SARS-CoV2 Nsp1 is a metal-dependent DNA and RNA endonuclease
Biometals. 2024 Mar 28. doi: 10.1007/s10534-024-00596-z. Online ahead of print.ABSTRACTOver recent years, we have been living under a pandemic, caused by the rapid spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV2). One of the major virulence factors of Coronaviruses is the Non-structural protein 1 (Nsp1), known to suppress the host cells protein translation machinery, allowing the virus to produce its own proteins, propagate and invade new cells. To unveil the molecular mechanisms of SARS-CoV2 Nsp1, we have addressed its biochemical and biophysical properties in the presence of calcium, magnesium an...
Source: Biometals - March 28, 2024 Category: Biochemistry Authors: Bruno A Salgueiro Margarida Saramago Mark D Tully Federico Issoglio Sara T N Silva Ana C F Paiva Cec ília M Arraiano Pedro M Matias Rute G Matos Elin Moe C élia V Romão Source Type: research
Insight into the role of copper-based materials against the coronaviruses MHV-3, a model for SARS-CoV-2, during the COVID-19 pandemic
In this study, we evaluated the cytotoxicity and virucidal effectiveness of products and materials containing copper against mouse hepatitis virus (MHV-3), a surrogate model for SARS-CoV-2. The results demonstrate that pure CuO and Cu possess activity against the enveloped virus at very low concentrations, ranging from 0.001 to 0.1% (w/v). A greater virucidal efficacy of CuO was found for nanoparticles, which showed activity even against viruses that are more resistant to disinfection such as feline calicivirus (FCV). Most of the evaluated products, with concentrations of Cu or CuO between 0.003 and 15% (w/v), were effecti...
Source: Biometals - March 19, 2024 Category: Biochemistry Authors: Gislaine S Jacinto Leonardo F G Dias Junko Tsukamoto Paulo N Lisboa-Filho Marina T Souza Ana Paula de Moraes Clarice W Arns Source Type: research
Insight into the role of copper-based materials against the coronaviruses MHV-3, a model for SARS-CoV-2, during the COVID-19 pandemic
In this study, we evaluated the cytotoxicity and virucidal effectiveness of products and materials containing copper against mouse hepatitis virus (MHV-3), a surrogate model for SARS-CoV-2. The results demonstrate that pure CuO and Cu possess activity against the enveloped virus at very low concentrations, ranging from 0.001 to 0.1% (w/v). A greater virucidal efficacy of CuO was found for nanoparticles, which showed activity even against viruses that are more resistant to disinfection such as feline calicivirus (FCV). Most of the evaluated products, with concentrations of Cu or CuO between 0.003 and 15% (w/v), were effecti...
Source: Biometals - March 19, 2024 Category: Biochemistry Authors: Gislaine S Jacinto Leonardo F G Dias Junko Tsukamoto Paulo N Lisboa-Filho Marina T Souza Ana Paula de Moraes Clarice W Arns Source Type: research
Insight into the role of copper-based materials against the coronaviruses MHV-3, a model for SARS-CoV-2, during the COVID-19 pandemic
In this study, we evaluated the cytotoxicity and virucidal effectiveness of products and materials containing copper against mouse hepatitis virus (MHV-3), a surrogate model for SARS-CoV-2. The results demonstrate that pure CuO and Cu possess activity against the enveloped virus at very low concentrations, ranging from 0.001 to 0.1% (w/v). A greater virucidal efficacy of CuO was found for nanoparticles, which showed activity even against viruses that are more resistant to disinfection such as feline calicivirus (FCV). Most of the evaluated products, with concentrations of Cu or CuO between 0.003 and 15% (w/v), were effecti...
Source: Biometals - March 19, 2024 Category: Biochemistry Authors: Gislaine S Jacinto Leonardo F G Dias Junko Tsukamoto Paulo N Lisboa-Filho Marina T Souza Ana Paula de Moraes Clarice W Arns Source Type: research
Insight into the role of copper-based materials against the coronaviruses MHV-3, a model for SARS-CoV-2, during the COVID-19 pandemic
In this study, we evaluated the cytotoxicity and virucidal effectiveness of products and materials containing copper against mouse hepatitis virus (MHV-3), a surrogate model for SARS-CoV-2. The results demonstrate that pure CuO and Cu possess activity against the enveloped virus at very low concentrations, ranging from 0.001 to 0.1% (w/v). A greater virucidal efficacy of CuO was found for nanoparticles, which showed activity even against viruses that are more resistant to disinfection such as feline calicivirus (FCV). Most of the evaluated products, with concentrations of Cu or CuO between 0.003 and 15% (w/v), were effecti...
Source: Biometals - March 19, 2024 Category: Biochemistry Authors: Gislaine S Jacinto Leonardo F G Dias Junko Tsukamoto Paulo N Lisboa-Filho Marina T Souza Ana Paula de Moraes Clarice W Arns Source Type: research
Insight into the role of copper-based materials against the coronaviruses MHV-3, a model for SARS-CoV-2, during the COVID-19 pandemic
In this study, we evaluated the cytotoxicity and virucidal effectiveness of products and materials containing copper against mouse hepatitis virus (MHV-3), a surrogate model for SARS-CoV-2. The results demonstrate that pure CuO and Cu possess activity against the enveloped virus at very low concentrations, ranging from 0.001 to 0.1% (w/v). A greater virucidal efficacy of CuO was found for nanoparticles, which showed activity even against viruses that are more resistant to disinfection such as feline calicivirus (FCV). Most of the evaluated products, with concentrations of Cu or CuO between 0.003 and 15% (w/v), were effecti...
Source: Biometals - March 19, 2024 Category: Biochemistry Authors: Gislaine S Jacinto Leonardo F G Dias Junko Tsukamoto Paulo N Lisboa-Filho Marina T Souza Ana Paula de Moraes Clarice W Arns Source Type: research
Insight into the role of copper-based materials against the coronaviruses MHV-3, a model for SARS-CoV-2, during the COVID-19 pandemic
In this study, we evaluated the cytotoxicity and virucidal effectiveness of products and materials containing copper against mouse hepatitis virus (MHV-3), a surrogate model for SARS-CoV-2. The results demonstrate that pure CuO and Cu possess activity against the enveloped virus at very low concentrations, ranging from 0.001 to 0.1% (w/v). A greater virucidal efficacy of CuO was found for nanoparticles, which showed activity even against viruses that are more resistant to disinfection such as feline calicivirus (FCV). Most of the evaluated products, with concentrations of Cu or CuO between 0.003 and 15% (w/v), were effecti...
Source: Biometals - March 19, 2024 Category: Biochemistry Authors: Gislaine S Jacinto Leonardo F G Dias Junko Tsukamoto Paulo N Lisboa-Filho Marina T Souza Ana Paula de Moraes Clarice W Arns Source Type: research
Insight into the role of copper-based materials against the coronaviruses MHV-3, a model for SARS-CoV-2, during the COVID-19 pandemic
In this study, we evaluated the cytotoxicity and virucidal effectiveness of products and materials containing copper against mouse hepatitis virus (MHV-3), a surrogate model for SARS-CoV-2. The results demonstrate that pure CuO and Cu possess activity against the enveloped virus at very low concentrations, ranging from 0.001 to 0.1% (w/v). A greater virucidal efficacy of CuO was found for nanoparticles, which showed activity even against viruses that are more resistant to disinfection such as feline calicivirus (FCV). Most of the evaluated products, with concentrations of Cu or CuO between 0.003 and 15% (w/v), were effecti...
Source: Biometals - March 19, 2024 Category: Biochemistry Authors: Gislaine S Jacinto Leonardo F G Dias Junko Tsukamoto Paulo N Lisboa-Filho Marina T Souza Ana Paula de Moraes Clarice W Arns Source Type: research
Investigation of the mutual crosstalk between ER stress and PI3K/AKT/mTOR signaling pathway in iron overload-induced liver injury in chicks
This study aims to investigate the interaction between the endoplasmic reticulum stress signaling pathway and the PI3K/AKT/mTOR signaling pathway in liver injury induced by iron overload in chicks. Rspectively, 150 one-day-old broilers were divided into three groups and supplemented with 50 (C), 500 (E1), and 1000 (E2) mg ferrous sulfate monohydrate/kg in the basal diet. Samples were taken after continuous feeding for 14 days. The results showed that iron overload could upregulate the levels of ALT and AST. Histopathological examination revealed bleeding in the central vein of the liver accompanied by inflammatory cell inf...
Source: Biometals - March 14, 2024 Category: Biochemistry Authors: Xiang-Long Lv Wen-Lei Li Feng-Jiao Sun Yu-Zhi An Ning Sun Xiao-Ping Lv Xue-Li Gao Source Type: research
Investigation of the mutual crosstalk between ER stress and PI3K/AKT/mTOR signaling pathway in iron overload-induced liver injury in chicks
This study aims to investigate the interaction between the endoplasmic reticulum stress signaling pathway and the PI3K/AKT/mTOR signaling pathway in liver injury induced by iron overload in chicks. Rspectively, 150 one-day-old broilers were divided into three groups and supplemented with 50 (C), 500 (E1), and 1000 (E2) mg ferrous sulfate monohydrate/kg in the basal diet. Samples were taken after continuous feeding for 14 days. The results showed that iron overload could upregulate the levels of ALT and AST. Histopathological examination revealed bleeding in the central vein of the liver accompanied by inflammatory cell inf...
Source: Biometals - March 14, 2024 Category: Biochemistry Authors: Xiang-Long Lv Wen-Lei Li Feng-Jiao Sun Yu-Zhi An Ning Sun Xiao-Ping Lv Xue-Li Gao Source Type: research
Investigation of the mutual crosstalk between ER stress and PI3K/AKT/mTOR signaling pathway in iron overload-induced liver injury in chicks
This study aims to investigate the interaction between the endoplasmic reticulum stress signaling pathway and the PI3K/AKT/mTOR signaling pathway in liver injury induced by iron overload in chicks. Rspectively, 150 one-day-old broilers were divided into three groups and supplemented with 50 (C), 500 (E1), and 1000 (E2) mg ferrous sulfate monohydrate/kg in the basal diet. Samples were taken after continuous feeding for 14 days. The results showed that iron overload could upregulate the levels of ALT and AST. Histopathological examination revealed bleeding in the central vein of the liver accompanied by inflammatory cell inf...
Source: Biometals - March 14, 2024 Category: Biochemistry Authors: Xiang-Long Lv Wen-Lei Li Feng-Jiao Sun Yu-Zhi An Ning Sun Xiao-Ping Lv Xue-Li Gao Source Type: research
Investigation of the mutual crosstalk between ER stress and PI3K/AKT/mTOR signaling pathway in iron overload-induced liver injury in chicks
This study aims to investigate the interaction between the endoplasmic reticulum stress signaling pathway and the PI3K/AKT/mTOR signaling pathway in liver injury induced by iron overload in chicks. Rspectively, 150 one-day-old broilers were divided into three groups and supplemented with 50 (C), 500 (E1), and 1000 (E2) mg ferrous sulfate monohydrate/kg in the basal diet. Samples were taken after continuous feeding for 14 days. The results showed that iron overload could upregulate the levels of ALT and AST. Histopathological examination revealed bleeding in the central vein of the liver accompanied by inflammatory cell inf...
Source: Biometals - March 14, 2024 Category: Biochemistry Authors: Xiang-Long Lv Wen-Lei Li Feng-Jiao Sun Yu-Zhi An Ning Sun Xiao-Ping Lv Xue-Li Gao Source Type: research
Metals and metallothionein evolution in snails: a contribution to the concept of metal-specific functionality from an animal model group
Biometals. 2024 Feb 28. doi: 10.1007/s10534-024-00584-3. Online ahead of print.ABSTRACTThis is a critical review of what we know so far about the evolution of metallothioneins (MTs) in Gastropoda (snails, whelks, limpets and slugs), an important class of molluscs with over 90,000 known species. Particular attention will be paid to the evolution of snail MTs in relation to the role of some metallic trace elements (cadmium, zinc and copper) and their interaction with MTs, also compared to MTs from other animal phyla. The article also highlights the important distinction, yet close relationship, between the structural and met...
Source: Biometals - February 28, 2024 Category: Biochemistry Authors: Reinhard Dallinger Source Type: research
Metals and metallothionein evolution in snails: a contribution to the concept of metal-specific functionality from an animal model group
Biometals. 2024 Feb 28. doi: 10.1007/s10534-024-00584-3. Online ahead of print.ABSTRACTThis is a critical review of what we know so far about the evolution of metallothioneins (MTs) in Gastropoda (snails, whelks, limpets and slugs), an important class of molluscs with over 90,000 known species. Particular attention will be paid to the evolution of snail MTs in relation to the role of some metallic trace elements (cadmium, zinc and copper) and their interaction with MTs, also compared to MTs from other animal phyla. The article also highlights the important distinction, yet close relationship, between the structural and met...
Source: Biometals - February 28, 2024 Category: Biochemistry Authors: Reinhard Dallinger Source Type: research
Metals and metallothionein evolution in snails: a contribution to the concept of metal-specific functionality from an animal model group
Biometals. 2024 Feb 28. doi: 10.1007/s10534-024-00584-3. Online ahead of print.ABSTRACTThis is a critical review of what we know so far about the evolution of metallothioneins (MTs) in Gastropoda (snails, whelks, limpets and slugs), an important class of molluscs with over 90,000 known species. Particular attention will be paid to the evolution of snail MTs in relation to the role of some metallic trace elements (cadmium, zinc and copper) and their interaction with MTs, also compared to MTs from other animal phyla. The article also highlights the important distinction, yet close relationship, between the structural and met...
Source: Biometals - February 28, 2024 Category: Biochemistry Authors: Reinhard Dallinger Source Type: research
