Applied Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Glucosylglycerol phosphorylase, a potential novel pathway of microbial glucosylglycerol catabolism
Appl Microbiol Biotechnol. 2024 Feb 16;108(1):214. doi: 10.1007/s00253-024-13035-3.ABSTRACTGlucosylglycerol (GG) is a natural compatible solute that can be synthesized by many cyanobacteria and a few heterotrophic bacteria under high salinity conditions. In cyanobacteria, GG is synthesized by GG-phosphate synthase and GG-phosphate phosphatase, and a hydrolase GGHA catalyzes its degradation. In heterotrophic bacteria (such as some Marinobacter species), a fused form of GG-phosphate phosphatase and GG-phosphate synthase is present, but the cyanobacteria-like degradation pathway is not available. Instead, a phosphorylase GGP,...
Source: Applied Microbiology and Biotechnology - February 16, 2024 Category: Microbiology Authors: Lin Cheng Zhichao Zhang Daling Zhu Quan Luo Xuefeng Lu Source Type: research

Xylanase treatment of eucalypt kraft pulps: effect of carryover
Appl Microbiol Biotechnol. 2024 Feb 15;108(1):210. doi: 10.1007/s00253-024-13027-3.ABSTRACTThe influence of pulp carryover on the efficiency of the xylanase (X) treatment of industrial unbleached and oxygen-delignified eucalypt kraft pulps (A1 and A2 pulps, with kappa number (KN) values of 16 and 10, respectively), collected at the same pulp mill, was studied regarding the consumption of bleaching chemicals and pulp bleachability. Another non-oxygen-delignified eucalyptus kraft pulp of KN 13 was received after the extended cooking from a different pulp mill (pulp B). The assays were performed with both lab-washed (carryove...
Source: Applied Microbiology and Biotechnology - February 15, 2024 Category: Microbiology Authors: Jos é M S Matos Dmitry V Evtuguin Ant ónio P Mendes de Sousa M Gra ça V S Carvalho Source Type: research

Nitric oxide synthase expression in Pseudomonas koreensis MME3 improves plant growth promotion traits
In this study, the plant growth-promoting rhizobacteria Pseudomonas koreensis MME3 was genetically modified to express a nitric oxide synthase of Synechococcus SyNOS, an atypical enzyme with a globin domain that converts nitric oxide to nitrate. A cassette for constitutive expression of synos was introduced as a single insertion into the genome of P. koreensis MME3 using a miniTn7 system. The resulting recombinant strain MME3:SyNOS showed improved growth, motility, and biofilm formation. The impact of MME3:SyNOS inoculation on Brachypodium distachyon growth and N uptake and use efficiencies under different N availability s...
Source: Applied Microbiology and Biotechnology - February 15, 2024 Category: Microbiology Authors: Mar ía M Labarthe Guillermo A Maroniche Lorenzo Lamattina Cecilia M Creus Source Type: research

Applied microbiology of the phyllosphere
Appl Microbiol Biotechnol. 2024 Feb 15;108(1):211. doi: 10.1007/s00253-024-13042-4.ABSTRACTThe phyllosphere, or plant leaf surface, represents a microbial ecosystem of considerable size, holding extraordinary biodiversity and enormous potential for the discovery of new products, tools, and applications in biotechnology, agriculture, medicine, and elsewhere. This mini-review highlights the applied microbiology of the phyllosphere as an original field of study concerning itself with the genes, gene products, natural compounds, and traits that underlie phyllosphere-specific adaptations and services that have commercial and ec...
Source: Applied Microbiology and Biotechnology - February 15, 2024 Category: Microbiology Authors: Lorena I Rangel Johan H J Leveau Source Type: research

A study on the association between gut microbiota, inflammation, and type 2 diabetes
Appl Microbiol Biotechnol. 2024 Feb 15;108(1):213. doi: 10.1007/s00253-024-13041-5.ABSTRACTType 2 diabetes mellitus (T2DM) was reported to be associated with impaired immune response and alterations in microbial composition and function. However, the underlying mechanism remains elusive. To investigate the association among retinoic acid-inducible gene-I-like receptors (RLRs) signaling pathway, intestinal bacterial microbiome, microbial tryptophan metabolites, inflammation, and a longer course of T2DM, 14 patients with T2DM and 7 healthy controls were enrolled. 16S rRNA amplicon sequencing and untargeted metabolomics were ...
Source: Applied Microbiology and Biotechnology - February 15, 2024 Category: Microbiology Authors: Nannan Liu Xuehua Yan Bohan Lv Yanxiang Wu Xuehong Hu Chunyan Zheng Siyu Tao Ruxue Deng Jinfang Dou Binfang Zeng Guangjian Jiang Source Type: research

Engineering Cas9: next generation of genomic editors
Appl Microbiol Biotechnol. 2024 Feb 14;108(1):209. doi: 10.1007/s00253-024-13056-y.ABSTRACTThe Cas9 endonuclease of the CRISPR/Cas type IIA system from Streptococcus pyogenes is the heart of genome editing technology that can be used to treat human genetic and viral diseases. Despite its large size and other drawbacks, S. pyogenes Cas9 remains the most widely used genome editor. A vast amount of research is aimed at improving Cas9 as a promising genetic therapy. Strategies include directed evolution of the Cas9 protein, rational design, and domain swapping. The first generation of Cas9 editors comes directly from the wild-...
Source: Applied Microbiology and Biotechnology - February 14, 2024 Category: Microbiology Authors: Maxim A Kovalev Artem I Davletshin Dmitry S Karpov Source Type: research

High-yield BMP2 expression in rice cells via CRISPR and endogenous αAmy3 promoter
This study explored the efficacy of utilizing an endogenous αAmy3 promoter for the expression of a bioactive pharmaceutical protein, specifically the mature region of human bone morphogenetic protein 2 (hBMP2m). Utilizing a refined CRISPR/Cas9-mediated intron-targeting insertion technique, which incorporates an artificial 3' splicing site upstream of the target gene, we achieved a transformation efficiency of 13.5% in rice calli that carried the rice-codon optimized mature region of hBMP2 cDNA (rhBMP2m) in the αAmy3 intron 1. Both homozygous and heterozygous rhBMP2m knock-in rice suspension cell lines were generated. The...
Source: Applied Microbiology and Biotechnology - February 14, 2024 Category: Microbiology Authors: Thi Mai Nguyen Pei-Yi Wu Chih-Hung Chang Li-Fen Huang Source Type: research

Enzymatic upcycling of wild-simulated ginseng leaves for enhancing biological activities and compound K
In this study, CK production in WGSL reached 25.9 ± 1.0 mg/g extract following treatment with Viscozyme, Celluclast 1.5 L, Pectinex Ultra SP-L, and their combination. The antioxidant activities indicated by oxygen radical absorbance capacity, ferric reducing antioxidant power, and ABTS- and DPPH radical scavenging activity of enzyme-treated WSGL were enhanced 1.69-, 2.51-, 2.88-, and 1.80-fold, respectively, compared to non-treated WSGL. Furthermore, the CK-enriched WSGL demonstrated a 1.94-fold decrease in SA-β-galactosidase expression in human dermal fibroblasts and a 3.8-fold enhancement of inhibition of nitric oxide ...
Source: Applied Microbiology and Biotechnology - February 14, 2024 Category: Microbiology Authors: Juho Lim Hayeong Kim Gha-Hyun J Kim Taeyoon Kim Choon Gil Kang Seung Wook Kim Doman Kim Source Type: research

A short guide on blue fluorescent proteins: limits and perspectives
Appl Microbiol Biotechnol. 2024 Feb 14;108(1):208. doi: 10.1007/s00253-024-13012-w.ABSTRACTThe advent of the so-called colorful biology era is in line with the discovery of fluorescent proteins (FPs), which can be widely used to detect the intracellular locations of macromolecules or to determine the abundance of metabolites in organelles. The application of multiple FPs that emit different spectra and colors could be implemented to precisely evaluate cellular events. FPs were initially established with the emergence of the green fluorescent protein (GFP) from jellyfish. Red fluorescent proteins (RFPs) from marine anemones...
Source: Applied Microbiology and Biotechnology - February 14, 2024 Category: Microbiology Authors: Pil-Won Seo Geun-Joong Kim Jeong-Sun Kim Source Type: research

Squalene production under oxygen limitation by Schizochytrium sp. S31 in different cultivation systems
Appl Microbiol Biotechnol. 2024 Feb 13;108(1):201. doi: 10.1007/s00253-024-13051-3.ABSTRACTThe triterpene squalene is widely used in the food, cosmetics and pharmaceutical industries due to its antioxidant, antistatic and anti-carcinogenic properties. It is usually obtained from the liver of deep sea sharks, which are facing extinction. Alternative production organisms are marine protists from the family Thraustochytriaceae, which produce and store large quantities of various lipids. Squalene accumulation in thraustochytrids is complex, as it is an intermediate in sterol biosynthesis. Its conversion to squalene 2,3-epoxide...
Source: Applied Microbiology and Biotechnology - February 13, 2024 Category: Microbiology Authors: Lina Sch ütte Patrick G Hanisch Nina Scheler Katharina C Hab öck Robert Huber Franziska Ersoy Ralf G Berger Source Type: research

Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing
Appl Microbiol Biotechnol. 2024 Feb 13;108(1):205. doi: 10.1007/s00253-024-13055-z.ABSTRACTAeromonas species (spp.) are well-known fish pathogens, several of which have been recognized as emerging human pathogens. The organism is capable of causing a wide spectrum of diseases in humans, ranging from gastroenteritis, wound infections, and septicemia to devastating necrotizing fasciitis. The systemic form of infection is often fatal, particularly in patients with underlying chronic diseases. Indeed, recent trends demonstrate rising numbers of hospital-acquired Aeromonas infections, especially in immuno-compromised individual...
Source: Applied Microbiology and Biotechnology - February 13, 2024 Category: Microbiology Authors: Blake Neil Gabrielle L Cheney Jason A Rosenzweig Jian Sha Ashok K Chopra Source Type: research