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Glucosylglycerol phosphorylase, a potential novel pathway of microbial glucosylglycerol catabolism
Appl Microbiol Biotechnol. 2024 Feb 16;108(1):214. doi: 10.1007/s00253-024-13035-3.ABSTRACTGlucosylglycerol (GG) is a natural compatible solute that can be synthesized by many cyanobacteria and a few heterotrophic bacteria under high salinity conditions. In cyanobacteria, GG is synthesized by GG-phosphate synthase and GG-phosphate phosphatase, and a hydrolase GGHA catalyzes its degradation. In heterotrophic bacteria (such as some Marinobacter species), a fused form of GG-phosphate phosphatase and GG-phosphate synthase is present, but the cyanobacteria-like degradation pathway is not available. Instead, a phosphorylase GGP,...
Source: Applied Microbiology and Biotechnology - February 16, 2024 Category: Microbiology Authors: Lin Cheng Zhichao Zhang Daling Zhu Quan Luo Xuefeng Lu Source Type: research

In vitro conversion of ellagic acid to urolithin A by different gut microbiota of urolithin metabotype A
In conclusion, the study revealed unique interactions between polyphenols and gut microbiota, deepening our understanding of the relationship between phenolic compounds like EA and the gut microbiota. These findings may contribute to the development of gut bacteria as potential probiotics for further development. KEY POINTS: • Intestinal microbiome involved in ellagic acid metabolism. • Gram-positive bacteria in the intestinal microbiome are crucial for ellagic acid metabolism. • Bifidobacterium longum, Bifidobacterium adolescentis, and Bifidobacterium bifidum participate in ellagic acid metabolism.PMID:38363367 | PM...
Source: Applied Microbiology and Biotechnology - February 16, 2024 Category: Microbiology Authors: Fuxiang He Yingying Bian Yaling Zhao Mengjie Xia Shu Liu Jiajin Gui Xiaoyue Hou Yaowei Fang Source Type: research

Clustered surface amino acid residues modulate the acid stability of GH10 xylanase in fungi
This study not only reported a series of valuable genes but also provided a range of modification targets for enhancing the acid stability of GH10 xylanases. KEY POINTS: • Five acid stable and thermostable GH10 xylanases were reported. • The key amino acid sites, mainly forming two enriched surface regions behind the enzyme active center, were identified responsible for acid stability of GH10 xylanases. • The finding revealed interactive amino acid sites, offering a pathway for synergistic enhancement of both acid stability and thermostability in GH10 xylanase modifications.PMID:38363378 | PMC:PMC10873454 | DOI:10.10...
Source: Applied Microbiology and Biotechnology - February 16, 2024 Category: Microbiology Authors: Yanwei Xia Wei Wang Yaning Wei Chuanxu Guo Sisi Song Siqi Cai Youzhi Miao Source Type: research

In vitro conversion of ellagic acid to urolithin A by different gut microbiota of urolithin metabotype A
In conclusion, the study revealed unique interactions between polyphenols and gut microbiota, deepening our understanding of the relationship between phenolic compounds like EA and the gut microbiota. These findings may contribute to the development of gut bacteria as potential probiotics for further development. KEY POINTS: • Intestinal microbiome involved in ellagic acid metabolism. • Gram-positive bacteria in the intestinal microbiome are crucial for ellagic acid metabolism. • Bifidobacterium longum, Bifidobacterium adolescentis, and Bifidobacterium bifidum participate in ellagic acid metabolism.PMID:38363367 | DO...
Source: Applied Microbiology and Biotechnology - February 16, 2024 Category: Microbiology Authors: Fuxiang He Yingying Bian Yaling Zhao Mengjie Xia Shu Liu Jiajin Gui Xiaoyue Hou Yaowei Fang Source Type: research

Clustered surface amino acid residues modulate the acid stability of GH10 xylanase in fungi
This study not only reported a series of valuable genes but also provided a range of modification targets for enhancing the acid stability of GH10 xylanases. KEY POINTS: • Five acid stable and thermostable GH10 xylanases were reported. • The key amino acid sites, mainly forming two enriched surface regions behind the enzyme active center, were identified responsible for acid stability of GH10 xylanases. • The finding revealed interactive amino acid sites, offering a pathway for synergistic enhancement of both acid stability and thermostability in GH10 xylanase modifications.PMID:38363378 | DOI:10.1007/s00253-024-1304...
Source: Applied Microbiology and Biotechnology - February 16, 2024 Category: Microbiology Authors: Yanwei Xia Wei Wang Yaning Wei Chuanxu Guo Sisi Song Siqi Cai Youzhi Miao Source Type: research

Xylanase treatment of eucalypt kraft pulps: effect of carryover
Appl Microbiol Biotechnol. 2024 Feb 15;108(1):210. doi: 10.1007/s00253-024-13027-3.ABSTRACTThe influence of pulp carryover on the efficiency of the xylanase (X) treatment of industrial unbleached and oxygen-delignified eucalypt kraft pulps (A1 and A2 pulps, with kappa number (KN) values of 16 and 10, respectively), collected at the same pulp mill, was studied regarding the consumption of bleaching chemicals and pulp bleachability. Another non-oxygen-delignified eucalyptus kraft pulp of KN 13 was received after the extended cooking from a different pulp mill (pulp B). The assays were performed with both lab-washed (carryove...
Source: Applied Microbiology and Biotechnology - February 15, 2024 Category: Microbiology Authors: Jos é M S Matos Dmitry V Evtuguin Ant ónio P Mendes de Sousa M Gra ça V S Carvalho Source Type: research

Nitric oxide synthase expression in Pseudomonas koreensis MME3 improves plant growth promotion traits
In this study, the plant growth-promoting rhizobacteria Pseudomonas koreensis MME3 was genetically modified to express a nitric oxide synthase of Synechococcus SyNOS, an atypical enzyme with a globin domain that converts nitric oxide to nitrate. A cassette for constitutive expression of synos was introduced as a single insertion into the genome of P. koreensis MME3 using a miniTn7 system. The resulting recombinant strain MME3:SyNOS showed improved growth, motility, and biofilm formation. The impact of MME3:SyNOS inoculation on Brachypodium distachyon growth and N uptake and use efficiencies under different N availability s...
Source: Applied Microbiology and Biotechnology - February 15, 2024 Category: Microbiology Authors: Mar ía M Labarthe Guillermo A Maroniche Lorenzo Lamattina Cecilia M Creus Source Type: research

Applied microbiology of the phyllosphere
Appl Microbiol Biotechnol. 2024 Feb 15;108(1):211. doi: 10.1007/s00253-024-13042-4.ABSTRACTThe phyllosphere, or plant leaf surface, represents a microbial ecosystem of considerable size, holding extraordinary biodiversity and enormous potential for the discovery of new products, tools, and applications in biotechnology, agriculture, medicine, and elsewhere. This mini-review highlights the applied microbiology of the phyllosphere as an original field of study concerning itself with the genes, gene products, natural compounds, and traits that underlie phyllosphere-specific adaptations and services that have commercial and ec...
Source: Applied Microbiology and Biotechnology - February 15, 2024 Category: Microbiology Authors: Lorena I Rangel Johan H J Leveau Source Type: research

A study on the association between gut microbiota, inflammation, and type 2 diabetes
Appl Microbiol Biotechnol. 2024 Feb 15;108(1):213. doi: 10.1007/s00253-024-13041-5.ABSTRACTType 2 diabetes mellitus (T2DM) was reported to be associated with impaired immune response and alterations in microbial composition and function. However, the underlying mechanism remains elusive. To investigate the association among retinoic acid-inducible gene-I-like receptors (RLRs) signaling pathway, intestinal bacterial microbiome, microbial tryptophan metabolites, inflammation, and a longer course of T2DM, 14 patients with T2DM and 7 healthy controls were enrolled. 16S rRNA amplicon sequencing and untargeted metabolomics were ...
Source: Applied Microbiology and Biotechnology - February 15, 2024 Category: Microbiology Authors: Nannan Liu Xuehua Yan Bohan Lv Yanxiang Wu Xuehong Hu Chunyan Zheng Siyu Tao Ruxue Deng Jinfang Dou Binfang Zeng Guangjian Jiang Source Type: research