in chiral separations of cobalt bis (dicarbollide) and nido-7,8-C < sub > 2 < /sub > B < sub > 9 < /sub > H < sub > 12 < /sub > (1-) derivatives
Chirality. 2023 Jul 17. doi: 10.1002/chir.23606. Online ahead of print.ABSTRACTDerivatives of the nido-7,8-C2 B9 H12 (1-) (dicarbollide ion) and [3,3'-Co-(1,2-C2 B9 H11 )2 ](1-) cobalt sandwich (COSAN) ion represent groups of extremely chemically and thermally stable abiotic compounds. They are being investigated in many research areas, that is, medicinal chemistry, material sciences, analytical chemistry, and electrochemistry. The chirality of these compounds remains still grossly overlooked, what is also reflected in limited number of reports on their chiral separations. Continued progress depends on reliable, fast, and ...
Source: Chirality - July 18, 2023 Category: Molecular Biology Authors: Ond řej Horáček Umang Dhaubhadel Josef Holub Bohum ír Grüner Daniel W Armstrong Radim Ku čera Source Type: research
Triggering gel-sol transition by weak magnetic field
Chirality. 2023 Jul 15. doi: 10.1002/chir.23602. Online ahead of print.ABSTRACTThe self-assembly of small and always chiral molecules into fiber-like structures is a mysterious process, as the physics underlying such self-assembly is unclear. The energy necessary for this process exceeds the one provided by common dispersion interactions and hydrogen bonding. The recent results obtained by the scientific group of Prof. Naaman from the Weizmann Institute of Science fed light on the nature of forces providing for the self-assembly of chiral molecules and attributed these forces to spin-exchange interactions. Therefore, the s...
Source: Chirality - July 15, 2023 Category: Molecular Biology Authors: Sergey V Stovbun Anatoly M Zanin Aleksey A Skoblin Mariya G Mikhaleva Dmitry A Kuznetsov Dmitry V Zlenko Source Type: research
Triggering gel-sol transition by weak magnetic field
Chirality. 2023 Jul 15. doi: 10.1002/chir.23602. Online ahead of print.ABSTRACTThe self-assembly of small and always chiral molecules into fiber-like structures is a mysterious process, as the physics underlying such self-assembly is unclear. The energy necessary for this process exceeds the one provided by common dispersion interactions and hydrogen bonding. The recent results obtained by the scientific group of Prof. Naaman from the Weizmann Institute of Science fed light on the nature of forces providing for the self-assembly of chiral molecules and attributed these forces to spin-exchange interactions. Therefore, the s...
Source: Chirality - July 15, 2023 Category: Molecular Biology Authors: Sergey V Stovbun Anatoly M Zanin Aleksey A Skoblin Mariya G Mikhaleva Dmitry A Kuznetsov Dmitry V Zlenko Source Type: research
Triggering gel-sol transition by weak magnetic field
Chirality. 2023 Jul 15. doi: 10.1002/chir.23602. Online ahead of print.ABSTRACTThe self-assembly of small and always chiral molecules into fiber-like structures is a mysterious process, as the physics underlying such self-assembly is unclear. The energy necessary for this process exceeds the one provided by common dispersion interactions and hydrogen bonding. The recent results obtained by the scientific group of Prof. Naaman from the Weizmann Institute of Science fed light on the nature of forces providing for the self-assembly of chiral molecules and attributed these forces to spin-exchange interactions. Therefore, the s...
Source: Chirality - July 15, 2023 Category: Molecular Biology Authors: Sergey V Stovbun Anatoly M Zanin Aleksey A Skoblin Mariya G Mikhaleva Dmitry A Kuznetsov Dmitry V Zlenko Source Type: research
