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A type III polyketide synthase cluster in the phylum Planctomycetota is involved in alkylresorcinol biosynthesis
In this study, we performed systematic bioinformatic analyses based on the genomes of all 131 described members of the current phylum focusing on the identification of type III polyketide synthase (PKS) genes. Type III PKSs are versatile enzymes involved in the biosynthesis of a wide array of structurally diverse natural products with potent biological activities. We identified 96 putative type III PKS genes of which 58 are encoded in an operon with genes encoding a putative oxidoreductase and a methyltransferase. Sequence similarities on protein level and the genetic organization of the operon point towards a functional l...
Source: Applied Microbiology and Biotechnology - February 26, 2024 Category: Microbiology Authors: Lars Milke Moses Kabuu Ren è Zschoche Jochem G ätgens Karin Krumbach Kim-Loreen Carlstedt Carmen E Wurzbacher Sven Balluff Christine Beemelmanns Christian Jogler Jan Marienhagen Nicolai Kallscheuer Source Type: research

Genetic structure and population diversity of Phytophthora infestans strains in Pacific western Canada
Appl Microbiol Biotechnol. 2024 Feb 26;108(1):237. doi: 10.1007/s00253-024-13040-6.ABSTRACTLate blight caused by Phytophthora infestans is an economically important disease of potato and tomato worldwide. In Canada, an increase in late blight incidence and severity coincided with changes in genetic composition of P. infestans. We monitored late blight incidence on tomato and potato in Pacific western and eastern Canada between 2019 and 2022, identified genotypes of P. infestans, and examined their population genetic diversity. We identified four major existing genotypes US11, US17, US8, and US23 as well as 25 new genotypes...
Source: Applied Microbiology and Biotechnology - February 26, 2024 Category: Microbiology Authors: Segun Babarinde Rishi R Burlakoti Rick D Peters Khalil Al-Mughrabi Amy Novinscak Sanjib Sapkota Balakrishnan Prithiviraj Source Type: research

Correlation study on microbial communities and volatile flavor compounds in cigar tobacco leaves of diverse origins
Appl Microbiol Biotechnol. 2024 Feb 26;108(1):236. doi: 10.1007/s00253-024-13032-6.ABSTRACTTo elucidate the significant influence of microorganisms on geographically dependent flavor formation by analyzing microbial communities and volatile flavor compounds (VFCs) in cigar tobacco leaves (CTLs) obtained from China, Dominica, and Indonesia. Microbiome analysis revealed that the predominant bacteria in CTLs were Staphylococcus, Aerococcus, Pseudomonas, and Lactobacillus, while the predominant fungi were Aspergillus, Wallemia, and Sampaiozyma. The microbial communities of CTLs from different origins differed to some extent, a...
Source: Applied Microbiology and Biotechnology - February 26, 2024 Category: Microbiology Authors: Haiqing Wang Dongfeng Guo Mingzhu Zhang Guanglong Wu Yaqi Shi Jinglong Zhou Naihong Ding Xiangsong Chen Xingjiang Li Source Type: research

Recent advance of microbial mercury methylation in the environment
Appl Microbiol Biotechnol. 2024 Feb 26;108(1):235. doi: 10.1007/s00253-023-12967-6.ABSTRACTMethylmercury formation is mainly driven by microbial-mediated process. The mechanism of microbial mercury methylation has become a crucial research topic for understanding methylation in the environment. Pioneering studies of microbial mercury methylation are focusing on functional strain isolation, microbial community composition characterization, and mechanism elucidation in various environments. Therefore, the functional genes of microbial mercury methylation, global isolations of Hg methylation strains, and their methylation pot...
Source: Applied Microbiology and Biotechnology - February 26, 2024 Category: Microbiology Authors: Xuya Peng Yan Yang Shu Yang Lei Li Liyan Song Source Type: research

Characterization and immunological effect of outer membrane vesicles from Pasteurella multocida on macrophages
In this study, OMVs were isolated from P. multocida using an ultrafiltration concentration technique, and their morphology, protein constitution, and immunomodulatory properties in RAW264.7 cells were studied. Transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) revealed that the OMVs exhibited typical spherical and bilayered lipid vesicular architecture, exhibiting an average diameter of approximately 147.5 nm. The yield of OMVs was 2.6 × 1011 particles/mL. Proteomic analysis revealed a high abundance of membrane-associated proteins within P. multocida OMVs, with the capability to instigate the...
Source: Applied Microbiology and Biotechnology - February 26, 2024 Category: Microbiology Authors: Jiaying Sun Yee Huang Xuefeng Li Xiangfei Xu Xuemei Cui Fangjiao Hao Quanan Ji Chun Chen Guolian Bao Yan Liu Source Type: research

An M cell-targeting recombinant L. lactis vaccine against four H. pylori adhesins
Appl Microbiol Biotechnol. 2024 Feb 23;108(1):231. doi: 10.1007/s00253-024-13070-0.ABSTRACTThe acidic environment and enzyme degradation lead to oral vaccines often having little immune effect. Therefore, it is an attractive strategy to study an effective and safe oral vaccine delivery system that can promote gastrointestinal mucosal immune responses and inhibit antigen degradation. Moreover, the antigens uptake by microfold cells (M cells) is the determining step in initiating efficient immune responses. Therefore, M cell-targeting is one promising approach for enhancing oral vaccine potency. In the present study, an M ce...
Source: Applied Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Authors: Furui Zhang Tianyi Shi Zhen Zhang Shue Wang Jing Liu Yonghong Li Xuequan Wang Kunmei Liu Le Guo Source Type: research

Tailor-made solvents for microbial carotenoids recovery
Appl Microbiol Biotechnol. 2024 Feb 24;108(1):234. doi: 10.1007/s00253-024-13049-x.ABSTRACTIn recent years, microbial carotenoids have emerged as a promising alternative for the pharmaceutical and food industries, particularly in promoting human health due to their potent antioxidant and antimicrobial properties. Microbial carotenoids, particularly those produced by yeast, bacteria, and microalgae, are synthesized intracellularly, requiring the use of solvents for their effective extraction and recovery. The conventional use of toxic volatile organic solvents (VOCs) like hexane, petroleum ether, and dimethyl sulfoxide in t...
Source: Applied Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Authors: Cassamo U Mussagy Henua U Hucke Nataly F Ramos Helena F Ribeiro Mariana B Alves Ahmad Mustafa Jorge F B Pereira Fabiane O Farias Source Type: research

Characterization of a recombinant Aspergillus niger GZUF36 lipase immobilized by ionic liquid modification strategy
In this study, a recombinant extracellular lipase from Aspergillus niger GZUF36 (PEXANL1) expressed in Pichia pastoris GS115 was immobilized on ionic liquid-modified magnetic nano ferric oxide (Fe3O4@SiO2@ILs) via electrostatic and hydrophobic interaction. The morphology, structure, and properties of Fe3O4@SiO2@ILs and immobilized PEXANL1 were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, x-ray diffraction, vibration sample magnetometer, and zeta potential analysis. Under optimized conditions, the immobilization efficiency and activity recovery of immobilized PEXANL1 were 52 ± 2% ...
Source: Applied Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Authors: Shuqi Xing Jia Long Wei Xie Chaocheng Luo Laping He Cuiqin Li Xuefeng Zeng Source Type: research