Archives of Biochemistry and Biophysics This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

P2Y1 and P2Y2 receptors differ in their role in the regulation of signaling pathways during unloading-induced rat soleus muscle atrophy
This study revealed several key findings following three days of soleus muscle unloading: 1: Inhibition of P2Y1 or P2Y2 receptors prevented the accumulation of ATP, the increase in IP3 receptor content, and the decrease in the phosphorylation of GSK-3beta. This inhibition also mitigated the reduction in the rate of protein synthesis. However, it had no significant effect on the markers of mTORC1-dependent signaling. 2: Blocking P2Y1 receptors prevented the unloading-induced upregulation of phosphorylated p38MAPK and partially reduced the increase in MuRF1mRNA expression. 3: Blocking P2Y2 receptors prevented muscle atrophy ...
Source: Archives of Biochemistry and Biophysics - December 3, 2023 Category: Biochemistry Authors: Ksenia A Zaripova Svetlana P Belova Tatiana Y Kostrominova Boris S Shenkman Tatiana L Nemirovskaya Source Type: research

Menadione and protocatechuic acid: A drug combination with antitumor effects in murine osteosarcoma cells
This study evaluated the cytotoxic and anticancer effects of the association between menadione (MEN) and protocatechuic acid (PCA) in murine OS cells (UMR-106). The concentrations were 3.12 μM of isolated MEN, 500 μM of isolated PCA, and their associations. We performed cell viability assays, morphology modification analysis, cell migration by the wound-healing method, apoptosis by flow cytometry, reactive oxygen species (ROS) production, gene expression of NOX by RT-qPCR, and degradation of MMP-2 and 9 by zymography. Our results showed that the association of MEN+PCA was more effective in OS cells than the compounds alo...
Source: Archives of Biochemistry and Biophysics - December 1, 2023 Category: Biochemistry Authors: Kelly Karina Inacio Adriano de Souza Pessoa Cintia Kazuko Tokuhara Ana L ígia Pagnan Mariana Liessa Rovis Sanches Vanessa Svizzero Fakhoury Gabriela Silva Neubern de Oliveira Flavia Amadeu de Oliveira Valdecir Farias Ximenes Rodrigo Cardoso de Oliveira Source Type: research

Molecular characterization of STEAP3 in lung squamous cell carcinoma: Regulating EGFR to affect cell proliferation and ferroptosis
Arch Biochem Biophys. 2023 Nov 29:109842. doi: 10.1016/j.abb.2023.109842. Online ahead of print.ABSTRACTSix-transmembrane epithelial antigen of the prostate 3 (STEAP3) has been reported to play a regulatory role in various types of cancers. However, its involvement in lung squamous cell carcinoma (LUSC) remains understudied. Here, we aimed to explore the biological functions and underlying mechanisms of STEAP3 in LUSC. Intersection genes associated with LUSC and ferroptosis were analyzed using the Venn method, STRING, GEPIA and UALCAN databases. The expression of STEAP3 was detected by qPCR and western blotting assay. Cell...
Source: Archives of Biochemistry and Biophysics - December 1, 2023 Category: Biochemistry Authors: Hanqiang Shi Siyu Lei Lie Xiong Shuqin Du Yanbo Shi Source Type: research

M2 macrophage-derived exosomes induce angiogenesis and increase skin flap survival through HIF1AN/HIF-1 α/VEGFA control
CONCLUSION: This study showed that M2-exosomes promote skin flap survival by enhancing angiogenesis, with HIF1AN/HIF-1α/VEGFA playing a crucial role in this process.PMID:38030054 | DOI:10.1016/j.abb.2023.109822 (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - November 29, 2023 Category: Biochemistry Authors: Gaojie Luo Zekun Zhou Zheming Cao Chengxiong Huang Cheng Li Xiaoxiao Li Chao Deng Panfeng Wu Zhenni Yang Juyu Tang Liming Qing Source Type: research

A novel highly thermostable and stress resistant ROS scavenging metalloprotein from Paenibacillus
Arch Biochem Biophys. 2023 Nov 23;751:109837. doi: 10.1016/j.abb.2023.109837. Online ahead of print.ABSTRACTReactive oxygen species (ROS) are unstable metabolites produced during cellular respiration that can cause extensive damage to the body. Here we report a unique structural metalloprotein called RSAPp for the first time, which exhibits robust ROS-scavenging activity, high thermostability, and stress resistance. RSAPp is a previously uncharacterized DUF2935 (domain of unknown function, accession number: cl12705) family protein from Paenibacillus, containing a highly conserved four-helix bundle with binding sites for va...
Source: Archives of Biochemistry and Biophysics - November 25, 2023 Category: Biochemistry Authors: Jingjing Zhang Jiabin Li Jingbo Gong Jingjing Liu Yijia Wang Fang Zhao Shenmei Sun Wei Wang Source Type: research

A novel highly thermostable and stress resistant ROS scavenging metalloprotein from Paenibacillus
Arch Biochem Biophys. 2023 Nov 23:109837. doi: 10.1016/j.abb.2023.109837. Online ahead of print.ABSTRACTReactive oxygen species (ROS) are unstable metabolites produced during cellular respiration that can cause extensive damage to the body. Here we report a unique structural metalloprotein called RSAPp for the first time, which exhibits robust ROS-scavenging activity, high thermostability, and stress resistance. RSAPp is a previously uncharacterized DUF2935 (domain of unknown function, accession number: cl12705) family protein from Paenibacillus, containing a highly conserved four-helix bundle with binding sites for variab...
Source: Archives of Biochemistry and Biophysics - November 25, 2023 Category: Biochemistry Authors: Jingjing Zhang Jiabin Li Jingbo Gong Jingjing Liu Yijia Wang Fang Zhao Shenmei Sun Wei Wang Source Type: research

In silico study of the impact of oxidation on pyruvate transmission across the hVDAC1 protein channel
In this study, we used molecular dynamics (MD) simulations to investigate the effect of a low level of VDAC1 oxidation (induced e.g., by cold atmospheric plasma (CAP)) on the pyruvate (Pyr) uptake by VDAC1. Inhibiting Pyr uptake through VDAC1 can suppress cancer cell proliferation. Our primary target was to study the translocation of Pyr across the native and oxidized forms of hVDAC1, the human VDAC1. Specifically, we employed MD simulations to analyze the hVDAC1 structure by modifying certain cysteine residues to cysteic acids and methionine residues to methionine sulfoxides, which allowed us to investigate the effect of ...
Source: Archives of Biochemistry and Biophysics - November 24, 2023 Category: Biochemistry Authors: Mahsa Rezaei Maryam Ghasemitarei Jamoliddin Razzokov Maksudbek Yusupov Mohammad Ghorbanalilu Mohammad Reza Ejtehadi Source Type: research

The interaction of the ribotoxin α-sarcin with complex model lipid vesicles
Arch Biochem Biophys. 2023 Nov 22:109836. doi: 10.1016/j.abb.2023.109836. Online ahead of print.ABSTRACTFungal ribotoxins are extracellular RNases that inactivate ribosomes by cleaving a single phosphodiester bond at the universally conserved sarcin-ricin loop of the large rRNA. However, to reach the ribosomes, they need to cross the plasma membrane. It is there where these toxins show their cellular specificity, being especially active against tumoral or virus-infected cells. Previous studies have shown that fungal ribotoxins interact with negatively charged membranes, typically containing phosphatidylserine or phosphatid...
Source: Archives of Biochemistry and Biophysics - November 24, 2023 Category: Biochemistry Authors: Carmen Garc ía-Montoya Sara Garc ía-Linares Diego Heras-M árquez Manca Majnik Diego Laxalde-Fern ández Rafael Amigot-S ánchez Álvaro Martínez-Del-Pozo Juan Palacios-Ortega Source Type: research