Structure and phase separation of the C-terminal domain of RNA polymerase II

Biol Chem. 2023 Jun 20. doi: 10.1515/hsz-2023-0136. Online ahead of print.ABSTRACTThe repetitive heptads in the C-terminal domain (CTD) of RPB1, the largest subunit of RNA Polymerase II (Pol II), play a critical role in the regulation of Pol II-based transcription. Recent findings on the structure of the CTD in the pre-initiation complex determined by cryo-EM and the novel phase separation properties of key transcription components offers an expanded mechanistic interpretation of the spatiotemporal distribution of Pol II during transcription. Current experimental evidence further suggests an exquisite balance between CTD's local structure and an array of multivalent interactions that drive phase separation of Pol II and thus shape its transcriptional activity.PMID:37331973 | DOI:10.1515/hsz-2023-0136

Source: Biological Chemistry - June 18, 2023 Category: Chemistry Authors: Irina P Lushpinskaia David Flores-Solis Markus Zweckstetter Source Type: research