Schwann cells in neuromuscular < em > in vitro < /em > models
Biol Chem. 2023 Jun 27. doi: 10.1515/hsz-2023-0172. Online ahead of print.ABSTRACTNeuromuscular cell culture models are used to investigate synapse formation and function, as well as mechanisms of de-and regeneration in neuromuscular diseases. Recent developments including 3D culture technique and hiPSC technology have propelled their ability to complement insights from in vivo models. However, most cultures have not considered Schwann cells, the glial part of NMJs. In the following, a brief overview of different types of neuromuscular cocultures is provided alongside examples for studies that included Schwann cells. From ...
Source: Biological Chemistry - June 26, 2023 Category: Chemistry Authors: Sarah Janice H örner Nathalie Couturier Mathias Hafner R üdiger Rudolf Source Type: research
Two are not enough: synthetic strategies and applications of unnatural base pairs
Biol Chem. 2023 Jun 26. doi: 10.1515/hsz-2023-0169. Online ahead of print.ABSTRACTNucleic acid chemistry is a rapidly evolving field, and the need for novel nucleotide modifications and artificial nucleotide building blocks for diagnostic and therapeutic use, material science or for studying cellular processes continues unabated. This review focusses on the development and application of unnatural base pairs as part of an expanded genetic alphabet. Not only recent developments in "nature-like" artificial base pairs are presented, but also current synthetic methods to get access to C-glycosidic nucleotides. Wide-ranging via...
Source: Biological Chemistry - June 24, 2023 Category: Chemistry Authors: Robert D örrenhaus Philip K Wagner Stephanie Kath-Schorr Source Type: research
Structure and phase separation of the C-terminal domain of RNA polymerase II
Biol Chem. 2023 Jun 20. doi: 10.1515/hsz-2023-0136. Online ahead of print.ABSTRACTThe repetitive heptads in the C-terminal domain (CTD) of RPB1, the largest subunit of RNA Polymerase II (Pol II), play a critical role in the regulation of Pol II-based transcription. Recent findings on the structure of the CTD in the pre-initiation complex determined by cryo-EM and the novel phase separation properties of key transcription components offers an expanded mechanistic interpretation of the spatiotemporal distribution of Pol II during transcription. Current experimental evidence further suggests an exquisite balance between CTD's...
Source: Biological Chemistry - June 18, 2023 Category: Chemistry Authors: Irina P Lushpinskaia David Flores-Solis Markus Zweckstetter Source Type: research
