tRNA-derived fragments: Key determinants of cancer metastasis with emerging therapeutic and diagnostic potentials
Arch Biochem Biophys. 2024 Feb 16:109930. doi: 10.1016/j.abb.2024.109930. Online ahead of print.ABSTRACTMetastasis is a significant clinical challenge responsible for cancer mortality and non-response to treatment. However, the molecular mechanisms driving metastasis remain unclear, limiting the development of efficient diagnostic and therapeutic approaches. Recent breakthroughs in cancer biology have discovered a group of small non-coding RNAs called tRNA-derived fragments (tRFs), which play a critical role in the metastatic behavior of various tumors. tRFs are produced from cleavage modifications of tRNAs and have differ...
Source: Archives of Biochemistry and Biophysics - February 18, 2024 Category: Biochemistry Authors: Mohammad Salehi Mohammad Javad Kamali Aliakbar Rajabzadeh Shima Minoo Hamidreza Mosharafi Fatemeh Saeedi Abdolreza Daraei Source Type: research

Dihydrocelastrol induces cell death and suppresses angiogenesis through BCR/AP-1/junb signalling in diffuse large B cell lymphoma
Arch Biochem Biophys. 2024 Feb 15:109929. doi: 10.1016/j.abb.2024.109929. Online ahead of print.ABSTRACTDiffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma. Although treatment options have improved, a large proportion of patients show low survival rates, highlighting an urgent need for novel therapeutic strategies. The aim of this study was to investigate the efficacy of the new small-molecule compound dihydrocelastrol (DHCE), acquired through the structural modification of celastrol (CE), in the treatment of DLBCL. DHCE showed potent anti-lymphoma efficacy and synergistic effects with dox...
Source: Archives of Biochemistry and Biophysics - February 17, 2024 Category: Biochemistry Authors: Yue Lai Shushan Guo Qiongwei Tang Gaomei Chang Hui Zhang Bo Li Qilin Feng Ke Hu Zhijian Xu Xuejie Gao Qikai Zhang Hongfei Yi Dongliang Song Yifei Zhang Yu Peng Haiyan Cai Weiliang Zhu Jumei Shi Source Type: research

Involvement of the Keap1-Nrf2-ARE pathway in the antioxidant activity of sinomenine
This study is aimed to investigate the critical role of the nuclear factor erythroid 2-related factor 2 (Nrf2)-kelch-like ECH-associated protein-1(Keap1)-antioxidant response element (ARE) antioxidative signaling pathway in protecting sinomenine against H2O2-induced oxidative injury. Cytotoxicity and antioxidant experiments to initially determine the protective effects of sinomenine show that sinomenine has no effect on the decreased cell viability and presents similar potency in scavenging all three free radicals. The binding affinity between sinomenine and Keap1 was determined via fluorescence polarization assay, with IC...
Source: Archives of Biochemistry and Biophysics - February 14, 2024 Category: Biochemistry Authors: Tianzhu Guan Ning Li Xiangxiu Xu Dan Xiong Bo Wang Lixia Xiao Weikang Yang Ganghui Chu Abdulla Yusuf Jie Zhang Weiming Yue Source Type: research

Interrogating l-fuconate dehydratase with tartronate and 3-hydroxypyruvate reveals subtle differences within the mandelate racemase-subgroup of the enolase superfamily
We report a circular dichroism-based assay of FucD activity that monitors the change in ellipticity at 216 nm (Δ[Θ]S-P = 8985 ± 87 deg cm2 mol-1) accompanying the conversion of l-fuconate to 2-keto-3-deoxy-l-fuconate. Tartronate was a linear mixed-type inhibitor of FucD (Ki = 8.4 ± 0.7 mM, αKi = 63 ± 11 mM), binding 18-fold weaker than l-fuconate, compared with 2-fold weaker binding of tartronate by MR relative to mandelate. 3-HP irreversibly inactivated FucD (kinact/KI = 0.018 ± 0.002 M-1s-1) with an efficiency that was ∼4.6 × 102-fold less than that observed with MR. The inactivation arose predominantly from mo...
Source: Archives of Biochemistry and Biophysics - February 14, 2024 Category: Biochemistry Authors: Laura C McGary Christopher M Fetter Minglu Gu Meghan C Hamilton Himank Kumar Oliver P Kuehm Colin D Douglas Stephen L Bearne Source Type: research

Involvement of the Keap1-Nrf2-ARE pathway in the antioxidant activity of sinomenine
This study is aimed to investigate the critical role of the nuclear factor erythroid 2-related factor 2 (Nrf2)-kelch-like ECH-associated protein-1(Keap1)-antioxidant response element (ARE) antioxidative signaling pathway in protecting sinomenine against H2O2-induced oxidative injury. Cytotoxicity and antioxidant experiments to initially determine the protective effects of sinomenine show that sinomenine has no effect on the decreased cell viability and presents similar potency in scavenging all three free radicals. The binding affinity between sinomenine and Keap1 was determined via fluorescence polarization assay, with IC...
Source: Archives of Biochemistry and Biophysics - February 14, 2024 Category: Biochemistry Authors: Tianzhu Guan Ning Li Xiangxiu Xu Dan Xiong Bo Wang Lixia Xiao Weikang Yang Ganghui Chu Abdulla Yusuf Jie Zhang Weiming Yue Source Type: research

Interrogating l-fuconate dehydratase with tartronate and 3-hydroxypyruvate reveals subtle differences within the mandelate racemase-subgroup of the enolase superfamily
We report a circular dichroism-based assay of FucD activity that monitors the change in ellipticity at 216 nm (Δ[Θ]S-P = 8985 ± 87 deg cm2 mol-1) accompanying the conversion of l-fuconate to 2-keto-3-deoxy-l-fuconate. Tartronate was a linear mixed-type inhibitor of FucD (Ki = 8.4 ± 0.7 mM, αKi = 63 ± 11 mM), binding 18-fold weaker than l-fuconate, compared with 2-fold weaker binding of tartronate by MR relative to mandelate. 3-HP irreversibly inactivated FucD (kinact/KI = 0.018 ± 0.002 M-1s-1) with an efficiency that was ∼4.6 × 102-fold less than that observed with MR. The inactivation arose predominantly from mo...
Source: Archives of Biochemistry and Biophysics - February 14, 2024 Category: Biochemistry Authors: Laura C McGary Christopher M Fetter Minglu Gu Meghan C Hamilton Himank Kumar Oliver P Kuehm Colin D Douglas Stephen L Bearne Source Type: research

Involvement of the Keap1-Nrf2-ARE pathway in the antioxidant activity of sinomenine
This study is aimed to investigate the critical role of the nuclear factor erythroid 2-related factor 2 (Nrf2)-kelch-like ECH-associated protein-1(Keap1)-antioxidant response element (ARE) antioxidative signaling pathway in protecting sinomenine against H2O2-induced oxidative injury. Cytotoxicity and antioxidant experiments to initially determine the protective effects of sinomenine show that sinomenine has no effect on the decreased cell viability and presents similar potency in scavenging all three free radicals. The binding affinity between sinomenine and Keap1 was determined via fluorescence polarization assay, with IC...
Source: Archives of Biochemistry and Biophysics - February 14, 2024 Category: Biochemistry Authors: Tianzhu Guan Ning Li Xiangxiu Xu Dan Xiong Bo Wang Lixia Xiao Weikang Yang Ganghui Chu Abdulla Yusuf Jie Zhang Weiming Yue Source Type: research

Interrogating l-fuconate dehydratase with tartronate and 3-hydroxypyruvate reveals subtle differences within the mandelate racemase-subgroup of the enolase superfamily
We report a circular dichroism-based assay of FucD activity that monitors the change in ellipticity at 216 nm (Δ[Θ]S-P = 8985 ± 87 deg cm2 mol-1) accompanying the conversion of l-fuconate to 2-keto-3-deoxy-l-fuconate. Tartronate was a linear mixed-type inhibitor of FucD (Ki = 8.4 ± 0.7 mM, αKi = 63 ± 11 mM), binding 18-fold weaker than l-fuconate, compared with 2-fold weaker binding of tartronate by MR relative to mandelate. 3-HP irreversibly inactivated FucD (kinact/KI = 0.018 ± 0.002 M-1s-1) with an efficiency that was ∼4.6 × 102-fold less than that observed with MR. The inactivation arose predominantly from mo...
Source: Archives of Biochemistry and Biophysics - February 14, 2024 Category: Biochemistry Authors: Laura C McGary Christopher M Fetter Minglu Gu Meghan C Hamilton Himank Kumar Oliver P Kuehm Colin D Douglas Stephen L Bearne Source Type: research

Hyperglycemia - A culprit of podocyte pathology in the context of glycogen metabolism
Arch Biochem Biophys. 2024 Feb 11:109927. doi: 10.1016/j.abb.2024.109927. Online ahead of print.ABSTRACTProlonged disruption in the balance of glucose can result in metabolic disorders. The kidneys play a significant role in regulating blood glucose levels. However, when exposed to chronic hyperglycemia, the kidneys' ability to handle glucose metabolism may be impaired, leading to an accumulation of glycogen. Earlier studies have shown that there can be a significant increase in glucose storage in the form of glycogen in the kidneys in diabetes. Podocytes play a crucial role in maintaining the integrity of filtration barri...
Source: Archives of Biochemistry and Biophysics - February 13, 2024 Category: Biochemistry Authors: Olga Żołnierkiewicz Dorota Rogacka Source Type: research

Molecular basis of ligand recognition specificity of flavone glucosyltransferases in Nemophila menziesii
Arch Biochem Biophys. 2024 Feb 10:109926. doi: 10.1016/j.abb.2024.109926. Online ahead of print.ABSTRACTOf the more than 100 families of glycosyltransferases, family 1 glycosyltransferases catalyze glycosylation using uridine diphosphate (UDP)-sugar as a sugar donor and are thus referred to as UDP-sugar:glycosyl transferases. The blue color of the Nemophila menziesii flower is derived from metalloanthocyanin, which consists of anthocyanin, flavone, and metal ions. Flavone 7-O-β-glucoside-4'-O-β-glucoside in the plant is sequentially biosynthesized from flavons by UDP-glucose:flavone 4'-O-glucosyltransferase (NmF4'GT) and...
Source: Archives of Biochemistry and Biophysics - February 12, 2024 Category: Biochemistry Authors: Kazutaka Murayama Miyuki Kato-Murayama Toshiaki Hosaka Naoko Okitsu Yoshikazu Tanaka Mikako Shirouzu Source Type: research

YAP1-activated ZNF131 promotes hepatocellular carcinoma cell proliferation through transcriptional regulation of PAIP1
Arch Biochem Biophys. 2024 Feb 8:109921. doi: 10.1016/j.abb.2024.109921. Online ahead of print.ABSTRACTZinc finger protein 131 (ZNF131), a member of BTB-ZF transcription factors, has been previously reported as an oncogene in several human cancers. However, the function and underlying mechanism of ZNF131 in hepatocellular carcinoma (HCC) are still unclear. In our study, the upregulated expression of ZNF131 mRNA was confirmed in HCC tissues by analyzing the TCGA and GEO datasets. The immunohistochemical staining data also revealed the overexpression of ZNF131 protein in HCC samples. High expression of ZNF131 predicted poor ...
Source: Archives of Biochemistry and Biophysics - February 10, 2024 Category: Biochemistry Authors: Guozhi Yin Siying Jia Youju Zhang Yao Xian Yong Guo Qingguang Liu Source Type: research

Cinnamaldehyde attenuates TNF- α induced skeletal muscle loss in C2C12 myotubes via regulation of protein synthesis, proteolysis, oxidative stress and inflammation
Arch Biochem Biophys. 2024 Feb 8:109922. doi: 10.1016/j.abb.2024.109922. Online ahead of print.ABSTRACTInflammation is the primary driver of skeletal muscle wasting, with oxidative stress serving as both a major consequence and a contributor to its deleterious effects. In this regard, regulation of both can efficiently prevent atrophy and thus will increase the rate of survival (Meng and Yu, 2010) [1]. With this idea, we hypothesize that preincubation of Cinnamaldehyde (CNA), a known compound with anti-oxidative and anti-inflammatory properties, may be able to prevent skeletal muscle loss. To examine the same, C2C12 post-d...
Source: Archives of Biochemistry and Biophysics - February 10, 2024 Category: Biochemistry Authors: Nirmaljeet Kaur Prachi Gupta Vikas Dutt Onkar Sharma Sanjeev Gupta Anita Dua Elisha Injeti Ashwani Mittal Source Type: research

TRIM34 suppresses non-small-cell lung carcinoma via inducing mTORC1-dependent glucose utilization and promoting cellular death
This study aimed to examine the underlying regulatory effects of TRIM34 in NSCLC. TRIM34 exhibited lower expression in NSCLC. TRIM34 facilitated mitochondrial damage and apoptosis in NSCLC. TRIM34 induced the increased activity of mTORC1 and accelerated glycolysis in NSCLC. Enhanced mitochondrial damage induced by TRIM34 overexpression was reversed after rapamycin (mTORC1 inhibitor) treatment in NSCLC. The strengthened cell apoptosis stimulated by TRIM34 overexpression was rescued after rapamycin treatment. TRIM34 activated mTORC1 to suppress NSCLC progression in vivo. TRIM34 suppressed NSCLC via inducing mTORC1-dependent ...
Source: Archives of Biochemistry and Biophysics - February 9, 2024 Category: Biochemistry Authors: Pengfei Zhang Zhida Chen Juan Li Hui Mao Yi Hu Source Type: research

PTEN hinders the formation of scars by regulating the levels of proteins in the extracellular matrix and promoting the apoptosis of dermal fibroblasts through Bcl-xL
The objective of this study was to investigate the influence of PTEN on hypertrophic scars(HS) and its function in the regulation of scar formation, with the aim of identifying a pivotal molecular target for scar treatment. Our results demonstrate that the overexpression of PTEN (AdPTEN) significantly suppressed the expression of type I collagen (Col I), type III collagen (Col III), and alpha smooth muscle actin (α-SMA) in HSFs. Furthermore, it was observed that the introduction of AdPTEN resulted in the suppression of Bcl-xL expression, which consequently led to an increase in the apoptosis of HSFs. Similarly, in the inh...
Source: Archives of Biochemistry and Biophysics - February 7, 2024 Category: Biochemistry Authors: Shaohui Li Yunwei Wang Yang Chen Hao Zhang Peng Cao Kuo Shen Hao Guan Source Type: research

GDNF facilitates the differentiation of ADSCs to Schwann cells and enhances nerve regeneration through GDNF/MTA1/Hes1 axis
Arch Biochem Biophys. 2024 Feb 1;753:109893. doi: 10.1016/j.abb.2024.109893. Online ahead of print.ABSTRACTAdipose tissue-derived stem cells (ADSCs) are a kind of stem cells with multi-directional differentiation potential, which mainly restore tissue repair function and promote cell regeneration. It can be directionally differentiated into Schwann-like cells to promote the repair of peripheral nerve injury. Glial cell line-derived neurotrophic factor (GDNF) plays an important role in the repair of nerve injury, but the underlying mechanism remains unclear, which seriously limits its further application.The study aimed to ...
Source: Archives of Biochemistry and Biophysics - February 3, 2024 Category: Biochemistry Authors: Weixia Cai Yang Liu Ting Zhang Peng Ji Chenyang Tian Jiaqi Liu Zhao Zheng Source Type: research