Erythrocyte glutathione transferase. A sensitive Up-Down biomarker of environmental and industrial pollution
Arch Biochem Biophys. 2023 Oct 13:109786. doi: 10.1016/j.abb.2023.109786. Online ahead of print.ABSTRACTErythrocyte glutathione transferase is a well-known biomarker of environmental pollution. Examination of the extensive scientific literature discovers an atypical and very interesting property of this enzyme which may reveal a chronic exposition to many contaminants but in some cases even an acute and short-term dangerous contamination. This review also underlines the peculiar molecular and kinetic properties of this enzyme which makes it unique in the panorama of enzymes used as biomarker for environmental contamination...
Source: Archives of Biochemistry and Biophysics - October 15, 2023 Category: Biochemistry Authors: Alessio Bocedi Giorgia Gambardella Giada Cattani Sara Notari Giorgio Ricci Source Type: research

Evaluation of the antibacterial and inhibitory activity of the MepA efflux pump of Staphylococcus aureus by riparins I, II, III, and IV
Arch Biochem Biophys. 2023 Oct 13:109782. doi: 10.1016/j.abb.2023.109782. Online ahead of print.ABSTRACTThe efflux pump mechanism contributes to the antibiotic resistance of widely distributed strains of Staphylococcus aureus. Therefore, in the present work, the ability of the riparins N-(4-methoxyphenethyl)benzamide (I), 2-hydroxy-N-[2-(4-methoxyphenyl)ethyl]benzamide (II), 2, 6-dihydroxy-N-[ 2-(4-methoxyphenyl)ethyl]benzamide (III), and 3,4,5-trimethoxy-N-[2-(4-methoxyphenethyl)benzamide (IV) as potential inhibitors of the MepA efflux pump in S. aureus K2068 (fluoroquinolone-resistant). In addition, we performed checkerb...
Source: Archives of Biochemistry and Biophysics - October 15, 2023 Category: Biochemistry Authors: Cristina Rodrigues Dos Santos Barbosa Nair Silva Mac êdo Zildene de Sousa Silveira Jana ína Esmeraldo Rocha Thiago Sampaio Freitas D ébora Feitosa Muniz Isaac Moura Ara újo C ícera Datiane de Morais Oliveira-Tintino Emmanuel Silva Marinho Matheus Nun Source Type: research

Erythrocyte glutathione transferase. A sensitive Up-Down biomarker of environmental and industrial pollution
Arch Biochem Biophys. 2023 Oct 13:109786. doi: 10.1016/j.abb.2023.109786. Online ahead of print.ABSTRACTErythrocyte glutathione transferase is a well-known biomarker of environmental pollution. Examination of the extensive scientific literature discovers an atypical and very interesting property of this enzyme which may reveal a chronic exposition to many contaminants but in some cases even an acute and short-term dangerous contamination. This review also underlines the peculiar molecular and kinetic properties of this enzyme which makes it unique in the panorama of enzymes used as biomarker for environmental contamination...
Source: Archives of Biochemistry and Biophysics - October 15, 2023 Category: Biochemistry Authors: Alessio Bocedi Giorgia Gambardella Giada Cattani Sara Notari Giorgio Ricci Source Type: research

Evaluation of the antibacterial and inhibitory activity of the MepA efflux pump of Staphylococcus aureus by riparins I, II, III, and IV
Arch Biochem Biophys. 2023 Oct 13:109782. doi: 10.1016/j.abb.2023.109782. Online ahead of print.ABSTRACTThe efflux pump mechanism contributes to the antibiotic resistance of widely distributed strains of Staphylococcus aureus. Therefore, in the present work, the ability of the riparins N-(4-methoxyphenethyl)benzamide (I), 2-hydroxy-N-[2-(4-methoxyphenyl)ethyl]benzamide (II), 2, 6-dihydroxy-N-[ 2-(4-methoxyphenyl)ethyl]benzamide (III), and 3,4,5-trimethoxy-N-[2-(4-methoxyphenethyl)benzamide (IV) as potential inhibitors of the MepA efflux pump in S. aureus K2068 (fluoroquinolone-resistant). In addition, we performed checkerb...
Source: Archives of Biochemistry and Biophysics - October 15, 2023 Category: Biochemistry Authors: Cristina Rodrigues Dos Santos Barbosa Nair Silva Mac êdo Zildene de Sousa Silveira Jana ína Esmeraldo Rocha Thiago Sampaio Freitas D ébora Feitosa Muniz Isaac Moura Ara újo C ícera Datiane de Morais Oliveira-Tintino Emmanuel Silva Marinho Matheus Nun Source Type: research

Dihydropyrmidine dehydrogenase from Escherichia coli: Transient state analysis reveals both reductive activation prior to turnover and diminished substrate effector roles relative to the mammalian form
Arch Biochem Biophys. 2023 Oct 9:109772. doi: 10.1016/j.abb.2023.109772. Online ahead of print.ABSTRACTDihydropyrimidine dehydrogenase (DPD) is an enzyme that uses an elaborate architecture to catalyze a simple net reaction: the reduction of the vinylic bond of uracil and thymine. Known DPDs have two active sites separated by approximately 60 Å. One active site has an FAD cofactor and binds NAD(P) and the other has an FMN cofactor and binds pyrimidines. The intervening distance is spanned by four Fe4S4 centers that act as an electron conduit. Recent advancements with porcine DPD have revealed unexpected chemical sequences...
Source: Archives of Biochemistry and Biophysics - October 11, 2023 Category: Biochemistry Authors: Tyler B Alt Matthew R Hoag Graham R Moran Source Type: research

Dihydropyrmidine dehydrogenase from Escherichia coli: Transient state analysis reveals both reductive activation prior to turnover and diminished substrate effector roles relative to the mammalian form
Arch Biochem Biophys. 2023 Oct 9:109772. doi: 10.1016/j.abb.2023.109772. Online ahead of print.ABSTRACTDihydropyrimidine dehydrogenase (DPD) is an enzyme that uses an elaborate architecture to catalyze a simple net reaction: the reduction of the vinylic bond of uracil and thymine. Known DPDs have two active sites separated by approximately 60 Å. One active site has an FAD cofactor and binds NAD(P) and the other has an FMN cofactor and binds pyrimidines. The intervening distance is spanned by four Fe4S4 centers that act as an electron conduit. Recent advancements with porcine DPD have revealed unexpected chemical sequences...
Source: Archives of Biochemistry and Biophysics - October 11, 2023 Category: Biochemistry Authors: Tyler B Alt Matthew R Hoag Graham R Moran Source Type: research

Dihydropyrmidine dehydrogenase from Escherichia coli: Transient state analysis reveals both reductive activation prior to turnover and diminished substrate effector roles relative to the mammalian form
Arch Biochem Biophys. 2023 Oct 9:109772. doi: 10.1016/j.abb.2023.109772. Online ahead of print.ABSTRACTDihydropyrimidine dehydrogenase (DPD) is an enzyme that uses an elaborate architecture to catalyze a simple net reaction: the reduction of the vinylic bond of uracil and thymine. Known DPDs have two active sites separated by approximately 60 Å. One active site has an FAD cofactor and binds NAD(P) and the other has an FMN cofactor and binds pyrimidines. The intervening distance is spanned by four Fe4S4 centers that act as an electron conduit. Recent advancements with porcine DPD have revealed unexpected chemical sequences...
Source: Archives of Biochemistry and Biophysics - October 11, 2023 Category: Biochemistry Authors: Tyler B Alt Matthew R Hoag Graham R Moran Source Type: research

Dihydropyrmidine dehydrogenase from Escherichia coli: Transient state analysis reveals both reductive activation prior to turnover and diminished substrate effector roles relative to the mammalian form
Arch Biochem Biophys. 2023 Oct 9:109772. doi: 10.1016/j.abb.2023.109772. Online ahead of print.ABSTRACTDihydropyrimidine dehydrogenase (DPD) is an enzyme that uses an elaborate architecture to catalyze a simple net reaction: the reduction of the vinylic bond of uracil and thymine. Known DPDs have two active sites separated by approximately 60 Å. One active site has an FAD cofactor and binds NAD(P) and the other has an FMN cofactor and binds pyrimidines. The intervening distance is spanned by four Fe4S4 centers that act as an electron conduit. Recent advancements with porcine DPD have revealed unexpected chemical sequences...
Source: Archives of Biochemistry and Biophysics - October 11, 2023 Category: Biochemistry Authors: Tyler B Alt Matthew R Hoag Graham R Moran Source Type: research

Role of exosomes in prostate cancer bone metastasis
Arch Biochem Biophys. 2023 Oct 8;748:109784. doi: 10.1016/j.abb.2023.109784. Online ahead of print.ABSTRACTBone is a preferred metastatic site of prostate cancer (PCa), and most patients with PCa metastases develop osteogenic bone metastasis, which manifests as disturbed bone structure and poor bone quality. However, the underlying mechanisms of PCa bone metastasis remain unclear. In recent years, increasing evidence has implicated extracellular vesicles, especially exosomes, in PCa bone metastasis. Exosomes are 30-150 nm in diameter, enclosing a cargo of biomolecules, such as DNA, RNA, and proteins. Exosomes play a functi...
Source: Archives of Biochemistry and Biophysics - October 10, 2023 Category: Biochemistry Authors: Tingting Lv Zijie Li Dehua Wang Xiaojin Guo Xiaokuan Zhang Jing Cao Zhiyu Wang Source Type: research

LncRNA UCA1 promotes pancreatic cancer cell migration by regulating mitochondrial dynamics via the MAPK pathway
CONCLUSION: UCA1 promotes the migration by regulating the mitochondrial dynamics of pancreatic cancer cells via the MAPK/ERK pathway. Our findings suggest that UCA1 may serve as a potential biomarker in pancreatic cancer prognosis.PMID:37816421 | DOI:10.1016/j.abb.2023.109783 (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - October 10, 2023 Category: Biochemistry Authors: Huizhi Wang Yuntao Ding Yuxin He Zhengyue Yu Yujing Zhou Aihua Gong Min Xu Source Type: research

FAM134B-induced endoplasmic reticulum (ER)-phagy exacerbates cisplatin-insulted hair cell apoptosis :Possible relation to excessive ER stress
CONCLUSIONS: Collectively, the findings of this study demonstrate that FAM134B-mediated ER-phagy enhances the susceptibility of HCs to ER stress and apoptosis in response to cisplatin-induced stress. This suggests a sequential progression of ER-phagy, ER stress and apoptosis following cisplatin stimulus, and implies the potential therapeutic benefit of inhibiting of FAM134B-mediated ER-phagy in the prevention of cisplatin-related ototoxicity.PMID:37813237 | DOI:10.1016/j.abb.2023.109766 (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - October 9, 2023 Category: Biochemistry Authors: Huiming Yang Haiyan Yin Yue Wang Jisheng Liu Lingchuan Guo Hao Zhao Xiaohui Bai Jianfeng Li Qianqian Yang Source Type: research

FAM134B-induced endoplasmic reticulum (ER)-phagy exacerbates cisplatin-insulted hair cell apoptosis :Possible relation to excessive ER stress
CONCLUSIONS: Collectively, the findings of this study demonstrate that FAM134B-mediated ER-phagy enhances the susceptibility of HCs to ER stress and apoptosis in response to cisplatin-induced stress. This suggests a sequential progression of ER-phagy, ER stress and apoptosis following cisplatin stimulus, and implies the potential therapeutic benefit of inhibiting of FAM134B-mediated ER-phagy in the prevention of cisplatin-related ototoxicity.PMID:37813237 | DOI:10.1016/j.abb.2023.109766 (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - October 9, 2023 Category: Biochemistry Authors: Huiming Yang Haiyan Yin Yue Wang Jisheng Liu Lingchuan Guo Hao Zhao Xiaohui Bai Jianfeng Li Qianqian Yang Source Type: research

Losartan alleviates renal fibrosis by inhibiting the biomechanical stress-induced epithelial-mesenchymal transition of renal epithelial cells
In this study, we investigated the effectiveness and relevant mechanism of ARBs in alleviating renal fibrosis, especially by focusing on biomechanical stress-induced epithelial to mesenchymal transition (EMT) of renal epithelial cells. Unilateral ureteral obstruction (UUO) renal fibrosis model was established in mice by ligating the left ureter, and then randomly received losartan at a low dose (1 mg/kg) or a regular dose (3 mg/kg) for 2 weeks. Compared to the control, histological analysis showed that losartan treatment at either a low dose or a regular dose effectively attenuated renal fibrosis in the UUO model. To furth...
Source: Archives of Biochemistry and Biophysics - October 2, 2023 Category: Biochemistry Authors: Zisheng Huang Han Nie Geng Liu Peilin Li Yong-Hua Peng Jie Xiao Weili Gu Tao-Sheng Li Source Type: research

Losartan alleviates renal fibrosis by inhibiting the biomechanical stress-induced epithelial-mesenchymal transition of renal epithelial cells
In this study, we investigated the effectiveness and relevant mechanism of ARBs in alleviating renal fibrosis, especially by focusing on biomechanical stress-induced epithelial to mesenchymal transition (EMT) of renal epithelial cells. Unilateral ureteral obstruction (UUO) renal fibrosis model was established in mice by ligating the left ureter, and then randomly received losartan at a low dose (1 mg/kg) or a regular dose (3 mg/kg) for 2 weeks. Compared to the control, histological analysis showed that losartan treatment at either a low dose or a regular dose effectively attenuated renal fibrosis in the UUO model. To furth...
Source: Archives of Biochemistry and Biophysics - October 2, 2023 Category: Biochemistry Authors: Zisheng Huang Han Nie Geng Liu Peilin Li Yong-Hua Peng Jie Xiao Weili Gu Tao-Sheng Li Source Type: research

Losartan alleviates renal fibrosis by inhibiting the biomechanical stress-induced epithelial-mesenchymal transition of renal epithelial cells
In this study, we investigated the effectiveness and relevant mechanism of ARBs in alleviating renal fibrosis, especially by focusing on biomechanical stress-induced epithelial to mesenchymal transition (EMT) of renal epithelial cells. Unilateral ureteral obstruction (UUO) renal fibrosis model was established in mice by ligating the left ureter, and then randomly received losartan at a low dose (1 mg/kg) or a regular dose (3 mg/kg) for 2 weeks. Compared to the control, histological analysis showed that losartan treatment at either a low dose or a regular dose effectively attenuated renal fibrosis in the UUO model. To furth...
Source: Archives of Biochemistry and Biophysics - October 2, 2023 Category: Biochemistry Authors: Zisheng Huang Han Nie Geng Liu Peilin Li Yong-Hua Peng Jie Xiao Weili Gu Tao-Sheng Li Source Type: research