Applied Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

High-level biosynthesis of enantiopure germacrene D in yeast
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-18. doi: 10.1007/s00253-023-12885-7. Epub 2024 Jan 6.ABSTRACTGermacrene D, a sesquiterpenoid compound found mainly in plant essential oils at a low level as (+) and/or (-) enantiomeric forms, is an ingredient for the fragrance industry, but a process for the sustainable supply of enantiopure germacrene D is not yet established. Here, we demonstrate metabolic engineering in yeast (Saccharomyces cerevisiae) achieving biosynthesis of enantiopure germacrene D at a high titer. To boost farnesyl pyrophosphate (FPP) flux for high-level germacrene D biosynthesis, a background yeast chas...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Shubha Sharma Seema Chaurasia Sandeep Dinday Gaurav Srivastava Anamika Singh Chandan Singh Chanotiya Sumit Ghosh Source Type: research

Recent findings in methanotrophs: genetics, molecular ecology, and biopotential
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-21. doi: 10.1007/s00253-023-12978-3. Epub 2024 Jan 6.ABSTRACTThe potential consequences for mankind could be disastrous due to global warming, which arises from an increase in the average temperature on Earth. The elevation in temperature primarily stems from the escalation in the concentration of greenhouse gases (GHG) such as CO2, CH4, and N2O within the atmosphere. Among these gases, methane (CH4) is particularly significant in driving alterations to the worldwide climate. Methanotrophic bacteria possess the distinctive ability to employ methane as both as source of carbon an...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Fatemeh Ahmadi Maximilian Lackner Source Type: research

Discovery and biochemical characterization of thermostable glycerol oxidases
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-14. doi: 10.1007/s00253-023-12883-9. Epub 2024 Jan 6.ABSTRACTAlditol oxidases are promising tools for the biocatalytic oxidation of glycerol to more valuable chemicals. By integrating in silico bioprospecting with cell-free protein synthesis and activity screening, an effective pipeline was developed to rapidly identify enzymes that are active on glycerol. Three thermostable alditol oxidases from Actinobacteria Bacterium, Streptomyces thermoviolaceus, and Thermostaphylospora chromogena active on glycerol were discovered. The characterization of these three flavoenzymes demonstra...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Lars L Santema Laura Rotilio Ruite Xiang Gwen Tjallinks Victor Guallar Andrea Mattevi Marco W Fraaije Source Type: research

Exploration of a multifunctional biocontrol agent Streptomyces sp. JCK-8055 for the management of apple fire blight
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-19. doi: 10.1007/s00253-023-12874-w. Epub 2024 Jan 6.ABSTRACTApple fire blight, caused by the bacterium Erwinia amylovora, is a devastating disease of apple and pear trees. Biological control methods have attracted much attention from researchers to manage plant diseases as they are eco-friendly and viable alternatives to synthetic pesticides. Herein, we isolated Streptomyces sp. JCK-8055 from the root of pepper and investigated its mechanisms of action against E. amylovora. Streptomyces sp. JCK-8055 produced aureothricin and thiolutin, which antagonistically affect E. amylovora...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Loan Thi Thanh Nguyen Ae Ran Park Ve Van Le Inmin Hwang Jin-Cheol Kim Source Type: research

The application of bacteria-derived dehydrogenases and oxidases in the synthesis of gold nanoparticles
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-13. doi: 10.1007/s00253-023-12853-1. Epub 2024 Jan 6.ABSTRACTIn this work the green synthesis of gold nanoparticles (Au-NPs) using the oxidoreductive enzymes Myriococcum thermophilum cellobiose dehydrogenase (Mt CDH), Glomerella cingulata glucose dehydrogenase (Gc GDH), and Aspergillus niger glucose oxidase (An GOX)) as bioreductants was investigated. The influence of reaction conditions on the synthesis of Au-NPs was examined and optimised. The reaction kinetics and the influence of Au ions on the reaction rate were determined. Based on the kinetic study, the mechanism of Au-NP...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Lela Martinaga Roland Ludwig Iva Rezi ć Martina Andlar Dietmar Pum Ana Vrsalovi ć Presečki Source Type: research

Lonicera japonica protects Pelodiscus sinensis by inhibiting the biofilm formation of Aeromonas hydrophila
In this study, we isolated an A. hydrophila strain B3 from diseased soft-shelled turtle (Pelodiscus sinensis), which is one of the most commercially significant freshwater farmed reptiles in East Asia, and found that A. hydrophila was its dominant pathogen. To better understand the inhibition effect and action mechanism of Chinese herbs on A. hydrophila, we conducted Chinese herbs screening and found that Lonicera japonica had a significant antibacterial effect on A. hydrophila B3. Experimental therapeutics of L. japonica on soft-shelled turtle showed that the supplement of 1% L. japonica to diet could significantly upregu...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Li-Chao Huo Nai-Yu Liu Chao-Jie Wang Yi Luo Jing-Xia Liu Source Type: research

Enzymatic synthesis of phenolic acid glucosyl esters to test activities on cholangiocarcinoma cells
In this study, Os9BGlu31 was used to synthesize eight phenolic acid glucosyl esters, which were evaluated for activities in cholangiocarcinoma cells. The transglycosylation products of Os9BGlu31 wild type and its mutant variants were detected, produced on a milligram scale, and purified, and their structures were characterized by NMR spectroscopy. The transglycosylation products were evaluated by antioxidant and anti-proliferative assays, followed by an anti-migration assay for the selected phenolic acid glucosyl ester. Os9BGlu31 mutants produced higher yield and activity than wild-type enzymes on phenolic acids to produce...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Eko Suyanto Jaggaiah N Gorantla Maniganda Santi Fatchiyah Fatchiyah Mariena Ketudat-Cairns Chutima Talabnin James R Ketudat Cairns Source Type: research

Metabolic engineering of Shewanella oneidensis to produce glutamate and itaconic acid
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-10. doi: 10.1007/s00253-023-12879-5. Epub 2024 Jan 6.ABSTRACTShewanella oneidensis is a gram-negative bacterium known for its unique respiratory capabilities, which allow it to utilize a wide range of electron acceptors, including solid substrates such as electrodes. For a future combination of chemical production and electro-fermentation, the goal of this study was to expand its product spectrum. S. oneidensis was metabolically engineered to optimize its glutamate production and to enable production of itaconic acid. By deleting the glutamate importer gltS for a reduced glutama...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Hannah Wohlers Laura Zentgraf Lisa van der Sande Dirk Holtmann Source Type: research

Distinct alterations of gut microbiota between viral- and non-viral-related hepatocellular carcinoma
This study was conducted to identify a gut microbiota signature in differentiating between viral-related HCC (Viral-HCC) and non-hepatitis B-, non-hepatitis C-related HCC (NBNC-HCC). Fecal specimens were obtained from 16 healthy controls, 33 patients with viral-HCC (17 and 16 cases with hepatitis B virus (HBV) and hepatitis C virus (HCV) infection, respectively), and 18 patients with NBNC-HCC. Compositions of fecal microbiota were assessed by 16S rRNA sequencing. Bioinformatic analysis was performed by the DADA2 pipeline in the R program. Significantly different genera from the top 50 relative abundance were used to classi...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Thananya Jinato Songtham Anuntakarun Nantawat Satthawiwat Natthaya Chuaypen Pisit Tangkijvanich Source Type: research

Effects of fecal microbial transplantation on police performance and transportation stress in Kunming police dogs
This study aimed to investigate the effects of FMT on performance and gut microflora in Kunming police dogs. Twenty Wolf Cyan dogs were randomly assigned to receive physiological saline or fecal suspension at low, medium, or high doses through oral gavage for 14 days. Growth performance, police performance, serum biochemical profiling, and gut microflora were determined 2-week post-FMT. Dogs after FMT treatment were also subjected to an hour road transportation and then were evaluated for serum stress indicators. Overall, FMT enhanced the growth performance and alleviated diarrhea rate in Kunming dogs with the greatest eff...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Qiu-Ye Lin Jin-Jing Du Hu Xu Ming-Kui Lv Le Xu Jie Li Zhen-Hui Cao Source Type: research

Development of multiplex allele-specific RT-qPCR assays for differentiation of SARS-CoV-2 Omicron subvariants
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-17. doi: 10.1007/s00253-023-12941-2. Epub 2024 Jan 6.ABSTRACTQuick differentiation of current circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmissions. However, the widely applied gene sequencing method is time-consuming and costly especially when facing recombinant variants, because a large part or whole genome sequencing is required. Allele-specific reverse transcriptase real time RT-PCR (RT-qPCR) represents a quick and cost-effective method for SNP (single nucleotide polymorphism) genotyping and has been successfully...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Jianguo Li Ruiling Cheng Zixin Bian Jiahui Niu Juan Xia Guoli Mao Hulong Liu Changxin Wu Chunyan Hao Source Type: research

Pathway crosstalk between the central metabolic and heme biosynthetic pathways in Phanerochaete chrysosporium
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-14. doi: 10.1007/s00253-023-12846-0. Epub 2024 Jan 6.ABSTRACTA comprehensive analysis to survey heme-binding proteins produced by the white-rot fungus Phanerochaete chrysosporium was achieved using a biotinylated heme-streptavidin beads system. Mitochondrial citrate synthase (PcCS), glyceraldehyde 3-phosphate dehydrogenase (PcGAPDH), and 2-Cys thioredoxin peroxidase (mammalian HBP23 homolog) were identified as putative heme-binding proteins. Among these, PcCS and PcGAPDH were further characterized using heterologously expressed recombinant proteins. Difference spectra of PcCS ti...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Daisuke Miura Ryoga Tsurigami Hiroyuki Kato Hiroyuki Wariishi Motoyuki Shimizu Source Type: research

Analysis of substrate specificity of cytochrome P450 monooxygenases involved in trichothecene toxin biosynthesis
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-21. doi: 10.1007/s00253-023-12950-1. Epub 2024 Jan 6.ABSTRACTTrichothecenes are a structurally diverse family of toxic secondary metabolites produced by certain species of multiple fungal genera. All trichothecene analogs share a core 12,13-epoxytrichothec-9-ene (EPT) structure but differ in presence, absence and types of substituents attached to various positions of EPT. Formation of some of the structural diversity begins early in the biosynthetic pathway such that some producing species have few trichothecene biosynthetic intermediates in common. Cytochrome P450 monooxygenase...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Rosa E Cardoza Susan P McCormick Natalia Mart ínez-Reyes Joaqu ín Rodríguez-Fernández Mark Busman Robert H Proctor Santiago Guti érrez Source Type: research

Prevalence of Escherichia coli in electrogenic biofilm on activated carbon in microbial fuel cell
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-10. doi: 10.1007/s00253-023-12829-1. Epub 2024 Jan 6.ABSTRACTFor a better understanding of the distribution of depth-dependent electrochemically active bacteria at in the anode zone, a customized system in a microbial fuel cell (MFC) packed with granular activated carbon (GAC) was developed and subsequently optimized via electrochemical tests. The constructed MFC system was sequentially operated using two types of matrice solutions: artificially controlled compositions (i.e., artificial wastewater, AW) and solutions obtained directly from actual sewage-treating municipal plants ...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Younggun Yoon Azilah Abd Aziz In Seop Chang Bongkyu Kim Source Type: research

Potassium sodium hydrogen citrate intervention on gut microbiota and clinical features in uric acid stone patients
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-15. doi: 10.1007/s00253-023-12953-y. Epub 2024 Jan 6.ABSTRACTThe high recurrence rate of renal uric acid stone (UAS) poses a significant challenge for urologists, and potassium sodium hydrogen citrate (PSHC) has been proven to be an effective oral dissolution drug. However, no studies have investigated the impact of PSHC on gut microbiota and its metabolites during stone dissolution therapy. We prospectively recruited 37 UAS patients and 40 healthy subjects, of which 12 patients completed a 3-month pharmacological intervention. Fasting vein blood was extracted and mid-stream uri...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Cheng Cao Feng Li Qi Ding Xiaohua Jin Wenjian Tu Hailiang Zhu Mubin Sun Jin Zhu Dongrong Yang Bo Fan Source Type: research