A governance of ion selectivity based on the occupancy of the "beacon" in one- and four-domain calcium and sodium channels
Channels (Austin). 2023 Dec;17(1):2191773. doi: 10.1080/19336950.2023.2191773.ABSTRACTOne of nature's exceptions was discovered when a Cav3 T-type channel was observed to switch phenotype from a calcium channel into a sodium channel by neutralizing an aspartate residue in the high field strength (HFS) +1 position within the ion selectivity filter. The HFS+1 site is dubbed a "beacon" for its location at the entryway just above the constricted, minimum radius of the HFS site's electronegative ring. A classification is proposed based on the occupancy of the HFS+1 "beacon" which correlates with the calcium- or sodium-selectivi...
Source: Channels - April 21, 2023 Category: Molecular Biology Authors: J David Spafford Source Type: research
Effect of truncation on TRPM7 channel activity
Channels (Austin). 2023 Dec;17(1):2200874. doi: 10.1080/19336950.2023.2200874.ABSTRACTTransient receptor potential melastatin-like 7 (TRPM7) is a key player in various physiological and pathological processes. TRPM7 channel activity is regulated by different factors. The effects of cleavage of different domains on channel activity remain unknown. Here, we constructed several TRPM7 clones and explored the effects of truncating the mouse TRPM7 at different locations on the ion channel activity in two cell lines. We compared the clones' activity with the full-length TRPM7 and the native TRPM7 in transfected and untransfected ...
Source: Channels - April 11, 2023 Category: Molecular Biology Authors: Zhuqing Xie Nashat Abumaria Source Type: research
Fibrillin-1 mutation contributes to Marfan syndrome by inhibiting Cav1.2-mediated cell proliferation in vascular smooth muscle cells
Channels (Austin). 2023 Dec;17(1):2192377. doi: 10.1080/19336950.2023.2192377.ABSTRACTMarfan syndrome (MFS) is an autosomal dominant connective tissue disorder caused by mutation in fibrillin-1 (FBN1). However, the molecular mechanism underlying MFS remains poorly understood. The study aimed to explore how the L-type calcium channel (CaV1.2) modulates disease progression of MFS and to identify a potential effective target for attenuating MFS. KEGG enrichment analysis showed that the calcium signaling pathway gene set was significantly enriched. We demonstrated that FBN1 deficiency exhibited inhibition on both the expressio...
Source: Channels - March 27, 2023 Category: Molecular Biology Authors: Wenfeng Lin Jiaqi Xiong Yefan Jiang Hao Liu Jinhui Bian Juejin Wang Yongfeng Shao Buqing Ni Source Type: research
