Intracellular spatially-targeted chemical chaperones increase native state stability of mutant SOD1 barrel
Biol Chem. 2023 Aug 9. doi: 10.1515/hsz-2023-0198. Online ahead of print.ABSTRACTAmyotrophic lateral sclerosis (ALS) is a progressive neurological disorder with currently no cure. Central to the cellular dysfunction associated with this fatal proteinopathy is the accumulation of unfolded/misfolded superoxide dismutase 1 (SOD1) in various subcellular locations. The molecular mechanism driving the formation of SOD1 aggregates is not fully understood but numerous studies suggest that aberrant aggregation escalates with folding instability of mutant apoSOD1. Recent advances on combining organelle-targeting therapies with the a...
Source: Biological Chemistry - August 9, 2023 Category: Chemistry Authors: Sara S Ribeiro David Gnutt Salome Azoulay-Ginsburg Zamira Fetahaj Ella Spurlock Felix Lindner Damon Kuz Yfat Cohen-Erez Hanna Rapaport Adrian Israelson Arie-Lev Gruzman Simon Ebbinghaus Source Type: research

Crosstalk between cholesterol and PIP < sub > 2 < /sub > in the regulation of Kv7.2/Kv7.3 channels
Biol Chem. 2023 Aug 8. doi: 10.1515/hsz-2023-0204. Online ahead of print.ABSTRACTThe activity of neuronal Kv7.2/Kv7.3 channels is critically dependent on PIP2 and finely modulated by cholesterol. Here, we report the crosstalk between cholesterol and PIP2 in the regulation of Kv7.2/Kv7.3 channels. Our results show that currents passing through Kv7.2/Kv7.3 channels in cholesterol-depleted cells, by acute application of methyl-β-cyclodextrin (MβCD), were less sensitive to PIP2 dephosphorylation strategies than those of control cells, suggesting that cholesterol depletion enhances the Kv7.2/Kv7.3-PIP2 interaction. In contras...
Source: Biological Chemistry - August 8, 2023 Category: Chemistry Authors: Mayra Delgado-Ram írez Ana Laura L ópez-Serrano Sergio S ánchez-Armass Ulises Meza Aldo A Rodr íguez-Menchaca Source Type: research

Highlight: integrative structural biology of dynamic macromolecular assemblies
Biol Chem. 2023 Aug 8. doi: 10.1515/hsz-2023-0256. Online ahead of print.NO ABSTRACTPMID:37549047 | DOI:10.1515/hsz-2023-0256 (Source: Biological Chemistry)
Source: Biological Chemistry - August 7, 2023 Category: Chemistry Authors: Ralf Ficner Source Type: research

Bayesian methods in integrative structure modeling
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0145. Online ahead of print.ABSTRACTThere is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular systems on a variety of length and time scales. These techniques range from imaging with light, X-rays or electrons, to spectroscopic methods, cross-linking mass spectrometry and functional genomics approaches, and are complemented by AI-assisted protein structure prediction methods. A challenge is to integrat...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Michael Habeck Source Type: research

The chromatin  - triple helix connection
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins,...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research

Bayesian methods in integrative structure modeling
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0145. Online ahead of print.ABSTRACTThere is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular systems on a variety of length and time scales. These techniques range from imaging with light, X-rays or electrons, to spectroscopic methods, cross-linking mass spectrometry and functional genomics approaches, and are complemented by AI-assisted protein structure prediction methods. A challenge is to integrat...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Michael Habeck Source Type: research

The chromatin  - triple helix connection
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins,...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research

Bayesian methods in integrative structure modeling
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0145. Online ahead of print.ABSTRACTThere is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular systems on a variety of length and time scales. These techniques range from imaging with light, X-rays or electrons, to spectroscopic methods, cross-linking mass spectrometry and functional genomics approaches, and are complemented by AI-assisted protein structure prediction methods. A challenge is to integrat...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Michael Habeck Source Type: research