Applied Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Tailor-made solvents for microbial carotenoids recovery
Appl Microbiol Biotechnol. 2024 Feb 24;108(1):234. doi: 10.1007/s00253-024-13049-x.ABSTRACTIn recent years, microbial carotenoids have emerged as a promising alternative for the pharmaceutical and food industries, particularly in promoting human health due to their potent antioxidant and antimicrobial properties. Microbial carotenoids, particularly those produced by yeast, bacteria, and microalgae, are synthesized intracellularly, requiring the use of solvents for their effective extraction and recovery. The conventional use of toxic volatile organic solvents (VOCs) like hexane, petroleum ether, and dimethyl sulfoxide in t...
Source: Applied Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Authors: Cassamo U Mussagy Henua U Hucke Nataly F Ramos Helena F Ribeiro Mariana B Alves Ahmad Mustafa Jorge F B Pereira Fabiane O Farias Source Type: research

Characterization of a recombinant Aspergillus niger GZUF36 lipase immobilized by ionic liquid modification strategy
In this study, a recombinant extracellular lipase from Aspergillus niger GZUF36 (PEXANL1) expressed in Pichia pastoris GS115 was immobilized on ionic liquid-modified magnetic nano ferric oxide (Fe3O4@SiO2@ILs) via electrostatic and hydrophobic interaction. The morphology, structure, and properties of Fe3O4@SiO2@ILs and immobilized PEXANL1 were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, x-ray diffraction, vibration sample magnetometer, and zeta potential analysis. Under optimized conditions, the immobilization efficiency and activity recovery of immobilized PEXANL1 were 52 ± 2% ...
Source: Applied Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Authors: Shuqi Xing Jia Long Wei Xie Chaocheng Luo Laping He Cuiqin Li Xuefeng Zeng Source Type: research

An M cell-targeting recombinant L. lactis vaccine against four H. pylori adhesins
Appl Microbiol Biotechnol. 2024 Feb 23;108(1):231. doi: 10.1007/s00253-024-13070-0.ABSTRACTThe acidic environment and enzyme degradation lead to oral vaccines often having little immune effect. Therefore, it is an attractive strategy to study an effective and safe oral vaccine delivery system that can promote gastrointestinal mucosal immune responses and inhibit antigen degradation. Moreover, the antigens uptake by microfold cells (M cells) is the determining step in initiating efficient immune responses. Therefore, M cell-targeting is one promising approach for enhancing oral vaccine potency. In the present study, an M ce...
Source: Applied Microbiology and Biotechnology - February 24, 2024 Category: Microbiology Authors: Furui Zhang Tianyi Shi Zhen Zhang Shue Wang Jing Liu Yonghong Li Xuequan Wang Kunmei Liu Le Guo Source Type: research

A new and promiscuous α/β hydrolase from Acinetobacter tandoii DSM 14970 < sup > T < /sup > inactivates the mycotoxin ochratoxin A
Appl Microbiol Biotechnol. 2024 Feb 23;108(1):230. doi: 10.1007/s00253-024-13073-x.ABSTRACTThe presence of ochratoxin A (OTA) in food and feed represents a serious concern since it raises severe health implications. Bacterial strains of the Acinetobacter genus hydrolyse the amide bond of OTA yielding non-toxic OTα and L-β-phenylalanine; in particular, the carboxypeptidase PJ15_1540 from Acinetobacter sp. neg1 has been identified as an OTA-degrading enzyme. Here, we describe the ability to transform OTA of cell-free protein extracts from Acinetobacter tandoii DSM 14970 T, a strain isolated from sludge plants, and also rep...
Source: Applied Microbiology and Biotechnology - February 23, 2024 Category: Microbiology Authors: Ana S ánchez-Arroyo Laura Plaza-Vinuesa Mar ía Claudia Abeijón-Mukdsi Blanca de Las Rivas Jos é Miguel Mancheño Rosario Mu ñoz Source Type: research

Construction of viral-based expression vectors for high-level production of human interferon alpha 2b in plants
Appl Microbiol Biotechnol. 2024 Feb 23;108(1):229. doi: 10.1007/s00253-024-13069-7.ABSTRACTHuman interferon (hINF) alpha 2b is clinically important pharmaceutical product included in combinatory therapy against chronic hepatitis C and B and complex therapy against several cancer diseases. Here, we created the genetic constructions, based on genome elements of potato virus X (PVX), carrying the infα2b gene for transient expression in plant cells. The created plasmid vector constructions were tested through Agrobacterium-mediated transient gene expression method in two plant species-Nicotiana benthamiana and Ocimum basilicu...
Source: Applied Microbiology and Biotechnology - February 23, 2024 Category: Microbiology Authors: Yana Sindarovska Mykola Kuchuk Source Type: research

Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens
Appl Microbiol Biotechnol. 2024 Feb 24;108(1):232. doi: 10.1007/s00253-024-13033-5.ABSTRACTBacterial outer membrane vesicles (OMVs) are nanosized spheroidal particles shed by gram-negative bacteria that contain biomolecules derived from the periplasmic space, the bacterial outer membrane, and possibly other compartments. OMVs can be purified from bacterial culture supernatants, and by genetically manipulating the bacterial cells that produce them, they can be engineered to harbor cargoes and/or display molecules of interest on their surfaces including antigens that are immunogenic in mammals. Since OMV bilayer-embedded com...
Source: Applied Microbiology and Biotechnology - February 23, 2024 Category: Microbiology Authors: Eric K Lei Aruba Azmat Kevin A Henry Greg Hussack Source Type: research

FvKex2 is required for development, virulence, and mycotoxin production in Fusarium verticillioides
Appl Microbiol Biotechnol. 2024 Feb 22;108(1):228. doi: 10.1007/s00253-024-13022-8.ABSTRACTFusarium verticillioides is one of the most important fungal pathogens causing maize ear and stalk rots, thereby undermining global food security. Infected seeds are usually unhealthy for consumption due to contamination with fumonisin B1 (FB1) mycotoxin produced by the fungus as a virulence factor. Unveiling the molecular factors that determine fungal development and pathogenesis will help in the control and management of the diseases. Kex2 is a kexin-like Golgi-resident proprotein convertase that is involved in the activation of so...
Source: Applied Microbiology and Biotechnology - February 22, 2024 Category: Microbiology Authors: Limin Wu Wenyin Bian Yakubu Saddeeq Abubakar Jiayi Lin Huijuan Yan Huan Zhang Zonghua Wang Changbiao Wu WonBo Shim Guo-Dong Lu Source Type: research

Xanthan: enzymatic degradation and novel perspectives of applications
Appl Microbiol Biotechnol. 2024 Feb 21;108(1):227. doi: 10.1007/s00253-024-13016-6.ABSTRACTThe extracellular heteropolysaccharide xanthan, synthesized by bacteria of the genus Xanthomonas, is widely used as a thickening and stabilizing agent across the food, cosmetic, and pharmaceutical sectors. Expanding the scope of its application, current efforts target the use of xanthan to develop innovative functional materials and products, such as edible films, eco-friendly oil surfactants, and biocompatible composites for tissue engineering. Xanthan-derived oligosaccharides are useful as nutritional supplements and plant defense ...
Source: Applied Microbiology and Biotechnology - February 21, 2024 Category: Microbiology Authors: Oksana V Berezina Sergey V Rykov Wolfgang H Schwarz Wolfgang Liebl Source Type: research

The microbial biosynthesis of noncanonical terpenoids
In conclusion, the insights gathered from this review provide a reference for understanding the biosynthesis of these noncanonical terpenes and pave the way for the discovery of additional unique and novel noncanonical terpenes. KEY POINTS: •The activities and applications of noncanonical terpenoids are introduced. •The noncanonical terpenoids with irregular carbon skeletons are presented. •The microbial biosynthesis of noncanonical terpenoids is summarized.PMID:38381229 | PMC:PMC10881772 | DOI:10.1007/s00253-024-13048-y (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - February 21, 2024 Category: Microbiology Authors: Mengyu Ma Mingkai Li Zhenke Wu Xiqin Liang Qiusheng Zheng Defang Li Guoli Wang Tianyue An Source Type: research

The microbial biosynthesis of noncanonical terpenoids
In conclusion, the insights gathered from this review provide a reference for understanding the biosynthesis of these noncanonical terpenes and pave the way for the discovery of additional unique and novel noncanonical terpenes. KEY POINTS: •The activities and applications of noncanonical terpenoids are introduced. •The noncanonical terpenoids with irregular carbon skeletons are presented. •The microbial biosynthesis of noncanonical terpenoids is summarized.PMID:38381229 | DOI:10.1007/s00253-024-13048-y (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - February 21, 2024 Category: Microbiology Authors: Mengyu Ma Mingkai Li Zhenke Wu Xiqin Liang Qiusheng Zheng Defang Li Guoli Wang Tianyue An Source Type: research