Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Chronic IFN α treatment induces leukopoiesis, increased plasma succinate and immune cell metabolic rewiring
Cell Immunol. 2023 Jun 20;390:104741. doi: 10.1016/j.cellimm.2023.104741. Online ahead of print.ABSTRACTAlthough clinically effective, the actions of IFNα, either produced endogenously or by therapeutic delivery, remain poorly understood. Emblematic of this research gap is the disparate array of notable side effects that occur in susceptible individuals, such as neuropsychiatric consequences, autoimmune phenomena, and infectious complications. We hypothesised that these complications are driven at least in part by dysregulated cellular metabolism. Male Wistar rats were treated with either 170,000 IU/kg human recombinant I...
Source: Cellular Immunology - June 25, 2023 Category: Allergy & Immunology Authors: Anjali S Yennemadi Gr áinne Jameson Mary Glass Carolina De Pasquale Joseph Keane Massimiliano Bianchi Gina Leisching Source Type: research
Interleukin-17 receptor C is essential for the pro-inflammatory pathogenicity of granulocyte-macrophage-colony-stimulating factor-producing T helper cells in experimental autoimmune uveitis
This study aims to understand the role of granulocyte-macrophage-colony-stimulating factor (GM-CSF)-producing T helper (ThGM) cells in the pathophysiology of mouse experimental autoimmune uveitis (EAU). We established an EAU model by immunizing mice with interphotoreceptor retinoid-binding protein (IRBP) 651-670. Splenic or eye-infiltrating ThGM cells were analyzed and enriched by flow cytometry according to the levels of an array of surface markers, transcription factors, and cytokines. Lentiviral transduction was conducted to silence or overexpress the target gene in differentiated ThGM cells. The adoptive transfer was a...
Source: Cellular Immunology - June 19, 2023 Category: Allergy & Immunology Authors: Lina Wu Lu Wang Xin Chai Source Type: research
Interleukin-17 receptor C is essential for the pro-inflammatory pathogenicity of granulocyte-macrophage-colony-stimulating factor-producing T helper cells in experimental autoimmune uveitis
This study aims to understand the role of granulocyte-macrophage-colony-stimulating factor (GM-CSF)-producing T helper (ThGM) cells in the pathophysiology of mouse experimental autoimmune uveitis (EAU). We established an EAU model by immunizing mice with interphotoreceptor retinoid-binding protein (IRBP) 651-670. Splenic or eye-infiltrating ThGM cells were analyzed and enriched by flow cytometry according to the levels of an array of surface markers, transcription factors, and cytokines. Lentiviral transduction was conducted to silence or overexpress the target gene in differentiated ThGM cells. The adoptive transfer was a...
Source: Cellular Immunology - June 19, 2023 Category: Allergy & Immunology Authors: Lina Wu Lu Wang Xin Chai Source Type: research
Interleukin-17 receptor C is essential for the pro-inflammatory pathogenicity of granulocyte-macrophage-colony-stimulating factor-producing T helper cells in experimental autoimmune uveitis
This study aims to understand the role of granulocyte-macrophage-colony-stimulating factor (GM-CSF)-producing T helper (ThGM) cells in the pathophysiology of mouse experimental autoimmune uveitis (EAU). We established an EAU model by immunizing mice with interphotoreceptor retinoid-binding protein (IRBP) 651-670. Splenic or eye-infiltrating ThGM cells were analyzed and enriched by flow cytometry according to the levels of an array of surface markers, transcription factors, and cytokines. Lentiviral transduction was conducted to silence or overexpress the target gene in differentiated ThGM cells. The adoptive transfer was a...
Source: Cellular Immunology - June 19, 2023 Category: Allergy & Immunology Authors: Lina Wu Lu Wang Xin Chai Source Type: research
Interleukin-17 receptor C is essential for the pro-inflammatory pathogenicity of granulocyte-macrophage-colony-stimulating factor-producing T helper cells in experimental autoimmune uveitis
This study aims to understand the role of granulocyte-macrophage-colony-stimulating factor (GM-CSF)-producing T helper (ThGM) cells in the pathophysiology of mouse experimental autoimmune uveitis (EAU). We established an EAU model by immunizing mice with interphotoreceptor retinoid-binding protein (IRBP) 651-670. Splenic or eye-infiltrating ThGM cells were analyzed and enriched by flow cytometry according to the levels of an array of surface markers, transcription factors, and cytokines. Lentiviral transduction was conducted to silence or overexpress the target gene in differentiated ThGM cells. The adoptive transfer was a...
Source: Cellular Immunology - June 19, 2023 Category: Allergy & Immunology Authors: Lina Wu Lu Wang Xin Chai Source Type: research
Interleukin-17 receptor C is essential for the pro-inflammatory pathogenicity of granulocyte-macrophage-colony-stimulating factor-producing T helper cells in experimental autoimmune uveitis
This study aims to understand the role of granulocyte-macrophage-colony-stimulating factor (GM-CSF)-producing T helper (ThGM) cells in the pathophysiology of mouse experimental autoimmune uveitis (EAU). We established an EAU model by immunizing mice with interphotoreceptor retinoid-binding protein (IRBP) 651-670. Splenic or eye-infiltrating ThGM cells were analyzed and enriched by flow cytometry according to the levels of an array of surface markers, transcription factors, and cytokines. Lentiviral transduction was conducted to silence or overexpress the target gene in differentiated ThGM cells. The adoptive transfer was a...
Source: Cellular Immunology - June 19, 2023 Category: Allergy & Immunology Authors: Lina Wu Lu Wang Xin Chai Source Type: research
