Applied Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Novel anti-inflammatory diketopiperazine alkaloids from the marine-derived fungus Penicillium brasilianum
Appl Microbiol Biotechnol. 2024 Feb 5;108(1):194. doi: 10.1007/s00253-024-13026-4.ABSTRACTDiketopiperazine alkaloids have proven the most abundant heterocyclic alkaloids up to now, which usually process diverse scaffolds and rich biological activities. In our search for bioactive diketopiperazine alkaloids from marine-derived fungi, two novel diketopiperazine alkaloids, penipiperazine A (1) and its biogenetically related new metabolite (2), together with a known analogue neofipiperzine C (3), were obtained from the strain Penicillium brasilianum. Their planar structures and absolute configurations were elucidated by extens...
Source: Applied Microbiology and Biotechnology - February 5, 2024 Category: Microbiology Authors: Ya-Hui Zhang Hui-Fang Du Yun-Feng Liu Fei Cao Du-Qiang Luo Chang-Yun Wang Source Type: research

Recombinant mannan-binding lectin magnetic beads increase pathogen detection in immunocompromised patients
Appl Microbiol Biotechnol. 2024 Feb 3;108(1):193. doi: 10.1007/s00253-024-13019-3.ABSTRACTThe microbiological diagnosis of infection for hematological malignancy patients receiving chemotherapy or allogeneic hematopoietic stem cell transplantation (allo-HSCT) patients relies primarily on standard microbial culture, especially blood culture, which has many shortcomings, such as having low positive rates, being time-consuming and having a limited pathogenic spectrum. In this prospective observational self-controlled test accuracy study, blood, cerebrospinal fluid (CSF), and bronchoalveolar lavage fluid (BALF) samples were co...
Source: Applied Microbiology and Biotechnology - February 3, 2024 Category: Microbiology Authors: Chen Xiao-Ping Zheng Hao Feng Ru-Li Lu Jin-Xing Dong Yu-Jun Liang Ze-Yin Source Type: research

Bacterial crude oil and polyaromatic hydrocarbon degraders from Kazakh oil fields as barley growth support
Appl Microbiol Biotechnol. 2024 Feb 2;108(1):189. doi: 10.1007/s00253-024-13010-y.ABSTRACTBacterial strains of the genera Arthrobacter, Bacillus, Dietzia, Kocuria, and Micrococcus were isolated from oil-contaminated soils of the Balgimbaev, Dossor, and Zaburunye oil fields in Kazakhstan. They were selected from 1376 isolated strains based on their unique ability to use crude oil and polyaromatic hydrocarbons (PAHs) as sole source of carbon and energy in growth experiments. The isolated strains degraded a wide range of aliphatic and aromatic components from crude oil to generate a total of 170 acid metabolites. Eight metabo...
Source: Applied Microbiology and Biotechnology - February 2, 2024 Category: Microbiology Authors: Kuralay Yessentayeva Anne Reinhard Ramza Berzhanova Togzhan Mukasheva Tim Urich Annett Mikolasch Source Type: research

Effect of micro-aeration on syntrophic and methanogenic activity in anaerobic sludge
Appl Microbiol Biotechnol. 2024 Feb 2;108(1):192. doi: 10.1007/s00253-023-12969-4.ABSTRACTMicro-aeration was shown to improve anaerobic digestion (AD) processes, although oxygen is known to inhibit obligate anaerobes, such as syntrophic communities of bacteria and methanogens. The effect of micro-aeration on the activity and microbial interaction in syntrophic communities, as well as on the potential establishment of synergetic relationships with facultative anaerobic bacteria (FAB) or aerobic bacteria (AB), was investigated. Anaerobic sludge was incubated with ethanol and increasing oxygen concentrations (0-5% in the head...
Source: Applied Microbiology and Biotechnology - February 2, 2024 Category: Microbiology Authors: Bruno P Morais Carla P Magalh ães Gilberto Martins Maria Alcina Pereira Ana J Cavaleiro Source Type: research

Cleavage of natural rubber by rubber oxygenases in Gram-negative bacteria
We describe the putative evolution of rubber oxygenases and confirm the presence of a third subgroup of Rox-related proteins (RoxCs), the biological function of which remains, however, unclear. We summarize the knowledge on the taxonomic position of Steroidobacter cummioxidans 35Y and related species. Comparison of genomic and biochemical features of strain 35Y with other species of the genus Steroidobacter suggests that strain 35Y represents a species of a novel genus for which the designation Aurantibaculum gen. nov. is proposed. A short summary on the capabilities of NR-degrading consortia, that could be superior in bio...
Source: Applied Microbiology and Biotechnology - February 2, 2024 Category: Microbiology Authors: Tulika Prakash Sandhya R Yadav Marius B ürger Dieter Jendrossek Source Type: research

Rapid screening of point mutations by mismatch amplification mutation assay PCR
In this study, mismatch amplification mutation assay (MAMA) PCR was used to detect point mutation and screen saturation mutation library. In order to fine-tune the expression of odhA encoding 2-oxoglutarate dehydrogenase E1 component, a saturating mutant library of the RBS of odhA was created in Corynebacterium glutamicum P12 based on the CRISPR-Cas2a genome editing system, which increased the L-proline production by 81.3%. MAMA PCR was used to filter out 42% of the non-mutant transformants in the mutant library, which effectively reduced the workload of the subsequent fermentation test and the number of sequenced samples....
Source: Applied Microbiology and Biotechnology - February 2, 2024 Category: Microbiology Authors: Feng Zhang Zhen Yang Liu Shuai Liu Wei Guo Zhang Bing Bing Wang Chang Lon Li Jian Zhong Xu Source Type: research

Rerouting phytosterol degradation pathway for directed androst-1,4-diene-3,17-dione microbial bioconversion
In this study, the catabolic switch between the C19- and C22-steroidal subpathways was investigated in resting cells of Mycobacterium neoaurum NRRL B-3805, and a dose-dependent transcriptional response toward the induction of phytosterol with increased concentrations was found in the putative node enzymes including ChoM2, KstD1, OpccR, Sal, and Hsd4A. Aldolase Sal presented a dominant role in the C22 steroidal side-chain cleavage, and the byproduct was eliminated after sequential deletion of opccR and sal. Meanwhile, the molar yield of androst-1,4-diene-3,17-dione (ADD) was increased from 59.4 to 71.3%. With the regard of ...
Source: Applied Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Authors: Xia Ke Jia-Hao Cui Qi-Jie Ren Tong Zheng Xin-Xin Wang Zhi-Qiang Liu Yu-Guo Zheng Source Type: research

Functional and structural analysis of a cyclization domain in a cyclic β-1,2-glucan synthase
Appl Microbiol Biotechnol. 2024 Feb 1;108(1):187. doi: 10.1007/s00253-024-13013-9.ABSTRACTCyclic β-1,2-glucan synthase (CGS) is a key enzyme in production of cyclic β-1,2-glucans (CβGs) which are involved in bacterial infection or symbiosis to host organisms. Nevertheless, a mechanism of cyclization, the final step in the CGS reaction, has not been fully understood. Here we performed functional and structural analyses of the cyclization domain of CGS alone from Thermoanaerobacter italicus (TiCGSCy). We first found that β-glucosidase-resistant compounds are produced by TiCGSCy with linear β-1,2-glucans as substrates. T...
Source: Applied Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Authors: Nobukiyo Tanaka Ryotaro Saito Kaito Kobayashi Hiroyuki Nakai Shogo Kamo Kouji Kuramochi Hayao Taguchi Masahiro Nakajima Tomoko Masaike Source Type: research

Sensing chemical-induced DNA damage using CRISPR/Cas9-mediated gene-deletion yeast-reporter strains
In this study, we developed both chromosomally integrated and single-copy plasmid-based reporter assays in budding yeast using a RNR3 promoter-driven luciferase gene. These assays were designed to compare the response to genotoxic chemicals with a pre-established multicopy plasmid-based assay. Despite exhibiting the lowest luciferase activity, the chromosomally integrated reporter assay showed the highest fold induction (i.e., the ratio of luciferase activity in the presence and absence of the chemical) compared with the established plasmid-based assay. Using CRISPR/Cas9 technology, we generated mutants with single- or dou...
Source: Applied Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Authors: Kosuke Yamamoto Shintaro Tochikawa Yuuki Miura Shogo Matsunobu Yuu Hirose Toshihiko Eki Source Type: research

Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes
Appl Microbiol Biotechnol. 2024 Jan 30;108(1):185. doi: 10.1007/s00253-023-12900-x.ABSTRACTThe application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released...
Source: Applied Microbiology and Biotechnology - January 30, 2024 Category: Microbiology Authors: Sandra Vojnovic Ivana Aleksic Tatjana Ilic-Tomic Milena Stevanovic Jasmina Nikodinovic-Runic Source Type: research

Identification of a novel thermostable transaminase and its application in L-phosphinothricin biosynthesis
Appl Microbiol Biotechnol. 2024 Jan 30;108(1):184. doi: 10.1007/s00253-024-13023-7.ABSTRACTTransaminase (TA) is a crucial biocatalyst for enantioselective production of the herbicide L-phosphinothricin (L-PPT). The use of enzymatic cascades has been shown to effectively overcome the unfavorable thermodynamic equilibrium of TA-catalyzed transamination reaction, also increasing demand for TA stability. In this work, a novel thermostable transaminase (PtTA) from Pseudomonas thermotolerans was mined and characterized. The PtTA showed a high specific activity (28.63 U/mg) towards 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric ac...
Source: Applied Microbiology and Biotechnology - January 30, 2024 Category: Microbiology Authors: Han-Lin Liu Pu-Hong Yi Jia-Min Wu Feng Cheng Zhi-Qiang Liu Li-Qun Jin Ya-Ping Xue Yu-Guo Zheng Source Type: research

Recombinant therapeutic proteins degradation and overcoming strategies in CHO cells
Appl Microbiol Biotechnol. 2024 Jan 29;108(1):182. doi: 10.1007/s00253-024-13008-6.ABSTRACTMammalian cell lines are frequently used as the preferred host cells for producing recombinant therapeutic proteins (RTPs) having post-translational modified modification similar to those observed in proteins produced by human cells. Nowadays, most RTPs approved for marketing are produced in Chinese hamster ovary (CHO) cells. Recombinant therapeutic antibodies are among the most important and promising RTPs for biomedical applications. One of the issues that occurs during development of RTPs is their degradation, which caused by a va...
Source: Applied Microbiology and Biotechnology - January 29, 2024 Category: Microbiology Authors: Shao-Lei Geng Xiao-Jie Zhao Xi Zhang Ji-Hong Zhang Chun-Liu Mi Tian-Yun Wang Source Type: research

Transcriptomic analyses of Vibrio parahaemolyticus under the phenyllactic acid stress
In this study, V. parahaemolyticus ATCC17802 (Vp17802) was cultured under the stress of 1/2MIC PLA, and then the antibacterial mechanisms were explored via transcriptomics. The minimum inhibitory concentration (MIC) of PLA against Vp17802 was 3.2 mg/mL, and the time-kill analysis resulted that Vp17802 was inhibited. PLA was able to destroy the bacterial membrane, leading to the leakage of intracellular substances and decline of ATP levels. The RNA-sequencing analysis results indicated that 1616 significantly differentially expressed genes were identified, among which 190 were up-regulated and 1426 were down-regulated. Down...
Source: Applied Microbiology and Biotechnology - January 29, 2024 Category: Microbiology Authors: Yilin Lin Meimei Fang Jun Liu Yehui Zhang Yigang Yu Source Type: research

Performance insights into spray-dryer microencapsulated Bacillus thuringiensis cry pesticidal proteins with gum arabic and maltodextrin for effective pest control
Appl Microbiol Biotechnol. 2024 Jan 29;108(1):181. doi: 10.1007/s00253-023-12990-7.ABSTRACTBacillus thuringiensis (Bt) produces crystals composed mainly of Cry pesticidal proteins with insecticidal activity against pests but are highly susceptible to degradation by abiotic factors. In this sense, encapsulation techniques are designed to improve their performance and lifetime. However, the effects of polymeric matrix encapsulation such as gum arabic and maltodextrin by spray-dryer in the mechanisms of action of Bt kurstaki and Bt aizawai are unknown. We analyzed crystal solubilization, protoxin activation, and receptor bind...
Source: Applied Microbiology and Biotechnology - January 29, 2024 Category: Microbiology Authors: Jhones Luiz de Oliveira Isabel G ómez Jorge S ánchez Mario Sober ón Ricardo Antonio Polanczyk Alejandra Bravo Source Type: research

Recombinantly expressed rhFEB remodeled the skin defect of db/db mice
Appl Microbiol Biotechnol. 2024 Jan 29;108(1):183. doi: 10.1007/s00253-024-13021-9.ABSTRACTFibronectin (FN) and collagen are vital components of the extracellular matrix (ECM). These proteins are essential for tissue formation and cell alignment during the wound healing stage. In particular, FN interacts with collagens to activate various intracellular signaling pathways to maintain ECM stability. A novel recombinant extra domain-B fibronectin (EDB-FN)-COL3A1 fusion protein (rhFEB) was designed to mimic the ECM to promote chronic and refractory skin ulcer wound healing. rhFEB significantly enhanced cell adhesion and migrat...
Source: Applied Microbiology and Biotechnology - January 29, 2024 Category: Microbiology Authors: Xiaomin Li Xinliang Mao Jianhang Cong Qirong Zhang Wenjie Chen Kunjun Yan Yadong Huang Dun Su Qi Xiang Source Type: research