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ACE2 activation alleviates sepsis-induced cardiomyopathy by promoting MasR-Sirt1-mediated mitochondrial biogenesis
Arch Biochem Biophys. 2023 Dec 12:109855. doi: 10.1016/j.abb.2023.109855. Online ahead of print.ABSTRACTSepsis-induced cardiomyopathy (SIC), caused by a dysregulated host response to infection, is a major contributor to high mortality. Angiotensin-converting enzyme 2 (ACE2), a crucial component of the renin-angiotensin system (RAS), has protective effects against several cardiovascular diseases, such as myocardial infarction and heart failure. However, the role of ACE2 in the pathogenesis of SIC and underlying mechanisms remain unknown. The present study was designed to examine the effects of ACE2 activation or inhibition ...
Source: Archives of Biochemistry and Biophysics - December 14, 2023 Category: Biochemistry Authors: Tian-Tian Wan Ya Li Jia-Xin Li Xue Xiao Lei Liu Hui-Hua Li Shu-Bin Guo Source Type: research

Recent advances in de novo computational design and redesign of intrinsically disordered proteins and intrinsically disordered protein regions
Arch Biochem Biophys. 2023 Dec 13;752:109857. doi: 10.1016/j.abb.2023.109857. Online ahead of print.ABSTRACTIn the early 2000s, the concept of "unstructured biology" has emerged to be an important field in protein science by generating various new research directions. Many novel strategies and methods have been developed that are focused on effectively identifying/predicting intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs), identifying their potential functions, disorder based drug design etc. Due to the range of functions of IDPs/IDPRs and their involvement in various debilitat...
Source: Archives of Biochemistry and Biophysics - December 14, 2023 Category: Biochemistry Authors: Bondeepa Saikia Anupaul Baruah Source Type: research

Recent advances in de novo computational design and redesign of intrinsically disordered proteins and intrinsically disordered protein regions
Arch Biochem Biophys. 2023 Dec 12:109857. doi: 10.1016/j.abb.2023.109857. Online ahead of print.ABSTRACTIn the early 2000s, the concept of "unstructured biology" has emerged to be an important field in protein science by generating various new research directions. Many novel strategies and methods have been developed that are focused on effectively identifying/predicting intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs), identifying their potential functions, disorder based drug design etc. Due to the range of functions of IDPs/IDPRs and their involvement in various debilitating ...
Source: Archives of Biochemistry and Biophysics - December 14, 2023 Category: Biochemistry Authors: Bondeepa Saikia Anupaul Baruah Source Type: research

4-octyl itaconate ameliorates ventilator-induced lung injury
In this study, we assessed whether OI protects against VILI. OI was intraperitoneally injected for three days before mechanical ventilation (MV; 20 ml/kg at 70 breaths/min) for 2 h. Mouse lung vascular endothelial cells (MLVECs) were pretreated with OI (62.5, 125, and 250 μM) prior to cyclic stretch for 4 h. We found that OI attenuated VILI and inflammatory response. OI also increased superoxide dismutase, nuclear factor E2-related factor 2, and heme oxygenase-1 levels, and decreased reactive oxygen species and malondialdehyde levels. Furthermore, OI inhibited the expression of NLR family pyrin domain-containing 3 (NLRP3)...
Source: Archives of Biochemistry and Biophysics - December 12, 2023 Category: Biochemistry Authors: Xiudan Wang Weijing Kong Rui Yang Chunyan Yang Source Type: research

Product inhibition slow down the moving velocity of processive chitinase and sliding-intermediate state blocks re-binding of product
Arch Biochem Biophys. 2023 Dec 9:109854. doi: 10.1016/j.abb.2023.109854. Online ahead of print.ABSTRACTProcessive movement is the key reaction for crystalline polymer degradation by enzyme. Product release is an important phenomenon in resetting the moving cycle, but how it affects chitinase kinetics was unknown. Therefore, we investigated the effect of diacetyl chitobiose (C2) on the biochemical activity and movement of chitinase A from Serratia marcescens (SmChiA). The apparent inhibition constant of C2 on crystalline chitin degradation of SmChiA was 159 μM. The binding position of C2 obtained by X-ray crystallography w...
Source: Archives of Biochemistry and Biophysics - December 11, 2023 Category: Biochemistry Authors: Yoshiko Tanaka Takayuki Uchihashi Akihiko Nakamura Source Type: research

ICOSLG acts as an oncogene to promote glycolysis, proliferation, migration, and invasion in gastric cancer cells
This study aims to investigate the role of ICOSLG and its potential mechanism of action in dictating the aggressiveness of GC cell. ICOSLG and miR-331-3p expression patterns in cancerous and para-cancerous tissues from GC patients were examined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The miRNAs targeting ICOSLG were predicted by "miRDB", "starBase," and "TargetScan" databases. The interplay of ICOSLG and miR-331-3p in dictating the aggressiveness and glycolysis of GC cells was investigated by CCK-8 proliferation assay and Transwell migration/invasion assays, as well as the detection of gl...
Source: Archives of Biochemistry and Biophysics - December 11, 2023 Category: Biochemistry Authors: Li Zhang Yunge Gao Source Type: research

CYP108N14: A Monoterpene Monooxygenase from Rhodococcus globerulus
Arch Biochem Biophys. 2023 Dec 8:109852. doi: 10.1016/j.abb.2023.109852. Online ahead of print.ABSTRACTRhodococcus globerulus (R. globerulus) was isolated from the soil beneath a Eucalypt tree. Metabolic growth studies revealed that R. globerulus was capable of living on certain monoterpenes, including 1,8-cineole and p-cymene, as sole sources of carbon and energy. Multiple P450 genes were identified in the R. globerulus genome that shared homology to known bacterial, monoterpene hydroxylating P450s. To date, two of these P450s have been expressed and characterised as 1,8-cineole (CYP176A1) and p-cymene (CYP108N12) monooxy...
Source: Archives of Biochemistry and Biophysics - December 10, 2023 Category: Biochemistry Authors: Peter D Giang Luke R Churchman Julia B Buczynski Stephen G Bell Jeanette E Stok James J De Voss Source Type: research

Self-assembling amyloid-like nanostructures from SARS-CoV-2 S1, S2, RBD and N recombinant proteins
Arch Biochem Biophys. 2023 Dec 8:109843. doi: 10.1016/j.abb.2023.109843. Online ahead of print.ABSTRACTSelf-assembling nanoparticles (saNP) and nanofibers were found in the recombinant coronavirus SARS-CoV-2 S1, S2, RBD and N proteins purified by affinity chromatography using Ni Sepharose. Scanning electron (SEM), atomic force (AFM) microscopy on mica or graphite surface and in liquid as well as dynamic light scattering (DLS) revealed nanostructures of various sizes. AFM in liquid cell without drying on the surface showed mean sizes of S1 saNP 80.03 nm, polydispersity index (PDI) 0.078; for S2 saNP mean size 93.32 nm, PDI ...
Source: Archives of Biochemistry and Biophysics - December 10, 2023 Category: Biochemistry Authors: Olga V Morozova Valentin A Manuvera Nikolay A Barinov Elena N Subcheva Victor S Laktyushkin Dimitri A Ivanov Vassili N Lazarev Dmitry V Klinov Source Type: research