The chromatin - triple helix connection
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins,...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research
Bayesian methods in integrative structure modeling
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0145. Online ahead of print.ABSTRACTThere is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular systems on a variety of length and time scales. These techniques range from imaging with light, X-rays or electrons, to spectroscopic methods, cross-linking mass spectrometry and functional genomics approaches, and are complemented by AI-assisted protein structure prediction methods. A challenge is to integrat...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Michael Habeck Source Type: research
The chromatin - triple helix connection
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins,...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research
Bayesian methods in integrative structure modeling
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0145. Online ahead of print.ABSTRACTThere is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular systems on a variety of length and time scales. These techniques range from imaging with light, X-rays or electrons, to spectroscopic methods, cross-linking mass spectrometry and functional genomics approaches, and are complemented by AI-assisted protein structure prediction methods. A challenge is to integrat...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Michael Habeck Source Type: research
The chromatin - triple helix connection
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins,...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research
Bayesian methods in integrative structure modeling
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0145. Online ahead of print.ABSTRACTThere is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular systems on a variety of length and time scales. These techniques range from imaging with light, X-rays or electrons, to spectroscopic methods, cross-linking mass spectrometry and functional genomics approaches, and are complemented by AI-assisted protein structure prediction methods. A challenge is to integrat...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Michael Habeck Source Type: research
The chromatin - triple helix connection
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins,...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research
Bayesian methods in integrative structure modeling
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0145. Online ahead of print.ABSTRACTThere is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular systems on a variety of length and time scales. These techniques range from imaging with light, X-rays or electrons, to spectroscopic methods, cross-linking mass spectrometry and functional genomics approaches, and are complemented by AI-assisted protein structure prediction methods. A challenge is to integrat...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Michael Habeck Source Type: research
The chromatin - triple helix connection
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins,...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research
Bayesian methods in integrative structure modeling
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0145. Online ahead of print.ABSTRACTThere is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular systems on a variety of length and time scales. These techniques range from imaging with light, X-rays or electrons, to spectroscopic methods, cross-linking mass spectrometry and functional genomics approaches, and are complemented by AI-assisted protein structure prediction methods. A challenge is to integrat...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Michael Habeck Source Type: research
The chromatin - triple helix connection
Biol Chem. 2023 Jul 31. doi: 10.1515/hsz-2023-0189. Online ahead of print.ABSTRACTMammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins,...
Source: Biological Chemistry - July 28, 2023 Category: Chemistry Authors: Rodrigo Maldonado Gernot L ängst Source Type: research
Synthesis of the ribosomal RNA precursor in human cells: mechanisms, factors and regulation
Biol Chem. 2023 Jul 17. doi: 10.1515/hsz-2023-0214. Online ahead of print.ABSTRACTThe ribosomal RNA precursor (pre-rRNA) comprises three of the four ribosomal RNAs and is synthesized by RNA polymerase (Pol) I. Here, we describe the mechanisms of Pol I transcription in human cells with a focus on recent insights gained from structure-function analyses. The comparison of Pol I-specific structural and functional features with those of other Pols and with the excessively studied yeast system distinguishes organism-specific from general traits. We explain the organization of the genomic rDNA loci in human cells, describe the Po...
Source: Biological Chemistry - July 16, 2023 Category: Chemistry Authors: Julia L Dai ß Joachim Griesenbeck Herbert Tschochner Christoph Engel Source Type: research
