Recent advances on the structure and the function relationships of the TRPV4 ion channel
Channels (Austin). 2024 Dec;18(1):2313323. doi: 10.1080/19336950.2024.2313323. Epub 2024 Feb 14.ABSTRACTThe members of the superfamily of Transient Receptor Potential (TRP) ion channels are physiologically important molecules that have been studied for many years and are still being intensively researched. Among the vanilloid TRP subfamily, the TRPV4 ion channel is an interesting protein due to its involvement in several essential physiological processes and in the development of various diseases. As in other proteins, changes in its function that lead to the development of pathological states, have been closely associated...
Source: Channels - February 14, 2024 Category: Molecular Biology Authors: Ra úl Sánchez-Hernández Miguel Ben ítez-Angeles Ana M Hern ández-Vega Tamara Rosenbaum Source Type: research
A phenylalanine at the extracellular side of Kir1.1 facilitates potassium permeation
Channels (Austin). 2024 Dec;18(1):2294661. doi: 10.1080/19336950.2023.2294661. Epub 2024 Jan 7.ABSTRACTThe Kir1.1 (ROMK) family of weak inward rectifiers controls K secretion in the renal CCT and K recycling in the renal TALH. A single point mutant of the inward rectifier, F127V-Kir1.1b was used to investigate the K transition between the selectivity filter and the outer mouth of the channel. We hypothesize that normally an aromatic Phe at the external entryway of Kir1.1b facilitates outward K secretion. We tested this by replacing F127-Kir1.1b with a small aliphatic Val. Results indicate that removal of the Phe at 127 sup...
Source: Channels - January 7, 2024 Category: Molecular Biology Authors: Henry Sackin Mikheil Nanazashvili Source Type: research
A phenylalanine at the extracellular side of Kir1.1 facilitates potassium permeation
Channels (Austin). 2024 Dec;18(1):2294661. doi: 10.1080/19336950.2023.2294661. Epub 2024 Jan 7.ABSTRACTThe Kir1.1 (ROMK) family of weak inward rectifiers controls K secretion in the renal CCT and K recycling in the renal TALH. A single point mutant of the inward rectifier, F127V-Kir1.1b was used to investigate the K transition between the selectivity filter and the outer mouth of the channel. We hypothesize that normally an aromatic Phe at the external entryway of Kir1.1b facilitates outward K secretion. We tested this by replacing F127-Kir1.1b with a small aliphatic Val. Results indicate that removal of the Phe at 127 sup...
Source: Channels - January 7, 2024 Category: Molecular Biology Authors: Henry Sackin Mikheil Nanazashvili Source Type: research
Novel insights into voltage-gated ion channels: Translational breakthroughs in medical oncology
Channels (Austin). 2024 Dec;18(1):2297605. doi: 10.1080/19336950.2023.2297605. Epub 2023 Dec 28.ABSTRACTPreclinical evidence suggests that voltage gradients can act as a kind of top-down master regulator during embryogenesis and orchestrate downstream molecular-genetic pathways during organ regeneration or repair. Moreover, electrical stimulation shifts response to injury toward regeneration instead of healing or scarring. Cancer and embryogenesis not only share common phenotypical features but also commonly upregulated molecular pathways. Voltage-gated ion channel activity is directly or indirectly linked to the pathogene...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Minas Sakellakis Sung Mi Yoon Jashan Reet Athanasios Chalkias Source Type: research
Reducing agents facilitate membrane patch seal integrity and longevity
In this report, we describe our observation that the application of reducing agents (DTT and TCEP) to the external bath solution during whole-cell patch clamp recordings of heterologous cells (HEK and LM) and cultured primary cells (DRG neurons) enhanced the success of giga-ohm seal formation. Reducing agents also maintained the integrity of the seal for longer periods of time at strong hyperpolarizing voltages, whereas an oxidizing agent (H2O2) appeared to have the opposite effect. In summary, we report a useful tool to improve the quality of patch clamp recordings that may be helpful in certain experimental contexts.PMID...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Damayantee Das Anson Wong Timothy N Friedman Bradley J Kerr Harley T Kurata Shawn M Lamothe Source Type: research
Novel insights into voltage-gated ion channels: Translational breakthroughs in medical oncology
Channels (Austin). 2024 Dec;18(1):2297605. doi: 10.1080/19336950.2023.2297605. Epub 2023 Dec 28.ABSTRACTPreclinical evidence suggests that voltage gradients can act as a kind of top-down master regulator during embryogenesis and orchestrate downstream molecular-genetic pathways during organ regeneration or repair. Moreover, electrical stimulation shifts response to injury toward regeneration instead of healing or scarring. Cancer and embryogenesis not only share common phenotypical features but also commonly upregulated molecular pathways. Voltage-gated ion channel activity is directly or indirectly linked to the pathogene...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Minas Sakellakis Sung Mi Yoon Jashan Reet Athanasios Chalkias Source Type: research
Reducing agents facilitate membrane patch seal integrity and longevity
In this report, we describe our observation that the application of reducing agents (DTT and TCEP) to the external bath solution during whole-cell patch clamp recordings of heterologous cells (HEK and LM) and cultured primary cells (DRG neurons) enhanced the success of giga-ohm seal formation. Reducing agents also maintained the integrity of the seal for longer periods of time at strong hyperpolarizing voltages, whereas an oxidizing agent (H2O2) appeared to have the opposite effect. In summary, we report a useful tool to improve the quality of patch clamp recordings that may be helpful in certain experimental contexts.PMID...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Damayantee Das Anson Wong Timothy N Friedman Bradley J Kerr Harley T Kurata Shawn M Lamothe Source Type: research
Novel insights into voltage-gated ion channels: Translational breakthroughs in medical oncology
Channels (Austin). 2024 Dec;18(1):2297605. doi: 10.1080/19336950.2023.2297605. Epub 2023 Dec 28.ABSTRACTPreclinical evidence suggests that voltage gradients can act as a kind of top-down master regulator during embryogenesis and orchestrate downstream molecular-genetic pathways during organ regeneration or repair. Moreover, electrical stimulation shifts response to injury toward regeneration instead of healing or scarring. Cancer and embryogenesis not only share common phenotypical features but also commonly upregulated molecular pathways. Voltage-gated ion channel activity is directly or indirectly linked to the pathogene...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Minas Sakellakis Sung Mi Yoon Jashan Reet Athanasios Chalkias Source Type: research
Reducing agents facilitate membrane patch seal integrity and longevity
In this report, we describe our observation that the application of reducing agents (DTT and TCEP) to the external bath solution during whole-cell patch clamp recordings of heterologous cells (HEK and LM) and cultured primary cells (DRG neurons) enhanced the success of giga-ohm seal formation. Reducing agents also maintained the integrity of the seal for longer periods of time at strong hyperpolarizing voltages, whereas an oxidizing agent (H2O2) appeared to have the opposite effect. In summary, we report a useful tool to improve the quality of patch clamp recordings that may be helpful in certain experimental contexts.PMID...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Damayantee Das Anson Wong Timothy N Friedman Bradley J Kerr Harley T Kurata Shawn M Lamothe Source Type: research
Novel insights into voltage-gated ion channels: Translational breakthroughs in medical oncology
Channels (Austin). 2024 Dec;18(1):2297605. doi: 10.1080/19336950.2023.2297605. Epub 2023 Dec 28.ABSTRACTPreclinical evidence suggests that voltage gradients can act as a kind of top-down master regulator during embryogenesis and orchestrate downstream molecular-genetic pathways during organ regeneration or repair. Moreover, electrical stimulation shifts response to injury toward regeneration instead of healing or scarring. Cancer and embryogenesis not only share common phenotypical features but also commonly upregulated molecular pathways. Voltage-gated ion channel activity is directly or indirectly linked to the pathogene...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Minas Sakellakis Sung Mi Yoon Jashan Reet Athanasios Chalkias Source Type: research
Reducing agents facilitate membrane patch seal integrity and longevity
In this report, we describe our observation that the application of reducing agents (DTT and TCEP) to the external bath solution during whole-cell patch clamp recordings of heterologous cells (HEK and LM) and cultured primary cells (DRG neurons) enhanced the success of giga-ohm seal formation. Reducing agents also maintained the integrity of the seal for longer periods of time at strong hyperpolarizing voltages, whereas an oxidizing agent (H2O2) appeared to have the opposite effect. In summary, we report a useful tool to improve the quality of patch clamp recordings that may be helpful in certain experimental contexts.PMID...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Damayantee Das Anson Wong Timothy N Friedman Bradley J Kerr Harley T Kurata Shawn M Lamothe Source Type: research
