Cathepsin L-mediated EGFR cleavage affects intracellular signalling pathways in cancer
In this study, we demonstrated extracellular cathepsin L-mediated cleavage of epidermal growth factor receptor (EGFR) and identified the cleavage site in the extracellular domain after R224. To further evaluate the relevance of this cleavage, we cloned and expressed a truncated version of EGFR, starting at G225, in HeLa cells. We confirmed the constitutive activation of the truncated protein in the absence of ligand binding and determined possible changes in intracellular signalling. Furthermore, we determined the effect of truncated EGFR protein expression on HeLa cell viability and response to the EGFR inhibitors, tyrosi...
Source: Biological Chemistry - October 27, 2023 Category: Chemistry Authors: Marija Grozdani ć Barbara Soboti č Monika Biasizzo Tilen Sever Robert Vidmar Matej Vizovi šek Boris Turk Marko Fonovi ć Source Type: research
Features of yeast RNA polymerase I with special consideration of the lobe binding subunits
In this report we focus on the Pol I specific lobe binding subunits which support efficient, error-free, and correctly terminated rRNA synthesis.PMID:37823775 | DOI:10.1515/hsz-2023-0184 (Source: Biological Chemistry)
Source: Biological Chemistry - October 12, 2023 Category: Chemistry Authors: Katrin Schwank Catharina Schmid Tobias Fremter Christoph Engel Philipp Milkereit Joachim Griesenbeck Herbert Tschochner Source Type: research
