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Echinacoside stimulates myogenesis and ATP-dependent thermogenesis in the skeletal muscle via the activation of D1-like dopaminergic receptors
In this study, we investigated the effect of echinacoside (ECH), a caffeic acid glycoside from the phenylpropanoid class, on myogenesis and ATP-dependent thermogenesis in the skeletal muscle and its interaction with the dopaminergic receptors 1 and 5 (DRD1 and DRD5). We applied RT-PCR, immunoblot analysis, a staining method, and an assay kit to determine the effects of ECH on diverse target genes and proteins involved in skeletal muscle myogenesis and ATP-consuming futile processes. Our study demonstrated that ECH enhanced myogenic differentiation, glucose, and fatty acid uptake, as well as lipid catabolism, and induced AT...
Source: Archives of Biochemistry and Biophysics - January 12, 2024 Category: Biochemistry Authors: Kiros Haddish Jong Won Yun Source Type: research

The presence of free palmitic acid modulates the effects of lutein on structural and dynamic properties of lipid membranes
We presented the simplified model membrane that could be a suitable representation of the physiological process of de-esterification of PA from LUT appearing in natural biomembranes in humans.PMID:38211638 | DOI:10.1016/j.abb.2024.109883 (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - January 11, 2024 Category: Biochemistry Authors: Emilia Reszczy ńska Anna Wi śniewska-Becker Mariusz Duda Alicja S ęk Wies ław I Gruszecki Agnieszka Hanaka Source Type: research

G protein-coupled estrogen receptor (GPER)/GPR30 forms a complex with the β < sub > 1 < /sub > -adrenergic receptor, a membrane-associated guanylate kinase (MAGUK) scaffold protein, and protein kinase A anchoring protein (AKAP) 5 in MCF7 breast cancer cells
Arch Biochem Biophys. 2024 Feb;752:109882. doi: 10.1016/j.abb.2024.109882. Epub 2024 Jan 10.ABSTRACTG protein-coupled receptor 30 (GPR30), also named G protein-coupled estrogen receptor (GPER), and the β1-adrenergic receptor (β1AR) are G protein-coupled receptors (GPCR) that are implicated in breast cancer progression. Both receptors contain PSD-95/Discs-large/ZO-1 homology (PDZ) motifs in their C-terminal tails through which they interact in the plasma membrane with membrane-associated guanylate kinase (MAGUK) scaffold proteins, and in turn protein kinase A anchoring protein (AKAP) 5. GPR30 constitutively and PDZ-depend...
Source: Archives of Biochemistry and Biophysics - January 11, 2024 Category: Biochemistry Authors: Julia Tutzauer D Stephen Serafin Tobias Schmidt Bj örn Olde Kathleen M Caron L M Fredrik Leeb-Lundberg Source Type: research

Myopathy-causing mutation R91P in the TPM3 gene drastically impairs structural and functional properties of slow skeletal muscle tropomyosin γβ-heterodimer
Arch Biochem Biophys. 2024 Feb;752:109881. doi: 10.1016/j.abb.2023.109881. Epub 2024 Jan 6.ABSTRACTTropomyosin (Tpm) is a regulatory actin-binding protein involved in Ca2+ activation of contraction of striated muscle. In human slow skeletal muscles, two distinct Tpm isoforms, γ and β, are present. They interact to form three types of dimeric Tpm molecules: γγ-homodimers, γβ-heterodimers, or ββ-homodimers, and a majority of the molecules are present as γβ-Tpm heterodimers. Point mutation R91P within the TPM3 gene encoding γ-Tpm is linked to the condition known as congenital fiber-type disproportion (CFTD), which ...
Source: Archives of Biochemistry and Biophysics - January 7, 2024 Category: Biochemistry Authors: Anastasiia D Gonchar Natalia A Koubassova Galina V Kopylova Anastasia M Kochurova Victoria V Nefedova Daria S Yampolskaya Daniil V Shchepkin Sergey Y Bershitsky Andrey K Tsaturyan Alexander M Matyushenko Dmitrii I Levitsky Source Type: research

Myopathy-causing mutation R91P in the TPM3 gene drastically impairs structural and functional properties of slow skeletal muscle tropomyosin γβ-heterodimer
Arch Biochem Biophys. 2024 Jan 5:109881. doi: 10.1016/j.abb.2023.109881. Online ahead of print.ABSTRACTTropomyosin (Tpm) is a regulatory actin-binding protein involved in Ca2+ activation of contraction of striated muscle. In human slow skeletal muscles, two distinct Tpm isoforms, γ and β, are present. They interact to form three types of dimeric Tpm molecules: γγ-homodimers, γβ-heterodimers, or ββ-homodimers, and a majority of the molecules are present as γβ-Tpm heterodimers. Point mutation R91P within the TPM3 gene encoding γ-Tpm is linked to the condition known as congenital fiber-type disproportion (CFTD), wh...
Source: Archives of Biochemistry and Biophysics - January 7, 2024 Category: Biochemistry Authors: Anastasiia D Gonchar Natalia A Koubassova Galina V Kopylova Anastasia M Kochurova Victoria V Nefedova Daria S Yampolskaya Daniil V Shchepkin Sergey Y Bershitsky Andrey K Tsaturyan Alexander M Matyushenko Dmitrii I Levitsky Source Type: research

Middle-age abolishes cardioprotection conferred by thioredoxin-1 in mice
This study aimed to evaluate if combining two cardioprotective strategies, such as Trx1 overexpression and PostC, could exert a synergistic effect in reducing infarct size in middle-aged mice. Young or middle-aged wild-type mice (Wt), transgenic mice overexpressing Trx1, and dominant negative (DN-Trx1) mutant of Trx1 mice were used. Mice hearts were subjected to I/R or PostC protocol. Infarct size, hydrogen peroxide (H2O2) production, protein nitration, Trx1 activity, mitochondrial function, and Trx1, pAkt and pGSK3β expression were measured. PostC could not reduce infarct size even in the presence of Trx1 overexpression ...
Source: Archives of Biochemistry and Biophysics - January 3, 2024 Category: Biochemistry Authors: V Perez T Zaobornyj T Vico V Vanasco T Marchini E Godoy S Alvarez P Evelson M Donato R J Gelpi V D'Annunzio Source Type: research

Characterization and anti-tumor activities of polysaccharide isolated from Brassica rapa L. via activation of macrophages through TLR2-and TLR4-Dependent pathways
In this report, we determined the characteristics of Nozawana polysaccharide (NPS) and evaluated the immunomodulatory effects and anti-tumor activity of NPS mediated by macrophage activation. The molecular weight of NPS was determined by gel filtration chromatography with an average molecular weight of approximately 100.6 kDa. HPLC analysis showed that NPS contained glucose, galacturonic acid, galactose, and arabinose. NPS increased cytokine and nitric oxide (NO) production by macrophages in a Toll-like receptor (TLR)2 and TLR4-dependent manner. Furthermore, NPS induced apoptosis significantly against 4T1 murine breast can...
Source: Archives of Biochemistry and Biophysics - December 31, 2023 Category: Biochemistry Authors: Rina Matsui Katsunori Endo Takeru Saiki Hazuki Haga Weidong Shen Xiangdong Wang Shinya Yamazaki Shigeru Katayama Kenji Nagata Hidemitsu Kitamura Sachi Tanaka Source Type: research

Evidence for bidirectional formic acid translocation in vivo via the Escherichia coli formate channel FocA
Arch Biochem Biophys. 2023 Dec 28:109877. doi: 10.1016/j.abb.2023.109877. Online ahead of print.ABSTRACTPentameric FocA permeates either formate or formic acid bidirectionally across the cytoplasmic membrane of anaerobically growing Escherichia coli. Each protomer of FocA has its own hydrophobic pore, but it is unclear whether formate or neutral formic acid is translocated in vivo. Here, we measured total and dicyclohexylcarbodiimide (DCCD)-inhibited proton flux out of resting, fermentatively grown, stationary-phase E. coli cells in dependence on FocA. Using a wild-type strain synthesizing native FocA, it was shown that us...
Source: Archives of Biochemistry and Biophysics - December 30, 2023 Category: Biochemistry Authors: Liana Vanyan Michelle Kammel R Gary Sawers Karen Trchounian Source Type: research

Tunnel-like region observed as a potential allosteric site in Staphylococcus aureus Glyceraldehyde-3-phosphate dehydrogenase
Arch Biochem Biophys. 2023 Dec 28;752:109875. doi: 10.1016/j.abb.2023.109875. Online ahead of print.ABSTRACTGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzing the sixth step of glycolysis has been investigated for allosteric features that might be used as potential target for specific inhibition of Staphylococcus aureus (S.aureus). X-ray structure of bacterial enzyme for which a tunnel-like opening passing through the center previously proposed as an allosteric site has been subjected to six independent 500 ns long Molecular Dynamics simulations. Harmonic bond restraints were employed at key residues to underline ...
Source: Archives of Biochemistry and Biophysics - December 29, 2023 Category: Biochemistry Authors: Ozde Zeynep Guner-Y ılmaz Ozge Kurkcuoglu Ebru Demet Akten Source Type: research

Mechanism by which HDAC3 regulates manganese induced H3K27ac in SH-SY5Y cells and intervention by curcumin
In this study, SH-SY5Y cells were exposed to manganese for 24 h, were treated with TSA or ITSA-1, and were treated with or without Cur. The results suggested that manganese exposure, which leads to increased expression of HDAC3, induced H3K27 hypoacetylation, inhibited the transcription of antioxidant genes, decreased antioxidant enzyme activities, and induced oxidative damage in cells. Pretreatment with an HDAC3 inhibitor (TSA) increased the acetylation of H3K27 and the transcription of antioxidant genes and thus slowed manganese exposure-induced cellular oxidative damage. In contrast, an HDAC3 activator (ITSA-1) partiall...
Source: Archives of Biochemistry and Biophysics - December 27, 2023 Category: Biochemistry Authors: Ying Liu Hua Zhao Yue Yang Yan Liu Chun-Yan Ao Jia-Min Zeng Jia-Qi Ban Jun Li Source Type: research