Novel insights into voltage-gated ion channels: Translational breakthroughs in medical oncology
Channels (Austin). 2024 Dec;18(1):2297605. doi: 10.1080/19336950.2023.2297605. Epub 2023 Dec 28.ABSTRACTPreclinical evidence suggests that voltage gradients can act as a kind of top-down master regulator during embryogenesis and orchestrate downstream molecular-genetic pathways during organ regeneration or repair. Moreover, electrical stimulation shifts response to injury toward regeneration instead of healing or scarring. Cancer and embryogenesis not only share common phenotypical features but also commonly upregulated molecular pathways. Voltage-gated ion channel activity is directly or indirectly linked to the pathogene...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Minas Sakellakis Sung Mi Yoon Jashan Reet Athanasios Chalkias Source Type: research
Reducing agents facilitate membrane patch seal integrity and longevity
In this report, we describe our observation that the application of reducing agents (DTT and TCEP) to the external bath solution during whole-cell patch clamp recordings of heterologous cells (HEK and LM) and cultured primary cells (DRG neurons) enhanced the success of giga-ohm seal formation. Reducing agents also maintained the integrity of the seal for longer periods of time at strong hyperpolarizing voltages, whereas an oxidizing agent (H2O2) appeared to have the opposite effect. In summary, we report a useful tool to improve the quality of patch clamp recordings that may be helpful in certain experimental contexts.PMID...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Damayantee Das Anson Wong Timothy N Friedman Bradley J Kerr Harley T Kurata Shawn M Lamothe Source Type: research
Novel insights into voltage-gated ion channels: Translational breakthroughs in medical oncology
Channels (Austin). 2024 Dec;18(1):2297605. doi: 10.1080/19336950.2023.2297605. Epub 2023 Dec 28.ABSTRACTPreclinical evidence suggests that voltage gradients can act as a kind of top-down master regulator during embryogenesis and orchestrate downstream molecular-genetic pathways during organ regeneration or repair. Moreover, electrical stimulation shifts response to injury toward regeneration instead of healing or scarring. Cancer and embryogenesis not only share common phenotypical features but also commonly upregulated molecular pathways. Voltage-gated ion channel activity is directly or indirectly linked to the pathogene...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Minas Sakellakis Sung Mi Yoon Jashan Reet Athanasios Chalkias Source Type: research
Reducing agents facilitate membrane patch seal integrity and longevity
In this report, we describe our observation that the application of reducing agents (DTT and TCEP) to the external bath solution during whole-cell patch clamp recordings of heterologous cells (HEK and LM) and cultured primary cells (DRG neurons) enhanced the success of giga-ohm seal formation. Reducing agents also maintained the integrity of the seal for longer periods of time at strong hyperpolarizing voltages, whereas an oxidizing agent (H2O2) appeared to have the opposite effect. In summary, we report a useful tool to improve the quality of patch clamp recordings that may be helpful in certain experimental contexts.PMID...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Damayantee Das Anson Wong Timothy N Friedman Bradley J Kerr Harley T Kurata Shawn M Lamothe Source Type: research
Novel insights into voltage-gated ion channels: Translational breakthroughs in medical oncology
Channels (Austin). 2024 Dec;18(1):2297605. doi: 10.1080/19336950.2023.2297605. Epub 2023 Dec 28.ABSTRACTPreclinical evidence suggests that voltage gradients can act as a kind of top-down master regulator during embryogenesis and orchestrate downstream molecular-genetic pathways during organ regeneration or repair. Moreover, electrical stimulation shifts response to injury toward regeneration instead of healing or scarring. Cancer and embryogenesis not only share common phenotypical features but also commonly upregulated molecular pathways. Voltage-gated ion channel activity is directly or indirectly linked to the pathogene...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Minas Sakellakis Sung Mi Yoon Jashan Reet Athanasios Chalkias Source Type: research
Reducing agents facilitate membrane patch seal integrity and longevity
In this report, we describe our observation that the application of reducing agents (DTT and TCEP) to the external bath solution during whole-cell patch clamp recordings of heterologous cells (HEK and LM) and cultured primary cells (DRG neurons) enhanced the success of giga-ohm seal formation. Reducing agents also maintained the integrity of the seal for longer periods of time at strong hyperpolarizing voltages, whereas an oxidizing agent (H2O2) appeared to have the opposite effect. In summary, we report a useful tool to improve the quality of patch clamp recordings that may be helpful in certain experimental contexts.PMID...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Damayantee Das Anson Wong Timothy N Friedman Bradley J Kerr Harley T Kurata Shawn M Lamothe Source Type: research
Novel insights into voltage-gated ion channels: Translational breakthroughs in medical oncology
Channels (Austin). 2024 Dec;18(1):2297605. doi: 10.1080/19336950.2023.2297605. Epub 2023 Dec 28.ABSTRACTPreclinical evidence suggests that voltage gradients can act as a kind of top-down master regulator during embryogenesis and orchestrate downstream molecular-genetic pathways during organ regeneration or repair. Moreover, electrical stimulation shifts response to injury toward regeneration instead of healing or scarring. Cancer and embryogenesis not only share common phenotypical features but also commonly upregulated molecular pathways. Voltage-gated ion channel activity is directly or indirectly linked to the pathogene...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Minas Sakellakis Sung Mi Yoon Jashan Reet Athanasios Chalkias Source Type: research
Reducing agents facilitate membrane patch seal integrity and longevity
In this report, we describe our observation that the application of reducing agents (DTT and TCEP) to the external bath solution during whole-cell patch clamp recordings of heterologous cells (HEK and LM) and cultured primary cells (DRG neurons) enhanced the success of giga-ohm seal formation. Reducing agents also maintained the integrity of the seal for longer periods of time at strong hyperpolarizing voltages, whereas an oxidizing agent (H2O2) appeared to have the opposite effect. In summary, we report a useful tool to improve the quality of patch clamp recordings that may be helpful in certain experimental contexts.PMID...
Source: Channels - December 28, 2023 Category: Molecular Biology Authors: Damayantee Das Anson Wong Timothy N Friedman Bradley J Kerr Harley T Kurata Shawn M Lamothe Source Type: research
Structural biology of voltage-gated calcium channels
Channels (Austin). 2024 Dec;18(1):2290807. doi: 10.1080/19336950.2023.2290807. Epub 2023 Dec 7.ABSTRACTVoltage-gated calcium (Cav) channels mediate Ca2+ influx in response to membrane depolarization, playing critical roles in diverse physiological processes. Dysfunction or aberrant regulation of Cav channels can lead to life-threatening consequences. Cav-targeting drugs have been clinically used to treat cardiovascular and neuronal disorders for several decades. This review aims to provide an account of recent developments in the structural dissection of Cav channels. High-resolution structures have significantly advanced ...
Source: Channels - December 8, 2023 Category: Molecular Biology Authors: Xia Yao Shuai Gao Nieng Yan Source Type: research
