Post-transcriptional gene silencing in a dynamic RNP world
Biol Chem. 2023 Sep 25. doi: 10.1515/hsz-2023-0203. Online ahead of print.ABSTRACTMicroRNA (miRNA)-guided gene silencing is a key regulatory process in various organisms and linked to many human diseases. MiRNAs are processed from precursor molecules and associate with Argonaute proteins to repress the expression of complementary target mRNAs. Excellent work by numerous labs has contributed to a detailed understanding of the mechanisms of miRNA function. However, miRNA effects have mostly been analyzed and viewed as isolated events and their natural environment as part of complex RNA-protein particles (RNPs) is often negle...
Source: Biological Chemistry - September 22, 2023 Category: Chemistry Authors: Simone Larivera Julia Neumeier Gunter Meister Source Type: research
Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation
In conclusion, NO2OA exhibited potential for combined chemoradiotherapy. Our results encourage the development of new NFAs with improved features for cancer chemoradiation.PMID:37712609 | DOI:10.1515/hsz-2023-0150 (Source: Biological Chemistry)
Source: Biological Chemistry - September 15, 2023 Category: Chemistry Authors: Tomas Perecko Jana Pereckova Zuzana Hoferova Martin Falk Source Type: research
The archaeal Lsm protein from < em > Pyrococcus furiosus < /em > binds co-transcriptionally to poly(U)-rich target RNAs
Biol Chem. 2023 Sep 15. doi: 10.1515/hsz-2023-0215. Online ahead of print.ABSTRACTPosttranscriptional processes in Bacteria include the association of small regulatory RNAs (sRNA) with a target mRNA. The sRNA/mRNA annealing process is often mediated by an RNA chaperone called Hfq. The functional role of bacterial and eukaryotic Lsm proteins is partially understood, whereas knowledge about archaeal Lsm proteins is scarce. Here, we used the genetically tractable archaeal hyperthermophile Pyrococcus furiosus to identify the protein interaction partners of the archaeal Sm-like proteins (PfuSmAP1) using mass spectrometry and pe...
Source: Biological Chemistry - September 14, 2023 Category: Chemistry Authors: Robert Reichelt Tamara Rothmeier Felix Gr ünberger Sarah Willkomm Astrid Bruckmann Winfried Hausner Dina Grohmann Source Type: research
A structural biology view on the enzymes involved in eukaryotic mRNA turnover
Biol Chem. 2023 Sep 15. doi: 10.1515/hsz-2023-0182. Online ahead of print.ABSTRACTThe cellular environment contains numerous ribonucleases that are dedicated to process mRNA transcripts that have been targeted for degradation. Here, we review the three dimensional structures of the ribonuclease complexes (Pan2-Pan3, Ccr4-Not, Xrn1, exosome) and the mRNA decapping enzymes (Dcp2, DcpS) that are involved in mRNA turnover. Structures of major parts of these proteins have been experimentally determined. These enzymes and factors do not act in isolation, but are embedded in interaction networks which regulate enzyme activity and...
Source: Biological Chemistry - September 14, 2023 Category: Chemistry Authors: Christina Krempl Daniela Lazzaretti Remco Sprangers Source Type: research
The archaeal Lsm protein from < em > Pyrococcus furiosus < /em > binds co-transcriptionally to poly(U)-rich target RNAs
Biol Chem. 2023 Sep 15. doi: 10.1515/hsz-2023-0215. Online ahead of print.ABSTRACTPosttranscriptional processes in Bacteria include the association of small regulatory RNAs (sRNA) with a target mRNA. The sRNA/mRNA annealing process is often mediated by an RNA chaperone called Hfq. The functional role of bacterial and eukaryotic Lsm proteins is partially understood, whereas knowledge about archaeal Lsm proteins is scarce. Here, we used the genetically tractable archaeal hyperthermophile Pyrococcus furiosus to identify the protein interaction partners of the archaeal Sm-like proteins (PfuSmAP1) using mass spectrometry and pe...
Source: Biological Chemistry - September 14, 2023 Category: Chemistry Authors: Robert Reichelt Tamara Rothmeier Felix Gr ünberger Sarah Willkomm Astrid Bruckmann Winfried Hausner Dina Grohmann Source Type: research
A structural biology view on the enzymes involved in eukaryotic mRNA turnover
Biol Chem. 2023 Sep 15. doi: 10.1515/hsz-2023-0182. Online ahead of print.ABSTRACTThe cellular environment contains numerous ribonucleases that are dedicated to process mRNA transcripts that have been targeted for degradation. Here, we review the three dimensional structures of the ribonuclease complexes (Pan2-Pan3, Ccr4-Not, Xrn1, exosome) and the mRNA decapping enzymes (Dcp2, DcpS) that are involved in mRNA turnover. Structures of major parts of these proteins have been experimentally determined. These enzymes and factors do not act in isolation, but are embedded in interaction networks which regulate enzyme activity and...
Source: Biological Chemistry - September 14, 2023 Category: Chemistry Authors: Christina Krempl Daniela Lazzaretti Remco Sprangers Source Type: research
