Applied Microbiology and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Unveiling the repressive mechanism of a PPS-like regulator (PspR) in polyhydroxyalkanoates biosynthesis network
In this study, we demonstrated the specific function of the PPS-like protein as a negative regulator of phaR gene expression and PHBV synthesis. Chromatin immunoprecipitation (ChIP), in situ fluorescence reporting system, and in vitro electrophoretic mobility shift assay (EMSA) showed that the PPS-like protein can bind to the promoter region of phaRP. Computational modeling revealed a high structural similarity between the rifampin phosphotransferase (RPH) protein and the PPS-like protein, which has a conserved ATP-binding domain, a His domain, and a predicted DNA-binding domain. Key residues within this unique DNA-binding...
Source: Applied Microbiology and Biotechnology - March 18, 2024 Category: Microbiology Authors: Junyu Chen Yinglu Cui Shengjie Zhang Bian Wu Jing Han Hua Xiang Source Type: research
Versatile ferrous oxidation-xylenol orange assay for high-throughput screening of lipoxygenase activity
This study aimed to enhance the suitability of the ferrous-oxidized xylenol orange (FOX) assay for screening LOX activity across a wide pH range with different PUFAs. The narrow linear detection range of the standard FOX assay restricts its utility in screening LOX activity. To address this, the concentration of perchloric acid in the xylenol orange reagent was adjusted. The modified assay exhibited a fivefold expansion in the linear detection range for hydroperoxides and accommodated samples with pH values ranging from 3 to 10. The assay could quantify various hydroperoxide species, indicating its applicability in assessi...
Source: Applied Microbiology and Biotechnology - March 18, 2024 Category: Microbiology Authors: Ruth Chrisnasari Tom A Ewing Roelant Hilgers Willem J H van Berkel Jean-Paul Vincken Marie Hennebelle Source Type: research
Characterization of the ADP- β-D-manno-heptose biosynthetic enzymes from two pathogenic Vibrio strains
Appl Microbiol Biotechnol. 2024 Mar 18;108(1):267. doi: 10.1007/s00253-024-13108-3.ABSTRACTADP-activated β-D-manno-heptoses (ADP-β-D-manno-heptoses) are precursors for the biosynthesis of the inner core of lipopolysaccharide in Gram-negative bacteria. Recently, ADP-D-glycero-β-D-manno-heptose (ADP-D,D-manno-heptose) and its C-6'' epimer, ADP-L-glycero-β-D-manno-heptose (ADP-L,D-manno-heptose), were identified as potent pathogen-associated molecular patterns (PAMPs) that can trigger robust innate immune responses. Although the production of ADP-D,D-manno-heptose has been studied in several different pathogenic Gram-nega...
Source: Applied Microbiology and Biotechnology - March 18, 2024 Category: Microbiology Authors: Zhaoxiang Shi Yue Tang Zhenyi Wang Min Wang Zijian Zhong Jingming Jia Yihua Chen Source Type: research
Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution
This study aimed to breed thermotolerant Y. lipolytica and investigate the mechanism underlying the thermotolerant phenotype. Yarrowia lipolytica HT34, Yarrowia lipolytica HT36, and Yarrowia lipolytica HT385 that were capable of growing at 34 °C, 36 °C, and 38.5 °C, respectively, were obtained within 150 days (352 generations) by adaptive laboratory evolution (ALE) integrated with 60Co-γ radiation and ultraviolet ray radiation. Comparative genomics analysis showed that genes involved in signal transduction, transcription, and translation regulation were mutated during adaptive evolution. Further, we demonstrated that t...
Source: Applied Microbiology and Biotechnology - March 15, 2024 Category: Microbiology Authors: Kai Xia Yuqing Chen Fangmei Liu Xuequn Zhao Ruyi Sha Jun Huang Source Type: research
Cyanophycin modifications for applications in tissue scaffolding
Appl Microbiol Biotechnol. 2024 Mar 15;108(1):264. doi: 10.1007/s00253-024-13088-4.ABSTRACTCyanophycin (CGP) is a polypeptide consisting of amino acids-aspartic acid in the backbone and arginine in the side chain. Owing to its resemblance to cell adhesive motifs in the body, it can be considered suitable for use in biomedical applications as a novel component to facilitate cell attachment and tissue regeneration. Although it has vast potential applications, starting with nutrition, through drug delivery and tissue engineering to the production of value-added chemicals and biomaterials, CGP has not been brought to the indus...
Source: Applied Microbiology and Biotechnology - March 15, 2024 Category: Microbiology Authors: Natalia Kwiatos Deniz Atila Micha ł Puchalski Vignesh Kumaravel Alexander Steinb üchel Source Type: research
Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution
This study aimed to breed thermotolerant Y. lipolytica and investigate the mechanism underlying the thermotolerant phenotype. Yarrowia lipolytica HT34, Yarrowia lipolytica HT36, and Yarrowia lipolytica HT385 that were capable of growing at 34 °C, 36 °C, and 38.5 °C, respectively, were obtained within 150 days (352 generations) by adaptive laboratory evolution (ALE) integrated with 60Co-γ radiation and ultraviolet ray radiation. Comparative genomics analysis showed that genes involved in signal transduction, transcription, and translation regulation were mutated during adaptive evolution. Further, we demonstrated that t...
Source: Applied Microbiology and Biotechnology - March 15, 2024 Category: Microbiology Authors: Kai Xia Yuqing Chen Fangmei Liu Xuequn Zhao Ruyi Sha Jun Huang Source Type: research
Cyanophycin modifications for applications in tissue scaffolding
Appl Microbiol Biotechnol. 2024 Mar 15;108(1):264. doi: 10.1007/s00253-024-13088-4.ABSTRACTCyanophycin (CGP) is a polypeptide consisting of amino acids-aspartic acid in the backbone and arginine in the side chain. Owing to its resemblance to cell adhesive motifs in the body, it can be considered suitable for use in biomedical applications as a novel component to facilitate cell attachment and tissue regeneration. Although it has vast potential applications, starting with nutrition, through drug delivery and tissue engineering to the production of value-added chemicals and biomaterials, CGP has not been brought to the indus...
Source: Applied Microbiology and Biotechnology - March 15, 2024 Category: Microbiology Authors: Natalia Kwiatos Deniz Atila Micha ł Puchalski Vignesh Kumaravel Alexander Steinb üchel Source Type: research
Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution
This study aimed to breed thermotolerant Y. lipolytica and investigate the mechanism underlying the thermotolerant phenotype. Yarrowia lipolytica HT34, Yarrowia lipolytica HT36, and Yarrowia lipolytica HT385 that were capable of growing at 34 °C, 36 °C, and 38.5 °C, respectively, were obtained within 150 days (352 generations) by adaptive laboratory evolution (ALE) integrated with 60Co-γ radiation and ultraviolet ray radiation. Comparative genomics analysis showed that genes involved in signal transduction, transcription, and translation regulation were mutated during adaptive evolution. Further, we demonstrated that t...
Source: Applied Microbiology and Biotechnology - March 15, 2024 Category: Microbiology Authors: Kai Xia Yuqing Chen Fangmei Liu Xuequn Zhao Ruyi Sha Jun Huang Source Type: research
Cyanophycin modifications for applications in tissue scaffolding
Appl Microbiol Biotechnol. 2024 Mar 15;108(1):264. doi: 10.1007/s00253-024-13088-4.ABSTRACTCyanophycin (CGP) is a polypeptide consisting of amino acids-aspartic acid in the backbone and arginine in the side chain. Owing to its resemblance to cell adhesive motifs in the body, it can be considered suitable for use in biomedical applications as a novel component to facilitate cell attachment and tissue regeneration. Although it has vast potential applications, starting with nutrition, through drug delivery and tissue engineering to the production of value-added chemicals and biomaterials, CGP has not been brought to the indus...
Source: Applied Microbiology and Biotechnology - March 15, 2024 Category: Microbiology Authors: Natalia Kwiatos Deniz Atila Micha ł Puchalski Vignesh Kumaravel Alexander Steinb üchel Source Type: research
Roles of pH and phosphate in rare earth element biosorption with living acidophilic microalgae
Appl Microbiol Biotechnol. 2024 Mar 14;108(1):262. doi: 10.1007/s00253-024-13068-8.ABSTRACTThe increasing demand for rare earth elements (REEs) has spurred interest in the development of recovery methods from aqueous waste streams. Acidophilic microalgae have gained attention for REE biosorption as they can withstand high concentrations of transition metals and do not require added organic carbon to grow, potentially allowing simultaneous sorption and self-replication of the sorbent. Here, we assessed the potential of Galdieria sulphuraria for REE biosorption under acidic, nutrient-replete conditions from solutions contain...
Source: Applied Microbiology and Biotechnology - March 14, 2024 Category: Microbiology Authors: Jens Kastenhofer Oliver Spadiut Vladimiros G Papangelakis D Grant Allen Source Type: research
Pharmacokinetics and pharmacodynamics of antibacterial peptide NZX in Staphylococcus aureus mastitis mouse model
This study aimed to evaluate pharmacokinetics and pharmacodynamics of NZX against S. aureus-induced mouse mastitis. NZX exhibited potent in vitro antibacterial activity against the test S. aureus strains (minimal inhibitory concentration (MIC): 0.23-0.46 μM), low mutant prevention concentration (MPC: 1.18-3.68 μM), and a long post antibiotic effect (PAE: 2.20-8.84 h), which was superior to those of lincomycin and ceftiofur. Antibacterial mechanisms showed that NZX could penetrate the cell membrane, resulting in obvious cell membrane perforation and morphological changes, and bind to intracellular DNA. Furthermore, NZX ha...
Source: Applied Microbiology and Biotechnology - March 13, 2024 Category: Microbiology Authors: Xueling Zheng Na Yang Ruoyu Mao Ya Hao Da Teng Jianhua Wang Source Type: research
Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection
Appl Microbiol Biotechnol. 2024 Mar 13;108(1):261. doi: 10.1007/s00253-024-13102-9.ABSTRACTNon-coding RNA (ncRNA) plays a vital part in the regulation of immune responses, growth, and development in plants and animals. Here, the identification, characteristic analysis, and molecular verification of circRNAs in Apis cerana cerana worker larval guts were conducted, followed by in-depth investigation of the expression pattern of larval circRNAs during Ascosphaera apis infection and exploration of the potential regulatory part of differentially expressed circRNAs (DEcircRNAs) in host immune responses. A total of 3178 circRNAs ...
Source: Applied Microbiology and Biotechnology - March 13, 2024 Category: Microbiology Authors: Rui Guo Kaiyao Zhang He Zang Sijia Guo Xiaoyu Liu Xin Jing Yuxuan Song Kunze Li Ying Wu Haibing Jiang Zhongmin Fu Dafu Chen Source Type: research
Pharmacokinetics and pharmacodynamics of antibacterial peptide NZX in Staphylococcus aureus mastitis mouse model
This study aimed to evaluate pharmacokinetics and pharmacodynamics of NZX against S. aureus-induced mouse mastitis. NZX exhibited potent in vitro antibacterial activity against the test S. aureus strains (minimal inhibitory concentration (MIC): 0.23-0.46 μM), low mutant prevention concentration (MPC: 1.18-3.68 μM), and a long post antibiotic effect (PAE: 2.20-8.84 h), which was superior to those of lincomycin and ceftiofur. Antibacterial mechanisms showed that NZX could penetrate the cell membrane, resulting in obvious cell membrane perforation and morphological changes, and bind to intracellular DNA. Furthermore, NZX ha...
Source: Applied Microbiology and Biotechnology - March 13, 2024 Category: Microbiology Authors: Xueling Zheng Na Yang Ruoyu Mao Ya Hao Da Teng Jianhua Wang Source Type: research
Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection
Appl Microbiol Biotechnol. 2024 Mar 13;108(1):261. doi: 10.1007/s00253-024-13102-9.ABSTRACTNon-coding RNA (ncRNA) plays a vital part in the regulation of immune responses, growth, and development in plants and animals. Here, the identification, characteristic analysis, and molecular verification of circRNAs in Apis cerana cerana worker larval guts were conducted, followed by in-depth investigation of the expression pattern of larval circRNAs during Ascosphaera apis infection and exploration of the potential regulatory part of differentially expressed circRNAs (DEcircRNAs) in host immune responses. A total of 3178 circRNAs ...
Source: Applied Microbiology and Biotechnology - March 13, 2024 Category: Microbiology Authors: Rui Guo Kaiyao Zhang He Zang Sijia Guo Xiaoyu Liu Xin Jing Yuxuan Song Kunze Li Ying Wu Haibing Jiang Zhongmin Fu Dafu Chen Source Type: research
Computational aptamer design for spike glycoprotein (S) (SARS CoV-2) detection with an electrochemical aptasensor
Appl Microbiol Biotechnol. 2024 Mar 12;108(1):259. doi: 10.1007/s00253-024-13066-w.ABSTRACTA new bioinformatic platform (APTERION) was used to design in a short time and with high specificity an aptamer for the detection of the spike protein, a structural protein of SARS-CoV-2 virus, responsible for the COVID-19 pandemic. The aptamer concentration on the carbon electrode surface was optimized using static contact angle and fluorescence method, while specificity was tested using differential pulse voltammetry (DPV) associated to carbon screen-printed electrodes. The data obtained demonstrated the good features of the aptame...
Source: Applied Microbiology and Biotechnology - March 12, 2024 Category: Microbiology Authors: Alessia Cossettini Laura Pasquardini Antonello Romani Aldo Feriani Debora Pinamonti Marisa Manzano Source Type: research
