Archives of Biochemistry and Biophysics This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Insights into membrane interactions and their therapeutic potential
Arch Biochem Biophys. 2024 Feb 20:109939. doi: 10.1016/j.abb.2024.109939. Online ahead of print.ABSTRACTRecent research into membrane interactions has uncovered a diverse range of therapeutic opportunities through the bioengineering of human and non-human macromolecules. Although the majority of this research is focussed on fundamental developments, emerging studies are showcasing promising new technologies to combat conditions such as cancer, Alzheimer's and inflammatory and immune-based disease, utilising the alteration of bacteriophage, adenovirus, bacterial toxins, type 6 secretion systems, annexins, mitochondrial anti...
Source: Archives of Biochemistry and Biophysics - February 22, 2024 Category: Biochemistry Authors: Calum Upton Joseph Healey Alice J Rothnie Alan D Goddard Source Type: research

Dye-decolorizing peroxidase of Thermobifida halotolerance displays complex kinetics with both substrate inhibition and apparent positive cooperativity
Arch Biochem Biophys. 2024 Feb 19:109931. doi: 10.1016/j.abb.2024.109931. Online ahead of print.ABSTRACTDye-decolorizing peroxidases (DyPs) have been intensively investigated for the purpose of industrial dye decolorization and lignin degradation. Unfortunately, the characterization of these peroxidases is hampered by their non-Michaelis-Menten kinetics, exemplified by substrate inhibition and/or positive cooperativity. Although often observed, the underlying mechanisms behind the unusual kinetics of DyPs are poorly understood. Here we studied the kinetics of the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfoni...
Source: Archives of Biochemistry and Biophysics - February 21, 2024 Category: Biochemistry Authors: Hegne Pupart Tiit Lukk Priit V äljamäe Source Type: research

The catalytic action of human d-lactate dehydrogenase is severely inhibited by oxalate and is impaired by mutations triggering d-lactate acidosis
Arch Biochem Biophys. 2024 Feb 17;754:109932. doi: 10.1016/j.abb.2024.109932. Online ahead of print.ABSTRACTd-lactate dehydrogenases are known to be expressed by prokaryotes and by eukaryotic invertebrates, and over the years the functional and structural features of some bacterial representatives of this enzyme ensemble have been investigated quite in detail. Remarkably, a human gene coding for a putative d-lactate dehydrogenase (DLDH) was identified and characterized, disclosing the occurrence of alternative splicing of its primary transcript. This translates into the expression of two human DLDH (hDLDH) isoforms, the mo...
Source: Archives of Biochemistry and Biophysics - February 19, 2024 Category: Biochemistry Authors: Alessandra Stefan Alberto Mucchi Alejandro Hochkoeppler Source Type: research

The catalytic action of human d-lactate dehydrogenase is severely inhibited by oxalate and is impaired by mutations triggering d-lactate acidosis
Arch Biochem Biophys. 2024 Feb 17:109932. doi: 10.1016/j.abb.2024.109932. Online ahead of print.ABSTRACTd-lactate dehydrogenases are known to be expressed by prokaryotes and by eukaryotic invertebrates, and over the years the functional and structural features of some bacterial representatives of this enzyme ensemble have been investigated quite in detail. Remarkably, a human gene coding for a putative d-lactate dehydrogenase (DLDH) was identified and characterized, disclosing the occurrence of alternative splicing of its primary transcript. This translates into the expression of two human DLDH (hDLDH) isoforms, the molecu...
Source: Archives of Biochemistry and Biophysics - February 19, 2024 Category: Biochemistry Authors: Alessandra Stefan Alberto Mucchi Alejandro Hochkoeppler Source Type: research

tRNA-derived fragments: Key determinants of cancer metastasis with emerging therapeutic and diagnostic potentials
Arch Biochem Biophys. 2024 Feb 16:109930. doi: 10.1016/j.abb.2024.109930. Online ahead of print.ABSTRACTMetastasis is a significant clinical challenge responsible for cancer mortality and non-response to treatment. However, the molecular mechanisms driving metastasis remain unclear, limiting the development of efficient diagnostic and therapeutic approaches. Recent breakthroughs in cancer biology have discovered a group of small non-coding RNAs called tRNA-derived fragments (tRFs), which play a critical role in the metastatic behavior of various tumors. tRFs are produced from cleavage modifications of tRNAs and have differ...
Source: Archives of Biochemistry and Biophysics - February 18, 2024 Category: Biochemistry Authors: Mohammad Salehi Mohammad Javad Kamali Aliakbar Rajabzadeh Shima Minoo Hamidreza Mosharafi Fatemeh Saeedi Abdolreza Daraei Source Type: research

Dihydrocelastrol induces cell death and suppresses angiogenesis through BCR/AP-1/junb signalling in diffuse large B cell lymphoma
Arch Biochem Biophys. 2024 Feb 15:109929. doi: 10.1016/j.abb.2024.109929. Online ahead of print.ABSTRACTDiffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma. Although treatment options have improved, a large proportion of patients show low survival rates, highlighting an urgent need for novel therapeutic strategies. The aim of this study was to investigate the efficacy of the new small-molecule compound dihydrocelastrol (DHCE), acquired through the structural modification of celastrol (CE), in the treatment of DLBCL. DHCE showed potent anti-lymphoma efficacy and synergistic effects with dox...
Source: Archives of Biochemistry and Biophysics - February 17, 2024 Category: Biochemistry Authors: Yue Lai Shushan Guo Qiongwei Tang Gaomei Chang Hui Zhang Bo Li Qilin Feng Ke Hu Zhijian Xu Xuejie Gao Qikai Zhang Hongfei Yi Dongliang Song Yifei Zhang Yu Peng Haiyan Cai Weiliang Zhu Jumei Shi Source Type: research

Involvement of the Keap1-Nrf2-ARE pathway in the antioxidant activity of sinomenine
This study is aimed to investigate the critical role of the nuclear factor erythroid 2-related factor 2 (Nrf2)-kelch-like ECH-associated protein-1(Keap1)-antioxidant response element (ARE) antioxidative signaling pathway in protecting sinomenine against H2O2-induced oxidative injury. Cytotoxicity and antioxidant experiments to initially determine the protective effects of sinomenine show that sinomenine has no effect on the decreased cell viability and presents similar potency in scavenging all three free radicals. The binding affinity between sinomenine and Keap1 was determined via fluorescence polarization assay, with IC...
Source: Archives of Biochemistry and Biophysics - February 14, 2024 Category: Biochemistry Authors: Tianzhu Guan Ning Li Xiangxiu Xu Dan Xiong Bo Wang Lixia Xiao Weikang Yang Ganghui Chu Abdulla Yusuf Jie Zhang Weiming Yue Source Type: research

Interrogating l-fuconate dehydratase with tartronate and 3-hydroxypyruvate reveals subtle differences within the mandelate racemase-subgroup of the enolase superfamily
We report a circular dichroism-based assay of FucD activity that monitors the change in ellipticity at 216 nm (Δ[Θ]S-P = 8985 ± 87 deg cm2 mol-1) accompanying the conversion of l-fuconate to 2-keto-3-deoxy-l-fuconate. Tartronate was a linear mixed-type inhibitor of FucD (Ki = 8.4 ± 0.7 mM, αKi = 63 ± 11 mM), binding 18-fold weaker than l-fuconate, compared with 2-fold weaker binding of tartronate by MR relative to mandelate. 3-HP irreversibly inactivated FucD (kinact/KI = 0.018 ± 0.002 M-1s-1) with an efficiency that was ∼4.6 × 102-fold less than that observed with MR. The inactivation arose predominantly from mo...
Source: Archives of Biochemistry and Biophysics - February 14, 2024 Category: Biochemistry Authors: Laura C McGary Christopher M Fetter Minglu Gu Meghan C Hamilton Himank Kumar Oliver P Kuehm Colin D Douglas Stephen L Bearne Source Type: research

Hyperglycemia - A culprit of podocyte pathology in the context of glycogen metabolism
Arch Biochem Biophys. 2024 Feb 11:109927. doi: 10.1016/j.abb.2024.109927. Online ahead of print.ABSTRACTProlonged disruption in the balance of glucose can result in metabolic disorders. The kidneys play a significant role in regulating blood glucose levels. However, when exposed to chronic hyperglycemia, the kidneys' ability to handle glucose metabolism may be impaired, leading to an accumulation of glycogen. Earlier studies have shown that there can be a significant increase in glucose storage in the form of glycogen in the kidneys in diabetes. Podocytes play a crucial role in maintaining the integrity of filtration barri...
Source: Archives of Biochemistry and Biophysics - February 13, 2024 Category: Biochemistry Authors: Olga Żołnierkiewicz Dorota Rogacka Source Type: research

Molecular basis of ligand recognition specificity of flavone glucosyltransferases in Nemophila menziesii
Arch Biochem Biophys. 2024 Feb 10:109926. doi: 10.1016/j.abb.2024.109926. Online ahead of print.ABSTRACTOf the more than 100 families of glycosyltransferases, family 1 glycosyltransferases catalyze glycosylation using uridine diphosphate (UDP)-sugar as a sugar donor and are thus referred to as UDP-sugar:glycosyl transferases. The blue color of the Nemophila menziesii flower is derived from metalloanthocyanin, which consists of anthocyanin, flavone, and metal ions. Flavone 7-O-β-glucoside-4'-O-β-glucoside in the plant is sequentially biosynthesized from flavons by UDP-glucose:flavone 4'-O-glucosyltransferase (NmF4'GT) and...
Source: Archives of Biochemistry and Biophysics - February 12, 2024 Category: Biochemistry Authors: Kazutaka Murayama Miyuki Kato-Murayama Toshiaki Hosaka Naoko Okitsu Yoshikazu Tanaka Mikako Shirouzu Source Type: research