The archaeal Lsm protein from < em > Pyrococcus furiosus < /em > binds co-transcriptionally to poly(U)-rich target RNAs
Biol Chem. 2023 Sep 15. doi: 10.1515/hsz-2023-0215. Online ahead of print.ABSTRACTPosttranscriptional processes in Bacteria include the association of small regulatory RNAs (sRNA) with a target mRNA. The sRNA/mRNA annealing process is often mediated by an RNA chaperone called Hfq. The functional role of bacterial and eukaryotic Lsm proteins is partially understood, whereas knowledge about archaeal Lsm proteins is scarce. Here, we used the genetically tractable archaeal hyperthermophile Pyrococcus furiosus to identify the protein interaction partners of the archaeal Sm-like proteins (PfuSmAP1) using mass spectrometry and pe...
Source: Biological Chemistry - September 14, 2023 Category: Chemistry Authors: Robert Reichelt Tamara Rothmeier Felix Gr ünberger Sarah Willkomm Astrid Bruckmann Winfried Hausner Dina Grohmann Source Type: research

A structural biology view on the enzymes involved in eukaryotic mRNA turnover
Biol Chem. 2023 Sep 15. doi: 10.1515/hsz-2023-0182. Online ahead of print.ABSTRACTThe cellular environment contains numerous ribonucleases that are dedicated to process mRNA transcripts that have been targeted for degradation. Here, we review the three dimensional structures of the ribonuclease complexes (Pan2-Pan3, Ccr4-Not, Xrn1, exosome) and the mRNA decapping enzymes (Dcp2, DcpS) that are involved in mRNA turnover. Structures of major parts of these proteins have been experimentally determined. These enzymes and factors do not act in isolation, but are embedded in interaction networks which regulate enzyme activity and...
Source: Biological Chemistry - September 14, 2023 Category: Chemistry Authors: Christina Krempl Daniela Lazzaretti Remco Sprangers Source Type: research

The archaeal Lsm protein from < em > Pyrococcus furiosus < /em > binds co-transcriptionally to poly(U)-rich target RNAs
Biol Chem. 2023 Sep 15. doi: 10.1515/hsz-2023-0215. Online ahead of print.ABSTRACTPosttranscriptional processes in Bacteria include the association of small regulatory RNAs (sRNA) with a target mRNA. The sRNA/mRNA annealing process is often mediated by an RNA chaperone called Hfq. The functional role of bacterial and eukaryotic Lsm proteins is partially understood, whereas knowledge about archaeal Lsm proteins is scarce. Here, we used the genetically tractable archaeal hyperthermophile Pyrococcus furiosus to identify the protein interaction partners of the archaeal Sm-like proteins (PfuSmAP1) using mass spectrometry and pe...
Source: Biological Chemistry - September 14, 2023 Category: Chemistry Authors: Robert Reichelt Tamara Rothmeier Felix Gr ünberger Sarah Willkomm Astrid Bruckmann Winfried Hausner Dina Grohmann Source Type: research

A structural biology view on the enzymes involved in eukaryotic mRNA turnover
Biol Chem. 2023 Sep 15. doi: 10.1515/hsz-2023-0182. Online ahead of print.ABSTRACTThe cellular environment contains numerous ribonucleases that are dedicated to process mRNA transcripts that have been targeted for degradation. Here, we review the three dimensional structures of the ribonuclease complexes (Pan2-Pan3, Ccr4-Not, Xrn1, exosome) and the mRNA decapping enzymes (Dcp2, DcpS) that are involved in mRNA turnover. Structures of major parts of these proteins have been experimentally determined. These enzymes and factors do not act in isolation, but are embedded in interaction networks which regulate enzyme activity and...
Source: Biological Chemistry - September 14, 2023 Category: Chemistry Authors: Christina Krempl Daniela Lazzaretti Remco Sprangers Source Type: research

The promise of genetic screens in human < em > in  vitro < /em > brain models
Biol Chem. 2023 Sep 12. doi: 10.1515/hsz-2023-0174. Online ahead of print.ABSTRACTAdvances of in vitro culture models have allowed unprecedented insights into human neurobiology. At the same time genetic screening has matured into a robust and accessible experimental strategy allowing for the simultaneous study of many genes in parallel. The combination of both technologies is a newly emerging tool for neuroscientists, opening the door to identifying causal cell- and tissue-specific developmental and disease mechanisms. However, with complex experimental genetic screening set-ups new challenges in data interpretation and e...
Source: Biological Chemistry - September 12, 2023 Category: Chemistry Authors: Julianne Beirute-Herrera Beatriz L ópez-Amo Calvo Frank Edenhofer Christopher Esk Source Type: research

N6-methyladenosine-induced METTL1 promotes tumor proliferation via CDK4
Biol Chem. 2023 Sep 12. doi: 10.1515/hsz-2023-0260. Online ahead of print.ABSTRACTN6-methyladenosine (m6A) and N7-methylguanosine (m7G) modification of RNA represent two major intracellular post-transcriptional regulation modes of gene expression. However, the crosstalk of these two epigenetic modifications in tumorigenesis remain poorly understood. Here, we show that m6A methyltransferase METTL3-mediated METTL1 promotes cell proliferation of head and neck squamous cell carcinoma (HNSC) through m7G modification of the cell-cycle regulator CDK4. By mining the database GEPIA, METTL1 was shown to be up-regulated in a broad sp...
Source: Biological Chemistry - September 11, 2023 Category: Chemistry Authors: Chunyan Zhang Yuanbo Cui Source Type: research

Microtubules as a signal hub for axon growth in response to mechanical force
Biol Chem. 2023 Sep 8. doi: 10.1515/hsz-2023-0173. Online ahead of print.ABSTRACTMicrotubules are highly polar structures and are characterized by high anisotropy and stiffness. In neurons, they play a key role in the directional transport of vesicles and organelles. In the neuronal projections called axons, they form parallel bundles, mostly oriented with the plus-end towards the axonal termination. Their physico-chemical properties have recently attracted attention as a potential candidate in sensing, processing and transducing physical signals generated by mechanical forces. Here, we discuss the main evidence supporting...
Source: Biological Chemistry - September 7, 2023 Category: Chemistry Authors: Alessandro Falconieri Allegra Coppini Vittoria Raffa Source Type: research

Cytosolic RGG RNA-binding proteins are temperature sensitive flowering time regulators in < em > Arabidopsis < /em >
In conclusion, we suggest that AtRGGs function in fine-tuning translation efficiency to control flowering time and potentially other developmental processes in response to environmental changes.PMID:37674329 | DOI:10.1515/hsz-2023-0171 (Source: Biological Chemistry)
Source: Biological Chemistry - September 7, 2023 Category: Chemistry Authors: Andrea Bleckmann Nicole Spitzlberger Philipp Denninger Hans F Ehrnsberger Lele Wang Astrid Bruckmann Stefan Reich Philipp Holzinger Jan Medenbach Klaus D Grasser Thomas Dresselhaus Source Type: research

The Zika virus infection remodels the expression of the synaptotagmin-9 secretory protein
Biol Chem. 2023 Sep 8. doi: 10.1515/hsz-2023-0165. Online ahead of print.ABSTRACTThe exact mechanisms involved in flaviviruses virions' release and the specific secretion of viral proteins, such as the Non Structural protein-1 (NS1), are still unclear. While these processes might involve vesicular transport to the cell membrane, NS1 from some flaviviruses was shown to participate in viral assembly and release. Here, we assessed the effect of the Zika virus (ZIKV) NS1 expression on the cellular proteome to identify trafficking-related targets that may be altered in the presence of the viral protein. We detected an increase ...
Source: Biological Chemistry - September 7, 2023 Category: Chemistry Authors: Santiago Leiva Alejo Cantoia Cintia Fabbri Marina Bugnon Valdano Victoria Luppo Mar ía Alejandra Morales Germ án Rosano Daniela Gardiol Source Type: research

Microtubules as a signal hub for axon growth in response to mechanical force
Biol Chem. 2023 Sep 8. doi: 10.1515/hsz-2023-0173. Online ahead of print.ABSTRACTMicrotubules are highly polar structures and are characterized by high anisotropy and stiffness. In neurons, they play a key role in the directional transport of vesicles and organelles. In the neuronal projections called axons, they form parallel bundles, mostly oriented with the plus-end towards the axonal termination. Their physico-chemical properties have recently attracted attention as a potential candidate in sensing, processing and transducing physical signals generated by mechanical forces. Here, we discuss the main evidence supporting...
Source: Biological Chemistry - September 7, 2023 Category: Chemistry Authors: Alessandro Falconieri Allegra Coppini Vittoria Raffa Source Type: research

Cytosolic RGG RNA-binding proteins are temperature sensitive flowering time regulators in < em > Arabidopsis < /em >
In conclusion, we suggest that AtRGGs function in fine-tuning translation efficiency to control flowering time and potentially other developmental processes in response to environmental changes.PMID:37674329 | DOI:10.1515/hsz-2023-0171 (Source: Biological Chemistry)
Source: Biological Chemistry - September 7, 2023 Category: Chemistry Authors: Andrea Bleckmann Nicole Spitzlberger Philipp Denninger Hans F Ehrnsberger Lele Wang Astrid Bruckmann Stefan Reich Philipp Holzinger Jan Medenbach Klaus D Grasser Thomas Dresselhaus Source Type: research

The Zika virus infection remodels the expression of the synaptotagmin-9 secretory protein
Biol Chem. 2023 Sep 8. doi: 10.1515/hsz-2023-0165. Online ahead of print.ABSTRACTThe exact mechanisms involved in flaviviruses virions' release and the specific secretion of viral proteins, such as the Non Structural protein-1 (NS1), are still unclear. While these processes might involve vesicular transport to the cell membrane, NS1 from some flaviviruses was shown to participate in viral assembly and release. Here, we assessed the effect of the Zika virus (ZIKV) NS1 expression on the cellular proteome to identify trafficking-related targets that may be altered in the presence of the viral protein. We detected an increase ...
Source: Biological Chemistry - September 7, 2023 Category: Chemistry Authors: Santiago Leiva Alejo Cantoia Cintia Fabbri Marina Bugnon Valdano Victoria Luppo Mar ía Alejandra Morales Germ án Rosano Daniela Gardiol Source Type: research

Microtubules as a signal hub for axon growth in response to mechanical force
Biol Chem. 2023 Sep 8. doi: 10.1515/hsz-2023-0173. Online ahead of print.ABSTRACTMicrotubules are highly polar structures and are characterized by high anisotropy and stiffness. In neurons, they play a key role in the directional transport of vesicles and organelles. In the neuronal projections called axons, they form parallel bundles, mostly oriented with the plus-end towards the axonal termination. Their physico-chemical properties have recently attracted attention as a potential candidate in sensing, processing and transducing physical signals generated by mechanical forces. Here, we discuss the main evidence supporting...
Source: Biological Chemistry - September 7, 2023 Category: Chemistry Authors: Alessandro Falconieri Allegra Coppini Vittoria Raffa Source Type: research