Detection of a 7SL RNA-derived small non-coding RNA using Molecular Beacons < em > in vitro < /em > and in cells
Biol Chem. 2023 Aug 28. doi: 10.1515/hsz-2023-0185. Online ahead of print.ABSTRACTSmall non-coding RNAs (sncRNA) are involved in many steps of the gene expression cascade and regulate processing and expression of mRNAs by the formation of ribonucleoprotein complexes (RNP) such as the RNA-induced silencing complex (RISC). By analyzing small RNA Seq data sets, we identified a sncRNA annotated as piR-hsa-1254, which is likely derived from the 3'-end of 7SL RNA2 (RN7SL2), herein referred to as snc7SL RNA. The 7SL RNA is an abundant long non-coding RNA polymerase III transcript and serves as structural component of the cytoplas...
Source: Biological Chemistry - August 26, 2023 Category: Chemistry Authors: Nina Weigert Anna-Lena Schweiger Jonas Gross Marie Matthes Selim Corbacioglu Gunhild Sommer Tilman Heise Source Type: research
Rodent models for mood disorders - understanding molecular changes by investigating social behavior
Biol Chem. 2023 Aug 28. doi: 10.1515/hsz-2023-0190. Online ahead of print.ABSTRACTMood disorders, including depressive and bipolar disorders, are the group of psychiatric disorders with the highest prevalence and disease burden. However, their pathophysiology remains poorly understood. Animal models are an extremely useful tool for the investigation of molecular mechanisms underlying these disorders. For psychiatric symptom assessment in animals, a meaningful behavioral phenotype is needed. Social behaviors constitute naturally occurring complex behaviors in rodents and can therefore serve as such a phenotype, contributing...
Source: Biological Chemistry - August 26, 2023 Category: Chemistry Authors: Patrick R Reinhardt Candy D C Theis Georg Juckel Nadja Freund Source Type: research
Detection of a 7SL RNA-derived small non-coding RNA using Molecular Beacons < em > in vitro < /em > and in cells
Biol Chem. 2023 Aug 28. doi: 10.1515/hsz-2023-0185. Online ahead of print.ABSTRACTSmall non-coding RNAs (sncRNA) are involved in many steps of the gene expression cascade and regulate processing and expression of mRNAs by the formation of ribonucleoprotein complexes (RNP) such as the RNA-induced silencing complex (RISC). By analyzing small RNA Seq data sets, we identified a sncRNA annotated as piR-hsa-1254, which is likely derived from the 3'-end of 7SL RNA2 (RN7SL2), herein referred to as snc7SL RNA. The 7SL RNA is an abundant long non-coding RNA polymerase III transcript and serves as structural component of the cytoplas...
Source: Biological Chemistry - August 26, 2023 Category: Chemistry Authors: Nina Weigert Anna-Lena Schweiger Jonas Gross Marie Matthes Selim Corbacioglu Gunhild Sommer Tilman Heise Source Type: research
Rodent models for mood disorders - understanding molecular changes by investigating social behavior
Biol Chem. 2023 Aug 28. doi: 10.1515/hsz-2023-0190. Online ahead of print.ABSTRACTMood disorders, including depressive and bipolar disorders, are the group of psychiatric disorders with the highest prevalence and disease burden. However, their pathophysiology remains poorly understood. Animal models are an extremely useful tool for the investigation of molecular mechanisms underlying these disorders. For psychiatric symptom assessment in animals, a meaningful behavioral phenotype is needed. Social behaviors constitute naturally occurring complex behaviors in rodents and can therefore serve as such a phenotype, contributing...
Source: Biological Chemistry - August 26, 2023 Category: Chemistry Authors: Patrick R Reinhardt Candy D C Theis Georg Juckel Nadja Freund Source Type: research
Detection of a 7SL RNA-derived small non-coding RNA using Molecular Beacons < em > in vitro < /em > and in cells
Biol Chem. 2023 Aug 28. doi: 10.1515/hsz-2023-0185. Online ahead of print.ABSTRACTSmall non-coding RNAs (sncRNA) are involved in many steps of the gene expression cascade and regulate processing and expression of mRNAs by the formation of ribonucleoprotein complexes (RNP) such as the RNA-induced silencing complex (RISC). By analyzing small RNA Seq data sets, we identified a sncRNA annotated as piR-hsa-1254, which is likely derived from the 3'-end of 7SL RNA2 (RN7SL2), herein referred to as snc7SL RNA. The 7SL RNA is an abundant long non-coding RNA polymerase III transcript and serves as structural component of the cytoplas...
Source: Biological Chemistry - August 26, 2023 Category: Chemistry Authors: Nina Weigert Anna-Lena Schweiger Jonas Gross Marie Matthes Selim Corbacioglu Gunhild Sommer Tilman Heise Source Type: research
Characterization of the active site in the thiocyanate-forming protein from < em > Thlaspi arvense < /em > (TaTFP) using EPR spectroscopy
Biol Chem. 2023 Aug 17. doi: 10.1515/hsz-2023-0187. Online ahead of print.ABSTRACTGlucosinolates are plant thioglucosides, which act as chemical defenses. Upon tissue damage, their myrosinase-catalyzed hydrolysis yields aglucones that rearrange to toxic isothiocyanates. Specifier proteins such as thiocyanate-forming protein from Thlaspi arvense (TaTFP) are non-heme iron proteins, which capture the aglucone to form alternative products, e.g. nitriles or thiocyanates. To resolve the electronic state of the bound iron cofactor in TaTFP, we applied continuous wave electron paramagnetic resonance (CW EPR) spectroscopy at X-and ...
Source: Biological Chemistry - August 16, 2023 Category: Chemistry Authors: Haleh Hashemi Haeri Nicola Schneegans Daniela Eisenschmidt-B önn Wolfgang Brandt Ute Wittstock Dariush Hinderberger Source Type: research
Intracellular spatially-targeted chemical chaperones increase native state stability of mutant SOD1 barrel
Biol Chem. 2023 Aug 9. doi: 10.1515/hsz-2023-0198. Online ahead of print.ABSTRACTAmyotrophic lateral sclerosis (ALS) is a progressive neurological disorder with currently no cure. Central to the cellular dysfunction associated with this fatal proteinopathy is the accumulation of unfolded/misfolded superoxide dismutase 1 (SOD1) in various subcellular locations. The molecular mechanism driving the formation of SOD1 aggregates is not fully understood but numerous studies suggest that aberrant aggregation escalates with folding instability of mutant apoSOD1. Recent advances on combining organelle-targeting therapies with the a...
Source: Biological Chemistry - August 9, 2023 Category: Chemistry Authors: Sara S Ribeiro David Gnutt Salome Azoulay-Ginsburg Zamira Fetahaj Ella Spurlock Felix Lindner Damon Kuz Yfat Cohen-Erez Hanna Rapaport Adrian Israelson Arie-Lev Gruzman Simon Ebbinghaus Source Type: research
