Secretory carrier-associated membrane protein 5 regulates cell-surface targeting of T-type calcium channels
Channels (Austin). 2023 Dec;17(1):2230776. doi: 10.1080/19336950.2023.2230776.ABSTRACTMissense mutations in the human secretary carrier-associated membrane protein 5 (SCAMP5) cause a variety of neurological disorders including neurodevelopmental delay, epilepsy, and Parkinson's disease. We recently documented the importance of SCAMP2 in the regulation of T-type calcium channel expression in the plasma membrane. Here, we show that similar to SCAMP2, the co-expression of SCAMP5 in tsA-201 cells expressing recombinant Cav3.1, Cav3.2, and Cav3.3 channels nearly abolished whole-cell T-type currents. Recording of intramembrane c...
Source: Channels - June 30, 2023 Category: Molecular Biology Authors: Emilio R Mustaf á Konstantin Wei ß Norbert Weiss Source Type: research
Cycling matters: Sex hormone regulation of vascular potassium channels
Channels (Austin). 2023 Dec;17(1):2217637. doi: 10.1080/19336950.2023.2217637.ABSTRACTSex hormones and the reproductive cycle (estrus in rodents and menstrual in humans) have a known impact on arterial function. In spite of this, sex hormones and the estrus/menstrual cycle are often neglected experimental factors in vascular basic preclinical scientific research. Recent research by our own laboratory indicates that cyclical changes in serum concentrations of sex -hormones across the rat estrus cycle, primary estradiol, have significant consequences for the subcellular trafficking and function of KV. Vascular potassium chan...
Source: Channels - May 27, 2023 Category: Molecular Biology Authors: Samuel N Baldwin Thomas A Jepps Iain A Greenwood Source Type: research
